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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Jurnal Akademika Biologi International Journal of Marine and Aquatic Resource Conservation and Co-existence Prosiding KPSDA Jurnal Kelautan Tropis E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology Scripta Biologica Cendekia Journal of Pharmacy Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Hermin Pancasakti Kusumaningrum
Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University
Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Prediksi Resistensi Udang Vaname (Litopenaus vannamei) terhadap Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV) dari Tambak Intensif dan Semi Intensif Jepara Menggunakan Marka RAPD Mulyadi, Muhammad; Handayani, Christina Ratna; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (91.931 KB) | DOI: 10.14710/bioma.15.2.73-80

Abstract

Infectious Hypodermal and Hematopoietic Necrosis Infectious Virus (IHHNV) is the most important DNA virus which can lead to Runt Deformity Syndrome (RDS) in vaname shrimp. The RAPD technique can be used to determine the resistance of a species to a disease. This research aimed to screen and identify RAPD markers which could distinguish the resistance of vaname shrimp to IHHNV reared at intensive and semi-intensive pond. The DNA template was amplified by PCR using 5 primers : OPA 06, OPA 08, OPA 19, OPD-02 and OPZ-15. The results showed that only the primer OPA-19 and OPZ-15 were able to produce 100% polymorphic bands with sizes from 400-1700 bp as well as showing the resistance IHHNV in vaname shrimp. Based on these results, vaname shrimp which reared at the intensive pond were more resistant to IHHNV compared with the semi-intensive pond.   Key words: RAPD, shrimp vaname, IHHNV, intensive & semi-intensive pond  
Aplikasi Pakan Kaya Karotenoid Hasil Fusi ProtoplasmIntergenera Dunaliella salina dan Chlorella vulgaris pada Udang Windu (Penaeus monodon F.) Stadia PL-20 Di Desa Asempapan, Pati, Jawa Tengah Rahmawati, Novia; Zainuri, Muhammad; Kusumaningrum, Hermin Pancasakti
Bioma : Berkala Ilmiah Biologi Vol. 15, No.2, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (799.549 KB) | DOI: 10.14710/bioma.15.2.46-52

Abstract

Dunaliella salina and Chlorella vulgaris is a natural feed microalgae with high carotenoid content that can be increased using protoplast fusion technique. Protoplast fusion as one of the application fields of genetic engineering is a method for obtaining recombinant with the desired properties and profitable in a short time. This study aimed to see the effect of the addition of carotenoid-rich feed results from protoplast fusion recombinant D. salina and C. vulgaris on the survival rate and weight of shrimp post larvae. Mixed fusion results feed and artificial feed needed for the growth of post-larval shrimp, moulting and skin pigmentation. The results showed that the recombinant from protoplast fusion intergenera D. salina and C. vulgaris contains carotenoid pigments higher, reaching 124.6 mg / g bks from the second parent, namely D. salina reached 101.83 mg / g bks, while C. vulgaris 97.18 ug / g bks. Feed manufacturing is done by mixing pellets and 80-100x103 cells per 0.0225 g of feed. Application of feed carried on Penaeus monodon F. (tiger prawn) stadia PL-20 for a month. The results of weight measurements showed the highest prawn post larvae reached at artificial feeding plus recombinant protoplast fusion results intergenera D. salina and C. vulgaris and was able to raise the level of post-larval shrimp survival rate reached 88%.   Keywords: D. salina, C. vulgaris, Protoplast Fusion, Carotenoid, Penaeus monodon F.
Pelacakan Gen Sitokrom Oksidase Sub Unit I (COI) DNA Mitokondria Itik Tegal (Anas domesticus) Menggunakan Primer Universal Wibowo, Sarwo Edi; Djaelani, Muhammad Anwar; Kusumaningrum, Hermin Pancasakti
Bioma : Berkala Ilmiah Biologi Vol. 15, No.1, Tahun 2013
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (244.965 KB) | DOI: 10.14710/bioma.15.1.20-26

