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All Journal Jurnal Sain Veteriner SAINS DAN MATEMATIKA Health Science Journal of Indonesia Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Pharmaciana: Jurnal Kefarmasian Indonesian Journal of Urology agriTECH Indonesian Journal of Biotechnology INDONESIAN JOURNAL OF PHARMACY BERITA BIOLOGI Dental Journal (Majalah Kedokteran Gigi) The Indonesian Biomedical Journal Universa Medicina Medical Laboratory Technology Journal Molecular and Cellular Biomedical Sciences (MCBS) Molekul: Jurnal Ilmiah Kimia Oto Rhino Laryngologica Indonesiana INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY Journal of Herbal, Clinical and Pharmaceutical Science (HERCLIPS) Narra J Cermin Dunia Kedokteran Nutrisia Medical and Health Journal Jurnal Pandu Husada Berita Biologi
Sofia Mubarika Haryana
Departement Of Histology And Biology Cell, Faculty Of Medicine, Public Health And Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Education Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Veterinary Other

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Exosomal miRNAs as Potential Biomarkers for Preeclampsia: miR-1283 Has the Highest Expression, while miR-152-3p Has the Lowest Expression Sumawan, Herman; Giantari, Ifrinda; Mubarika, Sofia; Hadiati, Diah Rumekti; Pradjatmo, Heru
The Indonesian Biomedical Journal Vol 16, No 4 (2024)
Publisher : The Prodia Education and Research Institute (PERI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18585/inabj.v16i4.3197

Abstract

BACKGROUND: Preeclampsia management is necessary, as it is one of the leading causes of death during pregnancy. Exosomal microRNAs (miRNAs) can serve as biomarkers for early detection, diagnosis, and prognosis of preeclampsia. NanoStrings is an effective method for identifying exosomal miRNA due to their high sensitivity and ability to work with small amounts of miRNA; however, the analysis using this method for determining preeclampsia biomarker is still limited. Therefore, this study was conducted to utilize the NanoStrings method in identifying preeclampsia biomarkers related to its underlying pathophysiology.METHODS: This study involved 12 pregnant women at 20–40 weeks of gestation, including 6 preeclampsia women and 6 normotension women. The miRNAs from plasma exosomes were processed using NanoStrings method with NanoString nCounter SPRINT Profiler. Enrichment analysis of The Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathways were performed to examine the pathophysiological pathways of preeclampsia, using the DIANA–miRPath v3.0.RESULTS: Forty-eight miRNAs were downregulated and 7 were upregulated (miR-1283, miR-613, miR-520a-3p, miR-3185, miR-556-3p, miR-1973, and miR-598-3p) in women with preeclampsia. The highest expression was observed in miR-1283 (log fold-change: 3.69) and the most lowest expression was in miR-152-3p (log fold-change: 1.41). Enrichment analysis showed that the most upregulated miRNAs pathways was estrogen signaling pathway, and the most downregulated was Hippo signaling pathways.CONCLUSION: miR-1283 has the highest expression, while and miR-152-3p has the lowest expression in preeclampsia women. These miRNAs are shown to be linked to specific pathways, shedding light on the pathophysiology of preeclampsia, and may serve as promising biomarkers.KEYWORDS: exosomes, biomarker, miRNAs, pathophysiology, preeclampsia, pregnancy
EXPRESSION OF HSA-MIR-22-3P IN THE URINE OF PROSTATE CARCINOMA PATIENTS AS A NON-INVASIVE BIOMARKER Prasetyo, Angga Dwi; Danarto, R.; Haryana, Sofia Mubarika; Astuti, Indwiani
Berita Biologi Vol 24 No 3 (2025): Berita Biologi
Publisher : BRIN Publishing (Penerbit BRIN)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/berita_biologi.2025.11311

