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Kajian Kualitas Mikrobiologi Permen Labu Kuning berdasarkan Lama Penyimpanan dan Macam Pembungkus Ditinjau dari Angka Lempeng Total Koloni Kapang Linda Hapsari; Utami Sri Hastuti; Sitoresmi Prabaningtyas
Jurnal Ilmu Hayat Vol 2, No 2 (2018)
Publisher : Universitas Negeri Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17977/um061v2i22018p61-73

Abstract

Permen labu kuning adalah produk olahan buah labu kuning yang merupakan makanan khas dari Sumbawa Besar. Dalam permen labu kuning terkandung senyawa-senyawa yang dapat dimanfaatkan oleh kapang kontaminan untuk tumbuh dan berkembang biak. Hal ini dapat menyebabkan penurunan kualitas permen sehingga mempengaruhi daya tahan simpannya. Tujuan penelitian ini adalah: 1)Mengungkap pengaruh lama waktu penyimpanan, macam pembungkus, dan interaksi lama waktu penyimpanan dan macam pembungkus terhadap kualitas mikrobiologi permen labu berdasarkan ALT koloni kapang. 2) Menentukan batas waktu simpan maksimal permen labu ditinjau berdasarkan ALT koloni kapang yang masih memenuhi syarat batas maksimal dari DIRJEN POM. Jenis penelitian ini adalah eksperimen dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial yang terdiri dari dua faktor yaitu lama waktu penyimpanan dan macam pembungkus. Sampel permen labu kuning sebanyak 25 gram dihaluskan, kemudian dilarutkan dalam 225 ml larutan air pepton 0,1%, kemudian diencerkan sampai tingkat pengenceran10-6. Inokulasi pada medium lempeng Czapek Agar (CA) masing-masing 0,1 ml dari setiap tingkat pengenceran. Selanjutnya dilakukan penghitungan ALT koloni kapang baik pada sampel yang dibungkus kertas minyak maupun plastik yang telah disimpan selama 0 hari, 2 hari, 4 hari, 6 hari , dan 8 hari,  kemudian dirujukkan pada ketetapan dari DIRJEN POM. Hasil penelitian ini menunjukkan bahwa 1) Lama penyimpanan berpengaruh signifikan terhadap ALT koloni kapang, 2) Macam pembungkus dan interaksi antara lama penyimpanan dan macam pembungkus tidak berpengaruh terhadap ALT koloni kapang, 3)Batas waktu simpan pada permen labu kuning yang dibungkus kertas minyak maksimal adalah 4 hari sedangkan yang dibungkus plastik maksimal adalah 6 hari.
The Result of Second Field Test of Cellulose Degrading Bacteria from Mangrove Soil Microbiology Handout Nugraheni, Febriani Sarwendah Asri; Hastuti, Utami Sri; Rohman, Fatchur
Journal of Biological Science and Education Vol 2, No 1 (2020): June
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/jbse.v2i1.1227

Abstract

Learning media is one of the most important component in a learning process, both inside or outside a classroom. In order to provide proper learning for students based on the researcher’s prior research, at least, three different field tests are needed. This research is a quasi-experiment, which is provide result of the second field test of cellulose degrading bacteria from mangrove soil microbiology handout both in cognitive and psychomotor ability. The research aims are to describe effect of this microbiology handout on student’s cognitive achievement and gain data about inappropriate laboratory written instruction in this handout. Data gained by conducting learning activities using developed handout which were 2 times in class theory and three times of practical activity using instuctions written in the handout. In the end of class theory activity, students did a posttest to confirm their improvement of cognitive ability while their practical ability was observed using observation sheet along with their activities. Results showed that handout which being tested could improve student cognitive at average level (n gain 0,419). In contrast, several parts of laboratory written instruction need to be fixed to make better microbiology handout. 
Pengemasan dan pemasaran kopi biji salak (Salacca zalaca) melalui e-commerce untuk peningkatan perekonomian masyarakat desa Utomo, Muhammad Andry Prio; Rakhmawati, Yunita; Daniarsih, Ajeng; Hastuti, Utami Sri; Rozana, Kennis; Zahida, Nadila Sekar; Izzalqurny, Tomy Rizky
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 7 No 2 (2024)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v7i2.20872

