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Suplementasi Enzim Papain dalam Pakan terhadap Performa Pertumbuhan, Efisiensi Pemanfaatan Pakan dan Kelulushidupan Udang Vaname (Litopenaeus vannamei) Rachmawati, Diana; Hutabarat, Johannes; Dewi, Eko Nurcahya; Windarto, Seto
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (427.483 KB) | DOI: 10.14710/jmr.v9i3.27609

Abstract

Udang vaname (Litopenaeus vannamei) adalah karnivora yang sukar mencerna protein nabati dalam pakan sehingga efisiensi pemanfaatan pakan belum maksimal.  Hampir 40-60% dari total biaya produksi hanya untuk biaya pakan. Efisiensi pemanfaatan pakan dapat ditingkatkan dengan penambahan enzim papain dalam pakan yang mampu meningkatkan penguraian dan pencernaan bahan pakan yang mengandung protein nabati sederhana sehingga dapat meningkatkan daya cerna protein pakan. Penelitian ini bertujuan mengkaji pengaruh suplementasi enzim papain dalam pakan terhadap efisiensi pemanfaatan pakan, performa pertumbuhan dan kelulushidupan udang vaname. Hewan uji berupa udang vaname dengan bobot rata-rata 3,02±0,21 g/ekor yang diperoleh dari pembudidaya udang vaname Desa Blebak, Kecamatan Mlonggo, Jepara.  Metode dalam penelitian ini adalah metode eksperimen, dengan Rancangan Acak Lengkap, 4 Perlakuan dan 3 kali pengulangan. Terdapat 4 perlakuan suplementasi enzim papain dalam pakan yaitu A (0 %), B (0,1 %), C (0,2 %), dan D (0,3 %). Parameter yang diamati meliputi EPP, PER, RGR, SR dan parameter kualitas air. Hasil penelitian menunjukkan bahwa penambahan enzim papain dalam pakan meningkatkan EPP, PER, RGR, namun tidak berpengaruh pada SR udang vaname. Enzim papain sebesar 0,1%/kg pakan merupakan dosis optimal pada EPP, PER dan RGR menghasilkan nilai maksimal sebesar 66,51%, 1,64 dan 4,22%/hari. White shrimp (Litopenaeus vannamei) is a carnivorous animal that difficult to digests vegetable protein in the feed so that the efficiency of feed utilization is not maximal. Nearly 40-60% of the total production cost is only for feed cost. Feed efficiency is improved by using papain in the feed which can increase the breakdown and consumption of feed containing vegetable protein so that it can easily increase the digestibility of feed protein. This study examines the effect of papain supplementation in feed on the growth performance, efficiency of feed utilization, and survival rate of white shrimps. The test animals were white shrimp with an average weight of 3.02 ± 0.21 g / individuals obtained from the whiteleg shrimp farmers in Blebak Village, Mlonggo District, Jepara. The method in this study was an experimental method, with a completely randomized design, 4 treatments, and 3 repetitions. There were 4 supplements of papain in feed: A (0%), B (0.1%), C (0.2%), and D (0.3%). The parameters were EPP, PER, RGR, SR, and water quality parameters. The results showed that the supplementation of the papain in feed increased the EPP, PER, RGR, but had no effect on the SR of white shrimp. Papain of 0.1% / kg of feed was the optimal dose at EPP, PER, and RGR resulting in a maximum value of 66.51%, 1.64 and 4.22% / day. 
Pengaruh Pemberian Pakan Phronima sp. Substitusi Artemia sp. Terhadap Pertumbuhan dan Kelulushidupan Udang Vaname (Litopenaeus vannamei) Ratri, Kartika Sulistyaning; Hutabarat, Johannes; Herawati, Vivi Endar
Jurnal Masyarakat Akuakultur Indonesia Vol 3, No 2 (2019): Volume 3 (2) 2020
Publisher : Masyarakat Akuakultur Indonesia

