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A Study of the Fruit Bat (Rousettus sp) Brain Anatomy as Natural Reservoir Wild Animal for the Rabies Virus Karina Mayang Sari; Siti Khomariyah; Nur Arofah; Sabrina Wahyu Wardhani; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 32, No 2 (2014): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Rousettus sp. (Fruit bat) is one type of fruit bats in Indonesia and act as a natural reservoir of rabies. Rabies is caused by a virus from genus Lyssavirus, family Rhabdoviridae, which attack central nervous system (CNS).The brain is an organ that is sensitive to rabies infection. The purpose of this study was to determine the anatomical structure of the fruit bat brain macroscopically. Five fruit bat were used in this study, they were anaesthetized using ketamine and xylazin. Animals were perfused using physiological saline and 10% buffered formalin. Brains were taken using tweezers after all the bones of the skull were separated. Analysis of macroscopic brain was done descriptively. The results showed that the fruit bat brain were generally divided into cerebrum, cerebellum and brain stem. Gyrus, sulcus and the paraflokulus lobes of the fruit bat brain were less developed than that of the dogs brain.

THE CHANGES OF THE TESTOSTERONE HORMONE LEVEL DURING PREPUBERTAL PERIODE IN INDUCED HYPOTHYROIDISM RATS Teguh Budipitojo; Tri Wahyu Pangestiningsih
Jurnal Sain Veteriner Vol 16, No 1 (1998)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.8619

The one the influence factors to the normal functions and testes growth is thyroid gland status. The knowledge about the influences of euthyroid, and hypenhyroid to thetesies growth will help to give more understanding of the normal lestes growth. The new born male rats were used todetect the influence of hypothyroidism to the testosterone level during prepubertal periode. Twenty primigravida pregnant rats (Rattus norvegicus albinus) were used. After birth, the male pups were divided inio controls and treatments group. Mothers of the treatment group were given 0,1% 6-n-prapyl-2-thiauracil (PTU), via drinking water, ad libitum, after birth until on day 8. On dary 27, 30, 37, 44, and 51, five control pups and 5 treatment pups were sacrificed. The blood from each pup were collected to detec serum testosteron hormone level using immunoradLometric assay (IRMA) method. The research showed that serum testosteron level ofthe; treated group increased on day 30 (P<0,05) and day 37(P<0,05). Theresultof This research showed that PTU induced hypothyroidism produced increasing serum testosterone level.

EFFECTS OF RIBOFLAV1N SUPPLEMENTATION ON THE TERATOGENIC EFFECTS OF SODIUM BORATE IN WHITE RATS Tri Wahyu Pangestiningsih; Sumiyati Sunaryo
Jurnal Sain Veteriner Vol 14, No 2 (1995)
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Na-borate gave teratogenic effects both to rats and chickens. In chickens the fetal malformations caused by borate treatments were similar to those due to riboflavin deficiency. It was proposed that in embryonated eggs borate built a bond with riboflavin, so that riboflavin could not junction properly. This present investigation was conducted to assess the possibility of riboflavin in prevent the teratogenic effects of Na-borate in rats.Nineteen primigravida Wistar rats were allocated randomly to one of treatment groups, i. e, 9 rats were orally treated with 180 mg/200g BW/day, No-borate the other 10 rats beside treated with Na-borate, they were also treated orally with 2.5 mg/rat/day riboflavin. The treatments were done during the pregnancy on day 6 to 15. On day 20 the pregnant does were caesarean sectioned under ether anaesthesia. After all fetal malformations were noted, then all fetuses were placed in alcohol 70%, prepared for alizarin method for microscopic skeleton inspection. Chi square analysis of the gross inspection malformations, i e. the resorbed vs. the unresorbed, the alive vs. the dead, the haemorrhage vs. the non Hemorrhage fetuses of the two treatments showed unsignificant difference. Riboflavin supplementation increased singnificantly the number of fetuses with omphalocel. T-test analysis indicated that riboflavinsupplementation highly significantly caused the fore and hind paw, costal, sternal defects became more serious, if also significantly reduced the weight and the length of the fetuses. This investigation revealed that riboflavin supplementation failed to prevent, but even caused the teratogenic effects of the Na-borate to rats became more serious.