Abstract

Prostate carcinoma is one of the prostate diseases with the highest prevalence in men. Many factors cause the disease; some are androgen receptor disorder, mutation of genes, age, epigenetics, and environment. Currently, the detection of the disease is done by Prostate-Specific Antigen (PSA), Transurethral Resection of Prostate (TURP), and Digital Rectal Examination (DRE) tests; all of which are invasive to the patients. The microRNA that exists in urine exosomes can be used to detect non-invasive prostate carcinoma.  Hsa-miR-22-3p with Gleason Score. This study aimed to examine the expression potential of Hsa-miR-22-3p in urine samples of prostate carcinoma exosomes as a non-invasive biomarker to determine the correlation between the expression of Hsa-miR-22-3p and the value of the Gleason Score. This study is of cross-sectional observational analysis. Urine samples were obtained from RSUP dr. Sardjito Yogyakarta dan RSUP dr. Soeradji Tirtonegoro. The exosome isolation was then carried out, followed by RNA isolation, cDNA synthesis, and quantification using qRT-PCR. Based on the result of the study, there was a decrease in the expression of Hsa-miR-22-3p by 6.6 times in prostate carcinoma; there was a significant difference between the samples of prostate carcinoma and healthy individuals (P = 0,031), and there was a correlation between the expression level of Hsa-miR-22-3p and the value of Gleason Score. Therefore, Hsa-miR-22-3p has the potential to be used as a biomarker for prostate carcinoma patients.
Chitosan nanoparticle‐mediated delivery of anti‐miR‐203a‐3p for 4T1 triple‐negative breast cancer Temartenan, Jecklyn Shindy; Fiqri, Hairil; Satriyo, Pamungkas Bagus; Haryana, Sofia Mubarika
Indonesian Journal of Biotechnology Vol 31, No 1 (2026)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/ijbiotech.110535

Abstract

Anti‐miR molecules can suppress specific microRNA (miRNA) functions within critical signaling pathways. Chitosan acts as a delivery system for miRNAs; therefore, encapsulating miR‐203a‐3p is essential for targeted delivery and biological activity. This study investigates the impact of chitosan‐encapsulated anti‐miR‐203a‐3p nanoparticles (CS‐NPs) on the viability, proliferation, and migration of triple‐negative breast cancer (TNBC) 4T1 cells. The nanoparticles were synthe‐ sized using the ionic gelation method in a 5:1 ratio of chitosan to anti‐miR‐203a‐3p, incorporating sodium tripolyphosphate (STPP) as a crosslinker. Characterization was conducted using gel electrophoresis and particle size analysis. Cytotoxicity and cell viability were assessed through the MTT assay, while colony formation and wound healing assays evaluated cell proliferation and migration. The nanoparticles demonstrated an encapsulation efficiency of 89.47% and showed significant inhibitory effects on 4T1 cell proliferation and migration. The MTT results indicate an IC50 value of 2.454 µM, while colony formation analysis revealed that both ½ and IC50 doses significantly reduced colony numbers compared to the control. Similarly, wound healing assays showed notable inhibition of cell migration at ¼, ½, and IC50 concentrations. These findings suggest that anti‐miR‐203a‐3p‐loaded CS‐NPs may offer a promising therapeutic approach to managing aggressive breast cancer subtypes, particularly TNBC.
MiR-141-3p Relative Expression Level from FFPE Samples as Biomarker of Prostate Adenocarcinoma Carcinogenesis in Yogyakarta, Indonesia Pratiwi, Sari Eka; Wahyuningrum, Sri Nuryani; Putri, Rachmagreta Perdana; Danarto, Danarto; Heriyanto, Didik Setyo; Arfian, Nur; Haryana, Sofia Mubarika; Astuti, Indwiani
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 4 No 1 (2022): The future of diagnostic laboratory testing
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v4i1.2355

