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All Journal HAYATI Journal of Biosciences Jurnal Ilmu dan Teknologi Kelautan Tropis Jurnal Rekayasa Proses JOURNAL OF COASTAL DEVELOPMENT ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Ilmu Lingkungan Journal of Marine Research Microbiology Indonesia OSEATEK DIPOIPTEKS: Jurnal Ilmiah Mahasiswa Undip Jurnal Matematika & Sains Prosiding Indonesian Journal of Pharmaceutical Science and Technology Proceeding Seminar LPPM UMP Tahun 2014 Indonesian Journal of Biotechnology Jurnal Kelautan Tropis Squalen Bulletin of Marine and Fisheries Postharvest and Biotechnology Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Marine Research in Indonesia Biosaintifika: Journal of Biology & Biology Education Jurnal Rekayasa Proses Indonesian Journal of Energy Makara Journal of Science Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) Prosiding University Research Colloquium Prosiding Konferensi Nasional Penelitian Matematika dan Pembelajarannya
Ocky Karna Radjasa
Badan Riset Dan Inovasi Nasional
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Journal : Journal of Marine Research

 1 
POTENSI PIGMEN KAROTENOID BAKTERIUM ENDOFIT LAMUN Thalassia hemprichii SEBAGAI SUMBER SENYAWA ALAMI PENANGKAL RADIKAL BEBAS DPPH (1,1-Difenil-2-Pikrilhidrazil) Permata, Rr. Citra; Riniatsih, Ita; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (178.42 KB) | DOI: 10.14710/jmr.v3i3.6001

Abstract

Karotenoid merupakan salah satu jenis pigmen yang penting bagi kesehatan manusia yang berfungsi sebagai antioksidan. Karotenoid merupakan pigmen kuning, orange sampai merah yang biasanya ditemukan dalam sayuran, buah-buahan serta bakteri dan fungi. Penelitian ini bertujuan mengetahui aktivitas antioksidan dari pigmen karotenoid bakteri endofit lamun Thalassia hemprichii serta mengidentifikasi bakteri tersebut secara molekuler. Analisis pigmen dilakukan dengan menggunakan spektrofotometer UV 1601, uji antioksidan dengan metode DPPH dan identifikasi bakteri dilakukan dengan PCR 16S rDNA. Penelitian ini dilaksanakan pada bulan Oktober 2013 – April 2014. Sampel lamun didapatkan dari perairan Teluk Awur Jepara. Hasil penelitian menunjukkan bahwa bakterium Th1 mempunyai pigmen kuning dengan tiga puncak panjang gelombang yaitu 460 nm, 439.5 nm, 417 nm sesuai dengan serapan panjang gelombang pigmen karotenoid yaitu 300-600 nm. Identifikasi bakteri secara molekuler 16S rDNA menunjukkan bahwa isolat Th1 mempunyai homologi 97% dengan Erythrobacter vulgaris. Aktivitas penghambatan pigmen bakteri Th1 yaitu 18,97%.. Berdasarkan hasil penelitian dapat disimpulkan bahwa pigmen karotenoid bakterium Th1 mempunyai potensi sebagai senyawa alami penangkal radikal bebas DPPH.
IDENTIFIKASI PIGMEN KAROTENOID PADA BAKTERI SIMBION RUMPUT LAUT Kappahycus alvarezii Sahara, Fera Nur Idawati; Radjasa, Ocky Karna; Supriyantini, Endang
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (433.865 KB) | DOI: 10.14710/jmr.v2i3.3133

Abstract

Carotenoid is one of the most important pigments that have important roles for human health. Carotenoids are believed to improve the better immune responses, anticancer, antioxidant, provitamin A and are also used in the treatment of disease that are sensitive to light. Carotenoids are yellow pigments, orange to red pigments and usually found in vegetables and fruits, and are also found in animals, humans, fungi and bacteria. The aims of study were to analyze carotenoid pigments from bacterial symbionts from seaweed Kappaphycus alvarezii and to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by a UV-VIS spectrophotometer and High Performance Liquid Chromatography (HPLC). Bacterial identification was performed based on biochemical tests. These results showed that from 12 bacterial symbionts isolated, there were 2 isolates that positively synthesize carotenoids pigments, ECJ K and ECJ OR bacteria. ECJ K bacterium contained of Violaxanthin and α-carotene pigments. While ECJ OR bacterium contained of Diadinoxanthin, Neoxanthin, γ-carotene, β-carotene and α-carotene pigments. Bacterial identification showed that ECJ K bacterium was Brevibacterium maris and ECJ OR bacterium was Micrococcus varians. This result showed that bacterial symbionts of seaweed Kappaphycus alvarezii contained various pigments including in carotenoid pigments.
Uji Fitokimia dan Aktivitas Antijamur Ekstrak Teripang Keling (Holoturia atra) Dari Pantai Bandengan Jepara Terhadap Jamur Candida albicans Septiadi, Tedi; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (158.857 KB) | DOI: 10.14710/jmr.v2i2.2355

