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All Journal HAYATI Journal of Biosciences MEDIA PETERNAKAN - Journal of Animal Science and Technology Jurnal Ilmu Pertanian Indonesia Buletin Peternakan Jurnal Ilmu Ternak Veteriner Journal of the Indonesian Tropical Animal Agriculture Jurnal Ternak Tropika JITRO (Jurnal Ilmiah dan Teknologi Peternakan Tropis) Animal Production : Indonesian Journal of Animal Production Tropical Animal Science Journal Bioma : Jurnal Biologi Indonesia Jurnal Kedokteran Hewan Chalaza Journal of Animal Husbandry Jurnal Agripet Animal Production Biodiversitas Journal of Biological Diversity
Asep Gunawan
Department Of Animal Production And Technology, Faculty Of Animal Science, Bogor Agricultural University Jl. Agatis, Dramaga Campus, Bogor 16680
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Energy Environmental Science Immunology & microbiology Medicine & Pharmacology Veterinary Other

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THE EFFECTS OF EXERCISE MODELS ON HEMATOLOGICAL PROFILE AND MEET PHYSICAL QUALITY IN GARUT SHEEP Permana, Ronnie; Yamin, Mohamad; Priyanto, Rudy; Gunawan, Asep; Astuti, Dewi Apri
Jurnal Kedokteran Hewan Vol 14, No 4 (2020): December
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i4.16035

Abstract

The objective of this study was to evaluate the effect of exercise levels on the hematological and metabolical status, and carcass quality of Garut sheep. In total 24 of Garut sheep used in this study. The levels of exercise contain three treatments namely non exercise, semi exercise and exercise. Blood collection through the jugular vein of sheep samples was carried out at the end of the study, using a 5 mL EDTA tube to measure the haematological condition of the animal sample. Likewise, collecting muscle samples to determine the physical quality of meat.The data was analyzed by analysis of variance and continued by Duncan test for a post hoc test. The results showed that the models of exercise were not significantly (P0.05) affected to hematological level and also carccas quality including juiceness, tenderness, drip loss and water holding capacity. Organoleptic sensory using semiexercise was more favourable meat compare to exercise and non exercise. While for the colour of meat, non exercise treatment was significantly (P0.05) affected to the colour of meat compare to exercise and semi exercise. Furthermore the semi exercise was the best treatment to produce fresh taste meat with good sensory characteristic.
ANALISIS KEANEKARAGAMAN GEN MYOSTATIN (MSTN) SECARA IN-SILICO PADA CATTLE DI INDONESIA Naufal Ma'arif; Hanum Isfaeni; Asep Gunawan
Bioma Vol. 19 No. 1 (2023): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma19(1).3

Abstract

Cattle is one of the largest livestock products in Indonesia. The purpose of this study was to determine the diversity of the myostatin (MSTN) gene found in several cattle in Indonesia. This research was a descriptive analysis research based on several literature studies. Gene data was taken from the National Center for Biotechnology Information (NCBI). The gene data taken were from several species such as Bos indicus, Bos indicus×Bos taurus, and Bos taurus. Meanwhile, Bubalus bubalis, Capra hircus, and Cervis sp. were selected as outgroups. DNA sequence data analysis was aligned using MEGA XI software. MSTN gene parameters showed results between the kinship of several cattle in Indonesia. From these results it can be found that there were still several species of cattle in Indonesia that were not yet found in the NCBI database on the MSTN gene. For example, Madura cattle (Bos javanicus), PO Kebumen, PO Aceh, and several livestock products that do not yet have the MSTN gene characterization database. Therefore, to improve the quality of beef in Indonesia, it is necessary to characterize the MSTN gene in several species that are not yet in the database as a benchmark for the quality level of cattle in Indonesia.
Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken Linda Suhartati; ISYANA KHAERUNNISA; ASEP GUNAWAN; RUKMIASIH RUKMIASIH; SRI DARWATI; CECE SUMANTRI; RIZQAN RIZQAN
Biodiversitas Journal of Biological Diversity Vol. 21 No. 8 (2020)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d210860

Abstract

Abstract. Suhartati L, Khaerunnisa L, Gunawan A, Rukmiasih, Darwati S, Sumantri C, Rizqan. 2020. Short Communication: Identification of the exon 1 myostatin gene polymorphism and its association with slaughtered weight in Indonesian Kampung and Broiler Chicken. Biodiversitas 21: 3893-3897.  Myostatin is widely known as a negative regulator of skeletal muscle development by controlling hypertrophy and hyperplasia. Silent mutation in exon 1 (c.234G>A) was then thought to be associated with chicken growth performances. This study was designed to analyze polymorphisms of c.234G>A mutation of the myostatin gene and its effect on slaughtered weight in Indonesian Kampung and meat-type chicken. The SatI restriction enzyme was used for genotype determination through the PCR-RFLP technique. The effect of genotype on the slaughtered weight was analyzed using the General Linear Model (GLM) procedure. Genotyping was performed on 138 chicken from Kampung (n=67) and meat type (cobb Broiler) (n=71) chicken. The amplification product contained 226 bp nucleotides. This myostatin gene was polymorphic in both chicken population, produced 2 alleles (G and A), and 3 genotypes (GG, GA, and AA). The A allele had a higher frequency than the G allele in all populations. In this study, genotype of AA and GA had higher live weight, carcass weight, breast weight, thigh weight, drumsticks weight, wings weight, breast muscle weight, thighs muscle weight, drumsticks muscle weight than GG genotype of 12 weeks-old Kampung chicken. The results suggested that the c.234G>A mutation in exon 1 could be potentially recommended as a genetic marker for chicken slaughtered weight traits.
Sperm osteopontin mRNA expression levels and its correlation on semen quality and fertility in Madura bulls Zulfi Nur Amrina Rosyada; Vincentia Trisna Yoelinda; Ekayanti Mulyawati Kaiin; Muhammad Gunawan; Mokhamad Fakhrul Ulum; Ligaya I.T.A Tumbelaka; Dedy Duryadi Solihin; Muhammad Agil; Asep Gunawan; Bambang Purwantara
Biodiversitas Journal of Biological Diversity Vol. 24 No. 1 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240165

