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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Jurnal Akademika Biologi International Journal of Marine and Aquatic Resource Conservation and Co-existence Prosiding KPSDA Jurnal Kelautan Tropis E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology Scripta Biologica Cendekia Journal of Pharmacy Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Hermin Pancasakti Kusumaningrum

Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University

Author-ID : 303161

Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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DIGITALISASI PENGELOLAAN KEUANGAN UMKM KABUPATEN BATANG Clara Yully Diana Ekaristi; Hermin Pancasakti Kusumaningrum; Denis; Maya Aresteria; Eiffeliena Nur'aini Fisikaningputri Purwienanti
Jurnal Abdimas Ilmiah Citra Bakti Vol 4 No 3 (2023)
Publisher : STKIP Citra Bakti

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i3.2253

UMKM atau usaha mikro, kecil, dan menengah merupakan bagian yang sangat penting dan strategis dalam peningkatan perekonomian nasional, terutama berperan dalam mendorong pertumbuhan ekonomi negara, apalagi saat ini pemerintah sedang berupaya memulihkan keadaan ekonomi pasca Covid-19. UMKM yang tangguh diawali dengan pemahaman pengelolaan keuangan yang baik. Kecamatan Bandar merupakan salah satu kecamatan di Kabupaten Batang yang memiliki banyak potensi sumberdaya yang dapat dikembangkan. UMKM di kecamatan Bandar kabupaten Batang Jawa Tengah yang terdiri dari berbagai jenis usaha yang semakin berkembang. Permasalahan yang dialami UMKM tersebut adalah masalah dan kendala dalam hal pengelolaan keuangan, hal ini disebabkan dengan adanya keterbatasan pemahaman pengelolaan keuangan baik secara sederhana maupun digital. Berlatar belakang dari permasalah ini, kegiatan digitalisasi pengelolaan keuangan UMKM Kabupaten Batang sebagai mitra dalam program ini. Program ini menggunakan metode ceramah dan diskusi. Kegiatan ini diawali dengan memberikan pre tes dan postes. Dilihat dari hasil penyebaran kuesioner peserta yang berprofesi sebagai wirausaha, dan memiliki berbagai jenis usaha. Dilihat dari sebelum sosialisasi digitalisasi pengelolaan keuangan terhadap peserta pelaku UMKM memiliki tingkat pemahaman sebesar 4-18% dan sesudah sosialisasi kegiatan mencapai tingkat pemahaman sebesar 100% meskipun masih tahap awal penggunaan digital. Dengah dilakukannya kegiatan sosialisasi ini, dapat dilihat bahwa tingkat pemahaman dan pengetahuan pengelolaan keuangan secara digital bagi pelaku UMKM unbu kecamatan Bandar mengalami peningkatan sehingga lebih meningkatkan kualitas dan produktivitas UMKM tersebut sehingga dapat meningkatkan perekonomian masyarakat pedesaan.

Isolation and Morphological Characterization of Lead Tolerant Bacteria Associated with Perna viridis Yoshua Mario Sumbodo; Fiona Aqhila Dewi; Okti Hajeng Kristiadi; Annisa Nur Ayuningtyas; Heksa Raihan; Muhammad Zainuri; Hermin Pancasakti Kusumaningrum
Buletin Oseanografi Marina Vol 12, No 3 (2023): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v12i3.59350

Lead is a heavy metal contamination that is released into sea waters and cannot be decomposed so it accumulation and magnification along the food chain. Efforts to explore bacteria that have tolerance and have the potential to become lead reduction agents are a strategic step in remediating lead contamination. One mechanism for lead bioremediation by bioaccumulation is to utilize metallothionein protein which can potentially be obtained from isolates of green mussel associated bacteria. Bacterial isolates associated with green mussels were obtained by isolating the bacteria and testing the tolerance of the bacterial isolates using Luria Bertani media which was added with Pb(NO3)2 at a concentration of 100 ppm and its multiples to determine their tolerance to lead. The bacterial isolates obtained were characterized based on colony and cell morphology. The results of the lead resistance test carried out showed that 11 bacterial isolates were obtained and 3 bacterial isolates had tolerance to lead with a lead concentration of 400 ppm. Bacterial isolates that are tolerant to lead show changes in cell morphology to reduce the impact of exposure to lead which has a lethal effect, because the resulting decrease in cell surface area can reduce lead absorption.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.38048/jailcb.v4i3.2253