Abstract

Currently local ducks are generally quite difficult to find in a big farm in Inonesia, including Tegal ducks. Tegal ducks is one of the genetic resources native to Indonesia with it’s advantages in terms of high and large egg production. Conservation and development of local ducks have strived to maintain our existence of Indonesian livestock germplasm. If such information is not superior to native species exist, the opportunity to increase his lead further also getting smaller. Tracking the mitochondrial COI gene DNA of Tegal ducks may underlie the process of an organism's genetic characterization. Information about Tegal duck mitochondrial DNA has not been done. The information obtained can be used for optimization of duck products native to Indonesia both in physiological aspects, phylogeny and genetic engineering. The research method used in this research is tracking COI gene data from Gen Bank with the programs Clustal X and Genedoc. Tracking then continued using universal primers HCO and LCO. The results of the data followed up with the isolation and amplification of COI gene mitochondrial DNA as well as the optimization of PCR conditions. The results showed mitochondrial DNA COI gene Tegal ducks were amplified with primer LCO obtain DNA fragments of length less than 250 bp. Kata kunci: duck’s from Tegal, COI gene, mitochondrial DNA
Kloning dan Sekuensing Gen Xilanase dengan Produk Gen Berukuran 30 kDa dari Bacillus halodurans CM1 pada Escherichia coli DH5α Safirah, Dearesty; Helianti, Is; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto
Bioma : Berkala Ilmiah Biologi Vol. 18, No.2, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (98.71 KB) | DOI: 10.14710/bioma.18.2.167-172

Abstract

The paper industry contributed the environment pollution due to chlor substances. Utilization of alkalothermophilic xylanase enzyme as a biocatalyst in the production of paper may become an environmentally friendly biobleaching alternative. Bacillus halodurans CM1 produces xilanase enzyme that had optimal activity at pH 9 and temperature 70°C. Previous study showed that this CM1 strains has several xilanase genes. The cloning of one of these alkalothermophiic xylanase (alkxyn) gene has been already conducted. This study aimed to clone alkxyn gene that encode alkalothermophilic xylanase enzyme from B. halodurans CM1 into Escherichia coli DH5α. Amplification of alkxyn has been carried out using primers for amplification xylanase 30 kDa. The alkxyn gene fragment was inserted into pGEM-T Easy vector and then transformed into E. coli DH5α. The results showed that the recombinant of E. coli DH5α harboring alkxyn gene from B. halodurans CM1 has been obtained. The sequences analysist based on BLAST showed that alkxyn fragment has homology (99%) with the alkaliphilic xylanase gene from Bacillus sp. 31 which encodes alkaliphilic xilanase (Genebank assession number: JF912895.1). Keywords: cloning, Bacillus halodurans CM1, xylanase, alkalothermophilic.
Karakterisasi Dan Identifikasi Molekuler Fusan Hasil Fusi Protoplas Interspesies Chlorella pyrenoidosa dan Chlorella vulgaris Menggunakan 18SrDNA Rismiarti, Asih; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad
Bioma : Berkala Ilmiah Biologi Vol. 18, No.1, Tahun 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (277.911 KB) | DOI: 10.14710/bioma.18.2.30-40

Abstract

Chlorella pyrenoidosa is a unicellular green algae that grows in fresh water with carotenoids consisting of             β-carotene, α-carotene, anthaxanthin, neoxanthin, zeaxanthin and lutein. C. vulgaris usually it lives in sea water with carotenoids, chlorophyll, tocopherol, ubiquinone and proteins. The quality of them is improved by protoplast fusion and identified further using moleculer analysis. This study aims to find out the characterization and identification of molecular fusan that is obtained from interspecies C. pyrenoidosa and C. vulgaris protoplast fusion process using 18SrDNA. Both C. pyrenoidosa and C. vulgaris are combined by protoplast fusion and then they were performed the isolation of DNA with CTAB modification method, followed by PCR gradient using primers 18S Chlorella and performed DNA sequencing. The result show that there are different characters between masterplan and fusan based on growth of fresh water and sea water medium. The success frequency of fusan as a result from protoplast fusion in the fresh water media is 21% and 6% for sea water medium. The results of the alignment between fresh water fusan and C. vulgaris masterplan from GeneBank shows that the base sequence homology is 93% C. pyrenoidosa masterplan from GeneBank is 90%. The result of molecular identification towards the sequence of fresh water fusan bases shows that there is a kinship relationship with the masterplan of C. pyrenoidosa 18S Chlorella and Chlorosphaera klebsii microalgae compared with some other species from Chlorophyta group with similarity value as many as 91%. It shows that the high variety genetic is based on variations of the base sequence and has a kinship with other species in the Chlorophyta group.                                      Keywords : Chlorella pyrenoidosa, Chlorella vulgaris, Protoplast Fusion, DNA Sequensing, 18SrDNA.
Fusi Protoplas Interspesies Chlorella pyrenoidosa dan Dunaliella salina Yunanto, Yudi; Kusumaningrum, Hermin Pancasakti; Pujiyanto, Sri
JURNAL SAINS DAN MATEMATIKA Volume 21 Issue 1 Year 2013
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3993.052 KB)

