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All Journal Biospecies Bioeducation Journal Pendidikan Biologi Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Biologi Tropis JURNAL PENDIDIKAN TAMBUSAI Eksakta : Berkala Ilmiah Bidang MIPA Atrium Pendidikan Biologi Serambi Biologi Pelita Eksakta Abdi: Jurnal Pengabdian dan Pemberdayaan Masyarakat Sainstek : Jurnal Sains dan Teknologi Jurnal Biogenerasi Suluah Bendang: Jurnal Ilmiah Pengabdian Kepada Masyarakat SIMBIOSA Jurnal Biosense MASALIQ: Jurnal Pendidikan dan Sains Jurnal Serambi Biologi
Dezi Handayani
Universitas Negeri Padang
Religion Agriculture, Biological Sciences & Forestry Humanities Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Test of the Ability of Fluorescent Pseudomonas to Dissolve Phosphate Norman, Mayanda; Advinda, Linda; Chatri, Moralita; Handayani, Dezi
Jurnal Biologi Tropis Vol. 25 No. 4b (2025): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v25i4b.11143

Abstract

Phosphate is a vital nutrient required for plants, yet its presence in the soil is frequently limited due to being held by specific ions. Microorganisms that solubilize phosphate, particularly fluorescent Pseudomonas, are considered among the most efficient agents for phosphate solubilization. This research intends to assess the capacity of six fluorescent Pseudomonas isolates derived from the rhizosphere of different plants to solubilize phosphate, and to analyze their IKF values. The research was carried out descriptively from June to August 2025 at the Plant Physiology Laboratory, Faculty of Mathematics and Natural Sciences, Padang State University. Observations were conducted by noticing the development of clear zones and determining the IKF values. The findings indicated that every isolate, specifically PfPj1, PfS36, PfLAHP2, PfCas, PfS31, and PfKd7, was capable of solubilizing phosphate. The highest IKF value was found in isolate PfKd7 (4.91), whereas the lowest was recorded for PfS36 (4.08). Variations in IKF values indicate differences in the capacity of each isolate to release organic acids, which are involved in the phosphate solubilization process. This discovery reinforces the capability of fluorescent Pseudomonas as an eco-friendly biological agent to enhance phosphorus availability.
UJI AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT PSEUDOMONAS BERFLUORESEN TERHADAP Escherichia coli, Staphylococcus aureus DAN Candida Eka Nuraini, Fauzi; Hilda Putri, Dwi; Advinda, Linda; Irdawati; Handayani, Dezi; Mardhiyah Nazri, Laila; Wulandari, Tesya; Febrianti, Liza
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.7989

Abstract

Antimicrobial resistance is increasing every year. New and better antimicrobial sources are needed. One source of antimicrobials that can be explored is from endophytic bacteria. This study aims to test the ability of fluorescent Pseudomonas endophytic bacteria from the roots of plantain lemongrass in producing antimicrobial compounds. The type of research is descriptive. The antimicrobial activity of fluorescent Pseudomonas isolates was tested using the point inoculation method using test microbes E. coli, S. aureus and C. albicans. The clear zone was observed after 24 hours. The results showed that fluorescent Pseudomonas isolates were able to inhibit E. coli with a diameter of 3.9 mm found in isolate 51. S. aureus bacteria had an inhibition zone of 9.4 mm in isolate 89. Interesting results were shown by isolate 51 in inhibiting C. albicans with an inhibition zone diameter of 29.9 mm. These differences in response are thought to be related to variations in cell wall structure and the presence of an outer membrane in Gram-negative bacteria, which inhibits the penetration of active compounds. Overall, fluorescent Pseudomonas endophytic bacteria from the roots of the plantain have the potential to be developed as a source of new antifungal candidates against C. albicans, while their antibacterial activity still needs to be optimized through compound purification and further testing.
KEMAMPUAN PSEUDOMONAS BERFLUORESEN SEBAGAI AGEN ANTAGONIS TERHADAP Ralstonia solanaceaerum PENYEBAB LAYU TANAMAN NILAM (Pogestemon cablin Benth) Pratama Putra, Abil; Advinda, Linda; Chatri, Moralita; Handayani, Dezi
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8088

