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All Journal Biospecies Bioeducation Journal Pendidikan Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Eksakta : Berkala Ilmiah Bidang MIPA Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat SIMBIOSA MASALIQ: Jurnal Pendidikan dan Sains Jurnal Serambi Biologi
Dezi Handayani
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Optimasi Kondisi Fermentasi Cendawan Endofit Andalas (Morus Macroura Miq.) Isolat CED 3 Untuk Menghasilkan Senyawa Antibakteri Siti Nurayni; Dezi Handayani
Jurnal Serambi Biologi Vol. 6 No. 2 (2021): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Andalas plant is medicinal plants with high potential in producing antimicrobial compounds. The direct use of antibacterial compound from Andalas plants can destroy its existence. Endophytic fungi are known to be able produce the same bioactive compounds as their host. Production antibacterial compounds can be done through the fermentation. The optimum conditions fermentation influenced by factors medium, time, temperature and pH. This reseach aiming to determine the optimum time fermentation, the optimum pH fermentation medium and the optimum temperature fermentation the endophytic fungus isolate CED 3 isolated from the leaves Andalas plant (Morus macroura Miq.) in producing antibacterial compounds. Antibacterial activity test by disc diffusion method. This research is a descriptive, held on January 2021 to June 2021 in the research laboratory of the Biology Department, FMIPA UNP. The results showed the optimum fermentation time for endophytic fungi isolate CED 3 was on day 6 against S. aureus with an average inhibition zone of 9.6 mm and 9,4 against E. coli. Optimizing pH optimum medium on pH 6 against S. aureus and pH 7 against E.coli. Meanwhile, optimum temperature optimization of isolate CED 3 was at 28°C-30°C against S. aureus and E.coli optimum at 26°C.
POTENTIAL OF THERMOPHIC BACTERIAL XILANASE AS ENVIRONMENTALLY FRIENDLY FABRIC BLEACH Nur Shofiatun Nisa; IRDAWATI IRDAWATI; Dwi Hilda Putri; Dezi Handayani; Y Yusrizal
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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The main conclusion contains what is the implication to other studies or to the future brought by the study. Xylanase is an enzyme that is widely used in industry because this enzyme can hydrolyze xylan (hemicellulose) into xylo-oligosaccharides and xylose. This enzyme can be produced by thermophilic bacteria. One of the benefits of xylanase is as an environmentally friendly fabric bleaching process (biobleaching). The use of xylanase enzymes can reduce the use of toxic chemicals such as chlorine by about 20-40%. The purpose of this study was to determine the effect of temperature and pH on xylanase production activity using rice straw xylan extract as a substrate and to see the potential of MS 18 thermophilic bacterial xylanase on fabric brightness. This research is a descriptive study with temperature and pH optimization treatment using 6 treatments and 3 replications, respectively. Enzyme activity was measured using a spectrophotometer with a wavelength of 540 nm. The data obtained are displayed in the form of tables and figures. The xylanase potential on the brightness level of the fabric was seen based on the results of the kappa number. The results of this study obtained the optimum temperature of xylanase activity at a temperature of 50℃ with the highest xylanase 3.792 U/mL. while at pH the optimum pH of xylanase activity was found at pH 7 with the highest xylanase production of 4.448 U/mL. The potential of xylanase on the brightness of the fabric based on the results of the kappa number is 2.04 lower than without using xylanase, which is 6.46.
Keanekaragaman Jamur Makroskopis Di Kawasan Hutan Mangrove Karang Sungai Pisang Kecamatan Teluk Kabung Kota Padang Sumatera Barat Kurnia Putri Indrawan; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Indonesia is a mega-biodiversity country, one of the most abundant biodiversity is fungi or mushrooms. Fungi or fungi are generally classified into two types, namely microscopic fungi and macroscopic fungi. Mangrove forests are ideal habitats for fungi because of the characteristics of the area which is always moist. This study aims to determine the types of macroscopic fungi in the Karan Sungai Pisang Mangrove Forest, Teluk Kabung District, Padang City, Sumatra. This study used a random roaming method in the mangrove forest area and the technique of collecting samples was incidental. The results obtained 9 types of mushrooms, namely: Irpex lacteus, Auricularia auricular, Myxarium sp., Lentinus sajor caju, Trametes sp. 1, Trametes sp. 2, Rigidoporus sp., Ganoderma lucidum, and Daldinia concentrica. These mushrooms belong to 6 families, namely: Phanerochaetaceae, Auriculariaceae, Hyaloriaceae Poliporaceae, Ganodermaceae, and Xylariaceae.
