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All Journal Jurnal Sain Veteriner Jurnal Veteriner Majalah Kedokteran Gigi Indonesia Indonesian Journal of Biotechnology Journal of Tropical Biodiversity and Biotechnology Jurnal Medik Veteriner Bioscientia Medicina : Journal of Biomedicine and Translational Research JURNAL KAJIAN VETERINER IGKOJEI: Jurnal Pengabdian Masyarakat Bioscientia Medicina : Journal of Biomedicine and Translational Research Livestock and Animal Research Jurnal Kedokteran Hewan

Tri Wahyu Pangestiningsih

Departemen Anatomi, Fakultas Kedokteran Hewan Universitas Gadjah Mada Jl. Fauna No. 2 Karangmalang, Yogyakarata

Author-ID : 2761427

Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Dentistry Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary

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A Study of the Fruit Bat (Rousettus sp) Brain Anatomy as Natural Reservoir Wild Animal for the Rabies Virus Karina Mayang Sari; Siti Khomariyah; Nur Arofah; Sabrina Wahyu Wardhani; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rousettus sp. (Fruit bat) is one type of fruit bats in Indonesia and act as a natural reservoir of rabies. Rabies is caused by a virus from genus Lyssavirus, family Rhabdoviridae, which attack central nervous system (CNS).The brain is an organ that is sensitive to rabies infection. The purpose of this study was to determine the anatomical structure of the fruit bat brain macroscopically. Five fruit bat were used in this study, they were anaesthetized using ketamine and xylazin. Animals were perfused using physiological saline and 10% buffered formalin. Brains were taken using tweezers after all the bones of the skull were separated. Analysis of macroscopic brain was done descriptively. The results showed that the fruit bat brain were generally divided into cerebrum, cerebellum and brain stem. Gyrus, sulcus and the paraflokulus lobes of the fruit bat brain were less developed than that of the dogs brain.

THE CHANGES OF THE TESTOSTERONE HORMONE LEVEL DURING PREPUBERTAL PERIODE IN INDUCED HYPOTHYROIDISM RATS Teguh Budipitojo; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8619

The one the influence factors to the normal functions and testes growth is thyroid gland status. The knowledge about the influences of euthyroid, and hypenhyroid to thetesies growth will help to give more understanding of the normal lestes growth. The new born male rats were used todetect the influence of hypothyroidism to the testosterone level during prepubertal periode. Twenty primigravida pregnant rats (Rattus norvegicus albinus) were used. After birth, the male pups were divided inio controls and treatments group. Mothers of the treatment group were given 0,1% 6-n-prapyl-2-thiauracil (PTU), via drinking water, ad libitum, after birth until on day 8. On dary 27, 30, 37, 44, and 51, five control pups and 5 treatment pups were sacrificed. The blood from each pup were collected to detec serum testosteron hormone level using immunoradLometric assay (IRMA) method. The research showed that serum testosteron level ofthe; treated group increased on day 30 (P<0,05) and day 37(P<0,05). Theresultof This research showed that PTU induced hypothyroidism produced increasing serum testosterone level.

EFFECTS OF RIBOFLAV1N SUPPLEMENTATION ON THE TERATOGENIC EFFECTS OF SODIUM BORATE IN WHITE RATS Tri Wahyu Pangestiningsih; Sumiyati Sunaryo
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Na-borate gave teratogenic effects both to rats and chickens. In chickens the fetal malformations caused by borate treatments were similar to those due to riboflavin deficiency. It was proposed that in embryonated eggs borate built a bond with riboflavin, so that riboflavin could not junction properly. This present investigation was conducted to assess the possibility of riboflavin in prevent the teratogenic effects of Na-borate in rats.Nineteen primigravida Wistar rats were allocated randomly to one of treatment groups, i. e, 9 rats were orally treated with 180 mg/200g BW/day, No-borate the other 10 rats beside treated with Na-borate, they were also treated orally with 2.5 mg/rat/day riboflavin. The treatments were done during the pregnancy on day 6 to 15. On day 20 the pregnant does were caesarean sectioned under ether anaesthesia. After all fetal malformations were noted, then all fetuses were placed in alcohol 70%, prepared for alizarin method for microscopic skeleton inspection. Chi square analysis of the gross inspection malformations, i e. the resorbed vs. the unresorbed, the alive vs. the dead, the haemorrhage vs. the non Hemorrhage fetuses of the two treatments showed unsignificant difference. Riboflavin supplementation increased singnificantly the number of fetuses with omphalocel. T-test analysis indicated that riboflavinsupplementation highly significantly caused the fore and hind paw, costal, sternal defects became more serious, if also significantly reduced the weight and the length of the fetuses. This investigation revealed that riboflavin supplementation failed to prevent, but even caused the teratogenic effects of the Na-borate to rats became more serious.