Abstract

Tingginya produksi keripik salak Monik (Salacca zalacca) di desa Tirtoyudo juga diimbangi dengan meningkatnya jumlah limbah biji salak yang dihasilkan. Biji salak umumnya dibuang karena keras dan sulit untuk didegradasi sehingga mengakibatkan pencemaran lingkungan. Tujuan dari pengabdian kepada masyarakat ini adalah untuk memberikan pelatihan tentang pengemasan produk kopi biji salak Monik dengan easy drip dan pemasarannya melalui e-commerce kepada ibu-ibu PKK Desa Tirtoyudo, Malang. Metode yang digunakan dalam pelatihan ini yaitu service learning, sehingga peserta dapat mencoba praktik mengemas produk kopi biji salak dan mengelola tokodi e-commerce setelah materi. Jumlah peserta pada kegiatan pelatihan ini adalah 40 orang. Hasil pelatihan ini adalah produk box kopi biji salak kemasan easy drip dalam box yang menarik dan toko online di Shopee dengan nama Zacco Official Store. Shopee dipilih sebagai e-commerce untuk memasarkan produk karena 50% peserta pelatihan pernah melakukan transaksi di Shopee. Selain itu pelatihan ini mampu meningkatkan pengetahuan peserta tentang e-commerce, perkembangan e-commerce di Indonesia dan manfaat e-commerce menjadi 100%. Hasil survey pada akhir pelatihan menunjukkan peserta menilai puas hingga sangat puas untuk pelaksanaan pelatihan ini. Tindak lanjut yang diharapkan dari pengabdian ini adalah produk kopi biji salak Monik dapat teregistrasi di BPOM dan Halal MUI sehingga kualitas produk terjamin.
Development of Microbiology Module “Antibacterial Effect of Ginger Rhizome Extract to Inhibit the Growth of Propionibacterium acnes Bacteria” as a Teaching Material to Improve Students’ Problem Solving Skill Mahesti, Tika; Hastuti, Utami Sri; Sari, Murni Sapta
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.47971

Abstract

This research is a research and development of module on the topic "Antibacterial Effect of Ginger Rhizome Extract to Inhibit the Growth of P. acnes Bacteria" as a teaching material in the learning process to improve students' problem solving skills. The aim of developing the module is to produce a valid, practical and effective teaching materials. The development of module used ADDIE model. Validation of the Microbiology Module was conducted by Microbiology material expert, learning media and teaching material experts, and Microbiology learning practitioner expert. The effectiveness of Microbiology Module in improving problem solving skills can be known through pretest and posttest scores analyzed through One Way Ancova test. The research and development results show that validation scores by learning media and teaching materials experts were 99.72%, Microbiology material experts were 87.56%, biology education practitioner experts were 99.33%, student practical response results were 87.05%. The efficiency results were 0.00 < 0.05 so it proved that the use of module is effective in the learning process, it can be concluded that the Microbiology Module category as very valid, very practical and effective as teaching material, so it can be used by students in Microbiology courses especially in improving problem solving skills.
A Microbiology Module Development on Antagonism Between Fungi and Bacteria Based on Problem-Based Learning to Improve Student’s Critical Thinking Skills Maknuna, Durrotul; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.50920

Abstract

The purpose of the research is to develop Microbiology Module on the topic "The Antagonism of P. chrysogenum against Escherichia coli, Staphylococcus aureus, and Bacillus subtilis" that have criteria valid, practice, and effective for improve the student’s critical thinking skills. This research type is a quasi-experiment research using a non-randomized pretest-posttest control group design. The learning model uses the ADDIE approach which is implemented with the Problem Based Learning (PBL) model. Data collection uses response questionnaires, pretest and post-test, and validation sheets. The effectiveness of the Microbiology Module in improving students' critical thinking skills can be known through the pretest and post-test scores analyzed through the One Way Ancova test. The research and development result proved that the validation score gives by media and teaching materials experts was 99%, material experts were 98%, and Biology education practitioners were 100% from the student responses were 93%. The p-level results showed 0.00<0.05, so the modules were effective to use in the learning process. The research results proved that a significant difference between the experimental class learned using the Microbiology Module compared to the control class PowerPoint without the Microbiology Module. The modules is valid, practical, and effective to use in improve the student’s critical thinking skills.
Development of Microbiology Handout as Teaching Material to Improve Science Process Skills of Health Vocational Students Pratama, Aris Yudha; Hastuti, Utami Sri; Setiowati, Frida Kunti
BIOEDUKASI Vol 22 No. 3 (2024)
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v22i3.49473