Abstract

Microalgae Chlorella pyrenoidosa and Dunaliella salina have been used as natural aquaculture food supplement because the previous contains 60,5% proteins and 180,8 mg/100g β-carotene and the other  were accumulated β-carotene by 95% from their total carotenoid.  Carotenoid  production can be improved by protoplast fusion technique. The aim of the research was conducted protoplast fusion of from C. pyrenoidosa and D. salina in order to gaining boarder salinity spectrum for natural aquaculture food supplement . The research metodology consist of protoplast  isolation followed by protoplast fusion process  induced by PEG6000 and regeneration of  fusant.  Protoplast fusion was done in three different PEG incubation time that are 15, 30 and 45 minutes.  The fusants were grown in 2 different medium, sea water media and fresh water media.  Research result shows that optimal fusion incubation time with PEG6000 is at 30 minutes.  Fusant can grown in both  medium and revealed higher β-carotene contents 2,008 µg/ml comparing with their parents.   Keywords: Protoplast fusion, Chlorella pyrenoidosa, Dunaliella salina, β-carotene.
Analisis Kandungan Kadmium (Cd) dalam Tanaman Bawang Merah dari Tegal Kusumaningrum, Hermin Pancasakti; Herusugondo, Herusugondo; Zainuri, Muhammad; Raharjo, Budi
JURNAL SAINS DAN MATEMATIKA Volume 20 Issue 4 Year 2012
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (4174.288 KB)

Abstract

Ketergantungan pestisida pada sentra produksi bawang merah Kabupaten Tegal telah menimbulkan pencemaran logam berat kadmium (Cd) di dalam tanah dan tanaman bawang merah. Kadar logam tersebut telah melebihi ambang batas yang dapat mengganggu kesehatan manusia. Tujuan penelitian ini adalah melakukan analisis kandungan kadmium pada akar, daun dan umbi tanaman bawang merah dari Tegal. Metode penelitian dilakukan melalui analisis kandungan logam berat kadmium dalam tanaman bawang merah  dibandingkan dengan kontrol menggunakan metode spektrofotometri atom absorbansi (AAS). Hasil penelitian memperlihatkan kandungan kadmium pada umbi, daun dan akar bawang merah di Tegal pada umur 20 hari berturut-turut adalah sebagai berikut 3,4805 mg/g; 4,1374 mg/g dan 7,9175 mg/g. Kandungan kadmium pada umbi, daun dan akar bawang merah umur 50 hari atau usia panen berturut-turut adalah sebagai berikut 1,8331 mg/g; 1, 8331 mg/g dan 3,5323 mg/g. Hasil penelitian yang diperoleh menunjukkan konsentrasi kadmium yang melampaui batas ambang aman bagi makanan, kesehatan dan lingkungan.    Pesticide dependence on onion production centers Tegal has caused heavy metal pollution of cadmium (Cd) in the soil and plant onions. This metal content exceeds the threshold that can impair human health. The purpose of this study was to analyze lead content in root, leaves and bulb of red onion from Tegal. Research methods to analyze the content of heavy metals in the soil compared to the control using atomic absorbance spectrophotometry (AAS). The research results showed lead content in roots, leaves and roots of onion at the age of 20 days in a row is as follows 3,4805 mg/g; 4,1374 mg/g and 7,9175 mg/g. The results obtained shows that cadmium concentrations exceed safe thresholds for food, health and the environment.
The Growth of Botryococcus braunii Microalgae as a Lipid Producer in a Mixed Medium of Coconut Water and Seawater Saputro, Bintoro Rudi; Kusdiyantini, Endang; Kusumaningrum, Hermin Pancasakti
JURNAL SAINS DAN MATEMATIKA Volume 23 Issue 4 Year 2015
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.477 KB)