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani dengan Metode Imunohistokimia Avidin-Biotin-Peroxidase Complex Teguh Budipitojo; Ariana Ariana; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose Transporter 4 (GLUT 4) merupakan protein transporter glukosa yang diatur oleh insulin, ditemukan terutama di jaringan adiposa dan otot lurik (baik otot skelet maupun otot jantung). Ayam kedu cemani merupakan ayam endemik Indonesia yang terdapat di wilayah Kedu, Temanggung, Jawa Tengah. Penelitian ini diperlukan untuk melengkapi dokumentasi data anatomi mikroskopik kekayaan hayati asli Indonesia. Penelitian ini bertujuan untuk mengklarifikasi distribusi GLUT 4 dalam serabut otot skelet ayam kedu dengan metode imunohistokimia avidin-biotin-peroxidase complex (ABC). Penelitian dilakukan dengan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani. Hasil penelitian menunjukkan bahwa immunoreaktivitas GLUT 4 terutama terdeteksi di sarkolema dan komponen miofibril pada otot pectorales mayor, biceps brachii, maupun biceps femoris. Intensitas imunorekatifitasnya meningkat dari intensitas lemah pada otot pectorales mayor, menjadi intensitas sedang pada otot biceps brachii, dan kemudian intensitas kuat pada otot biceps femoris. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani lainnya, untuk mendokumentasi data anatomis kekayaan hayati asli Indonesia.

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani Teguh Budipitojo; Ariana -; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic documentation of Indonesia’s native biodiversity.

Distribusi neuron serotonergik pada hipokampus Lasiwen (Myotis sp) sebagai satwa liar yang berpotensi menjadi reservoir virus rabies Tri Wahyu Pangestiningsih; Ariana Ariana; Irma Padeta; Arvendi Rahma Jadi; Woro Danur Wendo
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Myotis sp is an Indonesian microchiropteran, insectivore bat that potential to be a reservoir for rabies virus. Rabies is fatal viral encephalitis and bat could act as wildlife potential reservoirs for the rabies virus and can transmit the disease to humans as the zoonotic disease. Hippocampus is one of diagnostic tissue for rabies disease and serotonergic neuron could involved in pathogenesis of rabies disease. The aim of the study is to investigate the distribution of serotonergic neurons in Myotis sp hippocampus. Five Myotis sp. were captured from wild population in Central Java, and were humanly anesthetized using ketamine and xylazin. The animals were perfused intracardially using NaCl 0.9% as the pre-rinse followed by 10% formaldehyde to fix it. The cerebrums were collected and processing the for paraffin embedding. Cerebrums were sectioning in saggital sections, 12 µm thickness serially with 120 µm intervals. The tissues were staining immunohistochemistry using antibody to serotonin (1/300; Bioss, Cat. No: bs-1126R) 2 night incubation in 4 oC temperature. The solution for blocking background, secondary antibody, avidin-biotin-peroxidae complex and chromogen using kit Starr Trek Universal HRP Detection System (Biocare Medical, Cat No: STUHRP700) and were analyzed descriptivelly. The results show that serotonergic neuros were distributed in the all area of the of Myotis sp hippocampus. In dentate gyrus neuron serotonergic (Sert-IR)s are round in shape and mostly distributed in the middle layer, few in the superficially also deeper layers. In the hippocampus , the Sert-IR neurons are pyramidal in shape and distributed in the CA1, Ca2 and CA3 areas. In subiculum, the Sert-IR neurons are pyramidal in shape, more wider distributed than in the CA1 with no differences between outer layer and deeper layer. The conclutions of this research are the serotonergic neurons are distributed in the all area of hippocampus

