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Journal : Microbiology Indonesia

Modified Slide Culture Method for Faster and Easier Identification of Dermatophytes YEVA ROSANA; TETSUHIRO MATSUZAWA; TOHRU GONOI; ANIS KARUNIAWATI
Microbiology Indonesia Vol. 8 No. 3 (2014): September 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1069.613 KB) | DOI: 10.5454/mi.8.3.7

Abstract

Basic slide culture as a morphological identification was known as the most common method for the identification of pathogenic mold fungi. This method preserved the morphological features relatively undisturbed compared with adhesive tape preparations. However, it was necessary to modify method of basic slide culture to improve its usability and shorten the time it needed to identify mold fungi. There were four kinds of method carried out in this study; two kinds of modified slide culture, one kind of direct culture on slant agar plate, and a basic slide culture for identifying mold fungi, which result would be compared with each other. These four methods were tested to 4 species of dermatophytes which were known as mold fungi that could infect skin, hair, and nails in human; those were Trichophyton mentagrophytes, Microsporum canis, Microsporum gypseum, and Epidermophyton floccosum. Result of this study showed that both modified slide culture and direct culture on slant agar plate could visualize the structure of dermatophytes faster than basic slide culture method. These methods were also easier to prepare compared to basic culture method. Conclusion of this study showed that basic slide culture method needed to be modified for better identification of mold fungi.
Comparison of Microbial Pattern Causing Urinary Tract Infection in Female Out- and Hospitalized Patients in Jakarta YEVA ROSANA; DWIANA OCVIYANTI; ANIS KARUNIAWATI; SYADZA RHIZKY PUTRI AKHMAD
Microbiology Indonesia Vol. 10 No. 1 (2016): March 2016
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (493.251 KB) | DOI: 10.5454/mi.10.1.5

Abstract

Urinary Tract Infection (UTI) is an infection in any part of the urinary system. Women are 3 times more likely to have UTI than men. The UTI accounts for 15% infection cases in outpatients and 24% cases in hospitalized patients. Although the most common cause of UTI is certain bacteria, but it was not easy to choose the appropriate antimicrobial therapy. Strategy for choosing empiric antimicrobial treatments for UTI in female out- and hospitalized patients should be based on the pattern of the causative organisms. The aim of this study was to understand the microbial pattern causing UTI in female out- and hospitalized patients in Jakarta. The UTI -1 causative microorganisms were obtained from urine culture containing 100,000 cfu/mL . Twenty nine microorganisms were found as the causative agents of UTI in 317 pregnant women who came to six Community Health Centres (Puskesmas) in Jakarta: Makassar; Pulogadung, Cakung, Pasar Rebo, Duren Sawit, and Kramat Jati for antenatal care. Twenty nine microorganisms were isolated from 114 urine samples of female hospitalized patients who were diagnosed of UTI. The samples were obtained from the Microbiology Laboratory Clinic of FKUI-RSCM. The most common microorganisms causing UTI in female out- and hospitalized patients were Gram negative bacteria. In female outpatients, Klebsiella sp was the most common causative bacteria (31%), followed by Escherichia coli (24.1%). In female hospitalized patients, Escherichia coli was the most common causative bacteria (30%), followed by Candida sp (24.1%) and Klebsiella pneumonia (6.8%). There was more variation in the pattern of UTI causative organisms in hospitalized female patients in comparison to that of the outpatients. Candida sp. was only found in hospitalized UTI patients but not in outpatients.
The qPCR Assay for Detecting The Presence and Relative Abundance of Pseudomonas aerugionosa and Antibiotic Resistance Gene aadA2 in Hospital Wastewater of National Reference Hospital Dr. Cipto Mangunkusumo (RSCM) Rida Tiffarent; Rosdiana Irawati; Conny Riana Tjampakasari; Fithriyah Sjatha; Windi Muziasari; Anis Karuniawati
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (117.977 KB) | DOI: 10.5454/mi.16.2.24-30