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani dengan Metode Imunohistokimia Avidin-Biotin-Peroxidase Complex Teguh Budipitojo; Ariana Ariana; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose Transporter 4 (GLUT 4) merupakan protein transporter glukosa yang diatur oleh insulin, ditemukan terutama di jaringan adiposa dan otot lurik (baik otot skelet maupun otot jantung). Ayam kedu cemani merupakan ayam endemik Indonesia yang terdapat di wilayah Kedu, Temanggung, Jawa Tengah. Penelitian ini diperlukan untuk melengkapi dokumentasi data anatomi mikroskopik kekayaan hayati asli Indonesia. Penelitian ini bertujuan untuk mengklarifikasi distribusi GLUT 4 dalam serabut otot skelet ayam kedu dengan metode imunohistokimia avidin-biotin-peroxidase complex (ABC). Penelitian dilakukan dengan menggunakan jaringan otot yang meliputi otot pectorales mayor, biceps brachii, dan biceps femoris dari 5 ekor ayam kedu cemani. Hasil penelitian menunjukkan bahwa immunoreaktivitas GLUT 4 terutama terdeteksi di sarkolema dan komponen miofibril pada otot pectorales mayor, biceps brachii, maupun biceps femoris. Intensitas imunorekatifitasnya meningkat dari intensitas lemah pada otot pectorales mayor, menjadi intensitas sedang pada otot biceps brachii, dan kemudian intensitas kuat pada otot biceps femoris. Hasil penelitian memunculkan dorongan untuk menggali lebih lanjut ciri-ciri khusus ayam kedu cemani lainnya, untuk mendokumentasi data anatomis kekayaan hayati asli Indonesia.

Studi Distribusi Glukosa Transporter 4 pada Otot Skelet Ayam Kedu Cemani Teguh Budipitojo; Ariana -; Tri Wahyu Pangestiningsih; Hery Wijayanto; Dwi Liliek Kusindarta; Dewi Kania Musana
Jurnal Sain Veteriner Vol 35, No 2 (2017): Desember
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Glucose transporter (GLUT 4) is glucose transporter protein regulated by insulin, found in adipose tissue and striated muscle (skeletal and cardiac muscle). Kedu cemani chicken is one of Indonesia endemic animal, found in Kedu, Temanggung regency, Central Java. This study was required to complete microscopic documentation of Indonesia’s native biodiversity. The objective of this study was to clarify GLUT 4 distribution in skeletal muscle fibers of kedu cemani chicken by using avidin-biotin-peroxidase complex (ABC) immunohistochemistry method. This study was conducted by using pectorales major, biceps brachii, and biceps femoris muscle tissue from 5 kedu cemani chicken. The result showed that GLUT 4 immunoreactivity were detected in sarcolemma and myofibrils component of pectorales major, biceps brachii, and biceps femoris muscle tissue. Intensity of GLUT 4 immunorectivites increased from weak intensity in pectorales major muscle tissue, moderate intensity in biceps brachii muscle tissue, then strong intensity in biceps femoris muscle tissue. This result might motivate to further exploration about the other kedu cemani chicken specific features to complete microscopic documentation of Indonesia’s native biodiversity.

Distribusi neuron serotonergik pada hipokampus Lasiwen (Myotis sp) sebagai satwa liar yang berpotensi menjadi reservoir virus rabies Tri Wahyu Pangestiningsih; Ariana Ariana; Irma Padeta; Arvendi Rahma Jadi; Woro Danur Wendo
Jurnal Sain Veteriner Vol 37, No 1 (2019): Juni
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Myotis sp is an Indonesian microchiropteran, insectivore bat that potential to be a reservoir for rabies virus. Rabies is fatal viral encephalitis and bat could act as wildlife potential reservoirs for the rabies virus and can transmit the disease to humans as the zoonotic disease. Hippocampus is one of diagnostic tissue for rabies disease and serotonergic neuron could involved in pathogenesis of rabies disease. The aim of the study is to investigate the distribution of serotonergic neurons in Myotis sp hippocampus. Five Myotis sp. were captured from wild population in Central Java, and were humanly anesthetized using ketamine and xylazin. The animals were perfused intracardially using NaCl 0.9% as the pre-rinse followed by 10% formaldehyde to fix it. The cerebrums were collected and processing the for paraffin embedding. Cerebrums were sectioning in saggital sections, 12 µm thickness serially with 120 µm intervals. The tissues were staining immunohistochemistry using antibody to serotonin (1/300; Bioss, Cat. No: bs-1126R) 2 night incubation in 4 oC temperature. The solution for blocking background, secondary antibody, avidin-biotin-peroxidae complex and chromogen using kit Starr Trek Universal HRP Detection System (Biocare Medical, Cat No: STUHRP700) and were analyzed descriptivelly. The results show that serotonergic neuros were distributed in the all area of the of Myotis sp hippocampus. In dentate gyrus neuron serotonergic (Sert-IR)s are round in shape and mostly distributed in the middle layer, few in the superficially also deeper layers. In the hippocampus , the Sert-IR neurons are pyramidal in shape and distributed in the CA1, Ca2 and CA3 areas. In subiculum, the Sert-IR neurons are pyramidal in shape, more wider distributed than in the CA1 with no differences between outer layer and deeper layer. The conclutions of this research are the serotonergic neurons are distributed in the all area of hippocampus

