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Lemongrass Powder in Bio-Bs Effervescent Formulation of Lombok Island Local Isolate on Viability and Amount of Bacillus Sphaericus Toxin Crystal Protein for Control of Anopheles sp. Larvae Jiwintarum, Yunan; Fikri, Zainal; Diarti, Maruni Wiwin; Sulaimah, Rabi'unnisa
Jurnal Analis Medika Biosains (JAMBS) Vol 10, No 1 (2023): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v10i1.266

Abstract

Bio Formula – BS effervescent (Bio- Bacillus sphaericus) is an Effervescent powder-shaped formula that is easy to use by the public. Lombok's local effervescent bio-BS isolated formula has a weakness in terms of smell. The results from the literature search that fragrant lemongrass has a distinctive smell and can kill Aedes aeygpti, but scientific data has not been obtained about the ability of fragrant lemongrass to kill Anopheles Sp in the form of larvae and mosquitoes. The study aimed to find out the effect of the combination formulation of Bio-BS Effervescent local isolate Lombok Island with the addition of lemongrass powder for viability and the amount of toxin crystal protein Bacillus sphaericus. This research method is exploratory and experimental in the laboratory with the design of Post Test Only Control Group Design. The study used six treatment formulations. Samples of Anopheles sp larvae research in Batu Layar lagoon, West Lombok regency and pelur lagoon in Peringgesela, East Lombok regency and from the results of colonization of larvae. The independent Variable is a combination formulation of Bio-BS Effervescent isolated locally from Lombok Island with the addition of fragrant lemongrass powder. The dependent variable is the mortality of Larvae Anophels Sp, Viability of B. Sphaericus, and Amount of Toxin Protein Production of B. Sphaericus. The larvae death rate, the concentration of cells/endospores, and the number of repeats in each container are then tabulated and analyzed using Probit Analysis with the help of MINITAB 16 software. B. sphaericus viability data and the amount of endospore toxin protein crystal production were descriptively analyzed.
Jenis dan Jumlah Sedimen Urine Organik dan Anorganik pada Penderita Demam Typhoid Sebelum dan setelah Pemberian Antibiotik Diarti, Maruni Wiwin; Fikri, Zainal; Sintia Dewi, Ni Kadek; Jiwintarum, Yunan
Jurnal Analis Medika Biosains (JAMBS) Vol 8, No 1 (2021): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v8i1.222

Abstract

Salmonella typhi merupakan kuman pathogen penyebab demam typhoid yaitu suatu penyakit infeksi sistemik dengan gejala demam, gangguan saluran cerna, dan mual muntah. Demam dengan suhu yang tinggi, akan terjadi komplikasi seperti perubahan pada urin yang menjadi lebih gelap karena pecahnya sejumlah sel darah merah, dan gagal ginjal. Diagnosis tersebut perlu membutuhkan pemeriksaan, seperti pemeriksaan sedimen urine. Tujuan dari penelitian ini adalah untuk mengetahui perbedaan jenis dan jumlah sedimen urine pada penderita demam typhoid sebelum dan setelahpemberianAntibiotik. Penelitian ini menggunakan rancangan penelitian observasional deskriptif dengan pendekatan cross sectional. Populasi pasien yang positif demam typhoid di Puskesmas Gerung Kabupaten Lombok Barat dan sampel sebanyak 23 orang. Analisis data menggunakan deskriptif.  Hasil penelitian menunjukkan jumlah sedimen urine sebelum pemberian Antibiotik adalah leukosit dan eritrosit abnormal (13,0%), epitel abnormal (47,8%), Kristal klasium abnormal (4,3%). Jumlah sedimen urine setelah pemberian Antibiotik adalah leukosit normal (100%), tidak ditemukan leukosit abnormal, eritrosit normal (56,5%) dan tidak ditemukannya eritrosit abnormal, sel epitel normal (60,8%), dan tidak ditemukannya sel epitel abnormal.
Resistance Analysis Of Several Antibiotics in Samples of Clinical Isolates With Salmonellosis Wahyuni, Shohifatul; Pauzi, Iswari; Jiwintarum, Yunan; Fihiruddin, Fihiruddin
Jurnal Analis Medika Biosains (JAMBS) Vol 11, No 1 (2024): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v11i1.357