Profiling of Seawater Bacterial Diversity in Tanjung Mas Port Using 16S rRNA eDNA Metabarcoding and Next-Generation Sequencing (NGS) Anggoro, Naufal Sebastian; Kusumaningrum, Hermin Pancasakti; Budiharjo, Anto; Wijanarka, Wijanarka; Zainuri, Muhammad; Hadibarata, Tony
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 1 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.1.61-70

Tanjung Emas Port is the entry and exit point for trade commodities, both regional and international filled with many ships. This condition makes biofouling a very massive process in that place by various types of marine bacteria. The initial formation of a biofilm is relevant to bacterial diversity, colonization and adhesion. The objective of the study was assessing bacterial diversity in relation to with biofouling within Tanjung Mas Port Semarang, by using 16S rRNA eDNA metabarcoding Next-Generation Sequencing (NGS). Seawater samples from aquatic sites of Tanjung Mas harbor was used for DNA extraction and amplification of the 16S rRNA V-3-V4 hypervariable region, followed by sequencing and library construction of eDNA Metabarcoding. Sequence processing and analysis was performed in QIIME 2 and RStudio using DADA2 for advanced sequencing processing and Phyloseq. The results of this research showed that bacteria is the predominant taxon constituting 100% of the community. The taxon consists of Proteobacteria (49.38%), Bacteroidota (8.67%), and Firmicutes (8.88%). Alphaproteobacteria (20.92%) and Gammaproteobacteria (12.39%) dominate at the Class level, emphasizing their versatility and ecological influence. At the Order and Family levels reveals the prevalence of Rhodobacterales (10.04%), Chitinophagales (2.53%), Rhizobiales (3.61%), Rhodobacteraceae (5.67%), Saprospiraceae (3.63%), and Rhizobiaceae (2.15%). It was found that the unculturable taxa dominance in Tanjung Mas Port was 44.66%. These taxonomic entities contribute significantly to the taxonomic and functional diversity of the microbial community, influencing nutrient cycling, organic matter degradation, ecosystem stability and biofilm formation.

Isolasi DNA Daun Jeruk Bali Merah (Citrus maxima Merr.) dengan Modifikasi Metode Doyle and Doyle Rizko, Nurmalisa; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti; Pujiyanto, Sri; Erfianti, Tia; Mawarni, Shelina Nurunnisa; Tri Rahayu, Hesti; Khairunnisa, Dinda
Berkala Bioteknologi Vol. 3 No. 2 November 2020
Publisher : Berkala Bioteknologi

Show Abstract | Download Original | Original Source | Check in Google Scholar

ABSTRAK Jeruk bali merah (Citrus maximaMerr) merupakan tanaman lokal yang tersebar di wilayah Indonesia. Keunggulan tanaman tersebut adalah nutrisi yang tinggi yang terdiri dari vitamin C996,773 mg/L, riboflavin, natrium (Na), kalsium (Ca), serta senyawa metabolit lainnya yang merupakan sumber antioksidan yang baik. Dalam masa pandemi ini, kebutuhan vitamikhusunya dalam menghadapi Pandemi Covid19.Pengembangan potensivarietas unggul dari jeruk bali perlu dilakukan salah satunya dengan analisis genetik. Tahap awal analisis genetik suatu tanaman adalah isolasi DNA tanaman tersebut. Penelitian ini bertujuan untuk mengisolasi DNA pada daun jeruk bali dengan metode Doyle & Doyle yang dimodifikasi dengan penambahan CTAB untuk mengatasi kadungan polisakarida dan senyawa polifenol yang tinggi dalam tanaman jeruk bali. Sampel DNA jeruk bali yang telah diisolasi di analisis menggunakan NanoDrop dengan absorbansi (A260/A280). Hasil pada penelitian ini, sampel DNA yang dianalisis menggunakan NanoDrop memiliki konsentrasi 220,8ng/µlserta kemurnian sebesar 1,93.Kata kunci : Jeruk bali (Citrus maxima), isolasi DNA, CTAB