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Abstract

Udang vaname (Litopenaeus vannamei) merupakan salah satu komoditas yang paling baik untuk dikembangkan pada kegiatan budidaya. Salah satu penunjang keberhasilan budidaya yaitu ketersediaan pakan alami yang berkualitas. Phronima sp. merupakan alternatif pengganti pakan alami Artemia sp.Tujuan penelitian adalah mengetahui pengaruh substitusi Phronima sp. dengan Artemia sp. terhadap laju pertumbuhan dan kelulushidupan udang vaname (L. vannamei) serta untuk mengetahui dosis substitusi terbaik.Hewan uji yang digunakan adalah 450 ekor udang vaname dengan bobot rata-rata 0,029±0,001 gr/ekor dipelihara dengan padat tebar 27 ekor dengan volume air 6 liter. Masa pemeliharaan selama enambelas hari.Rancangan percobaan yang digunakan adalah rancangan acak lengkap (RAL) 5 perlakuan dan 3 ulangan. Dosis yang digunakan yaitu perlakuan A (100% Artemia sp.), B (75% Artemia sp. dan 25% Phronima sp.), C (50% Artemia sp. dan 50% Phronima sp.), D (25% Artemia sp. dan 75% Phronima sp.), dan E (100% Phronima sp.). Pemberian pakan dilakukan sebanyak 3kali sehari yaitu pagi (07.00 WIB), siang hari (13.00 WIB) dan sore hari (19.00 WIB). Data yang diamati adalah Laju Pertumbuhan Relatif (RGR), Pertumbuhan Panjang Mutlak, Bobot Biomass, Laju Pemanfaatan Pakan Alami (Grazing rate), Kelulushidupan (SR), dan Kualtas Air.Hasil penelitian menunjukkan bahwa dengan kombinasi dosis 75% Artemia sp. dan 25% Phronima sp. (B) menghasilkan nilai tertinggi pada RGR sebesar 0,350±0,001%; pertumbuhan panjang mutlak 1,77±0,06cm; bobot biomass 0,0101±0,00004; kelulushidupan 0,95±0,06%. Kombinasi dosis 100% Artemia sp. (A) menghasilkan nilai tertinggi pada grazing rate sebesar 21,84±0,04ind/hari. Kualitas air selama masa pemeliharaan sudah optimal dengan nilai kualitas air pada suhu berkisar antara 28-30,20C; pH 8; oksigen terlarut (DO) 4,26-5,38 mg/l; salinitas 25-28 ppt.
Nutritional Content of Artemia sp. Fed with Chaetoceros calcitrans and Skeletonema costatum VIVI ENDAR HERAWATI; JOHANNES HUTABARAT; OCKY KARNA RADJASA
HAYATI Journal of Biosciences Vol. 21 No. 4 (2014): December 2014
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1231.645 KB) | DOI: 10.4308/hjb.21.4.166

Abstract

Artemia sp. is a natural food with high protein content, especially amino acid. Nowadays, Indonesia still relies on import for its supply. Hence, the utilization of local Artemia sp. as an alternative to the imported product is recommended as it contains more protein and less expensive. The advantages of local Artemia sp. is its better crystal quality as it is still fresh. It also provides better income for salt farmers because waste water from salt farm can be used to culture Artemia sp.. This research is aimed at determining the quality of locally-produced Artemia sp., by evaluation of its essential amino acid and fatty acid profiles after treatments. Our results indicated that Artemia sp. cysts with good quality were produced after 8 hours and Artemia sp. reaches a hatching rate of 1,320,000 cysts (95%) after 27 hours. We also found an indication that the best feed concentration was a mix of 60% Chaetoceros calcitrans with 40% Skeletonema costatum. Fatty acid profile analyses showed that the highest SAFA (12.86%) and PUFA (29.91%) were gained after feeding with Chaetoceros calcitrans, whereas the highest HUFA (4.93%) was gained after feeding with Skeletonemacostatum. Essential amino acid profile analyses revealed the highest content of amino acid (18912.62 ppm) was after feeding with a combination of Chaetoceros calcitrans and Skeletonema costatum.  Finally, the proper water quality during research was at 25-30 oC of temperature, 30-31 ppt of salinity, pH 7.8-8.9, and DO was at 3.0-4.4 mg/L.
Growth and Survival Rate of Tilapia (Oreochromis niloticus) Larvae Fed by Daphnia magna Cultured With Organic Fertilizer Resulted From Probiotic Bacteria Fermentation Vivi Endar Herawati; Johannes Hutabarat; . Pinandoyo; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 22 No. 4 (2015): October 2015
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (580.308 KB) | DOI: 10.4308/hjb.22.4.169