Abstract

Abstract. Rosyada ZNA, Yoelinda VT, Kaiin EM, Gunawan M, Ulum MF, Tumbelaka LITA, Solihin DD, Agil M, Gunawan A, Purwantara B. 2023. Sperm osteopontin mRNA expression levels and its correlation on semen quality and fertility in Madura bulls. Biodiversitas 24: 563-570. Osteopontin (OPN) gene transcripts influence spermatogenesis and germ cell development. Therefore, transcriptome analysis is needed to identify the fertility factor OPN in Madura bull sperm. Madura cattle are crosses originating from Bos indicus (zebu) and Bos javanicus (banteng). This study aims to examine sperm osteopontin (OPN) mRNA expression levels and its correlation with semen quality and fertility in Madura bulls. Frozen semen samples from Madura bulls were categorized as high-fertile (n: 4, average field conception rate: 78.28±3.25%) or sub-fertile (n: 4, average field conception rate: 66.73±5.01%). In post-thaw semen samples, sperm motility, viability, membrane, acrosome, and DNA fragmentation index were evaluated. OPN expression in sperm total RNA was analyzed using Real-Time Quantitative Polymerase Chain Reaction (RT-qPCR). The data analysis between the two fertility groups was assessed with Student's t-test and Pearson Square. Receiver-operating characteristic analysis and diagnostic sensitivity and specificity calculations were performed. Sperm motility, acrosome intact, and DFI differ (p<0.05) between groups, whereas viability and membrane plasma intact have no significance (p>0.05). In high-fertile Madura bulls, OPN mRNA was upregulated (p<0.05). The OPN mRNA expression had a strong correlation with the field conception rate (R: 0.807, P<0.05), sperm motility, and intact acrosome (R: 0.899, p<0.01; R: 0.804, p<0.05) respectively. In contrast, OPN negatively correlated with sperm DFI (R: -0.764, p<0.05). The OPN predicted bull fertility with 81.3% accuracy, 75% sensitivity, and 75% specificity. Thus, OPN may serve as a potential biomarker of Madura bull fertility.
Co-Authors A. Furqon A. S. Mentari Ahmad Furqon Akbanugra Yudhananda Amri, Fisma Anneke Anggraeni Astira, Adinda Wanda Bambang Purwantara C Sumantri C. Sumantri C. Sumantri C. Sumantri C. Sumantri C. Talib Cahyo Budiman Cece Sumanri Cece Sumantri Chalid Talib D. Anggrela D. D. Solihin Dedi Duryadi Solihin Dedi Duryadi Solihin Dedy Duryadi Solihin Depison Depison Depison Depison DEWI APRI ASTUTI Ekayanti Mulyawati Kaiin Elmy mariana F Hasan Gushairiyanto Gushairiyanto Hanum Isfaeni Herpina Herpina Himmatul Khasanah I. Inounu Ismeth Inounu ISYANA KHAERUNNISA J. P. Munyaneza Jakaria Jakaria Junaedi Junaedi K. Listyarini K. Listyarini Katrin Roosita Kusnadidi Subekti Ligaya I.T.A Tumbelaka Linda Suhartati Listyarini, Kasita M. A. Abuzahra M. Ardiyana M. F. Al-Habib M. F. Ulum M. J. Uddin M. Yamin Ma'arif, Naufal Mohamad Yamin Mohamad Yamin Mokhamad Fakhrul Ulum, Mokhamad Muh. Rusdin Muhammad Agil Muhammad Gunawan Muhammad Suhendra, Muhammad N. Ulupi Naufal Ma'arif Niken Ulupi Novita Nur Anggraeni R R Noor R. Iis Arifiantini R. Priyanto R. Putri R. R. Noor R. R. Noor Ratna Sholatia Harahap Raudah Putri Ekawati RH Mulyono, RH Rizqan Rizqan Ronnie Permana Ronny Rachman Noor Rudi Afnan Rudy Priyanto RUKMIASIH RUKMIASIH S. Murtini S. Murtini S. Y. Basril Septiyawan, Ganda Adi Shidiq, Fajrin SRI DARWATI SRI RAHAYU T. Sartika Tuti Suryati, Tuti Vincentia Trisna Yoelinda Widya Pintaka Bayu Putra Woki Bilyaro Yulianto Yulianto Yun Alwi Yusuf, Salman Zulfi Nur Amrina Rosyada