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Madi Hartono

Jurusan Peternakan Fakultas Pertanian Universitas Lampung, Bandar Lampung

Author-ID : 277072

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Education Engineering Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences Veterinary Other

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PENGARUH PROBIOTIK TERHADAP KOLESTEROL DARAH PADA AYAM PETELUR (LAYER) (Effect of Probiotik on Blood Cholesterol in Laying Hens) Sumardi Sumardi; Sutyarso Sutyarso; Gregorius Nugroho Susanto; Tintin Kurtini; Madi Hartono; Rr. Etty Puspitaningsih N.W.
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

The aim of this research was to find out the effect of probiotic on blood cholesterol in laying hens. Materials used were laying hens (Isa Brown strain) 43 weeks old and ration using probiotics. This research was conducted by using completely randomized design (CRD). The advanced research was done with testing the local probiotic with various concentration treatment, they are P0 (ration without probiotic), P1 (ration + 1% local probiotic), P2 (ration + 2% local probiotic), and P3 (ration + 3% local probiotic) with 5 time repetitions and in every repetitions consists of 2 chicken. Variables that were observed in the advance research were laying hens’s blood cholesterol that consists of total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides, and total leucocyte. The level of total cholesterol on P0, P1, P2, and P3 were 129.00±38.26, 116.00± 26.53, 121.00±33.72, and 137.00±52.53 mg/dL (P0.05) respectively; the HDL level were 50.60±16.47, 35.80±11.28, 59.40±19.74; 56.80±30.56 mg/dL (P0.05); the LDL level were 100.20±49.53, 39.20±17.14, 104.60±51.23, and 96.80±66.22 mg/dL (P0.05), respectively; the trigycerides level were 862.00±355.5, 533.00±146.1, 829.00±242.6, and 810.00±304 mg/dL (P0.05), respectively; and leucocyte count were 22.00±1.72, 21.00±3.79, 31.00±3.99, and 34.00±3.55 x103 mm3 (P0.01), respectively. In conclusion, the administrtion of local probiotic do not effect the blood cholesterol of layer chicken.

PENINGKATAN KUALITAS SEMEN KAMBING BOER DENGAN PEMBERIAN VITAMIN E DAN MINERAL Zn Sri Suharyati; Madi Hartono
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui pengaruh penambahan vitamin E dan mineral Zn terhadap kualitas semen kambing Boer. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) dengan empat perlakuan, yakni P0= tanpa penambahan vitamin E maupun mineral Zn; P1= pemberian vitamin E 50 IU/ekor/hari; P2= pemberian mineral Zn 25 mg/ekor/hari; P3= pemberian vitamin E 50 IU/ekor/hari dan mineral Zn 25 mg/ekor/hari. Penelitian menggunakan 12 ekor kambing berumur 3 tahun, masing-masing perlakuan menggunakan 3 ekor kambing. Data yang diperoleh dianalisis menggunakan analisis ragam dan dilanjutkan dengan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa pemberian vitamin E dan mineral Zn serta kombinasi keduanya tidak memengaruhi volume semen kambing Boer, tetapi berpengaruh terhadap motilitas dan persentase spermatozoa abnormal (P0,05) dan terhadap persentase spermatozoa hidup (P0,01). Pemberian mineral Zn dan campurannya dengan vitamin E mampu meningkatkan kualitas spermatozoa kambing Boer.

PRESERVASI DAN KRIOPRESERVASI SEMEN SAPI LIMOUSIN DALAM BERBAGAI BAHAN PENGENCER Sri Suharyati; Madi Hartono
Jurnal Kedokteran Hewan Vol 5, No 2 (2011): September
Publisher : Universitas Syiah Kuala

Penelitian ini bertujuan mengetahui jenis bahan pengencer yang paling baik untuk mempertahankan kualitas sperma sapi Limousin selama penyimpanan dan pembekuan. Dalam penelitian ini digunakan Rancangan Acak Kelompok ®(RAK) dengan 3 penggunaan bahan pengencer yaitu P =Andromed ; P =tris–kuning telur; P =susu skim, dan 4 kelompok 1 2 3 ®waktu koleksi sebagai ulangan. Hasil penelitian menunjukkan pengencer Andromed memberikan pengaruh terbaik ® terhadap kualitas spermatozoa. Motilitas spermatozoa dengan pengencer Andromed selama penyimpanan 18 jam, setelah ekuilibrasi, dan setelah thawing masing-masing adalah 61,45; 73,13; dan 48,13%. Persentase spermatozoa hidup dengan ® pengencer Andromed selama penyimpanan 18 jam, setelah ekuilibrasi, dan setelah thawing masing-masing adalah 83,25; ® 81,88; dan 55,53%. Persentase spermatozoa abnormal dengan pengencer Andromed selama penyimpanan 18 jam, setelah ekuilibrasi, dan setelah thawing masing-masing adalah 4,60; 10,73; dan 11,70%.

