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Journal : Makara Journal of Technology

Utilization of Bagasse Cellulose for Ethanol Production through Simultaneous Saccharification and Fermentation by Xylanase Samsuri, M.; Gozan, Misri; Mardias, R.; Baiquni, M.; Hermansyah, Heri; Wijanarko, Anondho; Prasetya, Bambang; Nasikin, M.
Makara Journal of Technology Vol. 11, No. 1
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Utilization of Bagasse Cellulose for Ethanol Production through Simultaneous Saccharification and Fermentation by Xylanase. Bagasse is a solid residue from sugar cane process, which is not many use it for some product which have more added value. Bagasse, which is a lignosellulosic material, be able to be use for alternative energy resources like bioethanol or biogas. With renewable energy resources a crisis of energy in Republic of Indonesia could be solved, especially in oil and gas. This research has done the conversion of bagasse to bioethanol with xylanase enzyme. The result show that bagasse contains of 52,7% cellulose, 20% hemicelluloses, and 24,2% lignin. Xylanase enzyme and Saccharomyces cerevisiae was used to hydrolyse and fermentation in SSF process. Variation in this research use pH (4, 4,5, and 5), for increasing ethanol quantity, SSF process was done by added chloride acid (HCl) with concentration 0.5% and 1% (v/v) and also pre-treatment with white rot fungi such as Lentinus edodes (L.edodes) as long 4 weeks. The SSF process was done with 24, 48, 72, and 96 hour’s incubation time for fermentation. Variation of pH 4, 4,5, and 5 can produce ethanol with concentrations 2,357 g/L, 2,451 g/L, 2,709 g/L. The added chloride acid (HCl) with concentration 0.5% and 1% (v/v) and L. edodes can increase ethanol yield, The highest ethanol concentration with added chloride acid (HCl) concentration 0.5% and 1% consecutively is 2,967 g/L, 3,249 g/L. The highest ethanol concentration with pre-treatment by L. edodes is 3,202 g/L.
Kinetic Model For Triglyceride Hydrolysis Using Lipase:Review Hermansyah, Heri; Wijanarko, A.; Dianursanti, Dianursanti; Gozan, Misri; Wulan, Praswasti P. D.K; Arbianti, Rita; Soemantojo, Roekmijati W.; Utami, Tania Surya; Yuliusman, Yuliusman; Kubo, Momoji; Kitakawa, Naomi Shibasaki; Yonemoto, Toshiy
Makara Journal of Technology Vol. 11, No. 1
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Triglyceride hydrolysis using lipase has been proposed as a novel method to produce raw materials in food and cosmetic industries such as diacylglycerol, monoacylglycerol, glycerol and fatty acid. In order to design a reactor for utilizing this reaction on industrial scale, constructing a kinetic model is important. Since the substrates are oil and water, the hydrolysis takes place at oil-water interface. Furthermore, the triglyceride has three ester bonds, so that the hydrolysis stepwise proceeds. Thus, the reaction mechanism is very complicated. The difference between the interfacial and bulk concentrations of the enzyme, substrates and products, and the interfacial enzymatic reaction mechanism should be considered in the model.
Biomass Production Chlorella Vulgaris Buitenzorg Using Series of Bubble Column Photo Bioreactor with a Periodic Illumination Wijanarko, Anondho; Dianursanti, Dianursanti; Muryanto, Muryanto; Simanjuntak, Josia; Kencana Wulan, Praswasti Pembangun Dyah; Hermansyah, Heri; Gozan, Misri; Soemantojo, Roekmijati Widaningroem
Makara Journal of Technology Vol. 12, No. 1
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Chlorella vulgaris Buitenzorg cultivation using three bubble column photo bioreactors arranged in series with a volume of 200 mL for 130 hours shows an increase of biomass production of Chlorella vulgaris Buitenzorg up to 1.20 times and a decrease of the ability of CO2 fixation compared to single reactor at a periodic sun illumination cycle. The operation conditions on cultivation are as following: T, 29.0oC; P,1 atm.; UG, 2.40 m/h; CO2, 10%; Benneck medium; and illumination source by Phillip Halogen Lamp 20W /12V/ 50Hz. Other research parameters such as microbial carbon dioxide transferred rate (qco2), CO2 transferred rate (CTR), energy consumption for cellular formation (Ex), and cultural bicarbonate species concentration [HCO3] also give better results on series of reactor.
