Article Per Year (5 Year)
p-Index From 2021 - 2026
5.059
P-Index
This Author published in this journals
All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Jurnal Akademika Biologi International Journal of Marine and Aquatic Resource Conservation and Co-existence Biotropika Jurnal Kelautan Tropis E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology Scripta Biologica Cendekia Journal of Pharmacy Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia Berita Biologi
Hermin Pancasakti Kusumaningrum
Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

Published : 62 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 62 Documents
Search

 3   4   5   6   7 
Antioxidant Activity of Red Ginger Kombucha (Zingiber officinale var. rubrum) with Various Fermentation Time and Palm Sugar Concentrations Choirunnisaa, Nadia Maharani Jasmine; Jannah, Siti Nur; Lunggani, Arina Tri; Pujiyanto, Sri; Kusumaningrum, Hermin Pancasakti
Journal of Applied Food Technology Vol 12, No 1 (2025)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17728/jaft.23277

Abstract

The use of red ginger (Zingiber officinale var. rubrum) as the primary ingredient of kombucha is a diversification to increase the antioxidant activity of kombucha. The presence of gingerol dan shogaol in red ginger acts as a potential antioxidant, while the use of palm sugar serves as an additional source of antioxidants and alternative source of sucrose from regular granulated sugar. The fermentation time and the amount of sugar used are two variables that affect the kombucha’s fermentation process. This study aims to examine the effect of various fermentation times and palm sugar concentrations on the antioxidant activity, pH, alcohol content, and organoleptic properties of red ginger kombucha. The methods used were antioxidant test with DPPH, pH test, alcohol content, and organoleptic test with 20 panelist. The results indicate that variations in fermentation time and concentration of palm sugar have a significanr effect (α = 0.05) on the antioxidant activity with IC50 values ranging from 27.89 ppm to 46.77 ppm, categorized as very strong antioxidant activity. The characteristics of red ginger kombucha includes pH 3.6 to 4.6, alcohol content 0.2% to 0.3%, and organoleptic properties varying from moderately liked to liked. Red ginger kombucha fermented for 12 days with 20% palm sugar showed an IC50 value of 40.28 ppm, which categorized into very strong antioxidant activity and is most preferred by the panelists with organoleptic scores of color attribute at 4, aroma at 3.65, and taste at 4.
Deteksi Gen DXS dan Penentuan Jalur Biosintesis Karotenoid pada Chlorella pyrenoidosa Monalita, Ramadhebi; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Berkala Bioteknologi Vol. 5, No. 1, April 2022
Publisher : Berkala Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Natural carotenoid synthesis has never exceeded synthetic products on a commercial scale. Lack of understanding of the microbiological and ecophysiological aspects of carotenoid-producing isolates leads to misidentification of species. One local isolate of green algae is used as a natural food source of carotenoids in the food industry, namely Chlorella pyrenoidosa. Carotenoid accumulation of the nonMVA pathway in green algae is determined by the enzyme D-1-deoxyxylulose 5-phosphate synthase, which is encoded by the D-1-deoxyxylulose 5-phosphate synthase (DXS) gene. The main purpose of this study was to detect the DXS gene as a carotenoid biosynthetic key enzyme encoder in C. pyrenoidosa whether or not to follow the non-MVA pathway for carotenoid biosynthesis or not. Morphological and ecophysiological characterization methods are carried out based on periodic observations and DXS gene detection using the guide Kuzuyama (2000). The results of the analysis of the similarity of the C. pyrenoidosa DXS gene in sustainable areas show that it can detect partial DXS genes from Chlamydomonas reinhardtii. The absence of growth inhibition in C. pyrenoidosa with lovastatin shows a non-MVA pathway that is the pathway used in carotenoid biosynthesis.
Analisis SNP (Single Nucleotide Polymorphism) Promotor GEN IL-10 pada Penderita Filariasis Maria, Atina; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti
Berkala Bioteknologi Vol. 4, No. 2, November 2021
Publisher : Berkala Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Filariasis limfatik merupakan suatu penyakit yang disebabkan oleh nematoda dari jenis Wuchereria bancrofti dan Brugia malayi dewasa yang menghuni jaringan limfatik. Filariasis limfatik ini disebarkan oleh empat genus nyamuk, yaitu, Anopheles, Culex, Aedes dan Mansonia. Respon imun terhadap parasit filaria terdiri dari Thelper2 (Th2) dan melibatkan produksi cytokin –IL-4, IL-5, IL-9, IL-10, dan IL-13, isotype antibody—IgG1, IgG4 and IgE, dan meningkatnya populasi eosinophils dan makrofag yang aktif. Polimorfisme pada daerah promotor gen IL-10 diidentifikasi berhubungan dengan tinggi rendahnya produksi IL-10 pada sel darah perifer pada stimulasi in vitro dengan lipopolysacharide (LPS). Sampel diambil dari penderita filariasis, kemudian diekstraksi dan diamplifikasi pada posisi -1082, -819, -592. Hasil amplifikasi kemudian di restriksi dan di sekuensing. Hasil penelitian dari sampel penderita filariasis yang diteliti menunjukan memiliki haplotip ACA pada analisis RFLP. Analisis hasil sekuensing menggunakan dua aplikasi, yaitu DNA baser dan Bioedit. Analisis menggunakan DNA baser dan Bioedit serta dibandingkan dengan tag SNP didapatkan satu SNP. Perbandingan antara sekuens DNA yang didapatkan dengan tag SNP mandapatkan haplotip CC.
EKSPRESI GEN PENYANDI PEROKSIDASE CABAI MERAH (Capsicum annuum L.) (CaPer) SEBAGAI RESPONS TERHADAP Fusarium oxysporum Elsima, Athried; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Berkala Bioteknologi Vol. 6, No. 1, April 2023
Publisher : Berkala Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Cabai merah (Capsicum annuum L.) merupakan komoditas pertanian penting di Indonesia yang sering terkena serangan OPT ( organisme pengganggu tanaman) misalnya Fusarium oxysporum. Tanaman cabai merah memiliki sifat ketahanan kimiawi aktif yaitu mengekspresikan peroksidase sebagai respons terhadap infeksi patogen. Penelitian ini bertujuan untuk mengetahui ekspresi gen penyandi peroksidase pada tanaman cabai merah sebagai respons terhadap jamur F. oxysporum. Penelitian ini diawali dengan inokulasi jamur pada tanaman cabai merah dilakukan dengan metode rendaman akar. Analisis ekspresi gen penyandi peroksidase menggunakan qRT-PCR dilakukan pada gen CaPer dengan gen 18s rRNA sebagai gen pembaku, pada interval 6, 48, dan 96 jam setelah inokulasi. Hasil penelitian gen CaPer terekspresi paling tinggi pada 6 jam setelah inokulasi.
Pemberdayaan UMKM Atsiri Desa Selokarto Batang dalam Pembuatan Cat Aromaterapi Antimikroba dan Aplikasinya Kusumaningrum, Hermin Pancasakti; Hersugondo, Hersugondo; Zainuri, Muhammad; Sugiarti, Lilis
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 15, No 4 (2024): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v15i4.20440