Kandungan L-3, 4-dihydroxyphenylalanine Suatu Bahan Neuroprotektif pada Biji Koro Benguk (Mucuna pruriens) Segar, Rebus, dan Tempe (L-3,4-DIHYDROXYPHENYLALANINE CONTENT AS A NEUROPROTECTIVE MATERIAL ON FRESH, COOKED AND FERMENTED OF KORO BENGUK (MUCUNA PR Tri Wahyu Pangestiningsih; Trini Susmiati; Hery Wijayanto
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Indonesia is rich in flora potentially used for herbal medication. One of the potential herbal is koro benguk (Mucuna pruriens) beans, where in Central Java and Yogyakarta is proccessed into tempe (fermented mucuna beans) for daily human consumption. Koro benguk has high level of L-3,4-dihydroxyphenylalanine (L-DOPA) which has a potential neuroprotective effect on Parkinson’s disease. The aim of this study was to investigate the L-DOPA content in fresh beans, cooked and fermented of koro benguk beans. The investigation were done in fresh mucuna beans, white color (BR D) and black color (BR A) beans originated from Wonogiri, Central Java, and fresh, white color (KP C), cooked, and fermented beans collected from Kulon Progo, Yogyakarta. The samples were extracted using ethanol and n-propanol solutions and were analyzed using high-performance liquid chromatography (HPLC) technique. The results show that the highest L-DOPA level (8,56%) was found in fresh white koro benguk beans from Wonogiri extracted using ethanol. The lowest L-DOPA level (0,016%) was found in fermented beans that extracted using n-propanol. Extraction using ethanol yield a higher L-DOPA level as compared to that of using n-propanol. In brief, all of the samples starting from fresh bean, cooked, and fermented koro benguk beans contain L-DOPA, with highest L-DOPA level was found in the white fresh koro benguk beans, from Wonogiri, Central Java. The lowest ingredient L-DOPA level was found in the fermented beans from Kulon Progo, Yogyakarta. ABSTRAK Indonesia sangat kaya dengan keanekaragaman flora yang potensial untuk terapi herbal, salah satunya tanaman koro benguk (Mucuna pruriens) yang bijinya bisa diolah menjadi tempe sebagai konsumsi harian masyarakat di sekitar Yogyakarta dan Jawa Tengah. Biji koro benguk diketahui mengandung L-3,4-dihydroxyphenylalanine (L-DOPA) tinggi dan berpotensi menjadi agen neuroprotektor pada penyakit Parkinson. Penelitian ini bertujuan untuk menguji kandungan L-DOPA mulai dari biji koro benguk segar, rebus, dan bahan olahannya yaitu tempe benguk. Uji dilakukan pada biji koro benguk mentah kulit berwarna putih (BR D) dan hitam (BR A) asal Wonogiri, Jawa Tengah, serta biji koro benguk mentah kulit berwarna putih (KP C), biji koro benguk yang sudah direbus dua kali, dan tempe benguk asal Kulon Progo, Yogyakarta. Sampel diekstraksi menggunakan pelarut etanol dan n-propanol, kemudian dianalisis dengan teknik high-performance liquid chromatography (HPLC) untuk melihat kadar kandungan L-DOPA-nya. Hasil penelitian menunjukkan, kadar L-DOPA tertinggi (8,56%) ditemukan pada biji koro benguk mentah dengan warna kulit putih asal Wonogiri yang diekstraksi menggunakan pelarut etanol, sedangkan kadar L-DOPA terendah (0,016%) ditemukan pada sediaan tempe yang diekstraksi dengan n-propanol asal Kulon Progo. Secara umum, ekstraksi menggunakan pelarut etanol memberikan hasil kadar L-DOPA yang lebih tinggi dibandingkan dengan pelarut n-propanol. Semua ekstraksi sampel, mulai biji koro benguk segar, rebus sampai bentuk tempenya mengandung L-DOPA, dengan kadar tertinggi terdapat pada biji koro benguk segar berkulit putih asal Wonogiri, Jawa Tengah yang diekstraksi menggunakan etanol, sedangkan kadar terendah dijumpai pada tempe benguk dari Kulon Progo, Yogyakarta yang diekstraksi menggunakan n-propanol.

Kepadatan Sel Hipokampus Insulin Imunoreaktif pada Formasi Hipokampus Mencit yang Diinduksi Berulang dengan Streptozotosin (THE DENSITY OF HIPPOCAMPUS INSULIN IMMUNOREACTIVE CELLS IN HIPPOCAMPUS FORMATION OF REPEAT STREPTOZOSIN INDUCED MICE) Erwin .; Tri Wahyu Pangestiningsih; Sitarina Widyarini
Jurnal Veteriner Vol 14 No 2 (2013)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.857 KB)

The presence of insulin in the hippocampus may indicate its involvement in brain cognitive function,such as learning and memory phenomena. The purpose of this study was to find out the density ofhippocampus insulin immunoreactive cells in hippocampus formation in Balb-C mice which treated withstreptozosin repeated as the animal model of diabetes mellitus. Thirty male mice Balb-C strain, aged 12-14 weeks, weight 30-40 g, divided into 2 treatment groups, each group consisted of 15 individuals. GroupI (KI) was treated with sodium citrate buffer, while group II (K2) was treated with streptozotosin at dose0,5 ml of 40 mg/kg bw in 50 mM sodium citrate buffer pH 4.5 in intra-peritoneal of for five consecutive days.Every two animals from each group euthanasia and necropsied on day 7, 14, 21 and 28 respectively afterthe administration of treatment. Subsequently, the brain tissues were collected and fixatived in NBF10%. Brain sampel were the processed immunohistochemically using anti-insulin mouse antibody. Thedensity of hippocampus insulin immunoreactive cells in hippocampus formation in group 1 were highercompared to group 2. This comparasion as well as the time of observation and interaction between groupand time showed significant differences (p<0.05). it can be concluded that low-dose induction of repeatedstreptozotosin may cause a decrease in density of hippocampus insulin imunoreaktif cell.