Molecular Docking Studies of Marine Sulfated Polysaccharides: Exploring Green Seaweed’s Role Against SARS-CoV-2 Spike Glycoprotein Herida, Azalia Puspa; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Sabdono, Agus; Subagiyo, Subagiyo; Wahyuningsih, Candra
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 4 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.4.567-575

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is a virus responsible for the infectious disease of COVID-19 (Coronavirus Disease 2019), whose development is still being monitored. One way to deal with the virus’s development is by searching for natural-based medicines that prevent and treat SARS-CoV-2 infection. The abundant biodiversity can be used as a source of treatment. Among many natural resources, seaweed is one of the natural resources rich in bioactive components. Sulfated polysaccharide is one of the potential bioactive compounds in seaweed because it has antiviral effects and the potential to treat SARS-CoV-2. This research aims at increasing the potential of Karimunjawa green seaweed sulfate polysaccharides for antiviral capabilities in SARS-CoV-2 through molecular docking. This research began with identifying the morphology of Karimunjawa seaweed. Identify the components of sulfated polysaccharide compounds based on literature studies according to the species that have been identified. Analysis of the antiviral ability of SARS-CoV-2 based on its binding ability to the SARS-CoV-2 target protein through a molecular docking computational program and testing drug compounds using the Lipinski rule. Based on the research results, it is known that the seaweed obtained from Karimunjawa based on morphology, belongs to the genus Kappaphycus. Molecular docking with a ligand and spike glycoprotein (6LZG) resulted in the hexadecanoic acid compound having a binding free energy of -5.3 kcal.mol-1, which was the compound with the lowest yield compared to other test compounds. The prediction of the physicochemical properties of all test compounds fulfills Lipinski's five rules and has the potential to be used as medicinal compounds.

Geospatial Modeling of the Nitrate Distribution as an Indicator of Aquatic Fertility in the Lagoon Waters of the Mangrove Information Center (PIM), Pekalongan Zainuri, Muhammad; Suryani, Oda Gracia Ariela; Ismanto, Aris; Handoyo, Gentur; Rifai, Azis; Rochaddi, Baskoro; Endrawati, Hadi; Kusumaningrum, Hermin Pancasakti; Jihadi, Muhammad Shulhan; Hadibarata, Tony
Jurnal Kelautan Tropis Vol 27, No 3 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i3.24081

In the lagoon area of the Mangrove Information Center (PIM), Pekalongan, annual flooding leads to inundation in some land areas, particularly pond areas, lagoons, and residential areas. The area of submergence due to tidal floods in 2020 reached 783.99 hectares. The construction of embankments to the south of the lagoon as protection against flooding has transformed the northern area into a lagoon. Our research, crucial for understanding and managing these environmental changes, aims to determine and analyze the content and distribution of nitrate in the Lagoon Area of Mangrove Information Center Waters, Pekalongan. This was achieved through a thorough process of in situ observations based on purposive sampling at six sampling sites on June 22, 2023. The nitrate distribution data was then interpolated with a 2D current hydrodynamic model, ensuring the most accurate representation of the data. The value of nitrate content in the Mangrove Information Center lagoon ranges between 0.0065-0.1072 mg/L. This distribution pattern, influenced by deposits of mangrove litter, the depth of the lagoon, and water circulation, reflects the accumulation of physical and chemical influences in the waters.

MOLECULAR CHARACTERIZATION, PHYTOCHEMICALS SCREENING, AND MOLECULAR DOCKING OF CARDAMOM (Wurfbainia compacta), AND SAMBILOTO (Andrographis paniculata) AGAINST COVID-19 Kurniawati, Mufida Budi; Budiharjo, Anto; Kusumaningrum, Hermin Pancasakti; Ferniah, Rejeki Siti
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 11 No. 2 (2024)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2024.3005

