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All Journal ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Bioma : Berkala Ilmiah Biologi SAINS DAN MATEMATIKA Jurnal Ilmu Lingkungan Jurnal Akademika Biologi International Journal of Marine and Aquatic Resource Conservation and Co-existence Prosiding KPSDA Jurnal Kelautan Tropis E-Dimas: Jurnal Pengabdian kepada Masyarakat Jurnal Bioteknologi & Biosains Indonesia (JBBI) Biosaintifika: Journal of Biology & Biology Education Journal of Applied Food Technology Berkala Bioteknologi NICHE Journal of Tropical Biology Scripta Biologica Cendekia Journal of Pharmacy Jurnal Pasopati : Pengabdian Masyarakat dan Inovasi Pengembangan Teknologi Jurnal Abdimas Ilmiah Citra Bakti (JAICB) Prosiding Konferensi Nasional PKM-CSR Makara Journal of Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Hermin Pancasakti Kusumaningrum

Biotechnology Study Program , Biology Department, Faculty Of Sains And Natural Sciences, Diponegoro University

Author-ID : 303161

Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Mathematics Medicine & Pharmacology Nursing Physics Public Health Social Sciences Other

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Aplikasi Konsorsium Tanaman Herbal untuk Mengatasi Jerawat Akibat Autoimun Suatu Upaya Pengembangan Traditional Biomedicine Yosef Purwoko; Hermin Pancasakti Kusumaningrum; Lilis Sugiarti; Hererapratita Aysha Hapsari
Cendekia Journal of Pharmacy Vol 4, No 1 (2020): Cendekia Journal of Pharmacy
Publisher : STIKES Cendekia Utama Kudus

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31596/cjp.v4i1.83

Jerawat merupakan salah satu penyakit kulit autoinflamasi yang terkait dengan autoimmun yang biasa dijumpai para remaja di Indonesia. Permasalahannya, ternyata penyakit ini mempunyai prevalensi seumur hidup sebesar 85% dan menimbulkan dampak mekanisme inflamasi kompleks yang melibatkan imunitas bawaan. Bahkan bagi wanita yang berusia di atas usia 25 tahun, pengobatan jerawat menggunakan obat kimiawi mempunyai tingkat kegagalan yang tinggi. Kebutuhan akan pengembangan obat tradisional menjadi salah satu solusi untuk mengatasi permasalahan tersebut, khususnya terkait dengan keamanan dan kemudahan dalam penggunaannya serta mempunyai efek samping yang lebih kecil. Tanaman herbal di Indonesia telah menarik banyak perhatian karena penggunaannya secara tradisional dalam kehidupan sehari-hari yang telah banyak memecahkan permasalahan terkait dengan beberapa penyakit kulit yang disebabkan oleh autoimun. Tujuan penelitian adalah menentukan aktivitas konsorsium tanaman herbal dalam menghambat mikroorganisma penyebab jerawat. Metode penelitian dilakukan dengan mengisolasi mikroorganisme penyebab jerawat dan melakukan uji penghambatan pertumbuhan mikroorganisme yang berasal dari pasien penderita jerawat dibandingkan dengan kontrol menggunakan rebusan konsorsium tanaman herbal dan menganalisis potensi aktivitas obat jerawat dari setiap tanaman menggunakan analisis PASS. Hasil penelitian memperlihatkan 21 jenis tanaman herbal mempunyai aktivitas dalam mengatasi penyakit kulit yang terkait dengan autoimun dan mikroorganisme. Uji aktivitas antimikroorganisme menunjukkan diameter zona hambatan pertumbuhan sebesar 2 - 5 mm sesuai dengan semakin banyaknya larutan. Hasil analisis PASS memperlihatkan bahwa tanaman sambiloto (Andrographis paniculata) memperlihatkan potensi tertinggi dalam mengobati jerawat dengan kemampuan sebesar 86%. Meskipun demikian, hasil penelitian memperlihatkan potensi tanaman herbal sebagai obat jerawat akibat autoimun akan mencapai hasil yang lebih optimal bila digunakan bersama-sama.

Antibacterial Activity of Microalgae Dunaliella salina, Tetraselmis chuii and Isochrysis galbana Against Aquatic Pathogens Ita Widowati; Muhammad Zainuri; Hermin Pancasakti Kusumaningrum; Yann Hardivillier
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 26, No 4 (2021): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.26.4.265-270

Recently, expanded consideration has been paid to the business and potentiality of microalgae. Some microalgae are at present being studied for their capacity to find important metabolites for the drug industry or aquacultural applications. Concerning these biotechnological challenges, there is a consistent exertion accommodated in both finding and taking advantage of new microalgal assets and fostering their putative business results or modern valorizations. The crude methanol extracts of three microalgae, Dunaliella salina, Tetraselmis chuii, and Isochrysis galbana, have been investigated for antibacterial activity using the disk diffusion method against aquatic pathogens of fish, shrimp, and shellfish e.g. Pseudomonas fluorescence and Vibrio harveyi. This research aimed to analyze the antibacterial activity of crude extract of these microalgae against aquatic pathogens. The disk diffusion method was used to investigate the antibacterial activity. The result showed that only T. chuii has the inhibition zone for both tested bacteria P. fluorescence and V. harveyi with the inhibition zone of 3.0 ± 0.6 mm on 100 µg. g–1 of extract concentration and 4.20 ± 1.1 mm against P. fluorescence on 10000 µg. g–1 of extract concentration. While D. salina and I. galbana have inhibition zone only for V. harveyi with the inhibition zone of 4.4 ± 0.6 mm and 3.2 ± 0.7 mm on 10000 µg. g–1 of extract concentration. These three microalgae may have potential use in aquatic pathogens as antimicrobial agents. It would be possible to develop biologically active compounds of microalgae as a functional feed for aquaculture.