THE EFFECTIVENESS OF VITAMIN C AND E ADDITION IN VARIOUS DILUENTS OF BRAHMAN CATTLE SPERMATOZOA AFTER FREEZING Suharyati, Sri; Hartono, Madi; Siswanto, Siswanto; Nur Wahid, Agus; Rangga Saputra, Hanip; Nilam Sari, Fatma
Jurnal Kedokteran Hewan Vol 18, No 3 (2024): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v18i3.37128

This research aimed to determine the quality of Brahman cattle spermatozoa after freezing in various diluent ingredients (Egg Yolk Citrate, Egg Yolk Tris, Skim Milk) which added vitamin C and vitamin E. The research was carried out using 4 treatments with 3 different diluent ingredients, each of which was Vitamins C and E are added to each diluent ingredient at a dose adjusted to the type of diluent agent. The treatment given is P0: Diluent without Vitamin C and E; P1: Diluent + Vitamin C; P2: Diluent + Vitamin E; and P3: Diluent + Vitamin C and Vitamin E. Research data were analyzed using analysis of variance and followed by Duncan's test at the 5% and/or 1% level for different variables. The results showed that the addition of vitamin C and vitamin E had a significant effect (P0.05) on motility and viability in the egg yolk tris diluent and egg yolk citrate but had no significant effect (P0.05) in the skim milk diluent. The use of different diluents had no significant effect (P0.05) on motility, viability, and abnormalities. This research concludes that Vitamin C added to the diluent tris egg yolk, egg yolk citrate, and skim milk showed higher average motility, percentage of live spermatozoa, and abnormalities of post-freezing Brahman cow spermatozoa.

THE EFFECT OF BLACK CUMIN (Nigella sativa) SUPPLEMENTATION THROUGH DRINKING WATER ON THE HISTOLOGY OF SMALL INTESTINE AND LARGE INTESTINE OF BROILER CHICKENS Hartono, Madi; Santosa, Purnama Edy; Ermawati, Ratna; Sirat, Muhammad Mirandy Pratama
Jurnal Kedokteran Hewan Vol 15, No 3 (2021): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v15i3.19774

This study aimed to determine the effect of black cumin (Nigella sativa) supplementation through drinking water on the histology of broiler chickens small intestine and large intestine. The research was conducted from April-September 2020 in a cage facility of the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung. This research used a completely randomized design with four treatment groups and three replications (five broilers per replication) with a total of 60 male broilers. The treatments were drinking water without Black Cumin (P0, control); drinking water with Black Cumin 36 mg/kg BW/day (P1); 72 mg/kg BW/day (P2); and 144 mg/kg BW/day (P3). Three broilers from each group were randomly necropsied at 31 days old, and samples of the small intestine (duodenum, jejunum, and ileum) and large intestine were fixed with 10% formalin solution and sent to the Lampung Disease Investigation Center for histological preparations. The observation of preparations was carried out microscopically using the Leica DM500 Binocular Microscope to accurately calculate various parameter sizes. The results were analyzed statistically with one way Analysis of Variance at significant level 5% and if proven significant, then a Tukey test was conducted. The results of this study were that the supplementation of black cumin 72 mg/kg BW/day through drinking water could significantly increase (P0.05) 1) the average sizes of villi height and villi area of small intestine (duodenum, jejunum, ileum) and large intestine (colon) of broiler chickens; 2) the average sizes of basal villi width, villi apex width, and gland diameter of broiler duodenum; and 3) the average size of apex villi width of broiler colon. The conclusion of this study was the supplementation of black cumin at dose of 72 mg/kg BW/day through drinking water could increase the histological sizes of the small intestine and large intestine of broilers.

