Claim Missing Document
Check
Articles

Development of Microbiology Handout as Teaching Material to Improve Science Process Skills of Health Vocational Students Pratama, Aris Yudha; Hastuti, Utami Sri; Setiowati, Frida Kunti
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.49473

Abstract

This research is a research and development of Microbiology Handout on the topic "Antibacterial Test on Bacterial Growth Inhibition" which uses natural materials, namely star fruit extract (Averrhoa carambola L.), which will be used as teaching materials in the learning process to help improve students' science process skills. The purpose of developing Microbiology Handout is to produce teaching materials that are valid, practical and effective for use in learning Microbiology subjects. Microbiology Handout development uses the ADDIE development model. Validation of Microbiology Handouts was carried out by learning media and teaching material expert validators, Microbiology material experts, and Microbiology learning practitioner expert validators. The Microbiology Handout trial was conducted through three stages of trials, namely a one-to-one trial of 3 students, a small group trial of 10 students, and a field trial of 15 students. The effectiveness of Microbiology Handout in improving students' science process skills can be seen through pretest and posttest scores analyzed using the IBM SPSS version 26 application through the One Way Ancova test. The results of research and development show the acquisition of validation scores by learning media and teaching material experts of 100%, Microbiology material experts of 94.3%, education practitioner experts of 99.64%, student response results of 91.43% and efficiency results of 0.028
Microbiology literacy and its influence on knowledge, perceptions and community attitudes Mustofa, Ali; Hastuti, Utami Sri; Susanto, Hendra
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 1 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.49273

Abstract

Microbiology literacy development shapes people's attitudes, behaviors, and perceptions toward microorganisms. This study aims to explore the relationship between microbiological literacy and people's perceptions and attitudes towards microorganisms. This study uses the Systematic Literature Review (SLR) method to analyze the relationship between microbiological literacy and public knowledge, attitudes, and perceptions of microorganisms. Data were retrieved from Scopus using relevant keywords and then filtered based on the following criteria: published between 2019–2024, in English, and open access. The analysis was conducted through four steps: coding, concept review, and summarizing key information (author, year, and core concepts). The results were synthesized to map the relationship between microbiological literacy and public perceptions. The results showed that respondents' knowledge levels varied about various health issues. Good microbiological knowledge can improve health literacy, affecting individual attitudes and perceptions of health. Regarding respondents' attitudes, it is known that respondents are interested in education and health awareness. A positive attitude toward health can encourage actions that support public health. In contrast, individuals with low microbiology knowledge orientation are more likely to cause negative perceptions and distrust of health interventions. Continuous, evidence-based education is essential to improve microbiology and health literacy and shape positive attitudes toward health interventions. Microbiology knowledge has a broad impact on daily life and the future. However, there are significant challenges in understanding microbiology. Public perception of microbes is often only associated with 'germs' and negative images. Therefore, microbiology knowledge in learning must be presented in visual arts and visual, olfactory, and tactile experiences. The more we understand the role and knowledge of microbes, the better we can make relevant decisions.
Active Compounds and Antibacterial Activity of Banana (Musa paradisiaca) var. Agung Bunches and Combs Extracts Against Escherichia coli Analysis Pratama, Ade Wahyu; Hastuti, Utami Sri
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 2: Jurnal Pembelajaran Dan Biologi Nukleus June 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i2.7186

Abstract

Background: Escherichia coli cause diarrhea. E. coli infections can be treated using synthetic antibiotics. Natural antibiotics are an alternative for prevent E. coli infections as synthetyc antibiotic substitute. Natural antibiotics are come from plants part such as agung banana’s (Musa paradisiaca) bunch and combs. This bananas are used as a dessert fruits. The waste of this friut, such as bunches and fruit comb. This banana plant is potentially as antibacterial substance. The aims of this research are, 1) to examine the antibacterial effect of banana bunch and comb extracts; 2) to determine the effective concentration of the extract against the E.coli growth; and 3) to analysis of the active compounds content of banana bunch and comb extracts. Methodology: The antibacterial effect was examine using the agar well diffusion. Total active compound levels was examine using the UV-Vis spectrophotometry method and the derivative compounds uses the LCMS method. Findings: The research results showed the bunches had antibacterial effect against E. coli and the effective concentration is 90% with the highest inhibitory zone size diameter is 6.98 ± 0,00 mm, while the combs effective concentration is 80% is 6.55 ± 0.81 mm. The antibacterial effect of both extracts is moderate categorize. The highest content of active compounds in the bunches is phenol, with content: 70.70 mg/g and in the comb is 45.82 mg/g. Contribution: This research contributes to the utilisation of organic waste as an alternative source of environmentally friendly natural antibacterial agents and supports innovation in phytopharmaceuticals based on local plants.
Endophytic Fungi as A Symbion in Polianthes tuberosa L.: Identification, Microscopic Observation, and Secondary Metabolite Analysis Hastuti, Utami Sri; Abdini, Aulia; Puspitasari, Dela Reni; Hamidah, Rodiah Amin; Labibah, Sylvana Bilqis
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 11, No 3: Jurnal Pembelajaran Dan Biologi Nukleus September 2025
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v11i3.7928