Performance Optimization of Microbial Fuel Cell (MFC) Using Lactobacillus bulgaricus Arbianti, Rita; Utami, Tania Surya; Hermansyah, Heri; Novitasari, Deni; Kristin, Ester; Trisnawati, Ira
Makara Journal of Technology Vol. 17, No. 1
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Electrical energy needs in Indonesia are expected to continue to rise. The use of petroleum as a source of energy still dominates, although oil reserves in Indonesia are increasingly being depleted. Therefore, there is a need to develop alternative sources of sustainable energy, such as microbial fuel cell (MFC). In this study, Lactobacillus bulgaricus was used as an electricity producer in a dual-chamber MFC reactor. We investigated the maximum electrical energy by varying the bacterial optical density (OD), the operational time of MFC, the reactor volume, the electrolyte solution, and the configuration of MFC reactor. In this study, the maximum electrical energy (201.8 mW/m2) was generated at an OD of 0.5 in an MFC reactor series using potassium permanganate as the electrolyte solution.
Encapsulation Process of Propolis Extract by Casein Micelle Improves Sunscreen Activity Sahlan, Muhamad; Dienayati, Dara; Hamdi, Darul; Zahra, Soraya; Hermansyah, Heri; Chulasiri, Malyn
Makara Journal of Technology Vol. 21, No. 1
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Propolis, a wax bee glue, have antioxidant activity and effective as broad spectrum UVB and UVA photoprotection sunscreens agent. To improve photoprotection activity in sunscreen cream, the propolis was encapsulated by casein micelle. The Indonesian propolis was extracted by ethanol and separated the wax by freeze precipitation. The extract was encapsulated by casein and reduced their size by ball mill homogenizer with the encapsulation efficiency about 80% and size of particles about 80 nm. The particles was applied in sunscreen cream, evaluate their stabillity and photoprotection activity Sun Protection Factor (SPF) spectrophotometer (Optometric 290s). The results shows that the selected creams were stable and have moderate photoprotection activities higher than the controls. The encapsulation process of propolis by casein micelle can improve their photoprotection activity.
Determination of the Kinetic Parameters of Cholesterol Oxidation using Cholesterol Oxidase from Streptomyces sp. Perdani, Meka Saima; Hermansyah, Heri; Sahlan, Muhamad; Putri, Dwini Normayulisa; Pambudi, Teguh; Hasibuan, Anggi Khairina Hanum
Makara Journal of Technology Vol. 28, No. 3
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Cholesterol oxidase (CO) was successfully produced from Streptomyces sp. via the submerged fermentation method, and 69 U/mL enzyme activity was obtained. This study aimed to determine cholesterol oxidation kinetics and the production of CO as a catalyst. The enzyme was diluted to 0.15, 0.075, and 0.00375 U/mL for the oxidation reaction. The substrate was also prepared in three concentrations: 3.23, 6.46, and 12.93 mM. The optimization of conditions for enzymatic cholesterol oxidation was investigated through measurement of the effect of initial cholesterol and enzyme concentrations. Cholesterol concentration was rapidly measured via high-performance liquid chromatography (HPLC). The kinetics of CO were modeled using the first-order irreversible reaction. An enzymatic kinetic model was derived, and it was verified using experimental data and sensitivity analysis. Based on the experiment, the highest enzyme concentrations of crude and commercial CO can oxidize the substrate up to 84% within 240 min. However, the oxidation reaction showed a slightly different behavior in the early 60 min, and crude CO exhibited a slower substrate oxidation. The kinetic rate constant obtained by Euler’s method reached 1.0 x 10−3/min and 1.41 x 10−3/min for 0.15 U/mL crude and commercial CO, respectively.