FEEDING LOCAL FERMENTED WITH EM-4, AMMONIUM SULFATE, AND UREA ON THE BLOOD FAT LEVEL OF MALE DUCK Septinova, Dian; Arti, Aisyah Yuli; Sutrisna, Rudy; Tantalo, Syahrio; Hartono, Madi
Jurnal Kedokteran Hewan Vol 14, No 2 (2020): June
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v14i2.16501

The objective of this study was to determine the effect of commercial ration by the addition of ammonium sulfate and urea fermented feed ingredients on the blood fat levels of local male ducks. This study used a completely randomized design with 32 male ducks which weredivided into four groups namely R0, R1, R2, R3, and R4. Each group was given the following treatment: R0 only given commercial ration;R1 was given 80% commercial ration + 20% fermentation of EM-4 + 1.5% urea); R2 was given 80% commercial ration + 20% EM-4 + 1.5%ammonium sulfate fermentation; and R3 was given 80% commercial ration + 20% EM-4 + 1.5% urea + 1.5% ammonium sulfate. Variablesin this study were blood fat consisting of cholesterol, triglyceride, High-Density Lipoprotein (HDL), and Low-Density Lipoproteins (LDL).The data obtained were analyzed by using 5% real level and LSD. The results showed that the provision of commercial ration added withlocal feed fermented with ammonium sulfate and urea had a significant effect on cholesterol levels (P0.5), but not significant (P0.05) ontriglyceride, HDL, and LDL levels. Furthermore, the ration that gave the best effect on the blood fat levels of male ducks blood was the ration given to R3 group.

THE EFFECT OF MAGNETIC FIELD ON ANTIBIOTIC INHIBITION FOR Escherichia coli AND Bacillus sp. Sumardi, Sumardi; Agustrina, Rochmah; Ekowati, Christina Nugroho; Putra, Rizani Oktanisyah; Hartono, Madi
Jurnal Kedokteran Hewan Vol 13, No 1 (2019): March
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v13i1.8051

This study was aimed to test the growth of bacteria Escherichia coli (E. coli) and Bacillus sp. which were exposed to magnetic fields. In the first stage of the study, the effect of magnetic fields on the growth of E. coli and Bacillus sp were observed. The futher study was aimed to evaluate the effect of magnetic field on antibiotic sensitivity againts the growth of E. coli and Bacillus sp. The magnetic fields treatments were 0.0 mT (control), 0.1 mT, 0.2 mT, 0.3 mT which were exposed for 10 hours for each treatment. Five antibiotics (trimethoprim, ampicillin, nalidixic acid, streptomycin and chloramphenicol) were used for each bacteria. The result showed that the magnetic field did not influence the colony growth of E.coli, but in Bacillus sp. was seen the increasing of colony area in magnetic field of 0.1 mT and 0.2 mT compared with control treatment. Antibiotic of trimethoprim, nalidixic acid, and ampicillin increase the growth inhibition of E. coli when the bacteria have been exposed to 0.1 mT; 0.2 mT; 0.3 mT magnetic field for 10 minutes. The inhibition by streptomycin and chloramphenicol antibiotic on E. coli did not affected by magnetic field exposure. The inhibition of Bacillus sp by trimethoprim and ampicillin increased when the bacteria have been exposed to 0.2 mT and 0.3 mT magnetic field for 10 minutes. The inhibition of nalidixic acid, streptomycin and chloramphenicol to Bacillus sp. did not affected by the magnetic field exposure.

PHYSIOLOGICAL RESPONSES OF SABURAI GOAT ON THE OF ADDITION DIETARY PROTEIN LEVEL Adhianto, Kusuma; ., Muhtarudin; Sulastri, Sulastri; Hartono, Madi
Jurnal Kedokteran Hewan Vol 11, No 3 (2017): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v11i3.4352

The purpose of this research was to study the effect of different level of protein addition in goat ration on feed addition in the ration on feed intake and physiological responses of Saburai goats (rectal temperature, respiratory rate, and heart rate). Twenty male Saburai goats were randomly divided into 4 treatments group with 5 goats each. Goats in group 1 (PO) were fed with forage, while goats in group 2 (P1), 3 (P2), and 4 (P3) were fed with forage + concentratre with 13%, 16%, and 19% crude protein, respectively. The treatments were P0= feeding forage, P1= feeding forage + concentrate with 13% crude protein (CP), P2= feeding forage + concentrate with 16% CP, and P3 feeding forage + concentrate with 19% CP. The results showed that the rectal temperature and heart rate did not significantly affected (P0.05) in all treatment, however the heart rate was significantly (P0.05) affected among the treatments. The conclusion is the addition of different protein level in the ration has no effect on rectal temperature and respiratory rate, but significantly increases the heart rate frequency.

