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All Journal Jurnal Sain Veteriner SAINS DAN MATEMATIKA Prosiding Seminar Biologi Health Science Journal of Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Urology agriTECH Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY BERITA BIOLOGI Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Universa Medicina Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Molekul: Jurnal Ilmiah Kimia Oto Rhino Laryngologica Indonesiana INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Narra J Proceeding Biology Education Conference Cermin Dunia Kedokteran Nutrisia Medical and Health Journal Jurnal Pandu Husada
Sofia Mubarika Haryana
Departement Of Histology And Biology Cell, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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ISOLASI DAN AMPLIFIKASI GEN PENYANDI DOMAIN C-TERMINUS Latent Membrane Protein (Imp-I ) Epstein-barr Virus(EBV) DARI PENDERITA KARSINOMA NASOFARING (KNF) Aris Haryanto; Sofia Mubarika
Jurnal Sain Veteriner Vol 18, No 2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.518

Abstract

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PREPARATION OF LYMPHOCYTE CULTURE CELL FROM PERIPHERIAL BLOOD OF NASOPHARYNGEAL CARCINOMA PATIENTS Aris Haryanto; Sofia Mubarika; Nastiti Wijayanti
Jurnal Sain Veteriner Vol 18, No 1&2 (2000)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8239

Abstract

Lymphocyte is leukocyte component that difficult to culture in vitro. Several viruses need lymphocytes as host cell in order to proliferate and growth in this media such as Epstein-Barr virus (EBV). This virus was associated with malignant disease like nasopharyngeal carcinoma (NPC). The objectives of this research are to develop and to prepare lymphocyte cell culture as material source of DNA for molecular analysis of virus. Peripheral blood was collected from NPC patients which is histopatologically and serologically positive of EBV. Lymphocytes were separated from the other blood components using ficcol-histopaque. Lymphocytes were diluted using RPMt medium then they were cultivated into 96 microwell plate with concentration of 106 cell/ml. The medium consist of 10% FBS, RPMI, Penstrep and FK 506. Culture of lymphocytes were incubated in 5% CO2 at 37°C. The lymphocyte cultures developed and grew confluendy during the first week. Only B cells whith EBV would be well establish. After 50 cell generations, lymphocytes were transformed and immortalized to be lymphoblastoid cell fine (LCL).
The Relation Between the Expression of latent Membrane Protein-1 Murtono C, Sofia Mubarika H, Soeripto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 02 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: In vitro studies reveal that LMP1 induces the expression of many factors for invasion and metastasis. Objective: The aims of this study were to investigate the role of LMP1 expression on the status of Nand M that take place either directly or sequentially through its relation with the expression IL4, IL6. IL 10. IFN-gamma. Gran2yme B and with protease MMP2. MMP9. either in the serum or in the tumour tissue. Methods: Forty one 41 EBER-RISH confirmed NPC cases with parallel serum were used for this study. Immunohistochemistry was used to study the expression of proteins in the tissue. The serum level of proteins were determined with ELISA. Statistical analysis used Lisrel 8.50. Results: No correlation between the expression of LMP1 with the status of Nand M of the tumour either directly or that sequentially took place through the expression of the cytokines and MMPs either in the tumour tissue or the serum level. The serum level of MMP9 was correlated with the serum level of IL6, the status of N. and the expression of IL 10. Status N was correlated with serum level of MMP2. Conclusion: There is a process that sequentially took place through the increase of the cytokines. MMPs, either in the tumour tissue or serum level and N. M status. LMP1 expression was not involved in the process. Key words: NPC - metastasis - MMP - cytokine