Abstract

Tanaman atsiri merupakan unggulan ciri khas Kabupaten Batang dimana produk minyak atsirinya menempati peringkat ke 3 di Indonesia dan utamanya terdiri dari minyak cengkeh, nilam, dan serai wangi. Minyak atsiri Indonesia mempunyai pangsa pasar lebih dari 90% kebutuhan dunia atau sekitar 35-40% dari total nilai ekspor minyak atsiri. Kabupaten Batang memiliki sekitar 30 UMKM yang bekerja secara sinergis di bidang wirausaha produksi dan pengolahan minyak atsiri. Meskipun demikian, harga minyak atsiri sangat mudah naik turun bergantung pada pasar. Hal ini menyebabkan perlunya upaya diversifikasi berbagai produk berbasis atsiri yang dapat dikerjakan oleh UMKM (Usaha Menengah Kecil dan Mikro) dan Kelompok Wanita Tani (KWT)  dan diversifikasi produk berbasis atsiri yang mempunyai nilai ekonomi cukup tinggi. Pengabdian Masyarakat yang dilakukan bertujuan melatih pembuatan diversifikasi produk berbasis atsiri.  Kegiatan dilakukan melalui pelatihan diversifikasi produk atsiri berupa pembuatan bahan tambahan cat aromaterapi antimikroba. Hasil kegiatan memperlihatkan UMKM dan KWT mengerti dan mampu membuat produk yang disuluhkan dengan baik dan bernilai ekonomi. Selain itu mereka juga sangat tertarik untuk mengaplikasikan cat aromaterapi antimikroba di lingkungan mereka agar tercipta lingkungan yang lebih sehat.
MOLECULAR IDENTIFICATION AND MORPHOLOGICAL CHARACTERIZATION OF PATCHOULI (Pogostemon sp.) FROM BATANG REGENCY, CENTRAL JAVA PROVINCE Kusumaningrum, Hermin Pancasakti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 9 No. 1 (2022)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2022.1787