The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model Budi Susilo; Cahya Yustisia Hasan; Bambang Dwi Raharjo; Tri Wahyu Pangestiningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i8.338

Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14.

The Effect Application of Low Intensity Pulse Ultra Sound on Wounds Healing and Angiogenesis of Diabetes Mellitus Type 2 Rat Model Budi Susilo; Cahya Yustisia Hasan; Bambang Dwi Raharjo; Tri Wahyu Pangestiningsih
Bioscientia Medicina : Journal of Biomedicine and Translational Research Vol. 5 No. 8 (2021): Bioscientia Medicina: Journal of Biomedicine & Translational Research
Publisher : HM Publisher

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32539/bsm.v5i8.338

Wound healing disorder are often found in people with diabetes mellitus. Low Intensity Pulse Ultrasound (LIPUS) therapy was developed to accelerate the wound healing process. The purpose of this study was to determine the effect of LIPUS on wound closure and angiogenesis after skin excision in rats model diabetes mellitus type 2.Twenty-four Sprague dawley rats that match the inclusion criteria were made into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then devided into six group: DM3, DM7, DM14, DML3, DML7, DML14, with 4 rats each. All rats were made excision wound with punch biopsy in the back area. DML group receive LIPUS therapy in the wound area (frequency 3 MHz, intensity 0,5 W/cm 2 , duty cycle 20%, duration 3 minutes every day) for three days (DML3), seven days (DML7) and fourteen days (DML14). Wound area was measured and the tissue was staineing with hematoksciline eiosin to observe the density of angiogenesis.Two Way ANOVA used to to analyze the differences in the variable wound area and angiogenesis density, Post Hoc LSD to analyze the differences in variables between groups and the day of observation, showed the area of the wound in the DML was lower than in the DM group significant on day 3 and 7, whereas the density of the angiogenesis in DML group was higher than DM group significant on day 7 and 14. The Pearson test showed a correlation between the wound closure and the angiogenesis density (p = 0,000; r = -0,785). Conclusion: LIPUS therapy accelerate wound closure on day 3 and 7and increase the density of angiogenesis on day 7 and 14.

Effect of low-intensity pulsed ultrasound on mast cell degranulation and fibroblast expression on type 2 diabetes mellitus rats wound healing process Intan Maharani; Cahya Yustisia Hasan; Bambang Dwirahardjo; Tri Wahyu Pangestiningsih
Majalah Kedokteran Gigi Indonesia Vol 7, No 2 (2021): August
Publisher : Faculty of Dentistry, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/majkedgiind.59132