Abstract

Daphnia magna is a potential feed for fish. The aim of this research was to find the best treatment and effect of D. magna culture addition from fermented organic fertilizer, to growth and survival rate of Oreochromis niloticus larvae. There were five treatments, each with three repetitions used in the study. All treatments used chicken dung, and different combinations of rice bran, coconut oilcake waste and tilapia larvae. Feeding on tilapia was given by ad libitum method for five times a day until 14 days. Water quality during the research was maintained at temperature 28–29°C, DO 0.3 ppm and pH 8.1–8.2. Observed variables include relative growth rate, survival rate, food consumption rate and water quality. Our results showed that D. magna cultured by fermented organic fertilizer for tilapia larvae (O. niloticus) had high significant effect (p < 0.01) on the relative growth rate and survival rate. Treatment of D. magna cultured by 1.2 g/L chicken manure, 0.9 g/L rice bran and 0.3 g/L coconut oilcake showed the highest value on the relative growth rate (10.86%); survival rate (98.46%) and food consumption at first week (106.43%) and second week (152.76%).
Maggot Meal (Hermetia illucens) Substitution on Fish Meal as Source of Animal Protein to Growth, Feed Utilization Efficiency, and Survival Rate of Milkfish (Chanos chanos) Vivi Endar Herawati; Pinandoyo Pinandoyo; Seto Windarto; Putut Hariyadi; Johannes Hutabarat; YS Darmanto; Nurmanita Rismaningsih; Slamet Budi Prayitno; Ocky Karna Radjasa
HAYATI Journal of Biosciences Vol. 27 No. 2 (2020): April 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (278.862 KB) | DOI: 10.4308/hjb.27.2.154

Abstract

High protein content in maggot is one of the advantages for increasing fish growth through artificial feed. This study aimed to find the best optimization and feed formulation for fish meal substitution with maggot meal on growth, feed utilization efficiency, and survival rate of milkfish (C. chanos). Milkfish (C. chanos) with an average weight of 0.62±0.01 gram/fish was used as test fish. Feeding is carried out at 07.00 a.m., 12.00 p.m., and 17:00 p.m. through fixed feeding rate method. The tested fish was kept for 42 days with a stocking density of 1 fish/l. The experimental design used was a completely randomized design with five treatments and three replications. The treatments which had been done were fish meal substitution with maggot meal as follows: A (0%), B (25%), C (50%), D (75%), and E (100%). The research parameters included total feed consumption (TFC), feed utilization efficiency (FUE), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and water quality. The results showed that the fish meal substitution with maggot meal had a significant effect (p<0.05) on FUE, PER, RGR and had no significant effect (p>0.05) on TFC and SR. The best treatment of each treatment is in treatment C with a composition of 50% maggot meal substitution on fish meal which resulted in a TFC value of 40.17±4.58, FUE of 27.51±0.77, PER of 0.83±0.03%, and RGR of 2.34±0.10.
PEMANFAATAN CANGKANG KERANG SIMPING (Amusium pleuronectes) SEBAGAI SUMBER KALSIUM PADA PRODUK EKSTRUDAT Tri Winarni Agustini; Susanna Endah Ratnawati; Bambang Argo Wibowo; Johannes Hutabarat
Jurnal Pengolahan Hasil Perikanan Indonesia Vol 14 No 2 (2011): Jurnal Pengolahan Hasil Perikanan Indonesia
Publisher : Department of Aquatic Product Technology IPB University in collaboration with Masyarakat Pengolahan Hasil Perikanan Indonesia (MPHPI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (456.274 KB) | DOI: 10.17844/jphpi.v14i2.5322