Abstract

Patchouli (Pogostemon cablin Benth.) is an essential oil-producing plant from Batang Regency that has excellence in patchouli alcohol contents and highly survives in any condition. Molecular identification has been done in the ITS region because DNA sequences in the ITS rRNA region evolved faster than in other areas. This study aimed to get the molecular and morphological identity of patchouli (Pogostemon sp.) from BPP Kabupaten Batang. The study consisted of sample preparation, DNA isolation, amplification, electrophoresis, sequence data analysis, and phylogenetic analysis using MEGA X. All parts of plant were morphologically identified and compared with patchouli from Sidikalang, Aceh, Java, and China. Extraction DNA produced 301.7 ng ?L–1 concentration and 1.93 purity. Amplification of ITS fragment patchouli produced a 670 bp-sized single band. Phylogenetic analysis showed patchouli BPP related to Pogostemon cablin (KR608752.1) with 98% coverage identity. BPP patchouli showed 62,5% morphological similarity with Sidikalang patchouli compared to Java patchouli. In conclusion, BPP patchouli is a Sidakalang patchouli P. cablin that has undergone environmental adaptation.
MOLECULAR CHARACTERIZATION, PHYTOCHEMICALS SCREENING, AND MOLECULAR DOCKING OF CARDAMOM (Wurfbainia compacta), AND SAMBILOTO (Andrographis paniculata) AGAINST COVID-19 Kurniawati, Mufida Budi; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3005

Abstract

Cardamom and Sambiloto are phytopharmaceutical plants that produce phytochemical compounds that have the potential to be used to increase immunity against COVID-19. because they contain carotenoids, phenols, anthocyanins, saponins, alkaloids and steroids. This research aims to obtain the molecular characteristics of Cardamom and Sambiloto plants from the Gunungpati area, Semarang by ITS primer, testing phenolic phytochemicals, tannins, flavonoids, saponins and alkaloids followed by molecular docking tests with the 6WX4 protein. SARS-CoV-2. Molecular characterization results show that Cardamom and Sambiloto are similar to Wurfbainia compacta MF802556.1 (100%) and Andrographis paniculate LC646073.1 (84.47%). The results of the phytochemical test screening showed that both plants contain flavonoids. Molecular docking tests were carried out with the compounds Quercetin, Avicularin, Naringenin, 5-hydroxy-7,8,2',5' tetramethoxyflavone, and Retinoic Acid. Retinoic Acid as a test ligand has the greatest potential in inhibiting the 6WX4 protein in the SARS-CoV-2 virus with a binding affinity value of -7.28 and RMSD 0.00.
SALTED FISH PRESERVATION MENGGUNAKAN HERBS EDIBLE COATING DARI NANOPARTIKEL BAWANG PUTIH, DAUN PANDAN DAN SERAI DAPUR Doktorasaintifika, Heradita Kaniaazzahra; Nurkertamanda, Denny; Shasabila, Analies Devi; Kusumaningrum, Hermin Pancasakti; Sugiarti, Lilis; Wahyuningsih, Candra; Herida, Azalia Puspa; Hapsari, Hererapratita Aysha
Cendekia Journal of Pharmacy Vol 9, No 3 (2025): Cendekia Journal of Pharmacy
Publisher : Institut Teknologi Kesehatan Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31596/cjp.v9i3.385