Abstract

Energy needs in fuel sector tend to increase for supporting human life. Green microalgae (Chlorophyceae) can be used as an alternative solutions for bioenergy. Botryococcus braunii is lipid producer microalgae which can be processed further into biodiesel. Microalgae growth medium such as Walne can be substituted with coconut water that reducing economical cost. This research aims to determine the growth of B. braunii using coconut water and its effect on lipid production. The experiment conducted into five treatments of coconut water consist of P0 (0%), P1 (10%), P2 (7.5%), P3 (5%), and P4 (2.5%) for five days incubation and three repetitive step. Microalgae growth was calculated according to cell count using hemocytometer. The results showed that 2.5% coconut water substitution exhibited the best growth rate and lipid production, i.e. the amount of 547 cells / ml and produced lipid level according to these treatment increased 179% comparing with control.
Analisis Fusan Hasil Fusi Protoplas Intraspesies Pichia manshurica DUCC-015 (Analysis of Fusant from Protoplast Fusion Intraspecies of Pichia manshurica DUCC-015) Roslenawati, Roslenawati; Kusumaningrum, Hermin Pancasakti; Pujiyanto, Sri
JURNAL SAINS DAN MATEMATIKA Volume 22 Issue 1 Year 2014
Publisher : JURNAL SAINS DAN MATEMATIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (6086.358 KB)

Abstract

The intraspecies protoplast fusion of P. manshurica DUCC-015 was conducted in searching the fusant with greater inulinase production. Inulinase on Dahlia variabilis Willd tuber from Baturraden-Purwokerto showed inulinase activity 0,683 IU/mL. Inulinase is an enzyme that catalyzes the hydrolysis reaction of inulin polysaccharides into fructose and or fructooligosacarides. The aims of this research was revealing fusant from intraspecies protoplast fusion process of P. manshurica DUCC-015 followed by analyzing of their inulinase productivity and activity. The research metodology protoplast fusion process consist of lysis of cell wall, protoplast fusion and regeneration of intraspecies fusant P. manshurica DUCC-015. Analysis of fusant were done by Schiff Basic Fuchsin staining of fusant nuclei, also measuring the inulinase activity and inulinase production comparing with their parent. The inulinase activity will be analyzed by T-Test Independent Two Sample on 95% Confidence interval of the difference use Statistical Product and Service Solution programme (SPSS). The result of experiment gaining fusant with regeneration capability, ploidi diversity  of fusant, inulinase activity about 0,965 IU/mL while their parent 0,622 IU/mL and inulinase production 0,736  IU/mL comparing with 0,731 IU/mL during 42 hours incubations. The fusant indicated the increase of inulinase activity and production  compared with their parent.   Keywords: Pichia manshurica DUCC-015, fusant, inulinase    Fusi protoplas intraspesies Pichia manshurica DUCC-015 telah dilakukan untuk mencari fusan dengan produksi inulinase yang lebih tinggi. Inulinase pada umbi tanaman Dahlia variabilis Willd dari Baturraden-Purwokerto memperlihatkan aktivitas sebesar 0,683 IU/mL. Inulinase merupakan enzim yang mengkatalisis reaksi hidrolisis polisakarida inulin menjadi fruktosa dan atau fruktooligosakarida. Penelitian ini bertujuan untuk mendapatkan fusan hasil fusi protoplas intraspesies P. manshurica DUCC-015 yang memiliki aktivitas inulinase lebih tinggi. Rancangan percobaan fusi protoplas terdiri dari isolasi protoplas, fusi protoplas dan regenerasi fusan. Analisis fusan menggunakan pewarnaan  Fuchsin pada inti sel, mengukur aktivitas dan produksi inulinase fusan. Aktivitas inulinase dianalisis dengan Uji T Test Dua Sampel Independen pada taraf kepercayaan 95% menggunakan program Statistical Product and Service Solution (SPSS). Hasil penelitian menunjukkan fusi protoplas intraspesies P. manshurica     DUCC-015 menghasilkan aktivitas inulinase mencapai 0,965 IU/mL dibandingkan induk sebesar 0,622 IU/mL dan produksi inulinase 0,736  IU/mL pada inkubasi selama 42 jam. Fusan  mengindikasikan kenaikan aktivitas dan roduksi inulinasi dibandingkan induk. Kata kunci : Pichia manshurica DUCC-015, fusan, inulinase.
Aktivitas Enzim dan Identifikasi Fenotipik Isolat Kapang Aspergillus Kelompok Flavi Dari DUCC (Diponegoro University Culture Collections) Karima, Radhita; Rukmi, MG Isworo; Kusumaningrum, Hermin Pancasakti
Bioma : Berkala Ilmiah Biologi Vol. 22, No 1, Tahun 2020
Publisher : Departemen Biologi, Fakultas Sains dan Matematika, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (438.824 KB) | DOI: 10.14710/bioma.22.1.1-7