Abstract

Antimicrobial resistance is one of the top 10 global health threats. The hospital wastewater (HWW) potentially becomes the reservoir and dissemination of antibiotic resistance gene (ARG) and bacterial pathogens. In Indonesia, the protocol to monitor the ARGs form HWW has not been established. This study aimed to detect the presence and find the relative abundance of P. aeruginosa and aadA2 genes from Dr. RSUPN. Cipto Mangungkusumo (RSCM) inlet and outlet wastewater through qPCR assay. The primers used were supported by Resistomap. The study revealed that the qPCR assay was able to detect the Ct value of P. aeruginosa and aadA2. The aadA2 gene was found in all waste water samples, meanwhile P. aeruginosa was only found in some of inlet samples. aadA2 had the highest relative abundance and this gene’s mobility uses plasmids and integrons that potentially enhance the acquired antimicrobial resistance (AMR) mechanism. This study implicated that qPCR assay was capable to detect pathogenic bacteria and ARG, and ARG could be released to the environment even though the wastewater samples have been proceeded in wastewater treatment plants (WWTP). The qPCR assay can be used as the method to monitor the AMR status in a hospital and the spreading potency to the environment using the HWW.
Co-Authors . Andriansjah Ade Dharmawan Amalia Almira Amarila Malik Amin Soebandrio Andi Annisa Rusyda Khafiyani Andi Yasmon Andra Aswar Andra Aswar, Andra Angky Budianti Anwar S. Ibrahim Aprilia Rakhmawati Ardiana Kusumaningrum Arfan, Ahmad Ari Fahrial Syam Ari Prayitno Aria Kekalih Aryo Tedjo Asmarani Kusumawati Asmarani Kusumawati Augustine Natasha Aulia Rizki Bachti Alisyahbana Badriul Hegar Badriul Hegar Bambang Madiyono Budi Haryanto Burhanuddin Iskandar Ceva W. Pitoyo Chairunissa T. Rizal Cleopas Martin Rumende Cleopas Martin Rumende Delly Chipta Lestari Delly Chipta Lestari Delly Chipta Lestari Delly Chipta Lestari Delly Chipta Lestari Dimas Seto Prasetyo Dwiana Ocviyanti E. Risdiyani Em Yunir Em Yunir, Em Erwin Christian Susanto Esthika Dewiasty Esthika Dewiasty, Esthika Falasiva, Rezyta Fariz Nurwidya Febrianti, Tati Febriyana, Dwi Fitriah, Laela Futihati Ruhama Zulfa Gestina Aliska Gortap Sihotang Hana Paraswati Putri Handaya, Adeodatus Y. Hasanah, Lili Hindra Irawan Satari Hindra Irawan Satari Ida Parwati Ika Ningsih Indah S. Widyahening Iswanto, J. Joyce Bratanata Joyce Bratanata, Joyce Kambang Sariadji Khie Chen Kuntjoro Harimurti Lalisang, Toar JM. Linosefa Linosefa Manuel Lamberto Willem Mboeik Martin Hartiningsih Mboeik, Manuel Lamberto Willem Moehario, Lucky Hartati Muhammad K Azwar, Muhammad K Mulya Rahma Karyanti, Mulya Rahma Mulyadi M. Djer Muziasari, Windi Nabila Maudy Salma Nadia Maretti Nasution, Safruddin Nelly Puspandari Nie Nie Nina Dwi Putri Nuraini I Susanti Permatasari, Tri Wijayanti Pratama Wicaksana Pratiwi Pujilestari Sudarmono Pratiwi Sudharmono Pratiwi Sudharmono, Pratiwi Prawoto Prawoto Prawoto Prawoto Pujilestari, Ratih Purwantyastuti Purwantyastuti Puspandasari, Nelly Rakhmawati, Aprilia Resti H. Lestari Reynaldo Reynaldo Reynaldo Reynaldo, Reynaldo Rezyta Falasiva Riamin Sitorus Rianto Setiabudi, Rianto Rida Tiffarent Rosdiana Irawati Rudyanto Sedono Rudyanto Sedono Rudyanto Sedono, Rudyanto S. Nilawati Santya Fatma Dewi Sari Wiraswasty Sari, Rifta Saunar, Rofy S. Shindy Claudya Aprianti Simanjuntak, Glory Gelarich Sitorus, Truly Panca SJATHA, FITHRIYAH Subari A. Riyanto Sudigdo Sastroasmoro Sukman T. Putra Sunarno Sunarno Susan M. Noor Susan M. Noor, Susan Susan Maphilindawati Noor Susanti, Nuraini I Susanti, Nuraini I Susanto, Erwin Christian Sweety Pribadi SYADZA RHIZKY PUTRI AKHMAD T. M. Sudiro Teguh Harjono Karjadi Teguh Harjono Karjadi, Teguh Harjono TETSUHIRO MATSUZAWA Thomas Robertus Tjampakasari, Conny Riana TOHRU GONOI Tony Loho Trisni U Dewi, Trisni U Truely Panca Sitorus Usman, Nurhayat Weaver, Tom Wia Melia Windi Muziasari Yefta Moenadjat Yeva Rosana Yulia R. Saharman Yulia Rosa Saharman