Diversity Of Macro fungi At The Sitinjau Lauik Hill Forest Area West Sumatera zultsatunni'mah zultsatunni'mah; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Macro fungi are large fungi, they can be seen with the naked eye. The Sitinjau Lauik Hill Forest area is a suitable habitat for the growth of macro fungi. Information about the diversity species of macro-fungi and their use for the community around The Sitinjau Lauik Hill Forest area is still very minimal. Therefore, a study was conducted with the aim of making an inventory of the types of macro fungi found in The Sitinjau Lauik Hill Forest area. The method used in this research is a direct survey in the field by exploring the forest area. Identification of fungi is done by adjusting morphological characteristics with reference books and related journals. Based on the research that has been done, the macro fungi found are Cookeina sulcipes., Genoderma sp., G. boninense., G. carnosum., G. lucidium., Pycnoporus sanguineus., Trametes sp., T. vesicolor., Microporus sp., M. afinis., Polyporus sp., P. arcularius., Grifola sp., Lycoperdon bisidium., Pleurotus pulmonarius., Auricularia polytricha., A. auricula-judae., Marasmius sp., Coltricia cinamomea., Lentinus sajor-cajor. This it can be concluded that the macro fungi found in the Hill Sitinjau Lauik Forest area amounted to 20 types of species fungi originating from 2 divisio, 5 ordo, 9 family and 12 genus.
Diversity of Macro Fungi in Bung Hatta Forest Park Collection Block, Padang City, West Sumatera Ainul Mardiah; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

The collection block is one of the blocks located in the Bung Hatta Forest Park. The condition of the area of the collection block allows the fungus to grow well. Macro fungi play an important role in forest ecosystems and have the potential as food, medicine, and some are poisonous. Considering the many types of macro fungi whose number and species are not yet known, an inventory needs to be carried out. This study aims to determine the types of macro fungi found in the Bung Hatta Forest Park Collection Block, Padang City. This research is descriptive research. The sampling technique used is the direct survey method by exploring the Collection Block area. The fungi samples found were taken and then put into a sample bottle and continued with the making of a herbarium and then identified in the botanical laboratory of FMIPA UNP. Identification of macro fungi using the Collins Fungi Guide (Buczacki, 2012) and journals. The data is presented in the form of tables and figures. The results of the study found 28 species of macro fungi consisting of 2 divisions, 2 classes, 8 orders, 14 families and 19 genera. It was concluded that the most abundant types of macro fungi were dominated by the Basidiomycota division and the least by the Ascomycota division.
Keanekaragaman Jamur Makro di Kawasan Hutan Mangrove Teluk Buo Bungus Teluk Kabung Sumatera Barat Anisa Asri; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Indonesia is the center of the world's biodiversity known as a megabiodiversity country. One area that has a high biodiversity is mangrove forests. The biodiversity found in mangrove forests is fungi. The purpose of this study is to find out what types of macro mushrooms are found in the Mangrove Forest Area of Buo Bungus Teluk Kabung West Sumatra. This type of research is descriptive, the time and place of this study was conducted in August-December 2021 in the Mangrove Forest Area of Teluk Buo Bungus Teluk Kabung West Sumatra. The method used is a direct survey into the field. Based on the research that has been done, the results obtained are 11 species of macro fungi that are mostly of the Agaricomycetes class. Based on the results of the study it can be concluded that macro mushrooms obtained are Trametes sanguinea, Trametes sp 1, T. sp 2, Polyporus leptochepalus, P. sp 1, Ganoderma sp 1, G. sp 2, Daedaleopsis sp, Schizophyllum commune, Pleurotus ostreatus, dan Auricularia angiospermum. The genus most commonly found is the genus Polyporaceae as many 5 species.