Abstract

Holothuria atra is one of the marine lifes that lives at the bottom of the substrate, and able to adapt to its environment. Several previous studies showed that extracts of H. atra from different waters have activity as an antifungal. Fungus Candida albicans is one of human pathogens that attack on the mucosa of the mouth, skin and vagina. The purpose of this study were to identify the compounds contained in the extracts of H.atra and examine the effect of extracts concentration against C. albicans. The process of extraction was done by maceration with solvent n-hexane, ethyl acetate and methanol. Testing of secondary metabolites was carried out using phytochemical screening methods while testing antifungal activity was using agar diffusion test. The results showed that the extracts of H.atra contained saponins, alkaloids, steroids and triterpenoids. Antifungal activity assays showed that the n-hexane extract did not show any inhibition zone, while the ethyl acetate and methanol extracts showed inhibition zone at a concentration of 1 mg / disk with a large zone of inhibition of 8.27 ± 0.06 and 8.07 ± 0 , 12 mm, respectively based on these results it can be concluded that the extract of ethyl acetate solvent H.atra has strong potential as antifungal.
IDENTIFIKASI PIGMEN KAROTENOID PADA BAKTERI SIMBION RUMPUT LAUT Caulerpa cupressoides (Vahl) C. Agardh Arlita, Nurul Ria; Radjasa, Ocky Karna; Santoso, Adi
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (197.73 KB) | DOI: 10.14710/jmr.v2i3.3134

Abstract

One of natural pigments besides Chlorophyll is carotenoid that has two classes, namely Xanthophyll and Carotene that have important roles for human helath like provitamin A, antioxidant, anticancer, and to improve the better immune responses. Carotenoids are yellow pigments, orange to red pigments that can be found in human, animals, high level plants, fruits, algae, fungi and bacteria. Bacterial symbionts produce pigments that are similar with their host. Pure cultures from marine microbial community can be isolated and cultivated in big scale in relatively short time. The purpose of this study was to as well as isolate and to identify pigments carotenoid from bacterial symbionts from seaweed Caulerpa cupressoides to identify bacterial symbionts that produce carotenoid pigments. Pigment analysis was performed by UVVis spectrophotometer and High Performance Liquid Chromatography (HPLC). Bacterial identification was performed based on biochemical tests. These results showed that from 13 bacterial symbionts isolated from Teluk Awur Waters, there was 2 isolates that positively synthesize carotenoids pigments, CJ.K and CJ.OR bacteria respectively, CJ.K bacterium contained of Violaxanthin and Neoxanthin pigments. While CJ.OR bacterium contained of Xanthophyll, Diadinoxanthin, γ-carotene, and β-carotene pigments. Bacterial identification showed that CJ.K bacterium was Brevibacterium maris and CJ.OR bacterium was Paracoccus alcaliphilus. This result showed that found some carotenoid pigments from bacterial symbiont of seaweed Caulerpa cupressoides.
POTENSI RUMPUT LAUT Sargassum duplicatum SEBAGAI SUMBER SENYAWA ANTIFOULING Santi, Ika Wulan; Radjasa, Ocky Karna; Widowati, Ita
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.282 KB) | DOI: 10.14710/jmr.v3i3.5999