Abstract

Patchouli (Pogestemon cablin Benth) is a cultivated plant that can produce oil and generate income for farmers. However, attacks by plant pests (OPT) often pose a challenge to patchouli production in Indonesia. One organism that causes wilt disease in patchouli plants is the bacterium Ralstonia solanaceaerum. Therefore, more environmentally friendly and sustainable control methods are needed. One strategy is the use of biological agents, especially antagonistic rhizosphere bacteria such as fluorescent pseudomonas. This study was conducted from June to August 2025 at the Plant Physiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Padang State University. This study used a completely randomized design (CRD) as the experimental design. In this study, there were 6 treatments (different isolates), each repeated 3 times. The parameter observed was the formation of an inhibition zone using the disc diffusion method. The data were analyzed using analysis of variance (ANOVA). If there were significant differences, Duncan's New Multiple Range Test (DNMRT) was used at a 5% level. Based on the results and discussions, it can be concluded that all fluorescent Pseudomonas isolates tested have the ability to inhibit R. solanaceaerum, the cause of wilt disease in patchouli plants. The isolate with the best ability to inhibit the growth of R. solanaceaerum on patchouli plants was isolate Pf S36 with an inhibition zone of 20.37 mm, but there was no significant difference between the other isolates because the inhibition effectiveness was relatively uniform.
AKTIVITAS ANTIMIKROBA BAKTERI ENDOFIT (Pseudomonas Berfluoresen) DARI AKAR PISANG BUAI (Musa x paradisiaca L.) Astrid; Hilda Putri, Dwi; Handayani, Dezi
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8176

Abstract

Infectious diseases caused by pathogenic bacteria and fungi remain a global health problem, particularly due to the increasing incidence of antimicrobial resistance, highlighting the need for effective alternative antimicrobial sources. Endophytic bacteria, especially fluorescent Pseudomonas, are known to produce secondary metabolites with antibacterial and antifungal properties. This study aimed to evaluate the antimicrobial activity of endophytic fluorescent Pseudomonas isolated from the roots of banana buai (Musa × paradisiaca L.) and to identify isolates with the highest activity. This experimental study employed a Completely Randomized Design (CRD) using 44 isolates with three replications. Antimicrobial activity was tested in vitro using the spot inoculation method against Escherichia coli, Staphylococcus aureus, and Candida albicans. The observed parameter was the diameter of the inhibition zone, which was analyzed using analysis of variance (ANOVA) followed by Duncan’s Multiple Range Test. The results showed that antimicrobial activity varied among isolates and was specific to the test microorganisms. Isolate PFPB 32 exhibited the highest activity against E. coli and S. aureus, while isolate PFPB 300 showed the strongest antifungal activity against C. albicans. These findings indicate that endophytic fluorescent Pseudomonas from banana buai roots have potential as a source of antimicrobial agents, particularly antifungal agents against Candida albicans
ANALISIS KONTAMINASI JAMUR PADA KUNYIT GILING CURAH DI PASAR LUBUK BUAYA KOTA PADANG Azzahra, Zulia Putri; Handayani, Dezi; Chatri, Moralita; Irdawati
Jurnal Biogenerasi Vol. 11 No. 1 (2026): Volume 11, no 1, 2026, (Januari - Maret 2026)
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/biogenerasi.v11i1.8185