Co-Authors ., Nuryetti Abdal Rohim Abdurrohman Ade Saprudin Adinda Putri Wisman, Adinda Agung Sri Hendarsa Agung Sri Hendarsa, Agung Sri Anandho Wijanarko ANGGI KHAIRINA HANUM HASIBUAN Annisa Kurnia, Annisa Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Anondho Wijanarko Arif Rakhman Suharso Azis Setiawan Bambang Prasetya Bambang Prasetya Cahya Eko Susilo Catur Nitya V.N. Catur Nitya V.N., Catur Nitya Chulasiri, Malyn Cindy Rianti Priadi Dadan Jaelani deden rosid waltam Deni Novitasari Deni Novitasari, Deni Desti Andani Dianursanti Dienayati, Dara Diki Firdaus, Diki Eki Listya Rini Ester Kristin Ester Kristin, Ester Fajar Achmadi Fajar Achmadi Prianto Firda Firda Hamdi, Darul Hanif Yuliani Hanung Adi Nugroho I Nyoman Adi Putra Ika Kurniaty Ira Setiawati Ira Trisnawati Ira Trisnawati, Ira Jessica Tanuwijaya Jessica Tanuwijaya, Jessica Josia Simanjuntak Josia Simanjuntak Josia Simanjuntak Julianty, Liska Kitakawa, Naomi Shibasaki Kusuma Devi, Pitria M Baiquni M Baiquni M Baiquni M Samsuri M Samsuri, M M. Baiquni Maemonah, Maemonah Malisi, Sibro Mardias, R. Margaretha, Grace Meliana R Misri Gozan Mohamad Nasikin Mohammad Nasikin Mohammad Nasikin Momoji Kubo Momoji Kubo, Momoji Muhammad Yahya Muhaimin Muhammad Yahya Muhaimin Mulyati, Lia Muryanto Muryanto Muryanto Muryanto, Muryanto Nanang Saprudin Naomi Shibasaki-Kitakawa Naomi Shibasaki-Kitakawa, Naomi Nasikin, M. Nasikin, Muhammad Nurislami, Gina Fauziah Nurlaila Nurlaila Nurmalasari Nurmalasari Perdani, Meka Saima Pranatha, Aria Praswasti P. D.K Wulan Praswasti P. D.K Wulan, Praswasti P. D.K Praswasti Pembangun Dyah Kencana Wulan Praswasti Wulan Praswasti Wulan, Praswasti Prianto, Fajar Achmadi Putri, Dwini Normayulisa R Mardias R Mardias, R Rahayuningrat, Nurunnisa Rahmawati, Fadiah Ramadhani, Cecep Rauntu, Astryanike Rita Arbianti Rita Arbiyanti Roekmijati Soemantojo Roekmijati Soemantojo, Roekmijati Roekmijati W. Soemantojo Roekmijati W. Soemantojo, Roekmijati W. Rudi Hartono Rudi Hartono Rusdi Rusdi Rusdi Rusdi SAHLAN, MUHAMAD Samsuri, M. Septian Marno Setiawan, Azis Siswa Setyahadi Slamet . Slamet Slamet Soemantojo, Roekmijati Widaningroem Sukmanawati, Dera Tani Surya Utami Tania S Utami Tania Surya Utami Tania Utami Tania Utami, Tania Teguh Pambudi Tepi Muhammad Romdoni Toshiy Yonemoto Toshiy Yonemoto, Toshiy Ujang Suherman Wijanarko, A. Wiwik Handayani Wulandari, Afni Yana Hendriana Yana Hendriana Yuliusman Yuliusman Yunisa Fahmi, Nadya Zahra, Soraya Zakaria, Ricky Muhamad