Abstract

Globally, prostate cancer (PCA) is the second leading cause of male cancer-associated mortality. Micro-RNAs (miRNAs) are small non-coding RNAs considered promising biomarkers for diagnosis, prognosis, and treatment options. A miR-141 expression is frequently dysregulated and influences the development and progression of PCA. This study aimed to identify miR-141 expression level as a marker to differentiate PCA from another prostate anomaly, especially in Yogyakarta. Formalin-fixed paraffin-embedded (FFPE) tissues for each three groups: benign prostatic hyperplasia/BPH, high-grade prostatic intraepithelial neoplasia/HGPIN, and PCA (n=7/group) were stored in a commercial clinical laboratory in Yogyakarta. The total RNA was extracted from FFPE sections using miRNeasy FFPE kit, followed by the quantification of miR-141-3p expression level by RT-PCR. The result showed that miR-141 relative expression level on PCA was higher than other groups and significantly different (P<0.05, Kruskal Wallis test). The mean of the miR-141 relative expression level of BPH, HGPIN, and PCA were 1.04±0.87, 6.44±7.8, and 7.06±8.83, respectively. The relative expression level of miR-141 can potentially be a prognostic biomarker in PCA and could differentiate aggressiveness in prostate anomaly, especially BPH, HGPIN, and PCA.
Co-Authors . Irianianiwati . Suharno Abdurahman Laqif Abdurahman Laqif Addin Trirahmanto Agnes Murdiat Agnes Murdiati Agus Surono Ahmad Ghozali Ahmad Ghozali Ahmad Hamim Sadewa Akbar Satria Fitriawan Akira Hosoyama Akira Hosoyama Alfasunu, Serafim Aminuyati Angga Dwi Prasetyo Anwar, Sumadi Lukman Aprilia Indra Kartika Aprilia Indra Kartika Aris Haryanto Aris Haryanto Arsi Palupi Atsushi Yamazoe Atsushi Yamazoe AWM. Boersma Bambang Hariwiyanto Bambang Hariwiyanto Bambang Hariwiyanto Bernadia Branitamahisi Bernadia Branitamahisi Bolhuis RLH Camelia Herdini Christiana Tri Nuryana Christina Hari Nawangsih Priharsanti Christina Prihharsanti Cita Herawati Daan Khambri Damiana Sapta Candrasari Danarto Danarto Danarto Danarto Demas Bayu Handika Dessy Arisanty Dewi Agustina Dewi Sahfitri Tanjung Dewi Sahfitri Tanjung Diah Rumekti Hadiati Dibyo Pramono Didik Setyo Heiyanto Didik Setyo Heriyanto Dinna Rakhmina Dwi Aris Agung Nugrahaningsih Dwi Nur Indah Sari Edy Meiyanto Eka Savitri Endang Astuti Endang Astuti Fatma Zuhrotun Nisa Fatma Zuhrotun Nisa Fiqri, Hairil Firly Putri Fardhila H R Danarto Hanafi, Arif Riswahyudi Hartopo, Anggoro B. Heru Pradjatmo Hideaki Nojiri Hideaki Nojiri Ibnu G Gandjar, Ibnu G Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Gandjar Ibnu G. Ganjar, Ibnu G. Ibnu Purwanto Ida Ayu Preharsini Ida Ayu Preharsini Kusuma Ifrinda Giantari Imelda, Priscillia Indwiani Astuti Indwiani Astuti Indwiani Astuti Indwiani Astuti Indwiani Astuti Iqmal Tahir Irianiwati Widodo Isnatin Miladiyah Iwan Dwiprahasto Iwan Dwiprahasto Jaap Middeldorp Jajah Fachiro JAKA WIDADA Jayusman, Achmad Mulawarman Jumina Jumina Juwita Raditya K. Nooter K. Nooter Ketut Sofjan Firdausi, Ketut Sofjan KV Rao, KV M. Munir Mae S.H. Wahyuningnsih Mae Sri Hartati Wahyuningsih Mark T Hamann, Mark T Mark T. Hamann Mary Astuti Mary Astuti Maya Esther Wullur Moningka Meutia Srikandi Fitria Mohammad Hakimi Nanda Qoriansas Nastiti Wijayanti Nastiti Wijayanti Nastiti Wijayanti Neneng Ratnasari Nilasari, Fita Nooter K Nor Sri Inayati Nor Sri Inayati Novi Febrianti Nur Arfian Nur Arfian Nur Arfian, Nur Nur Signa Gumilas Oktriani R Oostrum RG Pamungkas Bagus Satriyo Perkasa, DP Pinandi Sri Pudyani Puji Lestari Putri, Rachmagreta Perdana R. Danarto, R. R.L.M. Bolhuis Rachma Greta Perdana Putri Rachma Greta Putri Raden Danarto Renovaldi, Dede Retno Arianingrum Retno Sunarminingsih Sudibyo Rina Triasih Risky Oktriani Ronny Martien S. Sismindari Sagung Rai Indrasari Salugu Masesadji Sari Eka Pratiwi Sa’adah N Shanti Listyawati SHANTI LISTYAWATI Shanti Listyawati Shinta Hartanto Siregar, Fajri M. Sismindari . Sismindari Sismindari Sismindari Sismindari Siti Boedina Kresno Siti Nur Chasanah Siti Nur Chasanah Soenarto Sastrowiyoto Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Nuryani Wahyuningrum Sri Suparwitri Stefani Candra Firmanti Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Subagus Wahyuono Sukarti Moeljopawiro Sumadi, Firasti A.N Sumawan, Herman Susanna Hutajulu Tatsuo Takeya, Tatsuo Teguh Aryandono Temartenan, Jecklyn Shindy Tiara Puspita Agustin Tirta wardana Torizal GF Tri Wibawa Triana Hertiani Umar Anggara Jenie Umar Anggara Jenie Wardana T wardana, Tirta Widhiastuti, Stefani Santi Wirsma Arif Harahap Yanwirasti - Yohanes Widodo Wirohadidjojo Yosi B. Murti Yosi Bayu Murti Ysrafil, Ysrafil