Keanekaragaman Cendawan dari Cairan Ecoenzyme dengan Sumber Bahan Organik Berbagai Jenis Kulit Jeruk Ihdina Aurora Nurul Aulia; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Ecoenzyme is a liquid from the fermentation of organic matter, sugar and water or commonly known as environmentally friendly enzymes. Ecoenzyme liquid contains various secondary metabolites produced by microbes (bacteria and fungi) that exist during the fermentation process. Ecoenzymes can generally be made from organic materials such as vegetable waste and unprocessed fruit waste. One of the organic materials that is easily obtained and produces ecoenzyme with a distinctive aroma is orange peel. The purpose of this study was to determine the types of fungi from ecoenzyme liquid with organic sources of various types of orange peel. This research is a descriptive research conducted in June-December 2021 at the Research Laboratory of the Biology Department, FMIPA UNP. The ecoenzyme samples used were a mixture of several types of citrus, namely honey orange peel, Mandarin orange, Pasaman orange, Gunung Omeh orange, lime and kaffir lime. The results showed that the ecoenzyme sample found 1 fungal isolate. The results of macroscopic and microscopic observations generally obtained that the fungal isolates were round with wavy edges, slow growth, brownish white in color, the cells were flat rod-shaped and quite long, had a rough surface, and had one nucleus. The isolates obtained showed the characteristics of yeast. Therefore, it can be concluded that it was successful to isolate 1 fungal isolate from ecoenzyme liquid with organic sources of various types of orange peels.
Jenis-Jenis Cendawan dari Ampas Ecoenzyme dengan Sumber Bahan Organik Berbagai Jenis Kulit Jeruk Sukma Yuliana; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 1 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Abstract

Ecoenzyme is a solution resulting from the simple fermentation of organic waste with the addition of sugar and water using selective microorganisms such as yeast and bacteria. In general, ecoenzymes are made from vegetable and fruit waste that has not been processed. One of the best organic ingredients to use is orange peel because it is the easiest to get and produces a distinctive and sharp aroma. The purpose of this study was to determine the types of fungi from ecoenzyme dregs with organic material sources of various types of orange peel. This research is a descriptive study which was conducted in June-December 2021 at the Research Laboratory of the Biology Department, FMIPA UNP. The ecoenzyme samples used were a combination of several types of citrus such as Pasaman orange peel, Gunung Omeh orange, lime, and kaffir lime. The results showed that 1 fungal isolate was obtained from the ecoenzyme sample. The results of macroscopic and microscopic observations generally have the same characteristics as colonies, which are brownish white in color, have a rough texture, wavy edges, slow growth, flat rod-shaped cells and quite long, each having 1 cell nucleus. With these characteristics, it shows that the isolates obtained are yeasts. Therefore, it can be concluded that we have succeeded in isolating one fungal isolate from ecoenzyme dregs with organic material sources of various types of orange peel.
Uji Daya Hambat Ekstrak Daun Gambir (Uncaria gambir Roxb.) Terhadap Pertumbuhan Bakteri Staphylococcus aureus Putri Rahma Padilla; Mades Fifendy; Irdawati; Dezi Handayani
Jurnal Serambi Biologi Vol. 7 No. 3 (2022): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Staphylococcus aureus merupakan salah satu mikroflora normal yang berada dalam mulut, apabila dipengaruhi oleh faktor predisposisi seperti perubahan kuantitas mikroorganisme menjadi tidak seimbang akan menimbulkan infeksi. Beberapa penyakit dalam rongga mulut dan sekitarnya yang dapat disebabkan oleh Staphylococcus aureus yaitu abses, ginggivitis, angular cheilitis, parotitis, staphyloccal mucositis dan denture stomatitis. Untuk mengatasi infeksi Staphylococcus aureus dapat digunakan suatu alternatif, salah satunya mencari senyawa-senyawa yang berpotensi sebagai antibiotik dari tumbuhan yang memiliki khasiat obat. Pengobatan untuk infeksi Staphylococcus aureus adalah dengan pemberian antibiotik yang dapat mengahambat pertumbuhan atau mematikan Staphylococcus aureus yang menginfeksi. Salah satu tanaman yang dapat dimafaatkan sebagai obat tradisional sebagai antibiotik adalah daun gambir (Uncaria gambir). skrining fitokimia dari ekstrak daun gambir menunjukan terdapatnya kandungan golongan senyawa kimia di dalam simplisia dan ekstrak etanol gambir yaitu alkaloid, flavonoid, saponin, dan tannin yang mempunyai sfektivitas sebagai antioksidan. Penelitian ini merupakan penelitian eksperimen, menggunakan metode RAL yang terdiri dari 6 perlakuan dan 3 pengulangan dengan pemberian ekstrak daun gambir konsentrasi 10%, 20%, 30%, 40%, 50% serta kontrol positif (Amoxicilin 10%). Hasil penelitian menunjukkan bahwa ekstrak daun gambir mampu menghambat pertumbuhan bakteri Staphylococcus aureus. Konsentrasi optimum ekstrak daun gambir dalam menghambat pertumbuhan bakteri Staphylococcus aureus pada konsentrasi 50% sebesar 21,6 mm.