Abstract

This research is a research and development of Microbiology Handout on the topic "Antibacterial Test on Bacterial Growth Inhibition" which uses natural materials, namely star fruit extract (Averrhoa carambola L.), which will be used as teaching materials in the learning process to help improve students' science process skills. The purpose of developing Microbiology Handout is to produce teaching materials that are valid, practical and effective for use in learning Microbiology subjects. Microbiology Handout development uses the ADDIE development model. Validation of Microbiology Handouts was carried out by learning media and teaching material expert validators, Microbiology material experts, and Microbiology learning practitioner expert validators. The Microbiology Handout trial was conducted through three stages of trials, namely a one-to-one trial of 3 students, a small group trial of 10 students, and a field trial of 15 students. The effectiveness of Microbiology Handout in improving students' science process skills can be seen through pretest and posttest scores analyzed using the IBM SPSS version 26 application through the One Way Ancova test. The results of research and development show the acquisition of validation scores by learning media and teaching material experts of 100%, Microbiology material experts of 94.3%, education practitioner experts of 99.64%, student response results of 91.43% and efficiency results of 0.028
Microbiology literacy and its influence on knowledge, perceptions and community attitudes Mustofa, Ali; Hastuti, Utami Sri; Susanto, Hendra
Biosfer: Jurnal Pendidikan Biologi Vol. 18 No. 1 (2025): Biosfer: Jurnal Pendidikan Biologi
Publisher : Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/biosferjpb.49273

Abstract

Microbiology literacy development shapes people's attitudes, behaviors, and perceptions toward microorganisms. This study aims to explore the relationship between microbiological literacy and people's perceptions and attitudes towards microorganisms. This study uses the Systematic Literature Review (SLR) method to analyze the relationship between microbiological literacy and public knowledge, attitudes, and perceptions of microorganisms. Data were retrieved from Scopus using relevant keywords and then filtered based on the following criteria: published between 2019–2024, in English, and open access. The analysis was conducted through four steps: coding, concept review, and summarizing key information (author, year, and core concepts). The results were synthesized to map the relationship between microbiological literacy and public perceptions. The results showed that respondents' knowledge levels varied about various health issues. Good microbiological knowledge can improve health literacy, affecting individual attitudes and perceptions of health. Regarding respondents' attitudes, it is known that respondents are interested in education and health awareness. A positive attitude toward health can encourage actions that support public health. In contrast, individuals with low microbiology knowledge orientation are more likely to cause negative perceptions and distrust of health interventions. Continuous, evidence-based education is essential to improve microbiology and health literacy and shape positive attitudes toward health interventions. Microbiology knowledge has a broad impact on daily life and the future. However, there are significant challenges in understanding microbiology. Public perception of microbes is often only associated with 'germs' and negative images. Therefore, microbiology knowledge in learning must be presented in visual arts and visual, olfactory, and tactile experiences. The more we understand the role and knowledge of microbes, the better we can make relevant decisions.
Development of Guided Inquiry-Based Microbiology Modules Based on the Identification of Contaminant Molds in Tomato Fruit to Improve Students' Science Process Skills Naufal Wima Al Fahri; Utami Sri Hastuti; Safwatun Nida
BIOEDUKASI: Jurnal Biologi dan Pembelajarannya Vol. 23 No. 3 (2025): Vol. 23, No. 3, October 2025
Publisher : PROGRAM STUDI PENDIDIKAN BIOLOGI FAKULTAS KEGURUAN DAN ILMU PENDIDIKAN UNIVERSITAS JEMBER