Abstract

Background: Polianthes  tuberosa L. was known as an ornamental plants. People use this plant flower for ritual purpose because of the fragrant aroma. This plant rarely attacked by microorganism and usually stay healthy. Some endophytic fungi species live with P. tuberosaL. plant in mutualistic symbiosis interaction. This study was conducted to: (1) determine where the hyphae of endophytic fungi are located within the tissue of P. tuberosa L. plant petals and leaves, using findings from microscopic observations; (2) identify the species of endophytic fungi residing in the flower petals and leaves of P. tuberosa L.; and (3) ascertain the various secondary metabolites produced by each species of endophytic fungi. Methodology: P. tuberosa plant were obtained from flower market at Malang city. The leaves and flower petal parts were prepared for microscopic observation and identification. Cultures of each endophytic fungal species were grown on PDA plate medium, then cut into pieces measuring 5 x 1 cm and inoculated in PDB medium. These were shaken at a speed of 120 rpm for 7 days. After that, the liquid culture was centrifuged at 3000 rpm for 10 minutes. The contents of several secondary metabolites uses the supernatant of the liquid culture. The phytochemical analysis used spectrophotometry method.  Findings: The study findings are: (1) the endophytic fungi hyphae are located in stomata neighbor cells, the walls of xylem cells, epidermal cell walls, sponsa cell walls, and also within the petal epidermis cell walls of the flower; (2) eight endophytic fungi species have been identified; (3) every species of endophytic fungi generates flavonoids, alkaloids, tannins, saponins, and steroids, each with varying content levels. Contribution: Based on this research result, the endophytic fungi culture can be utilized as natural antibiotic source for better synthetic substitution. Besides that endophytic fungi also can be applied as antagonistic fungi to inhibite pathogenic fungi growth that attack some cultivated plant through appropriate research
Development of Guided Inquiry-Based Microbiology Modules Based on the Identification of Contaminant Molds in Tomato Fruit to Improve Students' Science Process Skills Naufal Wima Al Fahri; Utami Sri Hastuti; Safwatun Nida
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 3 (2025): Vol. 23, No. 3, October 2025
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i3.53749

Abstract

Students often experience difficulties in identifying microbiological specimens due to limitations in preparing and interpreting microscopic slides. This problem is evident in the context of tomato fruits (Solanum lycopersicum), which are widely consumed agricultural commodities but are highly perishable and susceptible to microbial contamination, especially by molds. To address this challenge, this study aims to develop a guided inquiry-based microbiology learning module using tomato mold identification as a case study, with the goal of improving students’ Science Process Skills (SPS). The research was conducted in two stages: (1) laboratory experiments to identify contaminant molds in tomatoes and (2) development of a learning module using the ADDIE model (Analyze, Design, Develop, Implement, Evaluate). The sample consisted of 62 microbiology students from the Biology Education Program, Faculty of Mathematics and Natural Sciences, State University of Malang. A non-randomized control-group pretest–posttest design was employed, the increase in posttest results compared to the pretest was calculated using the N-gain score. Results showed that students’ SPS was still relatively low, particularly in the observing indicators (30.43%) and hypothesizing indicators (43.48%). The developed module integrates real laboratory findings into structured inquiry-based activities, emphasizing hands-on practice, critical thinking, and contextual relevance to agricultural microbiology. Its distinctive features include the use of authentic microbial identification data, scaffolding through guided inquiry steps, and alignment with SPS indicators. This module is expected to provide an effective solution for enhancing students’ SPS in microbiology learning
Duration Effects of Shrimp Paste Storage on ALT of Mold Colonies: Variations by Brand and Pre-Standard Treatment Syafitri, Nur Laila; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4690

