Article Per Year (5 Year)
p-Index From 2021 - 2026
2.258
P-Index
This Author published in this journals
All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal Bioma : Jurnal Biologi Indonesia Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Jurnal Agripet Animal Production Biodiversitas Journal of Biological Diversity
Asep Gunawan
Department Of Animal Production And Technology, Faculty Of Animal Science, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

Published : 34 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 34 Documents
Search

 1   2   3   4 
Association of DGAT1 Gene Related to Flavor, Odor, Cholesterol, and Mineral in Indonesian Sheep Fisma Amri; Ratna Sholatia Harahap; Cece Sumantri; Ismeth Inounu; Depison Depison; Yun Alwi; Asep Gunawan
Jurnal Ilmu Ternak dan Veteriner Vol 28, No 2 (2023): June 2023
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14334/jitv.v28i2.3152

Abstract

Diacylglycerol acyltransferase 1 (DGAT1) is a potential candidate gene for improving Indonesian lamb’s quality and nutrient value.  The study aimed to identify the diversity of the DGAT1 gene with SNP g.8539 CT and its relation to Indonesian lamb's flavor and odor, cholesterol, and mineral.  Total of 254 ten to twelve months old sheep consisted of 20 Javanese fat-tail sheep (JFTS), 107 Javanese thin-tail sheep (JTTS), 10 Garut composite sheep (GCS), 10 Compass agrinak sheep (CAS), 10 Barbados cross sheep (BCS), 20 Garut sheep (GS), 27 Jonggol sheep (JS), and 50 Jambi local sheep (JLS).  One hundred sheep were used to analyze the association of the DGAT1 gene with flavor and odor content, cholesterol, and mineral.  The diversity of DGAT1|AluI was analyzed with the PCR-RFLP method.  The association of the DGAT1 gene with the nutritional value of meat was analyzed using the GLM (General Linear Model) method.  The results showed that the DGAT gene was polymorphic in JTTS, GS, and JFTS and monomorphic in CAS, BCS, JLS, and GCS.  CC and CT genotypes were found in JTTS, GS, and JFTS.  SNP g.8539 CT of DGAT1 gene had a significant association (P0.05) with flavor and odor, 4-Ethyloctanoic Acid (EOA).  However, the DGAT1 gene had no significant association (P0.05) with cholesterol and mineral.  The DGAT1 gene might be marker-assisted selection for improving lamb flavor and odor in Indonesian sheep.
Characterization and Association of CFHR5 Gene Polymorphism with Fatty Acid Composition and Cholesterol in Sheep Akbanugra Yudhananda; Kasita Listyarini; Cece Sumantri; Asep Gunawan
Buletin Peternakan Vol 47, No 3 (2023): BULETIN PETERNAKAN VOL. 47 (3) AUGUST 2023
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v47i3.84177

Abstract

The Complement Factor H Related 5 (CFHR5) gene is speculated to have an important role in regulating fatty acid composition in sheep. The aim of this study was to investigate the polymorphism of the CFHR5 gene and its association with fatty acid composition and cholesterol of sheep in Indonesia. A total of 172 rams from 83 priangan sheep (PS), 20 sapudi sheep (SS), 19 Garut sheep (GS), 20 jonggol sheep (JS), 10 Garut composite sheep (GCS), 10 compass agrinac sheep (CAS), and 10 Barbados cross sheep (BCS) were used for this study. Identification of CFHR5 gene polymorphism were performed by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) using AciI restriction enzyme. The results showed that the CFHR5 gene in all sheep populations were polymorphic producing three genotypes, e.g. CC, CT and TT, respectively. The polymorphism had a significant effect (p<0.05) on fatty acid composition (miristoleic [C14:1], ginkgolic [C17:1], tridecanoic [C13:0], and heptadecanoic [C17:0] acids) and cholesterol. The breed and polymorphism had a significant effect (p<0.05) on fatty acid composition (JS with tridecanoic acid [C13:0] and BCS with miristoleic acid [C14:1]). The CC genotype is the preferred genotype and as it exhibits reduced levels of saturated fatty acids and cholesterol. The BCS sheep is the preferred genotype and as it exhibits high PUFA/SFA ratio. The CFHR5 gene (SNP c.1011C>T) has the potential to be used as a genetic marker for the selection of low saturated fatty acid composition and cholesterol in sheep
Evaluasi Keragaman Gen Selenoprotein W1 (SEPW1) dan Kaitannya Terhadap Komposisi Asam Lemak Pada Domba Yusuf, Salman; Harahap, Ratna Sholatia; Listyarini, Kasita; Rahayu, Sri; Sumantri, Cece; Gunawan, Asep
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 9, No 1 (2022): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.109 KB) | DOI: 10.33772/jitro.v9i1.17717