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19184/bioedu.v23i3.53749

Abstract

Students often experience difficulties in identifying microbiological specimens due to limitations in preparing and interpreting microscopic slides. This problem is evident in the context of tomato fruits (Solanum lycopersicum), which are widely consumed agricultural commodities but are highly perishable and susceptible to microbial contamination, especially by molds. To address this challenge, this study aims to develop a guided inquiry-based microbiology learning module using tomato mold identification as a case study, with the goal of improving students’ Science Process Skills (SPS). The research was conducted in two stages: (1) laboratory experiments to identify contaminant molds in tomatoes and (2) development of a learning module using the ADDIE model (Analyze, Design, Develop, Implement, Evaluate). The sample consisted of 62 microbiology students from the Biology Education Program, Faculty of Mathematics and Natural Sciences, State University of Malang. A non-randomized control-group pretest–posttest design was employed, the increase in posttest results compared to the pretest was calculated using the N-gain score. Results showed that students’ SPS was still relatively low, particularly in the observing indicators (30.43%) and hypothesizing indicators (43.48%). The developed module integrates real laboratory findings into structured inquiry-based activities, emphasizing hands-on practice, critical thinking, and contextual relevance to agricultural microbiology. Its distinctive features include the use of authentic microbial identification data, scaffolding through guided inquiry steps, and alignment with SPS indicators. This module is expected to provide an effective solution for enhancing students’ SPS in microbiology learning
Duration Effects of Shrimp Paste Storage on ALT of Mold Colonies: Variations by Brand and Pre-Standard Treatment Syafitri, Nur Laila; Hastuti, Utami Sri; Prabaningtyas, Sitoresmi
JURNAL PEMBELAJARAN DAN BIOLOGI NUKLEUS Vol 9, No 3: Jurnal Pembelajaran Dan Biologi Nukleus November 2023
Publisher : Universitas Labuhanbatu

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36987/jpbn.v9i3.4690

Abstract

Shrimp paste is a commonly employed condiment or flavoring in culinary preparations. As a result of the nutrients present in shrimp paste, fungi and other microbial contaminants are able to proliferate and develop on the paste. This study aims as follows: 1) to determine ALT measurements of mold colonies to assess the quality of two brands of pre-treated shrimp paste; and 2) to identify contaminating mold species present in steamed and unsteamed shrimp paste prior to storage. Descriptive quantitative and qualitative research methods were employed within the Biology Department of FMIPA UM's Microbiology Laboratory. A series of three samplings were conducted on treated shrimp paste brands A and B, one week apart between each sampling. A volume of 90 ml of 0.1% peptone water was used to dissolve 10 grams of shrimp paste, resulting in a dilution of 10-1; this process was repeated until the dilution reached 10-5. 0. The surface of PDA (Potato Dextrose Agar) medium was inoculated with 1 ml of suspension from each dilution. The medium was subsequently incubated at a temperature of 25oC for 7x24 hours. The findings of this study indicate the following: 1) At the 28th day of storage, both brands of shrimp paste that underwent treatment with steamed and unsteamed shrimp paste remained of acceptable quality for human consumption. 2) In steamed and unsteamed shrimp paste, thirteen species of mold contaminants were identified: Chrysosporium corda, Cladosporium sphaerospermum, Penicillium frequentans, Penicillium chrysogenum, Rhizoctonia sp1., Aspergillus candidus, Fusarium equeseti, Colletotrichum ti, Moniliella acetobutens, and Rhizoctonia sp3
Pemanfaatan limbah klobot salak monik sebagai nata-mocca untuk peningkatan ekonomi desa Utomo, Muhammad Andry Prio; Rakhmawati, Yunita; Daniarsih, Ajeng; Hastuti, Utami Sri; Lestari, Sri Rahayu; Izzalqurny, Tomy Rizky
Jurnal Inovasi Hasil Pengabdian Masyarakat (JIPEMAS) Vol 8 No 3 (2025)
Publisher : University of Islam Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33474/jipemas.v8i3.22746