Abstract

Asian moon scallop shell can be used as alternative calcium source, and its processing into calcium flour can be used to decrease waste of fresh scallop processing. Asian moon scallop is one of potential commodity alongside east coastal of Central Java. The application of calcium flour in food need to be done as calcium suplement for middle class society. The absorbption of calcium in the body is depending of some factors, one of them is calcium and phosphorus ratio in the food. The aims of study was to examine the effect of addition calcium flour, millet flour and corn flour in extrudate with consideration on ratio of calcium and phosphorus. The parameter analyzed were proximate test, calcium and phosphorus test and physical test (hedonic scale and breaking strength). This research used experimental laboratories and descriptive with Completely Randomised Design (CRD). Data were analyzed by Analysis of Varians (ANOVA) and Honestly Significant Difference (HSD). Hedonic value was tested by Kruskall Wallis analysis. Result show that extrudate snacks gave calcium and phosphorus ratio approaching 3 :  1 (Ca:P). Calcium content in extrudate with modification of calcium flour and corn flour is 582.66 mg/ 100 g and phosphorus content is 180 mg/100 g. Whereas extrudate with modification of calcium flour with millet flour is 950 mg/ 100 and phosphorus content is 280 mg/ 100 g. Breaking strength values in extrudate with modification calcium flour and corn flour are 8.81 kg.F and 5.32 kg.F in modification with calcium flour and millet flour. Values of hedonic test are  6.89 ≤ µ ≤ 7.57 in extrudate modification calcium flour and corn flour and 7.07≤ µ ≤ 7.77 in extrudate modification calcium flour and millet flour. This product has market potential and can be used as calcium source in the society.Key words : calcium flour, Asian moon scallop, formulation and extrudate.
Organic Feed Enrichment Effects toward Growth Performance and Egg Production of Oithona similis Diana Chilmawati; Johannes Hutabarat; Sutrisno Anggoro; Suminto Suminto
Journal Omni-Akuatika Vol 16, No 3 (2020): Omni-Akuatika Special Issue 3rd Kripik SCiFiMaS
Publisher : Fisheries and Marine Science Faculty - Jenderal Soedirman University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.oa.2020.16.3.852

Abstract

The optimum condition of culture media and feed diets which fit the necessities of Oithona similis life (ecophysiological) is very important for it to grow and develop in its maximum. The enrichment of O. similis feed can be done by adding fermented organic feed. Providing organic feed with optimum protein content is necessary to support the growth process and reproduction of O. similis. Osmoregulation process of water organism which is influenced by media salinity is related to bioenergetics process as the effort to use feed energy for the growth. This research aimed to examine the effect of different organic feed protein content and to determine the optimum point which may result in the best growth and egg production of O. similis in the optimum condition of culture media. The study used an experimental method with a completely random design consisting of 4 treatments and 4 repetitions each, i.e. O. similis culture with A. 25%; B. 30%; C. 35%; and D. 40% organic feed protein content. The results showed that the difference of protein content in organic feed affects the growth and production of O. similis eggs. The optimum point of protein content in the fermented organic feed is on protein content B, i.e. 30%, which resulted in the best growth performance (70.44 ± 0.43 ind.mL-1 total density, 0.213 ± 0.001.day-1 population growth rate, and 27.38 ± 0.48 eggs.ind-1 egg production. (ttujuan dan pembahasan harus past tense), Tujuan di abstrak minimal harus menggambarkan atau sama denga tujuan di pendahuluan)Keywords: enrichment, Oithona similis, organic feed, protein, reproduction
DIET DEVELOPMENT FOR MUD CRAB (Scylla serrata) AQUACULTURE USING LOCAL MATERIAL AVAILABLE IN CENTRAL JAVA Johannes Hutabarat
JOURNAL OF COASTAL DEVELOPMENT Vol 2, No 2 (1999): Volume 2, Number 2, Year 1999
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6255.946 KB)