Abstract

Kandungan asam lemak dipengaruhi oleh sejumlah gen dan di antaranya ialah selenoprotein W1 (SEPW1). Eksperimen ini bermaksud untuk mengetahui polimorfisme gen SEPW1 serta hubungannya terhadap kandungan asam lemak di domba. Sejumlah 102 ekor domba yang tersusun dari 20 domba ekor gemuk (DEG), 30 domba ekor tipis (DET), 9 domba komposit garut (DKG), 10 domba compass agrinak (DCA), 10 domba barbados cross (DBC), dan 23 domba jonggol (DJ) digunakan dalam penelitian ini. Analisis polimorfisme gen SEPW1 dicari dengan memanfaatkan Polymerase Chain Reaction-Restriction Fragment Lenght Polymorphism (PCR-RFLP), enzim BanI, serta produk amplifikasi berukuran 478 bp. Analisis Chi-square memberikan hasil bahwa populasi berada dalam keseimbangan Hardy-Weinberg, kecuali sampel domba jonggol. Polimorfisme gen SEPW1 teramati berada di semua sampel rumpun domba. Single nucleotide polymorphism (SNP) g.53617063 G>A gen SEPW1 berasosiasi (P<0,05) dengan asam laurat (C12:0), asam palmitat (C16:0), asam arakidat (C20:0), asam henekosilat (C21:0), asam miristoleinat (C14:1), asam oleat (C18:1n9c), asam linoelat (C18:2n6c) dan asam lemak total. Genotipe GG memiliki kandungan asam lemak tak jenuh yang paling tinggi dibandingkan genotipe lainnya. Gen SEPW1 memiliki kapasitas untuk dikembangkan sebagai penanda asam lemak di domba.
Selenoprotein W1 (SEPW1) Gene Polymorphism and Association with Mineral Content in Sheep Astira, Adinda Wanda; Harahap, Ratna Sholatia; Sumantri, Cece; Gunawan, Asep
Jurnal Ilmu dan Teknologi Peternakan Tropis Vol 10, No 1 (2023): JITRO, January
Publisher : Universitas Halu Oleo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33772/jitro.v10i1.27066

Abstract

Selenoprotein W1 (SEPW1) is a protein containing selenium that has an important role in muscle growth and development by protecting myoblasts from oxidative stress. This study was aim to identify the diversity of the SEPW1 gene and its association with mineral content in sheep. The samples used were 100 sheep consisting of 10 samples of compass agrinak sheep (CAS), 15 samples of Javanese fat-tailed sheep (JFT), 30 samples of Javanese thin-tailed sheep (JTT), 25 samples of garut composite sheep (GCS), and 20 samples of jonggol sheep (JS). Selected mineral contents were determined using the IK method LP-04.10-LT-1.0. Identification of SEPW1 gene polymorphism will be conducted using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). The size of the amplification product was 455 bp. Chi-square analysis showed that the population was in Hardy-Weinberg equilibrium. Polymorphism of the SEPW1 gene was found in each breed of sheep. The SEPW1 gene’s SNP g.53617063 A>G was significantly (p
Determining the Matrilineal Origin of Indonesian Kerinci Duck Breed (Anas platyrhynchos) Based on MT-ND2 Gene Diversity Depison; Putra, Widya Pintaka Bayu; Gushairiyanto; Gunawan, Asep; Sumantri, Cece
HAYATI Journal of Biosciences Vol. 31 No. 3 (2024): May 2024
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.31.3.581-588

Abstract

The Kerinci duck (Anas platyrhynchos), hailing from Jambi Province, represents one of the local duck breeds of Indonesia. This study aimed to ascertain the matrilineal origin of Kerinci ducks through analysis of the mitochondrial NADH Dehydrogenase subunit 2 (MT-ND2) gene. In this pursuit, forward sequences of the MT-ND2 gene (490 bp) were extracted from unsexed Kerinci ducks, totalling forty-eight (48) sequences, procured from blood samples. The findings demonstrated the identification of fifteen (15) distinct haplotypes within the MT-ND2 gene, with corresponding haplotype diversity (Hd) and nucleotide diversity (pi) values of 0.74 and 0.003, respectively. The resulting phylogenetic tree unveiled that Kerinci ducks exhibit two matrilineal origins: an Asian and an independent Kerinci lineage. Moreover, most Kerinci ducks were categorised within the H4 group (24 birds) of the Asian lineage. Nevertheless, this study also revealed the existence of an independent Kerinci lineage comprising eight (8) duck haplotypes. In conclusion, the analysis of the MT-ND2 gene underscored the genetic introgression of A. zonorhyncha and A. poecilorhyncha in Kerinci ducks.
Polymorphism and Expression of HSD17β13 Gene and Its Association with Lamb Quality of Indonesian Sheep Ratna Sholatia Harahap; Ronny Rachman Noor; Asep Gunawan
ANIMAL PRODUCTION Vol. 23 No. 1 (2021)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2021.23.1.88