Formulation of Pseudomonad Fluorescent as Plant Growth Promoting Rhizobacteria Haqil Triyatdipa; Linda Advinda; Azwir Anhar; Dezi Handayani; Irdawati
Jurnal Serambi Biologi Vol. 8 No. 1 (2023): Jurnal Serambi Biologi
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Universitas Negeri Padang

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Plant growth promoting rhizobacteria, known as Plant Growth Promoting Rhizobacteria (PGPR), are bacteria found on the surface of plant roots and are beneficial microorganisms. Fluorescent pseudomonads are a group of bacteria belonging to PGPR and have the ability to dissolve phosphates, produce nitrogen, phosphorus, siderophores, and Indole Acetic Acid (IAA) to stimulate plant growth. PGPR must be immobilized in a certain carrier in the form of a formulation so that it is easy to use, store, commercialize, and apply to the field. Due to the increasingly expensive instant media, the development of media formulations continues to be carried out which can be organic or inorganic.
Co-Authors Achyar, Afifatul Ainul Mardiah Alfatimah Azzahra, Balkis AMERIA YUNITA Anisa Asri Anizam Zein Arjulis, Wanda Armen Armen Atifah, Yusni Az-Zahra, Fauziah Azwir Anhar Celsi Ananda Claudia Ayesha Dalilah Linda Sari Lubis, Enny Della Lestari des Dhea Delara Iryanda Dilla, Aprina Dina Sukma Dini Hari Marta Dwi Hilda Putri Eka Vidya Putra Elsa Sri Handayani Elsa Yuniarti Fahra, Fahra Fevria, Resti Finti Nabila, Anggie Fransisco, Sandi Fronica, Imelda Ghean Azrin Mirza Gustina Indriati Haqil Triyatdipa Harlin, Farah Ibtisamah Heffi Alberida Helendra . Ihdina Aurora Nurul Aulia Intan Febriani IRDAWATI Irdawati Irdawati Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Kurnia Putri Indrawan Larashinda, Mentari Linda Advinda Linda Wati Mades Fifendy Meriza Fatma Miftahussa'adiah Miftahussa'adiah Moralita Chatri Mukhlis Mukhlis Nabilah, Rezi Nia Ramadhanti Novia Daniati Novita Novenra Nur Shofiatun Nisa Nurul Rahmi Oktavianes Oktavianes Putri Rachma Auliya Putri Rahma Padilla Putri, Irma Leilani Eka Putri, Ulfa Dwi Rafiqi, Aufa Rahmawati Darussyamsu Rahmi Holinesti Rani Zahara Rara Via Jonet Relsas Yogica, Relsas Resti Yulia Ristiono Ristiono Roza Yolanda Rusendra, Nabilla Makra S. Syamsurizal Safira Nurul Fadila Samsuriani Siregar Selaras, Ganda Hijrah Silfani, Novia Silviana Okwisan Siti Nurayni Sity Sarroh Sri Suciati Ningsih Sukma Yuliana Syahrastani, Syahrastani Syiifa kamila Namidya tut anggraini Vauzia, Vauzia Violita Violita Violita Violita Windi Nopitasari Y Yusrizal YOLANDA, PUTRI Yulanda Saputri Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal zultsatunni'mah zultsatunni'mah