PENGARUH PROBIOTIK TERHADAP KOLESTEROL DARAH PADA AYAM PETELUR (LAYER) (Effect of Probiotik on Blood Cholesterol in Laying Hens) Sumardi, Sumardi; Sutyarso, Sutyarso; Susanto, Gregorius Nugroho; Kurtini, Tintin; Hartono, Madi; Puspitaningsih N.W., Rr. Etty
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v10i2.5042

The aim of this research was to find out the effect of probiotic on blood cholesterol in laying hens. Materials used were laying hens (Isa Brown strain) 43 weeks old and ration using probiotics. This research was conducted by using completely randomized design (CRD). The advanced research was done with testing the local probiotic with various concentration treatment, they are P0 (ration without probiotic), P1 (ration + 1% local probiotic), P2 (ration + 2% local probiotic), and P3 (ration + 3% local probiotic) with 5 time repetitions and in every repetitions consists of 2 chicken. Variables that were observed in the advance research were laying henss blood cholesterol that consists of total cholesterol, high density lipoprotein (HDL), low density lipoprotein (LDL), triglycerides, and total leucocyte. The level of total cholesterol on P0, P1, P2, and P3 were 129.0038.26, 116.00 26.53, 121.0033.72, and 137.0052.53 mg/dL (P0.05) respectively; the HDL level were 50.6016.47, 35.8011.28, 59.4019.74; 56.8030.56 mg/dL (P0.05); the LDL level were 100.2049.53, 39.2017.14, 104.6051.23, and 96.8066.22 mg/dL (P0.05), respectively; the trigycerides level were 862.00355.5, 533.00146.1, 829.00242.6, and 810.00304 mg/dL (P0.05), respectively; and leucocyte count were 22.001.72, 21.003.79, 31.003.99, and 34.003.55 x103 mm3 (P0.01), respectively. In conclusion, the administrtion of local probiotic do not effect the blood cholesterol of layer chicken.

PENINGKATAN KUALITAS SEMEN KAMBING BOER DENGAN PEMBERIAN VITAMIN E DAN MINERAL Zn Suharyati, Sri; Hartono, Madi
Jurnal Kedokteran Hewan Vol 7, No 2 (2013): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v7i2.897

Penelitian ini bertujuan mengetahui pengaruh penambahan vitamin E dan mineral Zn terhadap kualitas semen kambing Boer. Rancanganyang digunakan adalah rancangan acak lengkap (RAL) dengan empat perlakuan, yakni P0= tanpa penambahan vitamin E maupun mineral Zn; P1= pemberian vitamin E 50 IU/ekor/hari; P2= pemberian mineral Zn 25 mg/ekor/hari; P3= pemberian vitamin E 50 IU/ekor/hari dan mineral Zn 25 mg/ekor/hari. Penelitian menggunakan 12 ekor kambing berumur 3 tahun, masing-masing perlakuan menggunakan 3 ekor kambing. Data yang diperoleh dianalisis menggunakan analisis ragam dan dilanjutkan dengan uji beda nyata terkecil (BNT). Hasil penelitian menunjukkan bahwa pemberian vitamin E dan mineral Zn serta kombinasi keduanya tidak memengaruhi volume semen kambing Boer, tetapi berpengaruh terhadap motilitas dan persentase spermatozoa abnormal (P0,05) dan terhadap persentase spermatozoa hidup (P0,01). Pemberian mineral Zn dan campurannya dengan vitamin E mampu meningkatkan kualitas spermatozoa kambing Boer.