Abstract

HSD17β13 (17beta 13-Hydroxysteroid dehydrogenases) are important enzymes in steroid metabolism. This study investigated the polymorphisms and expression of the HSD17β13 gene in lamb quality traits in Indonesian sheep. A total of 200 of rams of seven breeds administered in the study were Javanese fat-tailed (JFT), Javanese thin-tailed (JTT), Garut sheep (GS), Jonggol sheep (JS), Garut composite sheep (GCS), Compass agrinac sheep (CAS), Barbados cross sheep (BCS) aged 10-12 months and weighed 20-30 kg. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and MspI enzyme restriction were performed to identify the polymorphism of the HSD17β13 gene. The lamb quality traits were measured for physical quality, flavor, odor, and fatty acid composition. The results showed that the HSD17β13 gene was polymorphic and had three genotypes i.e., CC, CT, and TT. The highest genotype frequency was the CC when compared to the other genotypes. The allele frequency of HSD17β13 gene was not in Hardy Weinberg Equilibrium. The polymorphism of HSD17β13 gene was significantly (P<0.05) associated with the fatty acid composition and Saturated Fatty Acid (SFA), i.e., tridecanoic acid (C13:0), arachidic acid (C20:0), henecosanoic acid (C21:0), and tricosanoic acid (C23:0). The polymorphism was also significantly associated with the Monounsaturated Fatty Acid (MUFA) i.e. ginkgoleic acid (C17:1) and nervonoic acid (C24:1), and Polyunsaturated Fatty Acid (PUFA) i.e. eicosedienoic acid (C20:2). The level of expression of the HSD17β13 gene based on quantitative real time-PCR analysis results was not significantly different (P>0.05) among genotypes for lamb quality traits. It can be concluded that the polymorphism of HSD17β13 gene was associated with the fatty acid composition of lamb in Indonesian sheep.
Pengaruh Polimorfisme Gen Heat Shock Protein 70 (HSP70) SacII terhadap Toleransi Panas Itik Lokal Sumatera Barat Kusnadidi Subekti; Dedi Duryadi Solihin; Rudi Afnan; Asep Gunawan; Cece Sumanri
Jurnal Agripet Vol 19, No 2 (2019): Volume 19, No. 2, Oktober 2019
Publisher : Agricultural Faculty