Abstract

Kegiatan pengabdian kepada masyarakat ini bertujuan untuk memberikan keterampilan dalam pembuatan nata menggunakan klobot salak Monik serta branding dan pemasarannya via online. Metode pelaksanaan pelatihan ini adalah learning service dengan pendekatan demonstrasi. Rangkaian acara pelatihan meliputi visitasi dan koordinasi dengan industri keripik salak, simulasi pembuatan nata salak Monik oleh tim, pelatihan kepada peserta, pengisian pre-test dan post-test, dan evaluasi pelatihan. Pelatihan ini diikuti oleh 20 peserta yang merupakan anggota PKK desa Tirtoyudo yang sebagian besar bekerja sebagai karyawati di pabrik industri keripik salak. Pelatihan menghasilkan 20 toples Nata-Mocca yang merupakan nata yang media fermentasinya menggunakan ekstrak limbah salak Monik. Produk Nata-Mocca tersebut telah dikemas secara menarik daa dipasarkan secara online melalui e-commerce Shopee. Berdasarkan hasil pengisian pre-test dan post-test yang dianalisis menggunakan T-test (p>0,05) menunjukkan hasil yang berbeda signifikan sehingga pelatihan efektif dalam meningkatkan pengetahuan dan keterampilan dalam pembuatan nata. Hasil evaluasi pelatihan menunjukkan semua indikator dinilai sangat baik oleh 80% peserta. Melalui pelatihan ini diharapkan produk Nata-Mocca olahan limbah klobot dapat dijadikan alternatif untuk peningkatan ekonomi warga desa Tirtoyudo.
Co-Authors *Ali Mustofa Abdul Fattah Noor Abdul Ghofur Abdul Gofur Abdul Gofur Abdul Gofur Abdul Gofur Ade Wahyu Pratama Agung Witjoro Ahmad Najib Ajeng Daniarsih Ana Syarifatun Nisa Anggia Oktantia Anindya Nirmala Permata Arif Rahman Arini Zahrotun Arini Zahrotun Nasichah Ary Maf’ula Asna, Putri Moortiyani Al Aswal Salewangeng Aziz Tanama Balqis Balqis Betty Lukiati Chandra Kirana Chandra Kirana Chandra Kirana Choirunnisa, Hesti Nur Chomisatut Thoyibah Dina Istia’nah Dwi Listyorini Dwi Rahmawati Dwi Rahmawati Dwi Rakhmawati Dwi Rakhmawati Endang Suarsini Erna Wijayanti Faiza Nur Imawati Ningsih Fatchur Rohman Fatchur Rohman Fatchur Rohman Febriani Sarwendah Asri Nugraheni Febriani Sarwendah Asri Nugraheni Fitri, Rizka Diah Fitria Maulita Fitria Maulita Fitriyah, Rofiqoh Lailatul Frida Kunti Setiowati Galina Istiqhfarini Gani, Abdul Rasyid Fakhrun Hadi Suwono Hanzen, W.F Edi Hendra Susanto Henny Nurul Khasanah Henny Nurul Khasanah Henny Nurul Khasanah Herawati Susilo Herdina Rahma Zalita Hesti Nur Choirunnisa Ibrohim Imam Mudakir Indah Sari Dewi Indriana Rahmawati Indriana Rahmawati Indriana Rahmawati Intan Rezki Kurniasari Istamar Syamsuri Izzalqurny, Tomy Rizky Izzatinnisa’ Izzatinnisa’ Khusnul Khotimah Kuniasari, Intan Rezki La Arlan Lely Hermawati Linda Hapsari Linda Hapsari Luluk Rofiatul Mafluhah M. Ainur Yaqin M. Amin M. Nidhamul Maulana Mahesti, Tika Maknuna, Durrotul Mareta Arisswara Edy Mariyanti Mariyanti Mashuri Saputra Mastika, Laily Maghfiro Kamil Maya Firdausi Prayudhani Muhammad Andry Prio Utomo Muhammad Syamsussabri Murni Sapta Sari Natalia Rosa Keliat Naufal Wima Al Fahri Ningsih, Husdiani Nugraheni, Febriani Sarwendah Asri Nursyahbani Saraha Nurul Yanuarsih Nuzalifa, Yossie Ulfa Otavia Dewi Permata Ika Hidayati Pratama, Aris Yudha Putri M. Al Asna Putri Moortiyani Al Asna Qorry Aulya Rohmana Rahel Natalia Saragih Munthe Rahmadyah Kusuma Putri Ria Yustika Sari Ria Yustika Sari Ria Yustika Sari Rina Kristina Maria Rizky, Mirza Yanuar Rofiqoh Lailatul Fitriyah Rozana, Kennis Safrudin M. Abidin Saidil Mursali Saidil Mursali Sari, Ria Yustika Siti Aisaroh Siti Annisaa&#039;ul Kariimah Siti Hartina Pratiwi Siti Zubaidah Siti Zubaidah Sitoresmi Prabaningtyas Sri Rahayu Lestari Sueb Sugeng Handiyanto Sugi Hartono Suhadi Suhadi Sulisetijono Sulisetijono Sundari, Syifa Syafitri, Nur Laila Tamalia Tama Umi Lestari Umu Fatonatul Hidayah W. F. Edi Hanzen W.F Edi Hanzen Wa Ode Nurhawa Yahmi Ira Setyaningrum Yessi Hermawati Yesy Maulina Nadhifah Yheni Sapitri Yulia Venicreata Dipu Yulia Venicreata Dipu Yunita Putri Irsadul Ummah Yunita Rakhmawati Zahida, Nadila Sekar