Abstract

Phase I study (1996/1997) indicated that the local protein materials at relatively low cost were found abundance in Central Java throught the year. Their nutritional levels i.e. protein content, amino acids profile and availability are suitable to be used for mud-crab feed development. It is therefore the selected protein materials such as : soybean, mysidaceae, trash fish and saga, are then subjected to the formulation of experimental diet for mud crab on-growing culture (Phase II study 1997/1998) The aims of study are to determine the nutritional performance of experimental diets (novel diets) for mud-crab scylla growth, food utilization and protein efficiency during the feeding trial. The study has been conducted in a 12 bamboo-cages of 1.0 x 0.5 x 0.70 m immersed in BW ponds at CZEL “Prof. Gatot Rahardjo J.S – UNDIP”, Jepara and the stocking density of wild mud-crab (50-60 gram) was 1 pieces /box (0.2 x 0.2 m). Experimental diet (pellet formed) consists of four treatments i.e. diet A (25% dietary protein level), diet B (30%),          diet C (35%) and diet D (40%). The growth and nutritional parameters observed as response to the experimental diets tested are : Individual growth and growth increment, Specific Growth Rate (SGR), FCR, NOU amd PER. The completely Randomize Design was adopted in this study and then followed by Duncans’s New Multiple Range Test in order to determine the difference among the treatments. The results derived from this study indicated that diet C (dietary protein level of 30%) appeared to be the best diet wheather for the growth (W and SGR0 or nutritional performance (FCR, PER, and NPU) and followed by diet B, D and A. Meanwhile, during the course of feeding trial water quality of experimental media remain in good condition to support ud-crab growth.
THE EFFECT OF DIFFERENT C:N AND C:P RATIO OF MEDIA ON THE CONTENT OF POLYHYDROXYBUTYRATE IN BIOFLOC INOCULATED WITH BACTERIUM Bacillus cereus Supono .; Johannes Hutabarat; Slamet Budi Prayitno; YS Darmanto
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 2 (2013): VOLUME 16, NUMBER 2, YEAR 2013
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Biofloc technology  has  added values in aquaculture management, both in water quality  management and feeding  management.  As an optional feed, biofloc is capable to enhance growth due to high protein content.  Bacteria, main component biofloc, can produce polyhydroxybutyrate (PHB) as reserve of energy and growth accelerator for fish. The aim of the research were to study the effect of the different C:N and C:P ratio of media on the content of polyhydroxybutyrate in biofloc and to determine optimum media to produce high polyhydroxybutyrate content in biofloc. The experiment was arranged in factorial  with completely randomized design  in  three replications.  Treatments were C:N ratio of 15, 20, 25 and C:P ratio of 75, 100, and 125. The result showed that C:N ratio and C:P ratio of media and their interaction  affect the content of polyhydroxybutyrate in biofloc.  C:N ratio of 20 and C:P ratio of 125 resulted in most polyhydroxybutyrate (29.25±7.376 mg g-1 biofloc dry weight). Ratio of  C:N  of media gave linier and quadratic responses and C:P ratio of media gave linier one. Optimum polyhydroxybutyrate production was obtained at C:N ratio of 20.9 and C:P ratio of  125 resulting in 29.66 mg  g-1 biofloc dry weight (2,97%)
EFFECT OF USING GUILLARD AND WALNE TECHNICAL CULTURE MEDIA ON GROWTH AND FATTY ACID PROFILES OF MICROALGAE Skeletonema sp. IN MASS CULTURE Vivi Endar; . Sarjito; Johannes Hutabarat; Budi Prayitno
JOURNAL OF COASTAL DEVELOPMENT Vol 16, No 1 (2012): Volume 16, Number 1, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (292.351 KB)