Abstract

Global consumption trends show a tendency to choose natural food preservatives, especially those derived from plants, especially to avoid chemical and synthetic preservatives that are harmful to health. Nanotechnology is an important development and an efficient choice to extend the shelf life of food. This study aims to create and carry out seafood preservation using herbs edible coating from garlic nanoparticles, pandan leaves and lemongrass to determine its use as a preservative for salted mackerel and tigawaja salted fish. As a comparison, a mixture of the three ingredients. The study used four treatments where each was repeated 3 times. A hedonic test was also carried out in the form of observations of dryness, color, texture and odor of salted fish. The research method consisted of making garlic nanoparticle extract, pandan leaf extract, lemongrass extract, and a mixture of the three ingredients followed by microscopic observation. Antibacterial activity was carried out using the TPC test. The hedonic test was carried out to see the potential of consumers for the product. Furthermore, a PASS analysis was carried out to see the potential of antimicrobial compounds in the three ingredients. The results showed that the edible coating layer was made into nanoparticle form. Edible coating from pandan is most suitable for use on salted mackerel. Edible coating from garlic is most suitable for use on tigawaja salted fish. The TPC test results showed that garlic, pandan and lemongrass were able to prevent microbial growth effectively for 48-96hours and have the potential to be used as edible coating.
KKN Tematik: Pemberdayaan KWT melalui Aplikasi TTG Produk Sabun Pembersih Lantai berbantuan Atsiri Hutan dan Eco-enzyme di KHDTK Wanadipa Undip dan E-commerce Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Ngestiningsih, Dwi; Isnaeni, Aissya Safriliana; Razana, Aqila Khaira; Alfiatuzzahra, Diva; Kurniantoro, Vangga Deswanto Prasetyo; Badzlin, Zahrah Syahmina; Maulidya, Shaula Dzikra; Hidayati, Bintang Nurul; Ardan, Kanaya Chandra Ajeng Sotya; Pasha, Muhammad Affan; Maharani, Prabaswara Nasywa
E-Dimas: Jurnal Pengabdian kepada Masyarakat Vol 17, No 1 (2026): E-DIMAS
Publisher : Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/e-dimas.v17i1.25024

Abstract

Ketersediaan sumber daya alam yang melimpah di KHDTK Wanadipa Undip, khususnya tanaman penghasil atsiri belum banyak dimanfaatkan. Kelompok Wanita Tani (KWT) mengalami kesulitan dalam memanfaatkan tanaman atsiri untuk memnghasilkan produk khas hutan yang berkualitas dan bernilai jual tinggi. Kegiatan bertujuan memberikan aplikasi TTG melalui pelatihan pembuatan produk  sabun pembersih lantai berbantuan atsiri hutan dan Eco-enzyme yang mempunyai nilai ekonomi beserta teknik pemasarannya melalui e-commerce. Pengabdian dilakukan dengan metode observasi, dan pelatihan langsung kepada anggota KWT. Hasil yang didapatkan yaitu KWT dapat menghasilkan sabun pembersih lantai atsiri beraroma atsiri hutan dan berbantuan Eco-enzyme secara mandiri yang bernilai jual dan peningkatan keterampilan dalam pemasaran melalui platform e-commerce. Kegiatan yang dilakukan telah terbukti meningkatkan kesejahteraan ekonomi masyarakat dan KWT di sekitar KHDTK Wanadipa Undip secara berkelanjutan.
Exploration of Copper and Lead Resistant Bacteria from Sediments of Kali Baru Estuary Purnomo, Eko; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Lunggani, Arina Tri; Az-Zahra, Salwa Zubaidah; Wardaja, Bodhicitta; Maimunah, Siti; Dinalhaq, Putri Cahya
Biotropika: Journal of Tropical Biology Vol. 14 No. 1 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.01.05