Abstract

Keamanan pangan sangat penting untuk melindungi kesehatan masyarakat, khususnya untuk produk pangan yang rentan terhadap kontaminasi mikroba. Kunyit bubuk curah yang dijual di pasar tradisional berisiko terkontaminasi jamur karena praktik penanganan yang tidak higienis, yang dapat mengurangi kualitas produk dan menimbulkan bahaya kesehatan. Penelitian ini bertujuan untuk mengetahui tingkat kontaminasi jamur pada kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, dan untuk mengidentifikasi karakteristik makroskopis jamur yang tumbuh. Penelitian ini dilakukan dari Oktober 2025 hingga Januari 2026 di Laboratorium Mikrobiologi, Departemen Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Negeri Padang. Desain penelitian deskriptif digunakan dengan metode pengambilan sampel purposif. Sampel kunyit bubuk curah dipilih dan dikumpulkan dari lima pedagang dengan tingkat kebersihan yang berbeda. Kontaminasi jamur dinilai menggunakan metode penghitungan kapang dan ragi (MYC) dengan teknik spread plate pada media Potato Dextrose Agar (PDA), diikuti dengan pengamatan makroskopis morfologi koloni jamur. Hasil menunjukkan bahwa semua sampel kunyit bubuk curah terkontaminasi jamur, dengan tingkat kontaminasi berkisar antara 2,67 × 10⁵ hingga 2,9 × 10⁶ CFU/g. Semua sampel melebihi batas maksimum yang diizinkan untuk kontaminasi mikroba sebagaimana ditetapkan oleh Badan Pengawas Obat dan Makanan Indonesia (BPOM). Jamur kontaminan yang diidentifikasi meliputi Aspergillus flavus, A. niger, Penicillium sp., dan ragi. Oleh karena itu, dapat disimpulkan bahwa kunyit bubuk curah yang dijual di Pasar Lubuk Buaya, Kota Padang, tidak memenuhi standar keamanan pangan mikrobiologis.
UJI ANTAGONIS BAKTERI ENDOFIT Pseudomonas BERFLUORESEN TERHADAP RALSTONIA SOLANACEARUM PENYEBAB LAYU TANAMAN TOMAT DAN NILAM Nazri, Laila Mardhiyah; Putri, Dwi Hilda; Advinda, Linda; Nuraini, Fauzi Eka; Handayani, Dezi; Chatri, Moralita
JURNAL BIOSENSE Vol 9 No 1 (2026): Edisi Januari 2026
Publisher : Program Studi Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas PGRI Banyuwangi, Jalan Ikan Tongkol No 01, Telp (0333) 421593, 428592 Banyuwangi 68416

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36526/biosense.v9i1.6876

Abstract

Penyakit layu bakteri yang disebabkan oleh Ralstonia solanacearum merupakan salah satu kendala utama dalam budidaya tomat dan nilam karena mampu menurunkan produktivitas secara signifikan. Penggunaan agen hayati seperti pseudomonas berfluoresen menjadi alternatif pengendalian yang lebih aman dan ramah lingkungan. Penelitian ini dilakukan untuk mengetahui kemampuan antagonis tujuh isolat bakteri endofit pseudomonas berfluoresen yang berasal dari akar pisang buai dalam menghambat pertumbuhan R. solanacearum yang menginfeksi tomat dan nilam. Penelitian memakai pendekatan deskriptif-kuantitatif dan dilaksanakan di Laboratorium Biologi FMIPA UNP pada Agustus–November 2025. Isolat pseudomonas berfluoresen dan R. solanacearum diremajakan pada media selektif, kemudian diuji antagonismenya menggunakan metode swab pada medium MHA. Suspensi patogen distandarkan pada McFarland 0,5, sedangkan isolat antagonis diinokulasikan secara titik. Zona hambat yang terbentuk setelah inkubasi 24 jam diukur menggunakan jangka sorong dan dianalisis secara deskriptif. Hasil penelitian menunjukkan adanya variasi kemampuan penghambatan antar isolat. Pada patogen tomat, isolat PfB305, PfB309, PfB311, dan PfB322 menghasilkan zona hambat yang relatif besar, sedangkan PfB32, PfB51, dan PfB52 tidak menunjukkan aktivitas antagonis. Pada patogen nilam, tiga isolat yang sebelumnya tidak aktif pada patogen tomat justru menghasilkan zona hambat, sementara empat isolat lainnya tidak membentuk zona hambat. Penelitian ini menunjukkan bahwa efektivitas pseudomonas berfluoresen dipengaruhi oleh spesifisitas isolat terhadap patogen serta potensi produksi metabolit antibakteri
Improving Early Awareness of Body Organ Health in Students of State Elementary School 01 Pasar Laban Through Interactive Device-Based Education Fadilaturahmah, Fadilaturahmah; Fuadiyah, Sa’diatul; Qalbina, Putri; Anggita, Kheniva Diah; Atifah, Yusni; Marten, Threo Wanda; Achyar, Afifatul; Putri, Dwi Hilda; Handayani, Dezi; Yannita, Defni
Pelita Eksakta Vol 9 No 1 (2026): Pelita Eksakta, Vol. 9, No. 1
Publisher : Fakultas MIPA Universitas Negeri Padang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/pelitaeksakta/vol9-iss1/304