Abstract

Shrimp paste is a commonly employed condiment or flavoring in culinary preparations. As a result of the nutrients present in shrimp paste, fungi and other microbial contaminants are able to proliferate and develop on the paste. This study aims as follows: 1) to determine ALT measurements of mold colonies to assess the quality of two brands of pre-treated shrimp paste; and 2) to identify contaminating mold species present in steamed and unsteamed shrimp paste prior to storage. Descriptive quantitative and qualitative research methods were employed within the Biology Department of FMIPA UM's Microbiology Laboratory. A series of three samplings were conducted on treated shrimp paste brands A and B, one week apart between each sampling. A volume of 90 ml of 0.1% peptone water was used to dissolve 10 grams of shrimp paste, resulting in a dilution of 10-1; this process was repeated until the dilution reached 10-5. 0. The surface of PDA (Potato Dextrose Agar) medium was inoculated with 1 ml of suspension from each dilution. The medium was subsequently incubated at a temperature of 25oC for 7x24 hours. The findings of this study indicate the following: 1) At the 28th day of storage, both brands of shrimp paste that underwent treatment with steamed and unsteamed shrimp paste remained of acceptable quality for human consumption. 2) In steamed and unsteamed shrimp paste, thirteen species of mold contaminants were identified: Chrysosporium corda, Cladosporium sphaerospermum, Penicillium frequentans, Penicillium chrysogenum, Rhizoctonia sp1., Aspergillus candidus, Fusarium equeseti, Colletotrichum ti, Moniliella acetobutens, and Rhizoctonia sp3
Pemanfaatan limbah klobot salak monik sebagai nata-mocca untuk peningkatan ekonomi desa Utomo, Muhammad Andry Prio; Rakhmawati, Yunita; Daniarsih, Ajeng; Hastuti, Utami Sri; Lestari, Sri Rahayu; Izzalqurny, Tomy Rizky
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 8 No 3 (2025)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v8i3.22746

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan keterampilan dalam pembuatan nata menggunakan klobot salak Monik serta branding dan pemasarannya via online. Metode pelaksanaan pelatihan ini adalah learning service dengan pendekatan demonstrasi. Rangkaian acara pelatihan meliputi visitasi dan koordinasi dengan industri keripik salak, simulasi pembuatan nata salak Monik oleh tim, pelatihan kepada peserta, pengisian pre-test dan post-test, dan evaluasi pelatihan. Pelatihan ini diikuti oleh 20 peserta yang merupakan anggota PKK desa Tirtoyudo yang sebagian besar bekerja sebagai karyawati di pabrik industri keripik salak. Pelatihan menghasilkan 20 toples Nata-Mocca yang merupakan nata yang media fermentasinya menggunakan ekstrak limbah salak Monik. Produk Nata-Mocca tersebut telah dikemas secara menarik daa dipasarkan secara online melalui e-commerce Shopee. Berdasarkan hasil pengisian pre-test dan post-test yang dianalisis menggunakan T-test (p>0,05) menunjukkan hasil yang berbeda signifikan sehingga pelatihan efektif dalam meningkatkan pengetahuan dan keterampilan dalam pembuatan nata. Hasil evaluasi pelatihan menunjukkan semua indikator dinilai sangat baik oleh 80% peserta. Melalui pelatihan ini diharapkan produk Nata-Mocca olahan limbah klobot dapat dijadikan alternatif untuk peningkatan ekonomi warga desa Tirtoyudo.
Antagonism and mycoparasitism mechanism of T. harzianum against pathogenic fungus species of F. oxysporum and Capnodium sp. Mustofa, Ali; Hastuti, Utami Sri
Inornatus: Biology Education Journal Vol. 4 No. 1 (2024): Inornatus: Biology Education Journal
Publisher : Univeritas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30862/inornatus.v4i1.581