Abstract

Biofouling di laut menyebabkan kerusakan pada lambung kapal dan merusak infrastruktur kelautan lainnya. Rumput lautgenus Sargassum banyak dilaporkan sebagai alternatif sumber senyawa antifouling alami yang ramah lingkungan. Penelitian ini menggunakan S. duplicatum dari Perairan Teluk Awur Jepara yang diekstrak dengan berbagai pelarut (n-heksana, etil asetat dan metanol) untuk mengetahui potensi ekstrak sebagai antifouling dan golongan senyawanya serta untuk mengidentifikasi jenis bakteri biofilm yang sensitif terhadap ekstrak. Bakteri biofilm diisolasi dari panel kayu dan fiberglass yang dibenamkan dilaut selama 2 minggu. Uji aktivitas antimicrofouling menggunakan metoda disc diffusion, uji fitokimia menggunakan metoda Harborne (1987) dan uji biokimia menggunakan metoda Cowan and Steels (1974) dan Bergey’s (2005). Aktivitas antimicrofouling paling baik ditunjukan oleh ekstrak etil asetat yang dapat menghambat 22 dari 34 bakteri biofilm dengan kisaran zona hambat 0,65 – 3,73 mm. Sedangkan, bakteri yang paling sensitif terhadap ekstrak S. duplicatum adalah bakteri K.10.3.1.4 dan F.10.3.1.2. S. duplicatum mengandung senyawa golongan alkaloid, saponin, quinon, fenolik, steroid, dan flavonoid. Berdasarkan identifikasi bakteri secara biokimia, bakteri K.10.4.1.5 adalah genus Achromobacter, sedangkan kode isolat F.10.3.1.2 adalah genus Flavobacterium cytophaga. Kehadiran satu ataulebih senyawa fitokimia dalam ekstrak diduga bertanggung jawab atas aktivitas antifouling yang terjadi.
SKRINING ANTIBAKTERI EKSTRAK RUMPUT LAUT Sargassum plagyophyllum DARI PERAIRAN BANDENGAN JEPARA TERHADAP BAKTERI PATOGEN Enterobacter, Pseudomonas aeruginosa DAN Staphylococus aureus Yunianto, Hendi Perdian; Widowati, Ita; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (239.443 KB) | DOI: 10.14710/jmr.v3i3.5987

Abstract

Sifat resistensi dan infeksi patogenitas bakteri terhadap manusia saat ini meningkat, maka perlu adanya obat alternatif baru. Senyawa bioaktif yang terkandung dalam rumput laut merupakan salah satu sumber antibakteri baru yang diperoleh dari alam. Tujuan penelitian ini adalah mengetahui aktivitas antibakteri ekstrak Sargassum plagyophyllum terhadap bakteri patogen Enterobacter, P. aeruginosa dan S. aureus serta mengetahui golongan senyawa dan toksisitas senyawa bioaktif ekstrak S. plagyophyllum. Penelitian ini dilakukan pada bulan September 2012 – Mei 2013. Pengambilan sampel dilakukan secara purposive sampling method. Metode yang digunakan dalam penelitian ini adalah metode eksperimental laboratoris. Ekstraksi dilakukan secara bertingkat dengan menggunakan tiga pelarut yang berbeda kepolarannya. Uji aktivitas antibakteri dilakukan menggunakan metode difusi agar (Kirby-bauer) dengan 3 kali pengulangan. Analisis fitokimia dilakukan secara deskriptif kualitatif berdasarkan perubahan warna serta karakteristik fisika kimia suatu golongan. Uji toksisitas dilakukan menggunakan larva Artemia salina. Nilai toksisitas akut (LC50) ditentukan dengan menggunakan persamaan regresi linier. Hasil penelitian menunjukkan bahwa ekstrak Sargassum plagyophyllum pelarut etil asetat memiliki aktivitas antibakteri terbaik dengan zona hambat 4,87 ± 0,49 (bakteri Enterobacter); 6,06 ± 0,58 (bakteri S. aureus) dan 6,30 ± 0,62 (bakteri P. aeruginosa) serta memiliki aktivitas bakteriosidal. Hasil analisis fitokimia menunjukkan bahwa secara umum ekstrak Sargassum plagyophyllum mengandung senyawa alkaloid (pelarut heksana), steroid (ketiga pelarut), saponin (pelarut metanol). Hasil uji toksisitas menunjukkan ekstrak S. plagyophyllum pelarut etil asetat memiliki toksisitas yang toksik dengan nilai LC50-24 jam sebesar 291 ppm (toksik kategori kronik).
Uji Fitokimia Dan Toksisitas Ekstrak Kasar Gastropoda (Telescopium telescopium) Terhadap Larva Artemia salina Putri, Mukti K. Diana; Pringgenies, Delianis; Radjasa, Ocky Karna
977-2407769
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.912 KB) | DOI: 10.14710/jmr.v1i2.2020