Abstract

Typhus is a systemic infection caused by Salmonella typhi bacteria, which is usually transmitted through contaminated food or drink, usually treated with antibiotics. Inappropriate use of antibiotics causes various problems, one of which causes resistance. This resistance problem has become a global problem, including in Indonesia where there is an increase in bacterial resistance from 2013 which is 40%, 2016 as much as 60% and in 2019 reached 60.4%, due to irrational use of antibiotics so that bacteria become resistant to drugs. This study aims to determine the description of resistance to several antibiotics in clinical isolates of patients with Salmonellosis This study is a pre-experimental study, with a cross sectional approach. The samples used were pure isolates of Salmonella typhi bacteria obtained from blood samples of Salmonellosis patients, then tested for resistance with 5 different types of antibiotics (Ciprofloxacin, Amoxicillin, Levofloxacin, Cefotaxime and Sulfamethoxazolle-trimethopim) with the disk method (Kearby Beaure). The number of experimental units is 25 units, with the number of replications is 5 replications. The results showed that the five types of antibiotics were sensitive to Salmonella typhi bacteria with the highest antibiotic sensitivity being Ciprofloxacin while the antibiotic that had the lowest sensitivity was Amoxicillin. From the results, it can be concluded that the resistance test of the five antibiotics (Ciprofloxacin, Amoxicillin, Levofloxacin, Cefotaxime and Sulfamethoxazolle-trimethopim) is sensitive. 
Hematocrite Values With High Measurement Of Eritrosit After Centrifugation On Serum Making Jiwintarum, Yunan; Srigede, Lalu; Asyhaer, Rifki Khalidi
Jurnal Analis Medika Biosains (JAMBS) Vol 7, No 2 (2020): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v7i2.193

Abstract

Tujuan Penelitian ini untuk mengetahui apakah nilai hematokrit darah dapat diukur dengan pengukuran tinggi endapan eritrosit setelah disentrifugasi pada pembuatan serum.  Jenis penelitian ini adalah Quasi Eksperiment dengan rancangan Posttest Only Control Group Design. Banyaknya perlakuan ada tiga, yaitu pembuatan serum dengan sentrifugasi kecepatan 3000 rpm selama 5 menit, 10 menit, dan 15 menit.  Data yang dikumpulkan berupa nilai hematokrit dengan sentrifugasi pada pembuatan serum dibandingkan dengan nilai hematokrit dengan metode mikrohematokrit. Data dianalisis menggunakan uji Kruskal-Wallis. Hasil menunjukkan bahwa rerata nilai hematokrit dengan metode mikrohematokrit adalah 46%, sedangkan rerata nilai hematokrit dengan pengukuran tinggi endapan eritrosit pada pembuatan serum setelah disentrifugasi selama 5 menit, 10 menit, dan 15 menit adalah 58,5%, 57,6%, dan 48,1%. Uji statistik menunjukkan adanya perbedaan yang signifikan hasil pemeriksaan metode mikrohematokrit dan pemeriksaan dengan sentrifugasi 5 menit maupun 10 menit. Sedangkan antara metode mikrohematokrit dan pemeriksaan dengan sentrifugasi 15 menit tidak ada perbedaan yang signifikan. Kesimpulan : Pengukuran tinggi endapan eritrosit setelah disentrifugasi selama 15 menit pada pembuatan serum dapat digunakan untuk pemeriksaan hematokrit.
Hubungan antara Nilai Hemoglobin Glikasi (HbA1c) Dengan Kadar Ureum dan Kreatinin pada Pasien Diabetes Melitus Tipe 2 Di RSUD Praya Suda Adnyana Yoga, I Gede; Jiwintarum, Yunan; Urip, Urip; Pauzi, Iswari
Jurnal Borneo Cendekia Vol 9 No 1 (2025): Jurnal Borneo Cendekia
Publisher : STIKES Borneo Cendekia Medika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54411/jbc.v9i1.621

