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Heri Hermansyah
Chemical Engineering Department, Engineering Faculty, Universitas Indonesia, Depok 16424, Indonesia
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Journal : Barometer

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BIODELIGNIFICATION OF AGRO-INDUSTRIAL WASTE AS THE SUBSTRATE TO PRODUCE LACCASE ENZYME FROM TRAMETES VERSICOLOR Perdani, Meka Saima; Malisi, Sibro; Putri, Dwini Normayulisa; Margaretha, Grace; Hermansyah, Heri
BAROMETER Vol 9 No 1 (2024): Barometer
Publisher : Fakultas Teknik, Universitas Singaperbangsa Karawang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35261/barometer.v9i1.11018

Abstract

Agro-industrial waste in Indonesia is a significant problem for the environment. Optimization of agro-industrial waste utilization is an exciting thing to be researched further. This study aims to utilize agro-industrial waste to optimize laccase enzyme production. The agro-industrial waste used is in the form of bagasse, rice straw, and corn stalks. The waste is used as biomass and treated to extract cellulose through bio-delignification. Cellulose serves as a substrate substance in the production of laccase enzymes. Laccase enzymes can be applied as a biocatalyst in the environmental, pharmaceutical, and chemical industries. Cellulose from agroindustry waste was synthesized by heat treatment using steam explosion with the operation conditions 200°C and 10 bar for 10 minutes. The production of laccase enzymes was done using the green technology submerged fermentation method for 32 hours at 25°C. The optimal result in producing laccase enzyme is using substrate from corn stalks and adding nutrients with an activity value of 25.46 U/mL. The reaction kinetics of laccase enzyme and commercial enzyme against 0.1 M substrate were modelled using the Euler method, and the k value was 2x10-4 min-1 (crude laccase) and 1.9x10-2 min-1 (commercial laccase).
Co-Authors ., Nuryetti Abdal Rohim Abdurrohman Adinda Putri Wisman, Adinda Agung Sri Hendarsa Agung Sri Hendarsa, Agung Sri Anandho Wijanarko ANGGI KHAIRINA HANUM HASIBUAN Annisa Kurnia, Annisa Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Arif Rakhman Suharso Azis Setiawan Bambang Prasetya Bambang Prasetya Cahya Eko Susilo Catur Nitya V.N. Catur Nitya V.N., Catur Nitya Chulasiri, Malyn Cindy Rianti Priadi Dadan Jaelani deden rosid waltam Deni Novitasari Deni Novitasari, Deni Desti Andani Dianursanti Dienayati, Dara Diki Firdaus, Diki Eki Listya Rini Ester Kristin Ester Kristin, Ester Fajar Achmadi Fajar Achmadi Prianto Firda Firda Hamdi, Darul Hanif Yuliani Hanung Adi Nugroho I Nyoman Adi Putra Ira Setiawati Ira Trisnawati Ira Trisnawati, Ira Jessica Tanuwijaya Jessica Tanuwijaya, Jessica Josia Simanjuntak Josia Simanjuntak Josia Simanjuntak Julianty, Liska Kitakawa, Naomi Shibasaki Kusuma Devi, Pitria M Baiquni M Baiquni M Baiquni M Samsuri M Samsuri, M M. Baiquni Maemonah, Maemonah Malisi, Sibro Mardias, R. Margaretha, Grace Meliana R Misri Gozan Mohamad Nasikin Mohammad Nasikin Mohammad Nasikin Momoji Kubo Momoji Kubo, Momoji Muhammad Yahya Muhaimin Muhammad Yahya Muhaimin Mulyati, Lia Muryanto Muryanto Muryanto Muryanto, Muryanto Nanang Saprudin Naomi Shibasaki-Kitakawa Naomi Shibasaki-Kitakawa, Naomi Nasikin, M. Nasikin, Muhammad Nurlaila Nurlaila Nurmalasari Nurmalasari Perdani, Meka Saima Pranatha, Aria Praswasti P. D.K Wulan Praswasti P. D.K Wulan, Praswasti P. D.K Praswasti Pembangun Dyah Kencana Wulan Praswasti Wulan Praswasti Wulan, Praswasti Prianto, Fajar Achmadi Putri, Dwini Normayulisa R Mardias R Mardias, R Rahayuningrat, Nurunnisa Rahmawati, Fadiah Ramadhani, Cecep Rauntu, Astryanike Rita Arbianti Rita Arbiyanti Roekmijati Soemantojo Roekmijati Soemantojo, Roekmijati Roekmijati W. Soemantojo Roekmijati W. Soemantojo, Roekmijati W. Rudi Hartono Rudi Hartono Rusdi Rusdi Rusdi Rusdi SAHLAN, MUHAMAD Samsuri, M. Septian Marno Setiawan, Azis Siswa Setyahadi Slamet . Slamet Slamet Soemantojo, Roekmijati Widaningroem Sukmanawati, Dera Tani Surya Utami Tania S Utami Tania Surya Utami Tania Utami Tania Utami, Tania Teguh Pambudi Tepi Muhammad Romdoni Toshiy Yonemoto Toshiy Yonemoto, Toshiy Ujang Suherman Wijanarko, A. Wiwik Handayani Wulandari, Afni Yana Hendriana Yana Hendriana Yuliusman Yuliusman Yunisa Fahmi, Nadya Zahra, Soraya Zakaria, Ricky Muhamad