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17969/agripet.v19i2.14876

Abstract

ABSTRAK. Tujuan dari penelitian ini adalah untuk mengidentifikasi pengaruh polimorfisme gen heat shock protein 70 (HSP70) terhadap sifat toleransi panas. Genotiping dilakukan pada 110 itik lokal dari 4 populasi itik Sumatera Barat (pitalah, bayang, kamang, dan payakumbuh). Enzim restriksi yang digunakan adalah SacII. Cekaman panas dilakukan selama 1 jam dengan sampel itik lokal sebanyak 24 ekor untuk menganalisa status hematologi. PCR-RFLP dan Sanger sequencing assay digunakan untuk mengidentifikasi polimorfisme. Analisis polimorfisme dilakukan dengan menggunakan Software MEGA 7 dan pengaruhnya dianalisis dengan ANOVA menggunakan software SAS 9.4. Produk amplifikasi yang dihasilkan yaitu 466 pasang basa. Lokus HSP70/SacII mengenali satu situs SNP (Single nucleotide Polymorphism) g.1702TC, menghasilkan dua alel (T dan C) dan tiga genotype (TT, TC, CC). Gen HSP70/SacII bersifat polimorfik pada semua populasi. Hasil penelitian menunjukkan bahwa alel T memiliki frekuensi yang lebih tinggi daripada alel C pada semua populasi. Analisis chi-kuadrat (2) menunjukkan bahwa semua populasi itik lokal memenuhi kaidah kesetimbangan Hardy-Weinberg. Pengaruh genotipe gen HSP70 terhadap status hematologi menunjukkan perbedaan (P0,05) terhadap nilai heterofil dan nilai H/L dengan genotipe CT menunjukkan toleransi panas yang lebih baik dibanding genotipe lainnya. Gen HSP70/SacII dapat dijadikan marka molekuler sifat toleransi panas pada itik lokal berdasarkan keragaman dan pengaruhnya terhadap status hematologi.(The effect of heat shock protein 70 (HSP70) SacII gene polymorphism on heat tolerance of West Sumatera local duck)ABSTRACT. The objective of this study was to identify the effect of HSP70/SacII gene polymorphism associated with the thermotolerance trait. Genotyping was performed on 110 local duck from the West Sumatera ducks population (pitalah, bayang, kamang, and payakumbuh). The restriction enzyme used was SacII. Heat stress was done for 1 hour with samples used as much as 24 birds to analyze hematological status. PCR-RFLP and Sanger sequencing assays were used to identify polymorphism. Analysis of polymorphism was conduct by MEGA 7 software and its effect is analyzed with ANOVA using Statistical SAS 9.4 software. The product of amplification was 466 bp. HSP70/SacII recognized one SNP g.1702TC, producing two alleles (T and C) and three genotypes (TT,TC,CC). The HSP70/SacII locus were polymorphic in all population. T allele had a higher frequency than C allele in all populations. The analysis of chi-square (2) showed that all local ducks population were fit with Hardy-Weinberg equilibrium. The effect of HSP70/SacII gene on hematological status showed differences (P0,05) of heterophil and H/L ratio with CT genotype show better heat tolerance than other genotypes. HSP70/SacII gene can be used as a marker of heat tolerance in local ducks based on polymorphism and its effect on hematological status.
Polymorphism of PPM1K Gene and The Association Related to Retail Indonesian Sheep Meat Cuts Suhendra, Muhammad; Ratna Sholatia Harahap; Kasita Listyarini; Cece Sumantri; Cahyo Budiman; Katrin Roosita; Gunawan, Asep
ANIMAL PRODUCTION Vol. 26 No. 1 (2024)
Publisher : Faculty of Animal Science, Jenderal Soedirman University in associate with the Animal Scientist Society of Indonesia (ISPI) and the Indonesian Association of Nutrition and Feed Science (AINI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.jap.2024.26.1.216-1

Abstract

The PPM1K (Protein Phosphatase, Mg2+/Mn2+ Dependent 1K) gene is assumed to associate with retail meat cuts. This study aims to determine the association of the PPM1K gene polymorphisms with retail meat cuts among various breeds of Indonesian sheep. The Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) approach was used to determine the polymorphisms of the PPM1K gene in 130 Indonesian rams aged 10-12 months with body weights ranging from 20-25 kg. The association study between PPM1K and retail meat cuts was analyzed using General Linear Model (GLM). The research showed that the PPM1K gene was polymorphic, with three genotypes: AA (458 bp), GG (259 bp and 199 bp), and AG (458 bp, 259 bp, and 199 bp). The PPM1K gene polymorphisms were significantly (P<0.05) associated with breast cut, which is the breast muscle and bone. The GG genotype of the PPM1K gene had a higher value on breast lamb. This result implies that the PPM1K gene could be a potential candidate for marker-assisted selection for improving the lamb quality of Indonesian sheep.
Identification of AHSG gene polymorphism and association with flavor and odor compounds of Indonesian lamb meat Septiyawan, Ganda Adi; Listyarini, Kasita; Noor, Ronny Rachman; Roosita, Katrin; Gunawan, Asep
Buletin Peternakan Vol 49, No 1 (2025): BULETIN PETERNAKAN VOL. 49 (1) FEBRUARY 2025
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v49i1.89123

Abstract

Flavor and odor are important factors for consumers to consider the quality of lamb meat. The AHSG Alpha2-Heremans- Schmid Glycoprotein gene is associated with a fatty acid content and is indicated as a gene controlling flavor and odor compounds. The research aims to identify the AHSG gene polymorphism and its association with flavor and odor compounds in Indonesian lamb meat. The sample used in this study was 105 ram samples consisting of 10 Javanese Fat-Tailed Sheep (JFTS), 80 Javanese Thin-Tailed Sheep (JTTS), and 15 Jonggol sheep (JS). The AHSG gene polymorphism was validated using the PCR-RFLP technique with the EagI enzyme EagI. The association of the AHSG gene SNP (g.198655287 G>A) with odor and flavor was analyzed using the T-tests. The AHSG gene polymorphism in Indonesian lamb was polymorphic with three genotypes, i.e., GG, GA, and AA, and in the Hardy-Weinberg equilibrium. The AHSG significance was associated (P<0.05) with skatole compounds (MOA, EOA, MNA, and MI). The AA genotype is recommended for a candidate marker of flavors and odors because it associated with low compound values. The AHSG gene, specifically the SNP g.198655287 G>A, can potentially serve as a genetic marker for lamb meat production by reducing flavor and odor.
Identifikasi Keragaman Gen Myostatin (MSTN|BsrI) Pada Ayam Kampung Dengan Metode PCR-RFLP Shidiq, Fajrin; Yulianto, Yulianto; Gunawan, Asep; Sumantri, Cece
TERNAK TROPIKA Journal of Tropical Animal Production Vol. 24 No. 1 (2023): TERNAK TROPIKA Journal of Tropical Animal Production
Publisher : Jurusan Produksi Ternak, Fakultas Peternakan, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/jtapro.2023.024.01.8