The effect of mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) leaf etanolic extract on splenic NK 1.1 cells activity Muhammad Ghufron Marsetyawan Soesatyo Sofia Mubarika Haryana , Sismindari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 03 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background : Mahkota dewa (Pheleria macrocarpa (Scheff, Boerl) is an herbal medicine which has been used since many years as traditional medicine in Indonesia against infections.Objective: The study was aimed to know the effect of mahkota dewa extract on cellular immune response, in particular NK1.1 cell activities.Methods: Forty C57BL/6 mice used in this study were divided into 8 groups. In this study, different doses of etanolic extract of mahkota dewa leaf were used i.e. 1.05 mg, 2.1 mg, and 4.2 mg/20g BW. Groups I, II, and III were treated with 1 mg, 2.1 mg, and 4.2 mg/20 g BW of the extract daily for 30 days. In group IV, V, and VI the extract was given simultaneously during 30 days, then the mice were infected with 104 cfu of Listeria monocytogenes (A TCC-191151 for stimulating their immune responses. Whereas group VII was untreated control group, and group VIII received only Listeria monocytogenes. All mice were then sacrificed 48 hours after the last treatment. Splenocyte NK1.1 cells were collected then cultured with YAC-1 (ATCC:TIB 1601 target cells for killing activity assay, expressing NKG2D, CD122 and IFNy assay. Results: The results showed that an oral administration of the extract significantly increased the killing activity of splenic NK 1.1 cell against the target, Y AC-1 cell. Moreover, the extract promoted the secretion of IFN-y from NK1.1 cells, and also induced expression of both surface molecule NKG2D and CD 122. The strongest effect stimulation was on the dosis of 2.1 mg/20 g BW.Conclusion: The extract had effect to augment splenic NK1.1 cell activities, as indicated by increasing their killing activity, expression of surface molecules and IFN-y production.Key words: pha/eria macrocarpa - splenic NK1.1 cell activity - YAC-1 cell - IFNy - NKG2D and CD122 surface molecules
The Effects of new T cell line derived lymphokines on B cell activation Sofia Mubarika Haryana Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Telah diketahui bahwa untuk aktivasi limfosit B diperlukan faktor pertumbuhan yang disebut limfokin atau sitokin. Banyak bukti menunjukkan bahwa limfosit T yang teraktivasi menghasilkan substansi yang dapat memacu proliferasi atau diferensiasi limfosit B. Dewasa ini telah dapat diidentifikasi berbagai limfokin yang disebut sebagai interleukin (IL) seperti IL-2, IL-3, IL-4, IL-5, IL-6 dan IL-7 yang ikut berperan dalam aktivasi sel B. Namun, mekanisme aktivasi sel B oleh limfokin sampai sekarang masih terus berkembang dan dipelajari. Untuk dapat lebih memahami aktivasi sel B, dalam penelitian ini telah dilakukan pembuatan suatu T cell line, dan telah dikarakterisasi, yakni menunjukkan fenotipe Thyl+, Lyt1+2+, Dalam penelitian ini berbagai aktivator poliklonal lipopolysaccharide (LPS), ConA, dextran sulfat dipakai untuk memacu T cell line menghasilkan limfokin. Aktivitas limfokin pada supernatannya kemudian dianalisis dengan menggunakan blastogenesis assay dengan 3H-thymidine dan hemolytic plaque assay.Hash penelitian menunjukkan bahwa T cell line menghasilkan faktor pertumbuhan yang memacu proliferasi sel B (B cell growth factor II = BCGF II atau IL-5) dan faktor yang memacu sel B untuk berdiferensiasi (B cell differentiating factor = BCDF atau IL- 6) dan menghasilkan igM dan IgG.Key words : blastogenesis assay -- hemolitic plaque assay -- B cell activation -- T cell line -- lymphokines
Gene and antibody molecule Sofia Mubarika Haryana Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 13, No 02 (1981)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (464.382 KB)