Abstract

Heavy metal contamination, particularly by copper (Cu) and lead (Pb), in aquatic ecosystems poses a significant threat to ecological balance, aquatic organisms, microbial diversity and human health. The Kali Baru Estuary in Semarang, Indonesia, has been reported as a heavily polluted area with Cu and Pb concentrations far exceeding environmental safety thresholds, highlighting the urgent need for sustainable remediation strategies. This study aimed to isolate and characterise bacterial strains resistant to Cu and Pb from sediment samples collected from the Kali Baru Estuary in Semarang as potential bioremediation agents. Bacterial isolation was performed using a serial dilution technique up to 10?², in triplicate, spread onto Marine Agar medium, and incubated at 37°C for 24 h. The obtained isolates were purified and characterised based on their macroscopic and microscopic morphological features. Resistance profiling of the isolates was assessed by exposing them to Cu and Pb at concentrations of 100, 200, and 300 mg/L. A total of 32 bacterial isolates were successfully obtained. Among these, two isolates demonstrated resistance to Cu, and 14 isolates exhibited resistance to Pb at the highest concentration tested (300 mg/L). These metal-resistant bacterial isolates exhibit substantial promise for bioremediation applications aimed at remediating Cu and Pb-contaminated aquatic environments.
Co-Authors Agung Suprihadi Agung Suprihadi Agus Sabdono Agus Setiadi Agus Subagio Alfiatuzzahra, Diva Anggoro, Naufal Sebastian Annisa Fadillah Annisa Nur Ayuningtyas Anto Budiharjo Anto Budiharjo Ardan, Kanaya Chandra Ajeng Sotya Arina Tri Lunggani Arina Tri Lunggani Aris Ismanto Asih Rismiarti, Asih Athried Elsima Az-Zahra, Salwa Zubaidah Azis Rifai Badzlin, Zahrah Syahmina Baskoro Rochaddi Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Chatarina Umbul Wahyuni Choirunnisaa, Nadia Maharani Jasmine Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Clara Yully Diana Ekaristi Denis Denny Nugroho Sugianto Denny Nurkertamanda Dinalhaq, Putri Cahya Dinda Khairunnisa, Dinda Djuara P Lubis Doktorasaintifika, Heradita Kaniaazzahra Dudi Hardianto, Dudi Eiffeliena Nur'aini Fisikaningputri Purwienanti Eko Purnomo Eko Purnomo Elke Gildantia Elsima, Athried Endang Dwi Purbajanti Endang Kusdiyantini Erfianti, Tia Fiona Aqhila Dewi Fitri Arum Sasi, Fitri Arum Furgeva, Natasha Gentur Handoyo Hadi Endrawati Hapsari, Hererapratita Aysha Heksa Raihan Hererapratita Aysha Hapsari Herida, Azalia Puspa Hersugondo Hersugondo Herusugondo Herusugondo Hidayati, Bintang Nurul Imam Misbach Imam Mishbach Indah Sulistyarini Indras Marhaendrajaya Is Helianti IS HELIANTI Isnaeni, Aissya Safriliana Istiana, Rohma Ita Widowati Izzudin, Maulana Zidan Jihadi, Muhammad Shulhan Joedono Soedarsono Joedoro Sudarsono Karima, Radhita Kurniantoro, Vangga Deswanto Prasetyo Kurniawati, Mufida Budi Lilis Sugiarti Lilis Sugiarti Lina Mulyawati Maharani, Prabaswara Nasywa Maria, Atina Marissa Angelina Maulidya, Shaula Dzikra Mawarni, Shelina Nurunnisa Maya Aresteria Meliana, Sekar MG Isworo Rukmi Monalita, Ramadhebi Muhammad Amal Nurhakim, Muhammad Amal Muhammad Anwar Djaelani Muhammad Iskandar Zulkarnain Muhammad Iskandar Zulkarnain Muhammad Mulyadi, Muhammad Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Nurhayati Nurhayati Okti Hajeng Kristiadi Pasha, Muhammad Affan Rahayu, Annisa Rizky Razana, Aqila Khaira Rejeki Siti Ferniah Retno Hartati Risma Wiharyanti Rizko, Nurmalisa Robertus Triaji Mahendrajaya Roslenawati Roslenawati Rudhi Pribadi Safirah, Dearesty Sarwo Edi Wibowo, Sarwo Edi Shasabila, Analies Devi Siti Maimunah Siti Nur Jannah Siti Nur Jannah Solly Aryza Sonny Abdi Setiyawan, Sonny Abdi Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Rustini Subagiyo Subagiyo Sulis Setyowati, Sulis Suryani, Oda Gracia Ariela Sutrisno Anggoro Tony Hadibarata, Tony Tri Rahayu, Hesti Triwibowo Yuwono Wahyu Dewi Utari Haryanti Wahyuningsih, Candra Wardaja, Bodhicitta Widianingsih Widianingsih Wijanarka Wijanarka Yann Hardivillier Yason Lukman Sudjito, Yason Lukman Yosef Purwoko Yoshua Mario Sumbodo Yudi Yunanto Yumna Rahmadias Hanifa Yunior William Susanto Yuvita Muliastuti