Abstract

Diabetes melitus (DM) menjadi persoalan kesehatan yang serius di Nusa Tenggara Barat (NTB) dan dapat menimbulkan komplikasi jangka panjang, seperti nefropati diabetik yang menyebabkan gagal ginjal. Kondisi hiperglikemia kronis pada penderita diabetes menyebabkan perubahan pada struktur dan fungsi ginjal melalui mekanisme seperti produk akhir glikasi lanjut (AGEs), stres oksidatif, dan proses inflamasi. Oleh karenanya, pengendalian glikemik yang tepat sangat penting untuk mencegah timbulnya penyakit ginjal kronis (PGK) pada pasien diabetes. Penelitian kuantitatif dengan rancangan cross-sectional ini bertujuan untuk mengevaluasi hubungan antara kadar HbA1c dan kadar ureum serta kreatinin pada pasien dengan DM tipe 2 di RSUD Praya. Sebanyak 92 sampel dipilih menggunakan teknik purposive sampling dari data rekam medis sekunder. Analisis korelasi Spearman digunakan untuk menilai hubungan antar variabel tersebut. Mayoritas sampel adalah pria (52,2%) dan sebagian besar menunjukkan peningkatan kadar glukosa (70,7%). Dari sampel tersebut, 67,4% memiliki kadar HbA1c lebih dari 7%, sementara kebanyakan memiliki kadar ureum normal (60,9%) dan kreatinin normal (81,5%). Hasil analisis korelasi tidak menunjukkan hubungan signifikan antara kadar HbA1c dengan ureum (p=0,127; koefisien korelasi=0,160) maupun dengan kreatinin (p=0,803; koefisien korelasi=0,026). Tidak terdapat hubungan yang bermakna antara kadar HbA1c dan kadar ureum maupun kreatinin. Hal ini mengindikasikan bahwa nilai HbA1c tidak mencerminkan secara signifikan penurunan fungsi ginjal. Oleh sebab itu, pemantauan glikemik pada pasien diabetes perlu dilengkapi dengan pemeriksaan kadar ureum dan kreatinin secara rutin untuk memperoleh gambaran menyeluruh mengenai fungsi ginjal.
Differences in Blood Glucose Levels Before and After the Consumption of Sweet Tea in People in Marong Village Aprilla, Annisa Suci; Diarti, Maruni Wiwin; Pauzi, Iswari; Jiwintarum, Yunan
Jurnal Analis Medika Biosains (JAMBS) Vol 12, No 2 (2025): JURNAL ANALIS MEDIKA BIOSAINS (JAMBS)
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jambs.v12i2.454

Abstract

Blood glucose is the body's main energy source and is greatly influenced by the consumption patterns of food and beverages, especially those containing sugar. Uncontrolled increases in blood glucose levels can lead to metabolic disorders such as Diabetes Mellitus. Consumption of sweetened tea containing added sugar can cause a rapid spike in blood glucose levels, especially when consumed regularly and in excessive amounts. The purpose of this study is to find out the difference in blood glucose levels before and after sweet tea consumption in the Marong Village community. This study uses a pre-experimental design with a one-group pretest-posttest approach. A total of 16 respondents were selected using the purposive sampling technique. Blood glucose levels were checked before and two hours after the consumption of sweet tea using the POCT (Point of Care Testing) tool. Data were analyzed using a statistical paired sample t-test. The results of the study showed obtained the average blood glucose level before the consumption of sweet tea was 88.3 mg/dL, and after consumption was 97.3 mg/dL, with an average difference of 8.3 mg/dL. The results of the paired sample t-test showed a significance value of 0.000 (p < 0.05), which means that there is a statistically significant difference. This study concludes that the consumption of sweet tea has a significant influence on increasing blood glucose levels.  
Effect of Aloe vera Gel Formulation and Jatropha multifida L Leaf on the Growth of Staphylococcus aureus Bacteria Dektra, I Made Putra Pramaditya; Pauzi, Iswari; Srigede, Lalu; Jiwintarum, Yunan
Hydrogen: Jurnal Kependidikan Kimia Vol. 13 No. 4 (2025): August 2025
Publisher : Universitas Pendidikan Mandalika