Abstract

Five major structural types of immunoglobulin in man can be distinguished. i. e. immunoglobulin G (abbreviated to IgG), IgM, IgA, IgD and IgE. Each type of immunoglobulin can synthesize specific antibody according to the inducing antigen. The specificity of immunoglobulin molecule is found in the antigen-binding fragment.Actually the specificity and the diversity of the immunoglobulin is controlled by the gene in the chromosome. The diversity and where the assembly of immunoglobulins is held are described.Key Words: immunoglobulin - antigen-binding fragment - central dogma - protein synthesis - germ line theory
The effect of a-terpineol on cell cycle, apoptosis and Bcl-2 family protein expression of breast cancer cell line MCF-7 Damiana Sapta Candrasari; Sofia Mubarika; Mae Sri Hartati Wahyuningsih
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 47, No 2 (2015)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (595.147 KB) | DOI: 10.19106/JMedSci004702201502

Abstract

ABSTRACT The cytotoxic activity of a-terpineol on T47D and HeLa cancer cell lines have been reported. This study was conducted to evaluate the effect of a-terpineol on cell cycle, apoptosis and Bcl-2 as well as Bax expression on MCF-7 cell line. The cytotoxic activity of a-terpineol was determined using MTT cell assay. Cell cycle and apoptosis were analysed using flowcytometry, whereas Bcl-2 and Bax expression were evaluated using immunohistochemistry. The results showed that a-terpineol had cytotoxic effect on the MCF-7 cell lines with an IC50 value of 33.0 ± 5.4 μg/mL. a-Terpineol induced cell accumulation in Sub-G1 lead to apoptosis of the MCF-7 cell. Moreover, a-terpineol inhibited Bcl-2 and induced Bax expressions. In conclusion, a-terpineol has potential anticancer activity against MCF-7 cancer cell line trough through cells cycle inhibition and apoptosis stimulation.
Long Non-Coding RNA (lncRNA) and MicroRNA ( miRNA) in Cancer Management Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (109.794 KB) | DOI: 10.19106/JMedScieSup004804201630

Abstract

AbstractThe discovery of microRNA, a small non coding RNA, has shed light to the dark matters (98%) of the genome. This finding resulted in a Nobel Prize awarded to Fire and Mello in 2006. miRNA a small non coding RNA  which played  a very important role in regulating protein expression through  3”UTR  or other binding places to mRNA target. miRNA have been considered as negative regulators of protein coding gene expression that may impact in cell differentiation, proliferation,  survival and all fundamental cellular processes, also  implicated in carcinogenesis. miRNA can be grouped into tumor suppressor miRNA (miRSuppressor) and oncogenic miRNA (OncomiR). miRSuppressor regulates protein expression through targeting oncogenic mRNA, meanwhile OncomiR target mRNA Tumor Suppressor. Evidence indicates that deregulation in genetic and epigenetic may cause overexpression of oncomiR and loss of expression of Tumor Suppressor miR.  In addition to that, in recent years, evidences showed that cell-to-cell communication conducted via exosome, which is released from every cell in physiological and pathological conditions andconsidered as fingerprints of cell and its status. This is a paramount biomarker discovery in cancer. In subsequent years, a lot of research further performed for the development of new cancer therapy. Our team GenomiR present our preliminary data on several miRNA in cancers aimed to develop minimal invasive biomarkers in cancer. Recently, the long non coding (lnc) RNA, another class of non-coding RNA have also attracted interest from many scientists in the world. lncRNA have emerged as an essential regulator in almost all aspect of biology included carcinogenesis. lncRNA considered as emerging key player in non-coding world.nCRNA (miRNA and lncRNA) in the context of cancer management will be discussed in this presentation
The Expression of hsa-miR-155-5p in Plasma Samples Of Breast Cancer Before And After Chemotherapy Meutia Srikandi Fitria; Sofia Mubarika Haryana; Sumadi Lukman Anwar; Teguh Aryandono; Dewi Sahfitri Tanjung; Aprilia Indra Kartika; Risky Oktriani; . Irianianiwati; Dwi Nur Indah Sari
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (93.072 KB) | DOI: 10.19106/JMedScieSup0048042016018