Apoptosis Neuron Nukleus Merah pada Tikus Model Penyakit Parkinson Akibat Induksi Parakuat Diklorida Ayu Miftahul Khasanah; Niswah Nurul Fahma; Tri Wahyu Pangestiningsih
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Red nucleus plays a role in motoric control, so that disturbanced of neurons in this nucleus could be affected on motor impairment. Previous study reported that paraquat (PQ) could induced Parkinson disease, a neurodegeneratif disease that affected subtantia nigra pars compactca and clinically characterized by motoric disorders. Apoptosis is a programed cell death that physiologically normal for the body, but in large quantities will cause progresive cell death. This study aimed to observed apoptosis in rats RN neurons following by PQ injection. Ten male Wistar rats with the aged of 3 month, devided into two groups of five. Control group was given aquadest and treatment group was given PQ, injected intraperitoneally, dosage 7 mg/kg BW, twice a week for three weeks. At day 24th all rats were anesthetized using ketamin dosage 40 mg/kg BW and xylazine dosage 5 mg/kg BW, perfused intracardially using 4% paraformaldehyde and mesencephalons were collected. Mesencephalons were processed for histological preparations using the paraffin method and cut stereologically in coronal section, in 4 ?m thickness. Immunohistochemistry staining was done using caspase 3 antibody as a marker of apoptosis. Data were analyzed descriptively and quantitatively. The results in both groups showed RN neurons were large size and stelat or fusiform in shape. The percentage of apoptotic neurons in the RN of treatment group was significantly increased (80,4%±13,8%) compared to the control group (26,6%±18,32%) (P<0.01). In conclusion, following PQ exposurethere is a significant increased of apoptosis neurons number in the RN of rat model of Parkinson disease.

The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model Budi Susilo; Cahya Yustisia Hasan; Bambang Dwi Raharjo; Tri Wahyu Pangestiningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i8.338

Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14.

Co-Authors . Ariana . Muttaqien Agnya Sinung Suminar Agus Purnomo Amelia Hana Amelia Hanna Amrullah Anindito Anak Agung Gede Sugianthara Aqiilah, Khairunnisa Ariana Ariana - Ariana Ariana Ariana Ariana Ariana Ariana Arvendi Rachma Jadi Arvendi Rahma Jadi Asyhari . Ayu Miftahul Khasanah Bambang Dwi Raharjo Bambang Dwirahardjo Bambang Sugito Bambang Sutrisno Budi Susilo Budiariati, Vista Cahya Yustisia Hasan, Cahya Yustisia Citra Ayu Pramesti Claude Mona Airin ClaudeM Mona Airin Daisynta Prima Aninditya Dewi Kania Musana Dewi Kania Musana Diah Tri Widayati Dondin Sajuthi Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Dwi Liliek Kusindarta Erdiansyah Rahmi Erni Sulistiawati Erwin . Erwin E Etriwati E Filphin Adolfin Amalo Gerarda Gita Puspitandaru Heni Pujiastuti Hery Widijanto Hery Wijayanto Hevi Wihadmadyatami Hevi Wihadmadyatami Iffah Sofana Intan Maharani Irene Linda Megawati Saputra Irma Padeta Jaswadi J Karina Mayang Sari Koeswinarning Sigit Koko Kurniawan Kustiati, Ulayatul Nemay Anggadewi Ndaong Nemay Anggadewi Ndaong, Nemay Anggadewi Niswah Nurul Fahma Nur Arofah Nuraini Rahmawati Nurhidayat . Nusaibah Nuraniyati Pradityo Yoga Wibowo PUDJI ASTUTI Rina Pratiwi Rini Widayanti Roza Azizah Primatika Sabrina Wahyu Wardhani Saragih, Golda Rani Sari, Yuslikha Khaniif Anggita Sitarina Widyarini Siti Khomariyah Soehartini Jatman Sumiyati Sunaryo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Teguh Budipitojo Trini Susmiati Victor Lenda Vidya Irawan Vidya Irawan, Vidya Woro Danur Wendo Woro Danur Wendo Yosephine Nicolory Paula Yuda Heru Fibrianto Yulfia N Selan Yulfia Nelymalik Selan Yulfia Nelymalik Selan, Yulfia Nelymalik

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