Abstract

Myostatin (MSTN) atau Growth and Differentiation Factor 8 (GDF8) adalah anggota dari superfamili Transforming Growth Factor (TGF)-β yang bertindak sebagai pengatur negatif pertumbuhan otot rangka. Mutasi pada ekson 2, di mana basa timin pada gen myostatin berubah menjadi basa guanin, mengubah asam amino leusin menjadi arginin, yang mempengaruhi berat badan ayam. Penelitian ini bertujuan untuk mengidentifikasi keragaman gen myostatin pada 132 ekor ayam kampung yang terdiri dari ayam kampung Ciawi dan kampung Sukabumi. Sebagai perbandingan keragaman pada populasi besar digunakan ayam broiler, nunukan dan petelur. Keragaman gen diidentifikasi menggunakan metode Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) menggunakan enzim restriksi BsrI. Produk amplifikasi sepanjang 247 pb, genotipe yang ditemukan pada keragaman gen MSTN|BsrI adalah GG, GT dan TT. Hasil analisis menunjukkan bahwa frekuensi genotipe GT dan TT lebih tinggi dibandingkan dengan genotipe GG. Pada semua populasi frekuensi alel T lebih besar dari frekuensi alel G yaitu 0.72 dan 0.28. Gen MSTN|BsrI pada populasi ayam kampung Ciawi dan ayam broiler berada dalam kesetimbangan Hardy-Weinberg. Populasi ayam kampung Ciawi dan Sukabumi menunjukkan skor heterozigositas (Ho) lebih tinggi dari skor heterozigositas yang diharapkan (He), sedangkan ayam pedaging menunjukkan skor heterozigositas (Ho) sama dengan skor heterozigositas yang diharapkan (He). Gen MSTN|BsrI pada populasi Kampung Ciawi, Kampung Sukabumi dan ayam broiler bersifat polimorfik
Co-Authors A. Furqon A. S. Mentari Ahmad Furqon Akbanugra Yudhananda Amri, Fisma Anneke Anggraeni Astira, Adinda Wanda Bambang Purwantara C Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Talib Cahyo Budiman Cece Sumanri Cece Sumantri Chalid Talib D. Anggrela D. D. Solihin Dedi Duryadi Solihin Dedi Duryadi Solihin Dedy Duryadi Solihin Depison Depison Depison Depison DEWI APRI ASTUTI Ekayanti Mulyawati Kaiin Elmy mariana F Hasan Gushairiyanto Gushairiyanto Hanum Isfaeni Herpina Herpina Himmatul Khasanah I. Inounu Ismeth Inounu ISYANA KHAERUNNISA J. P. Munyaneza Jakaria Jakaria Junaedi Junaedi K. Listyarini K. Listyarini Katrin Roosita Kusnadidi Subekti Ligaya I.T.A Tumbelaka Linda Suhartati Listyarini, Kasita M. A. Abuzahra M. Ardiyana M. F. Al-Habib M. F. Ulum M. J. Uddin M. Yamin Ma'arif, Naufal Mohamad Yamin Mohamad Yamin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin Muhammad Agil Muhammad Gunawan Muhammad Suhendra, Muhammad N. Ulupi Naufal Ma'arif Niken Ulupi Novita Nur Anggraeni R R Noor R. Iis Arifiantini R. Priyanto R. Putri R. R. Noor R. R. Noor Ratna Sholatia Harahap Raudah Putri Ekawati RH Mulyono, RH Rizqan Rizqan Ronnie Permana Ronny Rachman Noor Rudi Afnan Rudy Priyanto RUKMIASIH RUKMIASIH S. Murtini S. Murtini S. Y. Basril Septiyawan, Ganda Adi Shidiq, Fajrin SRI DARWATI SRI RAHAYU T. Sartika Tuti Suryati, Tuti Vincentia Trisna Yoelinda Widya Pintaka Bayu Putra Woki Bilyaro Yulianto Yulianto Yun Alwi Yusuf, Salman Zulfi Nur Amrina Rosyada