Abstract

Breast cancer has emerged as the most common cancer-related mortality among women worldwide. Therefore, early cancer detection using biomarkers such as microRNA is needed. One of microRNAs that has an important role in breast cancer development is miR-155. Hsa-miR-155-5p is an oncomir that is commonly dysregulated in breast cancer. This study aims to determine the expression of hsa-miR-155-5p in breast cancer patient’s plasma before and after chemotherapy. We collected 64 samples from breast cancer patients admitted to Dr. Sardjito Hospital in Yogyakarta. RNA from plasma was extracted using RNA Isolation Kit miRCURY-Biofluid. cDNA synthesis was performed using cDNA Synthesis kit II and quantification of miR-155-5p using ExiLent SYBR Green master mix (Exiqon). qRT-PCR results were then analyzed with Livak's method and compared (before and after chemotherapy) with t-test. Expression of miR-155-5p in the breast cancer patients’ plasma after chemotherapy was significantly increased (10.59 times) when compared to before chemotherapy (p = 0.001). We concluded that there was upregulated expression of miR-155-5p after chemotherapy than before chemotherapy. There has not been a known, relevant pathway between hsa-miR-155-5p and chemotherapy regimens nor resistance to chemotherapy. Keywords: Breast cancer, plasma, hsa-miR-155-5p, oncomiR, chemotherapy.
Over- and down-expression mir-29c and mir-21 after chemotherapy and radio-therapy in nasopharyngeal carcinomas and the down-regulating proteins encoding eipstein barr virus and c-Myc. Tirta wardana; Cita Herawati; Risky Oktriani; Sumadi Lukman Anwar; Indwiani Astuti; Teguh Aryandono; Sofia Mubarika Haryana
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016): SUPPLEMENT
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (173.652 KB) | DOI: 10.19106/JMedScieSup004804201622