Abstract

The health of elementary school-aged children is a crucial factor that needs attention. However, the low awareness of students, especially at SDN 01 Pasar Laban, regarding the importance of maintaining healthy body organs is a problem that needs to be addressed immediately. One of the causes is the limited interactive learning methods in schools, especially regarding body organ health. Therefore, an innovative approach is needed in health education for students, namely a learning method based on interactive teaching aids "Human Body Anatomy Model". Community service activities were carried out on August 6, 2025. Material on body organ health education was delivered using an interactive lecture method followed by game-based learning using interactive teaching aids. This activity increased students' knowledge by 11.11% regarding the introduction of the structure and function of body organs and the effects of unhealthy snack consumption behavior.
Co-Authors Achyar, Afifatul Ainul Mardiah Alfatimah Azzahra, Balkis AMERIA YUNITA Anisa Asri Anizam Zein Arjulis, Wanda Armen Armen Astrid Atifah, Yusni Az-Zahra, Fauziah Azwir Anhar Azzahra, Zulia Putri Celsi Ananda Claudia Ayesha Dalilah Linda Sari Lubis, Enny Della Lestari des Dhea Delara Iryanda Dilla, Aprina Dina Sukma Dini Hari Marta Dwi Hilda Putri Eka Nuraini, Fauzi Eka Vidya Putra Elsa Sri Handayani Elsa Yuniarti Fadilaturahmah Fadilaturahmah Fahra, Fahra Febrianti, Liza Fevria, Resti Finti Nabila, Anggie Fransisco, Sandi Fronica, Imelda Fuadiyah, Sa’diatul Ghean Azrin Mirza Gustina Indriati Haqil Triyatdipa Harlin, Farah Ibtisamah Heffi Alberida Helendra . Ihdina Aurora Nurul Aulia Intan Febriani IRDAWATI Irdawati Irdawati Irma Lailani Eka Putri Irma Leilani Eka Putri Irma Leilani Eka Putri Kheniva Diah Anggita Kurnia Putri Indrawan Larashinda, Mentari Linda Advinda Linda Wati Mades Fifendy Mardhiyah Nazri, Laila Marten, Threo Wanda Meriza Fatma Miftahussa'adiah Miftahussa'adiah Moralita Chatri Mukhlis Mukhlis Nabilah, Rezi Nazri, Laila Mardhiyah Nia Ramadhanti Norman, Mayanda Novia Daniati Novita Novenra Nur Shofiatun Nisa Nuraini, Fauzi Eka Nurul Rahmi Oktavianes Oktavianes Pratama Putra, Abil Putri Qalbina Putri Rachma Auliya Putri Rahma Padilla Putri, Irma Leilani Eka Putri, Ulfa Dwi Rafiqi, Aufa Rahmawati Darussyamsu Rahmi Holinesti Rani Zahara Rara Via Jonet Relsas Yogica, Relsas Resti Yulia Ristiono Ristiono Roza Yolanda Rusendra, Nabilla Makra S. Syamsurizal Safira Nurul Fadila Samsuriani Siregar Selaras, Ganda Hijrah Silfani, Novia Silviana Okwisan Siti Nurayni Sity Sarroh Sri Suciati Ningsih Sukma Yuliana Syahrastani, Syahrastani Syiifa kamila Namidya tut anggraini Vauzia, Vauzia Violita Violita Violita Violita Windi Nopitasari Wulandari, Tesya Yannita, Defni YOLANDA, PUTRI Yulanda Saputri Yuni Ahda Yusrizal Yusrizal Yusrizal Yusrizal zultsatunni'mah zultsatunni'mah