Abstract

Gastropod is one of the marine organisms that can be used as a source of natural bioactive compounds, one of which is Telescopium telescopium. T. telescopium until now it has not been yet exploited optimally, some people still use it as feed ingredients that contain high protein nutrition, however it must be supported by scientific information about the efficacy and side effects. This research aims at determining the secondary metabolite by crude extract T. telescopium, and the lethal toxicity value (LC50-24 hours). The research consisted of two steps, namely the phytochemical and toxicity test. The method used exploratif and experimental laboratory with a complete randomized design, which consited of five concentration treatments namely 25.12, 63.1, 158.5, 398.15, and 1000 ppm with three replications. Each treatment used 10 Artemia salina larvae, and observations were made during 24 hours of Artemia salina larvae mortality. LC50 was determined by probit analysis using the EPA Probit Analysis Program Version 1.5 Finney. The results of the phytochemical test showed that crude extract T. telescopium contain alkaloid compounds, steroids, flavonoids. The results BSLT (Brine Shrimp Lethality Test) of crude extract T. telescopium LC50-24 hours showed that the crude extract clorofom (229.562 ppm), crude extract ethyl acetate (244.906 ppm), and crude extract methanol (197.242 ppm). LC50-24 hours < 1000 ppm showed that the crude extract Telescopium telescopium potent antitumor compounds.
Optimasi Substrat Dan Pemilihan Co-Substrat Media Kultur Yang Sesuai Terhadap Pertumbuhan Dan Aktivitas Protease Ektraseluler Bakteri Bacillus Firmus Dari Perairan Nusa Lembongan Bali, Indonesia Muhammad Zainuddin; Delianis Pringgenies; Ocky Karna Radjasa; Haeruddin Haeruddin; Aninditia Sabdaningsih; Vivi Endar Herawati
Journal of Marine Research Vol 11, No 2 (2022): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v11i2.33278