Abstract

Nasopharyngeal carcinoma (NPC) is the type of cancer related to multiple risk factors, including infection by Epstein Barr Virus (EBV). Standard treatment of NPC involves radiotherapy and chemotherapy in local and advanced tumors, while metastatic cases are treated with systemic chemotherapy. However, there is limited data on the causes of tumor recurrence, resistance, and progression. Moreover, the initial symptoms of NPC were often neglected until later enlarged, thus making it difficult to manage. MicroRNA (miRNA) is short molecule with 18-24 nucleotides and functions as protein-expression regulator protein in post-transcription. This study was aimed to determine miRNA expression and its relationship with the incidence of NPC. miR-21 and miR-29c were known to be involved in the development of NPC and resistance. A total of 51 plasma samples and 17 tissue samples were collected from Dharmais Hospital. The samples were taken from 17 untreated patients, 17 treated patients, and 17 healthy participants as control. We examined miRNA, protein of protein EBV (EBNA), and c-Myc expression using immunohistochemistry and quantitative polymerase chain reaction (qPCR). Our study revealed an increased expression of miR-21 and decreased expression of miR-29c in patients with NPC. There was also a correlation between the regulation of expression of miR-21 and c-Myc in the treated group of patients, and decreased expression in patients with complete response (CR) (4.13 ± 3.65: 2.74 ± 3.23; p <0.1). The parameters tend to increase in patients with partial response (PR) (3.00 ± 5, 86 compared to 8.77 ± 8.43; p <0.5), while no significant difference in expression of miR-29c in patients with CR and PR was detected. We concluded that miRNA might be detected in the plasma of NPC patients, and miR-21 might become a useful biomarker to determine therapeutic outcome in NPC patients.Keywords: nasopharyngeal cancer; miRNA; biomarker
Co-Authors . Irianianiwati . Suharno Abdurahman Laqif Abdurahman Laqif Addin Trirahmanto Agnes Murdiat Agnes Murdiati Agus Surono Ahmad Ghozali Ahmad Ghozali Ahmad Hamim Sadewa Akbar Satria Fitriawan Akira Hosoyama Akira Hosoyama Alfasunu, Serafim Aminuyati Anwar, Sumadi Lukman Aprilia Indra Kartika Aprilia Indra Kartika Aris Haryanto Aris Haryanto Arsi Palupi Atsushi Yamazoe Atsushi Yamazoe AWM. Boersma Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hariwiyanto Bernadia Branitamahisi Bernadia Branitamahisi Bolhuis RLH Camelia Herdini Christiana Tri Nuryana Christina Hari Nawangsih Priharsanti Christina Prihharsanti Cita Herawati Daan Khambri Damiana Sapta Candrasari Danarto Danarto Danarto Danarto Danarto Danarto Demas Bayu Handika Dessy Arisanty Dewi Agustina Dewi Sahfitri Tanjung Dewi Sahfitri Tanjung Diah Rumekti Hadiati Dibyo Pramono Didik Setyo Heiyanto Didik Setyo Heriyanto Dinna Rakhmina Dwi Aris Agung Nugrahaningsih Dwi Nur Indah Sari Edy Meiyanto Eka Savitri Endang Astuti Endang Astuti Fatma Zuhrotun Nisa Fatma Zuhrotun Nisa Firly Putri Fardhila Hanafi, Arif Riswahyudi Hartopo, Anggoro B. Heru Pradjatmo Hideaki Nojiri Hideaki Nojiri Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Ganjar, Ibnu G. Ibnu Purwanto Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ifrinda Giantari Imelda, Priscillia Indwiani Astuti Indwiani Astuti Indwiani Astuti Indwiani Astuti Indwiani Astuti Iqmal Tahir Irianiwati Widodo Isnatin Miladiyah Iwan Dwiprahasto Iwan Dwiprahasto Jaap Middeldorp Jajah Fachiro JAKA WIDADA Jayusman, Achmad Mulawarman Jumina Jumina Juwita Raditya K. Nooter K. Nooter Ketut Sofjan Firdausi, Ketut Sofjan KV Rao, KV M. Munir Mae S.H. Wahyuningnsih Mae Sri Hartati Wahyuningsih Mark T Hamann, Mark T Mark T. Hamann Mary Astuti Mary Astuti Maya Esther Wullur Moningka Meutia Srikandi Fitria Mohammad Hakimi Nanda Qoriansas Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Neneng Ratnasari Nilasari, Fita Nooter K Nor Sri Inayati Nor Sri Inayati Novi Febrianti Nur Arfian Nur Arfian Nur Signa Gumilas Oktriani R Oostrum RG Perkasa, DP Pinandi Sri Pudyani Puji Lestari R.L.M. Bolhuis Rachma Greta Perdana Putri Rachma Greta Putri Raden Danarto Renovaldi, Dede Retno Arianingrum Retno Sunarminingsih Sudibyo Rina Triasih Risky Oktriani Ronny Martien S. Sismindari Sagung Rai Indrasari Salugu Masesadji Sari Eka Pratiwi Satriyo, Pamungkas Bagus Sa’adah N Shanti Listyawati SHANTI LISTYAWATI Shanti Listyawati Shinta Hartanto Siregar, Fajri M. Sismindari . Sismindari Sismindari Sismindari Sismindari Siti Boedina Kresno Siti Nur Chasanah Siti Nur Chasanah Soenarto Sastrowiyoto Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Suparwitri Stefani Candra Firmanti Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sukarti Moeljopawiro Sumadi, Firasti A.N Sumawan, Herman Susanna Hutajulu Tatsuo Takeya, Tatsuo Teguh Aryandono Tiara Puspita Agustin Tirta wardana Torizal GF Tri Wibawa Triana Hertiani Umar Anggara Jenie Umar Anggara Jenie Wardana T wardana, Tirta Widhiastuti, Stefani Santi Wirsma Arif Harahap Yanwirasti - Yohanes Widodo Wirohadidjojo Yosi B. Murti Yosi Bayu Murti Ysrafil .