Cardamom and Sambiloto are phytopharmaceutical plants that produce phytochemical compounds that have the potential to be used to increase immunity against COVID-19. because they contain carotenoids, phenols, anthocyanins, saponins, alkaloids and steroids. This research aims to obtain the molecular characteristics of Cardamom and Sambiloto plants from the Gunungpati area, Semarang by ITS primer, testing phenolic phytochemicals, tannins, flavonoids, saponins and alkaloids followed by molecular docking tests with the 6WX4 protein. SARS-CoV-2. Molecular characterization results show that Cardamom and Sambiloto are similar to Wurfbainia compacta MF802556.1 (100%) and Andrographis paniculate LC646073.1 (84.47%). The results of the phytochemical test screening showed that both plants contain flavonoids. Molecular docking tests were carried out with the compounds Quercetin, Avicularin, Naringenin, 5-hydroxy-7,8,2',5' tetramethoxyflavone, and Retinoic Acid. Retinoic Acid as a test ligand has the greatest potential in inhibiting the 6WX4 protein in the SARS-CoV-2 virus with a binding affinity value of -7.28 and RMSD 0.00.

Molecular Characterization of Nannochloropsis sp. Based on tufA Genetic Marker and Potential Test of Nannochloropsis sp. as a Cadmium (Cd) Heavy Metal Bioremediation Agent Setyowati, Sulis; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Suprihadi, Agung; Doktorasaintifika, Heradita Kaniaazzahra; Herida, Azalia Puspa; Wahyuningsih, Candra; Jannah, Siti Nur; Wijanarka, Wijanarka
Buletin Oseanografi Marina Vol 14, No 1 (2025): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v14i1.66422

Cadmium (Cd) heavy metal pollution not only affects aquatic ecosystems but also has toxic effects on human health. Bioremediation using microalgae Nannochloropsis sp. is considered more economical and sustainable for overcoming heavy metal pollution. This study aims to molecular characterization of Nannochloropsis sp. with tufA gene markers, determine the effect of different Cd concentrations on the growth and morphology of Nannochloropsis sp. and the ability of Nannochloropsis sp. to absorb Cd concentrations. The methods used include DNA isolation, quantitative and qualitative DNA analysis, amplification of tufA gene, sequencing result analysis, phylogenetic tree analysis, and bioremediation test. The results of molecular characterization showed that Nannochloropsis sp. has similarities with N. oceanica strain BR2 plastid (CP044614.1) with 60% bootstrap value. Nannochloropsis sp. grew at different Cd concentration (0, 2, 4, and 6 ppm) but optimal at 0 ppm Cd concentration. Morphology of Nannochloropsis sp. showed morphological differences in the cell structure. The cell was broken and the color turned increasingly clear. Different concentrations of Cd metal on Nannochloropsis sp. growth significantly affected the decline in Nannochloropsis sp. growth. Nannochloropsis sp. has the potential to absorb heavy metal Cd with an efficiency that increases as the concentration reaching 62.6% at 6 ppm. It can be concluded that molecular characterization using the tufA marker was effective in showing that Nannochloropsis sp. had closed relation with N. oceanica strain BR2 plastids. Cadmium (Cd) exposure significantly reduced growth and caused cell damage, while Nannochloropsis sp. effectively adsorbed Cd and lowering Cd levels by 3.76 ppm.

Molecular Characterization of Microalgae Thalassiosira sp. Based on Genetic Marker tufA and Potential Test for Bioremediation of Heavy Metal Lead (Pb) Izzudin, Maulana Zidan; Kusumaningrum, Hermin Pancasakti; Zainuri, Muhammad; Nurhayati, Nurhayati; Jannah, Siti Nur; Wahyuningsih, Candra; Herida, Azalia Puspa; Doktorasaintifika, Heradita Kaniaazzahra
Jurnal Kelautan Tropis Vol 28, No 1 (2025): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v28i1.25463

Heavy metal lead (Pb) pollution in aquatic environments poses a serious threat to aquatic ecosystems and human health. The microalga Thalassiosira sp. has potential as a bioremediation agent for heavy metals; however, further studies are needed to maximize its capability. This study aims to molecularly characterize Thalassiosira sp. using the tufA gene, evaluate the effects of Pb on growth and cell morphology, and assess its Pb uptake efficiency. Methods included DNA isolation, phylogenetic analysis, growth measurement using a hemocytometer, Pb uptake testing via ICP-OES, and cell morphology analysis. Results showed that the tufA gene could molecularly characterize Thalassiosira sp., with a phylogenetic similarity of 99.53% to Conticriba weissflogii (MH571875.1). Pb significantly inhibited growth at concentrations of 0.03 ppm and 0.05 ppm and caused cell aggregation and pigment degradation at higher concentrations (2 ppm). This microalga exhibited the highest Pb uptake efficiency at lower concentrations (0.03–0.05 ppm), reaching 100%, while at 2 ppm, the efficiency decreased to 40%. This study highlights the potential of Thalassiosira sp. in remediating Pb, providing a foundation for the development of microalgae-based biotechnological applications.