Impaired wound healing is one of the Diabetes mellitus complications. Low-intensity Pulsed Ultrasound (LIPUS) therapy may accelerate the impaired wound healing. The use of LIPUS therapy in the early inflammatory phase can induce mast cell degranulation, and in the proliferative phase it can increase collagen synthesis by fibroblasts. The purpose of this study was to determine the effects of LIPUS therapy on mast cell degranulation and fibroblastexpression in the healing process of punch biopsy wound in rats with type 2 diabetes mellitus. Twenty-four Sprague dawley (n=24) were designed into type 2 diabetes mellitus by injecting Nicotinamide and Streptozotocin, then divided into six groups: diabetes mellitus without LIPUS (DM3, DM7, DM14) and diabetes mellitus with LIPUS (DML3, DML7, DML14), 4 each, and punch biopsy wounds were made on the dorsal skin. The DML group received LIPUS therapy in the wound area (frequency 3 MHz, intensity 0.5 W/cm2, duty cycle 20%, duration 3 minutes every day for 3 days (DML3), 7 days (DML7), and 14 days (DML14). The wounded tissue area was stained with toluidine blue to observe mast cell degranulation and immunohistochemical type HSP-47 to observe fibroblast expression. Two-Way ANOVA and Post Hoc LSD tests were used to determine the differences in mast cell degranulation and fibroblast expression. The results showed that mast cell degranulation and fibroblast expression in the DML group were higher than in the DM group (table 1). Pearson test showed a correlation between mast cell degranulation and fibroblast expression (p=0.00; r= 0.839). LIPUS therapy increases mast cell degranulation and fibroblast expression in type 2 diabetes mellitus rat model. The higher the mast cell degranulation, the higher fibroblast expressions.

PENDAMPINGAN PEMBELAJARAN BIOLOGI ANATOMI HEWAN SECARA DARING UNTUK SEKOLAH-SEKOLAH DASAR GUGUS VI DI DESA TUKSONO, SENTOLO, KULON PROGO, YOGYAKARTA : Online Animal Anatomy Biology Learning Assistance for Cluster VI Elementary Schools in Tuksono Village, Sentolo, Kulon Progo, Yogyakarta Arvendi Rachma Jadi; Hery Wijayanto; Dwi Liliek Kusindarta; Vista Budiariati; Tri Wahyu Pangestiningsih; Teguh Budipitojo; Ariana; Hevi Wihadmadyatami
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 3 No. 2 (2022): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v3i2.301

ABSTRACT The problem faced during the new normal period of the Covid-19 pandemic for students is to restore interest in learning that has been done at home. Additional efforts are needed to reduce these concerns, such as mentoring for learning outside of school. Community service in the form of learning assistance is carried out with the aim of providing new experiences, information, and knowledge outside of learning at school. The schools participating in the service activities are schools from Cluster VI Tuksono Village, Sentolo, Kulon Progo, Yogyakarta. This activity involves student assistants from the Anatomy Department, Faculty of Veterinary Medicine, Universitas Gadjah Mada with assistance for a certain period. Implementation of online activities providing materials and discussions that have been adapted to the competencies needed in schools. The material given is an introduction to vertebrates macroscopically and microscopically. Students will be divided into several classes and each has an assistant and a co-lecturer. The activity takes place from September to November 2021. The results of the questionnaire show that this learning assistance activity is very interesting (60%) and very useful (69%) for students and teachers in their schools. Keywords: Animal, Biology Anatomy; Elementary school; Learning; Online ABSTRAK Permasalahan yang dihadapi pada masa normal baru pandemi Covid-19 bagi peserta didik adalah mengembalikan minat belajar yang selama ini dilakukan di rumah. Usaha untuk mengurangi keprihatinan tersebut diperlukan seperti pendampingan belajar luar sekolah. Pengabdian kepada masyarakat berupa pendampingan pembelajaran dilaksanakan dengan tujuan memberikan pengalaman, informasi, dan pengetahuan baru di luar pembelajaran di sekolah. Sekolah yang berpartisipasi dalam kegiatan pengabdian adalah sekolah-sekolah dari Gugus VI Desa Tuksono, Sentolo, Kulon Progo, Yogyakarta. Kegiatan ini melibatkan asisten mahasiswa Departemen Anatomi Fakultas Kedokteran Hewan Universitas Gadjah Mada dengan pendampingan selama periode tertentu. Pelaksanaan kegiatan daring pemberian materi dan diskusi yang sudah disesuaikan dengan kompetensi yang dibutuhkan di sekolah. Materi yang diberikan berupa pengenalan vertebrata secara makroskopik dan mikroskopik. Peserta didik akan dibagi menjadi beberapa kelas dan masing-masing memiliki satu asisten dan dosen pendamping. Kegiatan berlangsung dari bulan September sampai November 2021. Hasil kuisioner menunjukkan kegiatan pendampingan pembelajaran ini sangat menarik (60%) dan sangat bermanfaat (69%) bagi para peserta didik dan guru di sekolahnya. Kata kunci: Anatomi Biologi; Daring; Hewan; Pembelajaran; Sekolah Dasar

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