Abstract

Fungi has an important role as decomposer since they can produce hydrolytic enzymes.   The enzyme produced by fungi can be useful for industrial and biotechnology purposes.  Aspergillus section Flavi are known to have the ability in producing several enzymes. The aims of this study were to find out the ability of  Aspergillus  section  Flavi  from  Diponegoro  University  Culture  Collection  in  producing  amylase, protease  and  cellulase  enzymes  and  also  identify  the  species  using  phenotypic  method.  The  fungal enzymatic activity was examined by calculating enzymatic index on specific media. The results showed that all isolates have a potential as enzyme producers.  Phenotypic identification were characterized based on  macro- and micromorphology.  Isolates F130, F80CB, F138 dan F10A were identified as A.tamarii, while F43 and F20A were identified as A.flavus.
Co-Authors Agung Suprihadi Agung Suprihadi Agus Sabdono Agus Setiadi Agus Subagio Anggoro, Naufal Sebastian Annisa Fadillah Annisa Nur Ayuningtyas Anto Budiharjo Arina Tri Lunggani Aris Ismanto Asih Rismiarti, Asih Athried Elsima Azis Rifai Baskoro Rochaddi Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Chatarina Umbul Wahyuni Choirunnisaa, Nadia Maharani Jasmine Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Clara Yully Diana Ekaristi Denis Denny Nugroho Sugianto Dinda Khairunnisa, Dinda Doktorasaintifika, Heradita Kaniaazzahra Dudi Hardianto, Dudi Eiffeliena Nur'aini Fisikaningputri Purwienanti Eko Purnomo Elke Gildantia Elsima, Athried Endang Dwi Purbajanti Endang Kusdiyantini Erfianti, Tia Fiona Aqhila Dewi Fitri Arum Sasi, Fitri Arum Furgeva, Natasha Gentur Handoyo Hadi Endrawati Heksa Raihan Hererapratita Aysha Hapsari Herida, Azalia Puspa Hersugondo Hersugondo Hersugondo Hersugondo Herusugondo Herusugondo Imam Misbach Imam Mishbach Indah Sulistyarini Indras Marhaendrajaya Is Helianti IS HELIANTI Istiana, Rohma Ita Widowati Izzudin, Maulana Zidan Jihadi, Muhammad Shulhan Joedono Soedarsono Joedoro Sudarsono Karima, Radhita Kurniawati, Mufida Budi Lilis Sugiarti Lilis Sugiarti Lina Mulyawati Maria, Atina Mawarni, Shelina Nurunnisa Maya Aresteria MG Isworo Rukmi Monalita, Ramadhebi Muhammad Amal Nurhakim, Muhammad Amal Muhammad Anwar Djaelani Muhammad Iskandar Zulkarnain Muhammad Iskandar Zulkarnain Muhammad Mulyadi, Muhammad Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Nurhayati Nurhayati Okti Hajeng Kristiadi Rahayu, Annisa Rizky Rejeki Siti Ferniah Retno Hartati Risma Wiharyanti Rizko, Nurmalisa Robertus Triaji Mahendrajaya Roslenawati Roslenawati Rudhi Pribadi Safirah, Dearesty Sarwo Edi Wibowo, Sarwo Edi Siti Nur Jannah Siti Nur Jannah Solly Aryza Sonny Abdi Setiyawan, Sonny Abdi Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Rustini Subagiyo Subagiyo Sulis Setyowati, Sulis Suryani, Oda Gracia Ariela Sutrisno Anggoro Tony Hadibarata, Tony Tri Rahayu, Hesti Triwibowo Yuwono Wahyu Dewi Utari Haryanti Wahyuningsih, Candra Widianingsih Widianingsih Wijanarka Wijanarka Yann Hardivillier Yason Lukman Sudjito, Yason Lukman Yosef Purwoko Yoshua Mario Sumbodo Yudi Yunanto Yumna Rahmadias Hanifa Yunior William Susanto Yuvita Muliastuti