Co-Authors Agung Suprihadi Agung Suprihadi Agus Sabdono Agus Setiadi Agus Subagio Alfiatuzzahra, Diva Anggoro, Naufal Sebastian Annisa Fadillah Annisa Nur Ayuningtyas Anto Budiharjo Ardan, Kanaya Chandra Ajeng Sotya Arina Tri Lunggani Aris Ismanto Asih Rismiarti, Asih Athried Elsima Azis Rifai Badzlin, Zahrah Syahmina Baskoro Rochaddi Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Chatarina Umbul Wahyuni Choirunnisaa, Nadia Maharani Jasmine Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Clara Yully Diana Ekaristi Denis Denny Nugroho Sugianto Denny Nurkertamanda Dinda Khairunnisa, Dinda Djuara P Lubis Doktorasaintifika, Heradita Kaniaazzahra Dudi Hardianto, Dudi Eiffeliena Nur'aini Fisikaningputri Purwienanti Eko Purnomo Elke Gildantia Elsima, Athried Endang Dwi Purbajanti Endang Kusdiyantini Erfianti, Tia Fiona Aqhila Dewi Fitri Arum Sasi, Fitri Arum Furgeva, Natasha Gentur Handoyo Hadi Endrawati Hapsari, Hererapratita Aysha Heksa Raihan Hererapratita Aysha Hapsari Herida, Azalia Puspa Hersugondo Hersugondo Herusugondo Herusugondo Hidayati, Bintang Nurul Imam Misbach Imam Mishbach Indah Sulistyarini Indras Marhaendrajaya Is Helianti IS HELIANTI Isnaeni, Aissya Safriliana Istiana, Rohma Ita Widowati Izzudin, Maulana Zidan Jihadi, Muhammad Shulhan Joedono Soedarsono Joedoro Sudarsono Karima, Radhita Kurniantoro, Vangga Deswanto Prasetyo Kurniawati, Mufida Budi Lilis Sugiarti Lilis Sugiarti Lina Mulyawati Maharani, Prabaswara Nasywa Maria, Atina Maulidya, Shaula Dzikra Mawarni, Shelina Nurunnisa Maya Aresteria MG Isworo Rukmi Monalita, Ramadhebi Muhammad Amal Nurhakim, Muhammad Amal Muhammad Anwar Djaelani Muhammad Iskandar Zulkarnain Muhammad Iskandar Zulkarnain Muhammad Mulyadi, Muhammad Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Nurhayati Nurhayati Okti Hajeng Kristiadi Pasha, Muhammad Affan Rahayu, Annisa Rizky Razana, Aqila Khaira Rejeki Siti Ferniah Retno Hartati Risma Wiharyanti Rizko, Nurmalisa Robertus Triaji Mahendrajaya Roslenawati Roslenawati Rudhi Pribadi Safirah, Dearesty Sarwo Edi Wibowo, Sarwo Edi Shasabila, Analies Devi Siti Nur Jannah Siti Nur Jannah Solly Aryza Sonny Abdi Setiyawan, Sonny Abdi Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Rustini Subagiyo Subagiyo Sulis Setyowati, Sulis Suryani, Oda Gracia Ariela Sutrisno Anggoro Tony Hadibarata, Tony Tri Rahayu, Hesti Triwibowo Yuwono Wahyu Dewi Utari Haryanti Wahyuningsih, Candra Widianingsih Widianingsih Wijanarka Wijanarka Yann Hardivillier Yason Lukman Sudjito, Yason Lukman Yosef Purwoko Yoshua Mario Sumbodo Yudi Yunanto Yumna Rahmadias Hanifa Yunior William Susanto Yuvita Muliastuti

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