Co-Authors Abimanyu Prastyo Ardiansyah Absor, Ulil Adek Rayhan Regisa Adhitia, Fabian Agnes Sekar Weningtiyas Agung Kusuma Wijaya Agustin, Nur Aini Ajmal Kurniawan Khair Akbar, Muhamad Tri Al-amin, Ahmad Fauzy Alhuda, Dinda Yusri Ali Husni Ali Husni Ambya, Ambya Amita Juliana Ardhyka Chandra Stefanus Arief Rahmaan Hakim Arif Darmawan Arif Irawan Arif Kurniadi Arif Qisthon Arrozak, Muhammad Rofif Ilham Arti, Aisyah Yuli Ayu Lidyana Bastian Rusdi Bayu Hadi Setya Irawan Beninda, Adellia Christina Nugroho Ekowati, Christina Nugroho Cici Hardiyanti Dakhlan, Akhmad Dewi Wijayanti Dian Septinova Dwi Haryanto Dwi Rismawati edy santosa, purnama Ertha Colanda Sari Erwanto Etha 'Azizah Hasiib Ety Apriliana Fajar Fitraldi Dirgahayu Fandi Abdillah Farida Fathul Farida Fathul Fatmawati, Siti Tika Fauzi, Teo Achmad Febriyansar, Eri Fitria Tsani Farda Frandy Febrianthoro Fransisca, Amaylia Habiahan, Renta Enjelina Hadi Prayitno Hanafi, Riyan Hanawati, Nadia Dwi Hanip Rangga Saputra Harry Setiawan Henry Wijaya, Henry Herdiansyah, Lani Hermawan, Alan Ida Ayu Putu Sri Widnyani Iis Nurlia Imam Widodo Irmaylin SM Jenny Marthika Sari Juandita, Karina Natasya Konita Lutfiana, Konita Kristanti, Nuke Kurotul Aini Kusuma Adhianto Kusuma Adhianto Larasati, Hindun Legowo, Ahmad Bambang Lidyana, Ayu Liman Liman, Liman Lumbantoruan, Neny Santy Jelita Lusia Komala Widiastuti Lutviati, Carmelia M. Dima Iqbal Hamdani Maisaroh, Siti Maisaroh Siti Malau, Regina Wati Maulinda, Riska Mia Widowati Miranti Olivia Mokhamad Aiyon Suharis Monika, Lidiya Muhamad Allabus Royan Muhammad Daffa Ewaldi Muhammad Fadhil Muhammad Fauzan Muhammad Mirandy Pratama Sirat Muhtaruddin, Muhtaruddin Muhtarudin Muhtarudin Mulyadi, Taufik Munzir, Indah Iftinandari Nabila, Amara Nilam Sari, Fatma Ningrum, Tiara Arnenda Diah Novigar, Muhammad Fikri Nugraha, Ega Nugraha, Resta Eka Nugroho, Kaleb Wisnu Nur Wahid, Agus Nurul Hakiki Pubiandara, Sintha Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Purnama Edy Santosa Puspitaningsih N.W., Rr. Etty Putra, Alvin Permana Putra, Rizani Oktanisyah Putri Omelia Rahmadhanil Putra Rusadi Ramadhan, Muhammad Elvin Rangga Saputra, Hanip Ratna Ermawati Renaldy, Yogie Ridwan Ririn Angriani Rito, Hardiansyah Faisal Rivai, Mahfud Riyan Hanafi Riyanti Riyanti . Riyanti Riyanti Rizki Indah Pratiwi Rizkiyah, Nayla Syaqiratul Rochmah Agustrina Rohmatin Nisak Rojab Fadillah Rr. Etty Puspitaningsih N.W. Rudy Sutrisna Rudy Sutrisna S Siswanto Sagala, Yollanda Natalia Sakila, Fanya Putri Saputra, Andi Kurnia Saputra, Mahmud Yoga Saputri, Rafida Bela Saputri, Rhica Dhea Sari, Siti Hartika Saturdayana, Made Segara, Rendi Bayu Selawati, Diyah Septianisa, Septianisa Sherly Puspa Ningrum Sihombing, Paulus Ardiansyah Silvia, Desma Siswanto Siswanto . Siswanto Siswanto Siswanto Siswanto Sri Andayani Sri Ratna Sulistiyanti Sri Suharyati Sri Suharyati Sri Suharyati Sri Suharyati Sri Suharyati Sri Suharyati Sri Suharyati Suhono Suhono, Suhono Sulastri Sulastri Sumardi . Sumardi, Sumardi Surmini Surmini Susanto, Gregorius Nugroho Sutyarso Sutyarso Sutyarso Sutyarso Syahrio Tantalo Syahroni Syahroni Syamsul Arifin Teguh Rafian, Teguh Tintin Kurtini Tintin Kurtini Tintin Kurtini Tri Nurjanah Ulvi Fitri Handayani Veithzal Rivai Zainal Veronica Wanniatie Wijaya, Agung Kusuma Yohanes Narendra Darma Atmaja Yudhistira, Guntur Januar Yuli Prasetyo Zulfi Al Arif Zulvina Afrianti

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