Abstract

The purpose of this study was to test the ability of T. harzianum to inhibit the growth of pathogenic mold species and to determine the effectiveness of T. harzianum mold species in inhibiting the growth of pathogenic molds, as well as investigate how T. harzianum and pathogenic molds interact with each other. The Microbiology Laboratory of the Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Malang, is where this research was conducted. The dual culture method was used for this test by using Czapek Agar (CA) medium. The isolated molds were then incubated for 4x24 hours at 25O–27OC. After that, the antagonism power was calculated. The results of macroscopic and microscopic observations were used to assess the mechanism of T. harzianum mold antagonism against pathogenic molds. The results showed that Trichoderma mold species were more resistant to the pathogenic mold Fusarium oxysporum than the pathogenic mold Capnodium. The antagonistic power of T. harzianum was 80%, with the antagonistic power of Capnodium sp. at 66.7%. The mechanism of mycoparasitism occurs when the hyphae of T. harzianum attach or entangle the hyphae of pathogenic molds, causing damage to the hyphal structure and inhibiting the growth of pathogenic molds.
Pengaruh Variasi Macam Gula dalam Beberapa Konsentrasi Terhadap Kualitas Nata De Nira Siwalan (Borassus Flabelliver L.) Pamekasan Madura Kirana, Chandra; Hastuti, Utami Sri; Suarsini, Endang
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (591.099 KB)

Abstract

Siwalan (Borassus flabellifer L.) tersebar luas di Kabupaten Pamekasan salah satunya di desa Kertagenah Laok Kecamatan Kadur.Potensi siwalan yang melimpah dapat dikembangkan menjadi produk yang lebih bermanfaat dan bernilai ekonomi tinggi salah satunya adalah dalam pembuatan nata de nira siwalan. Kualitas nata dapat dipengaruhi oleh beberapa faktor salah satunya adalah macam gula dan konsentrasi. Penelitian ini bertujuan menguji pengaruh variasi macam dan konsentrasi gula terhadap kualitas nata ditinjau dari kadar serat. Penelitian menggunakan Rancangan Acak Lengkap (RAL). Nira siwalan diperoleh dari penghasil nira di desa Kertagenah Laok Kecamatan Kadur Kabupaten Pamekasan Madura. Pembuatan nata dilakukan di Laboratorium Mikrobiologi Jurusan Biologi FMIPA Universitas Negeri Malang sedangkan pengujian kadar serat dilakukan di Laboratorium Kimia Universitas Muhammadiyah Malang. Prosedur penelitian meliputi: (1) perbanyakan starter; (2) pembuatan nata de nira siwalan; (3) penentuan kadar serat nata de nira siwalan. Analisis data yang digunakan adalah Analisis Varians (ANAVA) ganda yang dilanjutkan dengan uji DMRT 5%. Hasil penelitian menunjukkan bahwa : (1) jenis gula berpengaruh signifikan terhadap kadar serat nata de nira siwalan; (2) konsentrasi gula berpengaruh signifikan terhadap kadar serat nata de nira siwalan; serta (3) ada pengaruh interaksi jenis dan konsentrasi gula terhadap kadar serat nata de nira siwalan.
Daya Antibakteri Metabolit Kapang Endofit dari Tanaman Obat Ginseng Jawa (Talinum Paniculatum (Jaq.) Gaertn) Terhadap E.Coli dan B.Subtilis Hastuti, Utami Sri; Rahmawati, Indriana; Mastika, Laily Maghfiro Kamil; Asna, Putri Moortiyani Al; Sundari, Syifa
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2016: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (577.376 KB)