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/hjkk.v13i4.17104

Abstract

Staphylococcus aureus bacteria is one of the agents of health problems that require special attention. Inappropriate use of antibiotics can cause resistance, so a more effective and safe alternative treatment is needed, one of which is by giving aloe vera gel formulations and tintir castor leaves that can help overcome bacterial infections, such as Staphylococcus aureus. Knowing the effect of aloe vera gel formulation (Aloe vera) and tintir castor leaf (Jatropha multifida) on the growth of Staphylococcus aureus bacteria. The study was pre-experimental with a cross sectional design. Gel formulations were made with various comparisons between aloe vera gel and tintir castor leaf, then tested against Staphylococcus aureus bacteria by observing the inhibition zone as an indicator of antibacterial activity. The results of testing the Aloe vera gel formulation and castor leaf filtrate showed that there was no inhibition zone in each of the tested formulation ratios. In the testing using Aloe vera gel formulations and Jatropha multifida leaf extract, no inhibition zones were observed in the 1:1 and 1:3 ratios, but an inhibition zone was present in the 3:1 ratio, measuring 4–6 mm with an average inhibition zone of 5 mm. This result can be considered sensitive but at a low level for inhibiting bacterial growth. There is an effect of Aloe vera gel and jarak tintir leaf formulation on the growth of Staphylococcus aureus bacteria.
Differences in Cholesterol Levels in Coffee Drinkers Without Sugar and Coffee Drinkers With Sugar in The Work Area Salsabila, Nadira; Khusuma, Ari; Jiwintarum, Yunan
THRIVE Health Science Journal Vol. 1 No. 1 (2024): March
Publisher : Balai Publikasi Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56566/thrive.v1i1.93

Abstract

Indonesia is one of the coffee-producing countries as well as coffee bean exporters which ranks fourth in the world. Coffee is one of the most consumed beverages in the world which has become a major need for people without realizing it. Based on the results of previous studies that coffee drinkers using sugar can increase cholesterol levels. Excessive cholesterol in the body will accumulate in the walls of blood vessels and cause a condition called atherosclerosis, namely narrowing or hardening of the arteries. This condition is the forerunner of heart disease and stroke. This study aims to determine differences in cholesterol levels in coffee drinkers without sugar and coffee drinkers using sugar in the working area of Karang Pule. This research is an analytical observation using capillary blood samples of respondents who drink coffee without sugar and drink coffee with sugar. The data obtained in this study were recorded and presented in tabular form and analyzed descriptively. From the results of the study, it was found that the results of examining cholesterol levels in coffee drinkers without sugar were 15 people with an average cholesterol level of 169 mg/dl. Meanwhile, coffee drinkers using sugar were 15 people with an average cholesterol level examination of 242 mg/dl
Gambaran Kadar Trigliserida pada Wanita Menopouse dengan dan tanpa Hipertensi Hapielda, Ririn; Resnhaleksmana, Dr.Ersandhi; Wayan Getas, Drs.I; Jiwintarum, Yunan
Journal of Indonesia Laboratory Students (JILTS) Vol. 3 No. 1 (2024): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v3i1.46

Abstract

Latar belakang : Peningkatan trigliserida terjadi pada fase menopouse. Jika kadar trigliserida meningkat mengakibatkan penumpukan lemak dalam aliran darah yang menimbulkan plaque sehingga aliran darah terhambat yang dapat memicu terjadinya hipertensi. Tujuan : Tujuan penelitian ini adalah untuk mengetahui gambaran kadar trigliserida pada wanita menopouse dengan dan tanpa hipertensi. Dengan data yang disajikan dan dianalisis secara deskriftif. Metode : Jenis peneltian ini purposive sampling. Pengambilan sample dilakukan dengan wawancara sesuai kriteria sebanyak 32 sample dibagi 2 kategori. Kadar trigliserida pada penelitian ini diperoleh dengan metode automatically analyzer dan data diolah secara deskriptif Observasional. Hasil : Dari hasil penelitian didapatkan bahwa Rata -rata kadar trigliserida pada wanita menopouse dengan hipertensi dari hasil penelitian ini adalah 177 mg/dL dengan tekanan darah 151/92 mmHg dan rata – rata kadar trigliserida pada wanita menopouse tanpa hipertensi adalah 148 mg/dL dengan rata-rata tekanan darah 110/79 mmHg. Kesimpulan : Dapat disimpulkan bahwa kadar trigliserida pada wanita menopouse dengan hipertensi lebih tinggi dibandingkan dengan kadar trigliserida pada wanita menopouse tanpa hipertensi.
Pengaruh Lama Penyimpanan Darah terhadap Kualitas Darah Packed Red Cell (PRC) di UTD RSUD Kabupaten LombokUtara Adawiyah, Luluil; Jiwintarum, Yunan; Resnhaleksmana, Ersandhi; Urip
Journal of Indonesia Laboratory Students (JILTS) Vol. 4 No. 1 (2025): Journal of Indonesia Laboratory Students
Publisher : Poltekkes Kemenkes Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32807/jilts.v4i1.192