Abstract

Udang merupakan sumber protein hewani yang banyak diminati masyarakat karena citarasa enak dan bergizi. Udang merupakan komoditas perikanan ekspor. Upaya pemenuhan kebutuhan ekspor udang saat ini telah dilakukan budidaya intensif. Budidaya intensif telah terjadi pemberian pakan yang berlebih sehingga terbentuk limbah organik di dasar tambak. Limbah pakan mengakibatkan terbentuknya senyawa nitrit dan amoniak yang toksik bagi udang. Komponen utama limbah ini adalah protein. Protein harus di dekomposisi menjadi asam amino oleh bakteri proteolitik. Salah satu bakteri yang memiliki aktivitas proteolitik adalah bakteri Bacillus Firmus dari simbion sponge Chalinula Pseudomolitba. Tujuan penelitian adalah melakukan optimasi substrat dan pemilihan co-substrat media kultur yang sesuai terhadap pertumbuhan dan aktivitas protease ektraseluler Bacillus Firmus. Penelitian menggunakan metode eksperimen laboratoris. Penelitian terdiri dari uji optimasi substrat, pemilihan Co-substrat C dan N. Hasil penelitian menunjukkan bahwa bakteri Bacillus firmus memiliki laju pertumbuah dan aktivitas protease terbaik pada media kultur dengan subtrat susu skim 1% dengan nilai 0,110 c/jamdan 12,566 c IU/ml. Sumber C terbaik adalah glukosa dengan nilai 0,124 c/jamdan 16,094 cIU/ml. Sumber N terbaik adalah amonium nitrat dengan nilai sebesar 0,137 c/jamdan 19,046 c IU/ml. Shrimp is a source of protein and have good taste. Shrimp is an export commodity. Shrimp cultivation is developing to be intensive. Intensive cultivation uses large amounts of feed. not all of the feed is eaten. leftover feed becomes waste. Feed waste produces nitrite and ammonia. Ammonia is toxic. Feed waste must be cleaned. Decomposition process by proteolytic bacteria. Bacillus Firmus is a proteolytic bacterium from the symbiont sponge Chalinula Pseudomolitba. The research aims to optimize the substrate and select the co-substrate. The study used a laboratory experimental method. The research consisted of a substrate optimization test, selection of Co-substrate C and N. The results showed that Bacillus Firmus had the best growth rate and protease activity on 1% skimmed milk substrate with values of 0.110 c/hour and 12,566 c IU/ml. The best source of C is glucose with a value of 0.124 c/hour and 16,094 c IU/ml. The best source of N as ammonium nitrate with values of 0.137 c/hour and 19,046 c IU/ml.
Co-Authors A. Sabdono ADI SANTOSO Agung Dhamar Syakti Agus Hardiyanto Agus Sabdono Agus Sabdono Agus Trianto Ali Ridlo Ambariyanto , Aninditia Sabdaningsih Antonius Budi Susanto Awang Surya Wiguna Awang Surya Wiguna, Awang Surya Bahry, Muhammad Syaifudien Bambang Yulianto Bayu Munandar Bintang Marhaeni Boedi Hendrarto Busaeri, Nundang Delianis Pringgenies Delianis Pringgenies Desy Wulan Triningsih Dietrich G Bengen Dietriech G. Bengen Dietriech Geoffrey Bengen Duhita Sinidhikaraning Kencana Dwi Haryo Ismunarti Elena Zocchi Endang Sri Lestari Endang Supriyantini Endang Supriyantini Fauziah Shahul Hamid Fera Nur Idawati Sahara Ferdy S. Rondonuwu Ferdy Semuel Rondonuwu Ferry Fredy Karwur Gede Iwan Setiabudi Gede Iwan Setiabudi Haeruddin Haeruddin Handung Nuryadi HANS- PETER GROSSART Hariyadi, Putut Hefni Effendi Hendi Perdian Yunianto Hendi Perdian Yunianto Herawati Sudoyo Hidetoshi Urakawa Hidetoshi Urakawal Ika Wulan Santi Ika Wulan Santi Iqna Kamila Abfa Irfan Dwidya Prijambada Isai Yusidharta Ita Riniatsih Ita Widowati JAKA WIDADA JOEDORO SOEDARSONO Johannes Hutabarat Kouichi Ohwada Kouichi Ohwadai Kumiko Kita-Tsukamoto Kumiko Kita-Tsukamotoz Leenawaty Limantara Lia Kusmita Lia Kusmita Mada Triandala Sibero Mada Triandala Sibero McCauley, Erin Meinhard Simon Meris Rahmawati Meutia Samira Ismet Miftahuddin M Khoeri Miftahuddin Majid Khoeri Misbakul Munir Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Mujizat Kawaroe Mukti K. Diana Putri Mutiara, Frista Nestin E.W. Ningrum Ni Komang Tri Utami Norma Afiati Nuning Vita Hidayati Nurhayati Nurhayati Nurkhasanah, Wulan Nurmanita Rismaningsih Nurul Hiron, Nurul Nurul Ria Arlita Paulus Damar Bayu Murti Pinandoyo Pinandoyo Prastyo Abi Widyananto Putut Har Riyadi R. Riyanti Rachmat Afriyanto Richardus F Kaswadji Rismaningsih, Nurmanita Riyanti Riyanti Riyanti Riyanti Riyanti, Rory Anthony Hutagalung Rr Citra Permata Rr Citra Permata Rr. Citra Permata Rr. Citra Permata Rudhi Pribadi Rudiger Stöhr S arjito S. Sulistiyani Saefuddin Aziz Sahala Hutabarat Sakti Imam Muchlisin Sakti Imam Muchlissin Sakti Imam Muchlissin Sarjito - Sekar Widyaningsih Septhy Kusuma Radjasa Seto Windarto Slamet B Prayitno slamet budi prayitno Slamet Budi Prayitno Sri Achadi Nugraheni Sri Sedjati Sri Yulina Wulandari Subagiyo Subagiyo Suharsono . Suhartono, Suhartono Supriharyono ,, Supriharyono Suyono ,, Suyono Suyono Suyono Tedi Septiadi Tonny Bachtiar TONNY BACHTIAR Tonny Bachtiar Tony Bachtiar Tony Bachtiar TORBEN MARTENS Tri Yuni Atmojo Tri Yuni Atmojo Uswatun Khasanah Vita Meylani Vivi Endar Herawati VIVI ENDAR HERAWATI Windarto, Seto Wiratno Wiratno Wismayanti, Gita Wittriansyah, Khoeruddin Wiwik Astuti Wiwik Astuti Wulan Nurkhasanah YS Darmanto Yustin Widyastuti