Abstract

Kapang endofit hidup di dalam tanaman inang, tetapi tidak bersifat patogen. Beberapa spesies diantaranya mampu menghasilkan metabolit sekunder yang bersifat antibiotik, sehingga mempunyai potensi dimanfaatkan sebagai bahan antibiotik alami. Penelitian ini bertujuan untuk : 1) menguji daya antibakteri metabolit kapang Aspergillus candidus, Fusarium semitectum, dan Fusarium lateritium terhadap E. coli ; 2) menguji daya antibakteri metabolit kapang A. candidus, F. semitectum, dan F. lateritium terhadap B. subtilis ; 3)menguji daya antibakteri metabolit campuran ketiga spesies kapang terhadap E. coli dan B. subtilis. Isolat-isolat kapang A. candidus, F. semitectum, dan F. lateritium diinokulasikan ke dalam medium Potato Dextrose Broth (PDB) dan dikocok dengan shaker dengan Kecepatan 120 rpm selama 7x24 jam, kemudian disentifugasi dengan kecepatan 3000 rpm. Supernatan dari masing-masing spesies kapang dan campuran ketiga spesies kapang diuji daya antibakterinya terhadap E. coli dan B. subtilis dengan metode difusi cakram. Hasil penelitian membuktikan bahwa ; 1) Metabolit masing-masing spesies kapang mempunyai daya antibakteri terhadap E. coli dan B. subtilis ; 2) Metabolit A. candidus mempunyai daya antibakteri yang tertinggi baik terhadap E. coli maupun B. subtilis ; 3) Metabolit campuran ketiga spesies kapang mempunyai daya antibakteri terhadapa E. coli dan B. subtilis.
Co-Authors *Ali Mustofa Abdini, Aulia Abdul Fattah Noor Abdul Ghofur Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Agung Witjoro Ahmad Najib Ajeng Daniarsih Ana Syarifatun Nisa Anggia Oktantia Anindya Nirmala Permata Arif Rahman Arini Zahrotun Nasichah Ary Maf’ula Asna, Putri Moortiyani Al Aswal Salewangeng Aziz Tanama Balqis Balqis Betty Lukiati Chandra Kirana Chandra Kirana Chandra Kirana Choirunnisa, Hesti Nur Chomisatut Thoyibah Dina Istia’nah Dwi Listyorini Dwi Rahmawati Dwi Rahmawati Dwi Rakhmawati Dwi Rakhmawati Endang Suarsini Erna Wijayanti Faiza Nur Imawati Ningsih Fatchur Rohman Fatchur Rohman Fatchur Rohman Febriani Sarwendah Asri Nugraheni Febriani Sarwendah Asri Nugraheni Fitri, Rizka Diah Fitria Maulita Fitria Maulita Fitriyah, Rofiqoh Lailatul Frida Kunti Setiowati Galina Istiqhfarini Gani, Abdul Rasyid Fakhrun Hadi Suwono Hamidah, Rodiah Amin Hanzen, W.F Edi Hendra Susanto Henny Nurul Khasanah Henny Nurul Khasanah Henny Nurul Khasanah Herawati Susilo Herdina Rahma Zalita Hesti Nur Choirunnisa Ibrohim Imam Mudakir Indah Sari Dewi Indriana Rahmawati Indriana Rahmawati Indriana Rahmawati Intan Rezki Kurniasari Istamar Syamsuri Izzalqurny, Tomy Rizky Izzatinnisa’ Izzatinnisa’ Khusnul Khotimah Kuniasari, Intan Rezki La Arlan Labibah, Sylvana Bilqis Lely Hermawati Linda Hapsari Linda Hapsari M. Ainur Yaqin M. Amin M. Nidhamul Maulana Mafluhah, Luluk Rofiatul Mahesti, Tika Maknuna, Durrotul Mareta Arisswara Edy Mariyanti Mariyanti Mashuri Saputra Mastika, Laily Maghfiro Kamil Maya Firdausi Prayudhani Muhammad Andry Prio Utomo Muhammad Syamsussabri Murni Sapta Sari Natalia Rosa Keliat Naufal Wima Al Fahri Ningsih, Husdiani Nugraheni, Febriani Sarwendah Asri Nursyahbani Saraha Nurul Yanuarsih Nuzalifa, Yossie Ulfa Otavia Dewi Permata Ika Hidayati Pratama, Ade Wahyu Pratama, Aris Yudha Puspitasari, Dela Reni Putri M. Al Asna Putri Moortiyani Al Asna Qorry Aulya Rohmana Rahel Natalia Saragih Munthe Rahmadyah Kusuma Putri Ria Yustika Sari Ria Yustika Sari Ria Yustika Sari Rina Kristina Maria Rizky, Mirza Yanuar Rofiqoh Lailatul Fitriyah Rozana, Kennis Safrudin M. Abidin Saidil Mursali Saidil Mursali Sari, Ria Yustika Siti Aisaroh Siti Annisaa'ul Kariimah Siti Hartina Pratiwi Siti Zubaidah Siti Zubaidah Sitoresmi Prabaningtyas Sri Rahayu Lestari Sueb Sugeng Handiyanto Sugi Hartono Suhadi Suhadi Sulisetijono Sulisetijono Sundari, Syifa Syafitri, Nur Laila Umi Lestari Umu Fatonatul Hidayah W. F. Edi Hanzen W.F Edi Hanzen Wa Ode Nurhawa Yahmi Ira Setyaningrum Yessi Hermawati Yesy Maulina Nadhifah Yheni Sapitri Yulia Venicreata Dipu Yulia Venicreata Dipu Yunita Putri Irsadul Ummah Yunita Rakhmawati Zahida, Nadila Sekar