Abstract

Background: Packed Red Cell (PRC) is the most widely used blood component in transfusion. In general, PRC is used for patients with anemia that is not accompanied by a decrease in blood volume, such as patients with hemolytic, acute leukemia, chronic leukemia, thalassemia, and chronic renal failure. The quality of the PRC component is done by checking the hemoglobin and hematocrit levels in the blood component products produced. In transfused blood storage there are elements of blood storage lesions including: morphological changes, slowing metabolism with decreased adenosine triphosphate (ATP) concentration, acidosis with decreased 2,3 diphosphoglycerate (2,3-DPG) concentration. This will pose a risk to the safety and efficacy of long-stored PRC.. Research Objectives: Knowing the Effect of Blood Storage Duration on the Quality of Packed Red Cell (PRC) Blood in the Blood Transfusion Unit of North Lombok Regency Hospital. Research Methods: This research is a pre-experimen The study population was all PRC blood in the Blood Transfusion Unit of NorthLombok District Hospital. The sample used was PRC blood taken randomly with a total of 24 experimental units. Using primary data and statistically analyzed with Kruskal-Wallis test. The Results: The average hemoglobin level on day 21 storage was 50.3 g/unit, day 28 was 48.8 g/unit, and on day 35 was 47.1 g/unit. The average hematocrit value on day 21 of storage was 65.6%, day 28 was 63.6%, and on day 35 was 61.4%. The results of the PRC blood quality assessment showed that on day 21 storage the blood quality was good, while on day28 and day 35 storage the PRC blood quality was not good. Effect of bloodstorage duration on PRC blood quality (p = 0.000 < α = 0.05). Conclusion: There is an effect of storage duration on the quality of PRC blood.
Co-Authors AA Sudharmawan, AA Achmad Fathoni Adawiyah, Luluil Aji Kresnapati, I Nyoman Bagus Anas Fadli Wijaya Anggun Sari Mianti Aprilla, Annisa Suci Asyhaer, Rifki Khalidi Atmojo, Joko Tri Auliya Rahmawati Awan Dramawan Ayu Anulus Ayu Rosita ’diah, Halimatussa Dektra, I Made Putra Pramaditya Dewi, Lale Budi Kusuma Duarsa , Artha Budi Susila Eliza, Ida Eliza, Ida Erdianti Nursahar Erlin Yustin Tatontos Ershandi Resnhaleksmana Fachrudi Hanafi Fihiruddin Fihiruddin, Fihiruddin Fitria Ernawati Getas, I Wayan Gunarti Gunarti Gunarti, Gunarti Habib Sasiwimbe Hapielda, Ririn Hardiyanti, Uci Haris Widita Herawati, Lale Heny I Dewa Putu Martha Prayuda I Gede Angga Adnyana I Putu Bayu Agus Saputra Ida Ayu Reka Iswari Pauzi Iswidhani, Iswidhani Khairunnisa Khusuma, Ari Kresnapati, I Nyoman Bagus Aji Lalu Srigede Lalu Srigede, Lalu Liana Dwihartati Mardiatuz Zahra Marnia, Marnia Maruni Wiwin Diarti Maruni Wiwin Diarti Nurhidayati Nurhidayati Pancawati Ariami Pauzi, Iswari Putra, I Gede Ari Permana Rahmani, Putri Ziddanur RAI WIADNYA, IDA BAGUS Rauhul Akbar Kurniawan Resnhaleksmana, Dr.Ersandhi Resnhaleksmana, Ersandhi Retno Handayani Retno Handayani Rohmi Rohmi Rohmi, Rohmi saadaty, nila uthari Sahputri, Maulidia Rohmiati Salsabila, Nadira Shohifatul Wahyuni Sintia Dewi, Ni Kadek Siti Zaetun, Siti Soewignjo Soemohardjo Suda Adnyana Yoga, I Gede Sukma, Al Hadawiyah Pertiwi Sukmawati, Sulvani Sulaimah, Rabi'unnisa Sulvani Sukmawati Troef Sumarno Urip Urip Urip Urip Urip, Urip Wahyuni, Shohifatul Wayan Getas, Drs.I Weny Astuti Widianto, Aris Yuliana Wardani Yuri Syaffitri Kinanti Achmad Zaeniati, Baiq Lely Zainal Fikri Zainal Fikri Zainal Fikri, Zainal Zainul Mutaqin ’diah, Halimatussa