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Utilization of Chitosan Elicitation on Biomass, Total Flavonoids, and Antioxidant Activity of Black Betel Callus (Piper betle L. var Nigra): Utilization of Chitosan Elicitation on Black Betel Callus (Piper betle L. var Nigra) Nurindah Wanti, Yulia Tri; Wida Utami, Edy Setiti; Junairiah
Journal of Tropical Life Science Vol. 15 No. 2
Publisher : Journal of Tropical Life Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/3d65dk31

Abstract

Callus culture is a suitable method for the production of secondary metabolites. Elicitors can increase the content of secondary metabolites through the elicitation process. This study aims to determine the effect of chitosan elicitation on biomass, total flavonoids, and antioxidant activity of black betel callus. The research method is callus production by growing leaf explants on Murashige and Skoog (MS) medium with the addition of growth regulators Benzyl Amino Purin (BAP) 0.5 mg/L and 2,4-Dichlorophenoxy Acetic Acid (2,4-D) 2.0 mg/L. Furthermore, the callus was sub-cultured on MS medium with chitosan elicitor for four days. The data obtained was callus biomass. Extraction of callus to measure flavonoid content and antioxidant activity. The results showed that chitosan elicitation at 1.5 mg/L showed the most optimal biomass results, 1.22  ± 0.39 g fresh weight and 0.81 ± 0.17 g dry weight. Chitosan elicitation with 1.5 mg/L treatment showed the highest total flavonoid content of 9.52 ± 0.37 µg QE/g. The methanol extract of black betel callus resulting from chitosan elicitation has antioxidant activity. Callus treated with 2 mg/L chitosan showed the most substantial IC50 value of 53.60 ± 14.45 mg/L. Chitosan elicitation can effectively increase biomass, total flavonoids, and antioxidant activity of black betel leaf callus. Chitosan 1.5 mg/L is the optimum concentration for increasing biomass and total flavonoids, and chitosan 2 mg/L is the concentration that has the most effect on the antioxidant activity of black betel leaf callus.
Co-Authors A.A. Ketut Agung Cahyawan W Agus Supriyanto Alfiah Hayati Almando Geraldi Anjani, Ajeng Distya Anjar Tri Wibowo Annisa Azzahra, Annisa Diah Ayu Wulandari Dwi Kusuma Wahyuni Dwi Winarni Edy Setiti Wida Utami Eva Hariyanti Fatimah fatimah Fatimah Febrionny, Princessa Nandita Firli Rahmah Primula Dewi Hamidah Hamidah . Hapsari, Lukiteswari Dyah Tri Hery Purnobasuki Hery Suwito Iftitah, Daulah Intan Ayu Pratiwi, Intan Ayu Irfansyah, Febri Dwi Lilis Sulistyorini Listijani Suhargo, Listijani Manikya Pramudya Mochammad Affandi Muhammad Hilman Fu'adil Amin Nabilah Istighfari Zuraidassanaaz Nabilah Istighfari Zuraidassanaaz Nabilah Istighfari Zuraidassanaaz Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni'matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Niken Agustin Nimatuzahroh, Nimatuzahroh Ni’matuzahroh Ni’matuzahroh Ni’matuzahroh, Ni’matuzahroh Ni’matuzzahroh Ni’matuzahroh Nurindah Wanti, Yulia Tri Nurul Sofi Amalia Oktavia, Gebby Agnessya Esa Purnomo Purnomo Purnomo Purnomo Putri Akustia Putri, Salsabilla Wiyana Rachmah, Artifa Sa'diyah, Muhimmatus Salamun Salamun Shabrina, Afrah Sofiana, Dewi Amelia Sri Puji Astuti Wahyuningsih, Sri Puji Astuti Surahmaida, Surahmaida Syarifah Salsabila Tamalia, Aqilla Kalyca Tri Nurhariyati Tri Puji Lestari Sudarwati Ulinnuha, Risanda Umarudin, Umarudin Wanti, Yulia Tri Nurindah Wida Utami, Edy Setiti Yosephine Sri Wulan Manuhara Zuraidassanaaz, Nabilah Istighfari