Abstract

Live food, especially microalgae Skelotenoma sp. is a key success factor in shrimp aquaculture. To that end, the provision of Skeletonema sp. mass with a high nutrient content is needed. Nutritional quality of microalgae depends on the culture media used. The purpose of this study was to investigate effect of the use of different technical culture medium (Walne and Guillard) on the growth, protein content and fatty acid profile in microalgae culture Skelotenoma sp. Skeletonema sp. obtained from the Laboratory of Natural Feed BBPBAP Jepara. Culture method used was a mass with two different media (modified Walne and technical Guillard), with 12 replications. Data analysis were analyzed by using T test, while the protein content analysis was performed by Kjedahl method.The fatty acids were determined by using in situ transesterification. The results showed that the growth of Skeletonema sp. was markedly different between media Walne and technical Guillard. Guillard medium revealed lag phase after 44 hours (observation to 6) with a cell density of 48.00 x 104 cells/ml, then entered the exponential phase at 48. (Observation to 7) with a cell density of 70.25 x 104 cells / ml, while the stationary phase occurred in after hours to 52 (observation to 8) with a cell density of 86.75 x 104 cells / ml and death phase began at the 56 ( observations to 9) with a cell density of 54.58 x 104 cells / ml. Growth of Skeletonema sp. cultured with culture medium technical Walne showed a similar pattern in the lag phase to 44 hours of observation (observation to 6 with the cell density is 117.17 x 104sel/ml, exponential phase and stationary phase were detected from hour to 48 (observation to 7) with a cell density is 160.83 x 104 cells / ml. Later phases of death from an hour to 52 (observation to 8) with a cell density of 122.25 x 104 cells / ml. then long culvation or Skeletonema sp stationary phase in Guillard media over a period of 4 hours than Walne medium. Total fatty acids of Skeletonema sp. cultured in Guillard medium resulted in higher yields.
Co-Authors - Masrurotun . Sarjito Abidin Nur II Andi Sagita Anindya Wirasatriya Arumning T. Fauziah Ayu Istiana Fiat Ayudya Wisma Hapsari Aziz Nur Bambang Bambang Argo Wibowo Bella Manik Hapsari Bob Suroso Bosma, Roel Bosma, Roel H. Budi Prayitno Caesa, Genio Cici Ulviyadipura Denny Nugroho Sugianto Diana Chilmawati Diana Chilmawati Diana Rachmawati Diana Rachmawati Dicky Harwanto Eko Nurcahya Dewi Eko Nurcahya Dewi Eko Nurcahya Dewi Eko Nurcahyo Dewi Elsyaday Widhi Cahyono, Elsyaday Widhi Endang Arini Endra Catur Pamungkas Ertris Bergas Taqwdasbriliani Ervia Yudiati Fadityas Desi Aniputri Falstiyan Syahendra, Falstiyan Fatchurochman, Vava Fhibia Jati Fitria Aditama Fritta Wijayanti Hadi Pranggono Haeruddin Haeruddin Hariyadi Hariyadi Hariyadi, Putut Herawati Vivi Endar Hernowo, Ilham Agung Ika Nurul Asriyanti Intan Suriyanti Istiyanto Samidjan Istiyanto Samidjan Ita Widowati Jelita Rahma Hidayati Kunarso Kunarso Lela Nurfitriani Lestari Lakhsmi Widowati Manullang, Corry Yanti Manullang, Corry Yanti Marlia Ulfa Puspitasari Mostafa Imhmed Ighwerb Muhammad Faiq Marwa Noercholis Muhammad Latif Usman Niken Dwi Prasetyarini Norma Afiati Nurmanita Rismaningsih Ocky Karna Radjasa Ocky Karnaradjasa Pane, Pranada Parichat Wetchayount Pinandoyo Pinandoyo Pinandoyo Pinandoyo Pramana, I Nengah Gunaya Pranada Pane Pratama, Arsy Latif Putut Har Riyadi Ratna Widiastuti Ratri, Kartika Sulistyaning Restiana Wisnu Ariyati Retno Hartati Rismaningsih, Nurmanita Ristiawan Agung Nugroho Ristiawan Agung Nugroho Rudhi Pribadi Samsul Rizal Sarjito - Schrama, Johan Seto Windarto Sipayung, Andy Situmeang, Ameria slamet budi prayitno Slamet Budi Prayitno Solly Aryza Sri Rejeki Sri Rejeki Sriwati Sriwati Steven Subandiyono Subandiyono Subekti, Mulat Suhendra Suminto Suminto Suminto Suminto Suminto Suminto Supono . Suradi Wijaya Saputra Suriyanti, Intan Susana E. Ratnawati Susanna Endah Ratnawati Sutrisno Anggoro Tita Elfitasari Titik Susilowati Tony Suhartono Tony Suhartono Tri W. Agustini Tri Winarni Agustini Tri Winarni Agustini Trienes, Yoni Trisnani Dwi Hapsari Tristiana Yunarti Vivi Endar VIVI ENDAR HERAWATI Vivi Endar Herawati Widianingsih Widianingsih Widodo S. Pranowo Widyawati Nurul Fajri Windarto, Seto YS Darmanto