Formation of Eco-friendly Silver Nanoparticle Microalgae using Chlorella vulgaris Hermin Pancasakti Kusumaningrum; Muhammad Zainuri; Widianingsih Widianingsih; Wahyu Dewi Utari Haryanti; Indras Marhaendrajaya; Robertus Triaji Mahendrajaya
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 24, No 1 (2019): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Biosynthesized silver nanoparticles (AgNPs) using organism have spurred great interest as a antimicrobial and biomedical agents. Green microalgae have advantages as they are easily available, grow rapidly and producing varieties metabolites. Synthesized of AgNPs from microalgae C. vulgaris offer environmentally antimicrobial agent. The objectives of the study is producing AgNPs microalgae using C. vulgaris as eco-friendly antimicrobial agent. The research methods was conducted by synthesizing silver nanoparticle microalgae using C. vulgaris following by characterization under UV–visible spectroscopy, transmission electron microscopy (TEM), and scanning electron microscope (SEM) and Energy-dispersive X-ray spectroscopy (EDX). The research result showed AgNPs C. vulgaris microalgae were produced with and without agitation treatment under different condition. The synthesized AgNPs C. vulgaris exhibited a maximum absorption at 312 nm and 398 nm, and EDX analysis had determined that abundance chemical elements presented in a sample were carbon and silver. The TEM analysis revealed that they are spherical form. The spot of EDX analysis showed the presence of silver atoms. The SEM analysis shows the spherical shaped with some silver particle inside of the cell. These resut indicated that formation of silver nanoparticle microalgae using C. vulgaris has been succesfully obtained under the treatment.

Application of Aquaculture Natural Food Produce by Protoplast Fusion Process of Dunaliella salina and Phaffia rhodozyma Hersugondo Hersugondo; Hermin Pancasakti Kusumaningrum; Muhammad Zainuri
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 15, No 4 (2010): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Recently, fishponder income tend to be static so that required an effort to accurately and efficiently one of them through aquacultures food diversification. So far, efforts to observe the pattern of introduction of a new feed, applications and their impact on aquaculture and fish farmers income has never been measured. Diversification of feed use protoplast fusion process from D. salina and P. rhodozyma which rich in natural carotenoids is needed in aquaculture because they will increase survival and body weight of animal farming. Different types of carotenoids can be combined through a process of protoplasm fusion making it cheaper, faster and efficient for diversification and development of carotenoid-rich diet. The specific objectives of this research is the development of natural food aquaculture with high carotenoid content using protoplasm fusion from the microalgae D. salina and yeast P. rhodozyma. The early stage in this research will cultivate recombinant followed by quantitative analysis of carotenoid production compared with controls. The second stage is the food diversification with different concentrations of recombinant and comparisons with commercial food followed by cost-benefit analysis. The research results have been obtained which most carotenoid-rich natural food resulted from recombinant fusion of D. salina and P. rhodozyma. Its application in vitro have increased body weight of shrimp comparing to artificial feed. In addition, recombinant food showing stable growth in both freshwater and salt water, can breed naturally and are safe for animal aquaculture consumption and also the environment. Key words : carotenoid, protoplast fusion, D. salina, P. rhodozyma, recombinant Selama ini pendapatan petani tambak cenderung statis sehingga diperlukan suatu upaya secara tepat dan efisien salah satunya melalui diversifikasi pakan. Sejauh ini usaha untuk mengamati pola introduksi suatu pakan baru, aplikasi dan dampaknya terhadap budidaya dan pendapatan petani tambak belum pernah terukur. Diversifikasi pakan mengggunakan pakan kaya karotenoid alami sangat dibutuhkan dalam budidaya perikanan karena terbukti dapat meningkatkan keloloshidupan dan menambah bobot hewan budidaya. Kedua jenis karotenoid β-karoten dan astaxantin dapat digabungkan melalui proses fusi protoplasma sehingga lebih murah, cepat dan efisien untuk diversifikasi dan pengembangan pakan kaya karotenoid. Tujuan khusus penelitian ini adalah pengembangan usaha budidaya untuk meningkatkan pendapatan petani tambak melalui diversifikasi pakan akuakultur dengan kandungan karotenoid tinggi hasil fusi protoplasma alga Dunaliella salina dan khamir Phaffia rhodozyma. Tahap awal dalam penelitian ini akan melakukan kultivasi pakan rekombinan Dunaliella salina dan Phaffia rhodozyma diikuti analisis produksi karotenoid secara kuantitatif dibandingkan dengan kontrol. Tahap kedua adalah diversifikasi pakan dengan perbedaan konsentrasi fusan dan perbandingan dengan jenis pakan lain diikuti dengan analisis untung rugi. Kata kunci : karotenoid, fusi protoplas, D. salina, P. rhodozyma, rekombinan

Relationship Between Stage of Gonad Maturity and Level of Osmotic Work of Sea Cucumber, Paracaudina australis Widianingsih Widianingsih; Muhammad Zainuri; Sutrisno Anggoro; Hermin Pancasakti Kusumaningrum; Retno Hartati
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 23, No 1 (2018): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

The holothuroid spawning can be succesful depend on varoius factors such as salinity, temperature, primary productivity and other physiological adaptation toward environment influences. The salinity fluctuation is one of the environment factors, which influence and can cause various osmotic pressure of coelomic fluid of Holothoroid and also gonad maturity stage. Holothuroid (sea cucumber), Paracaudina australis, is overexploited in Kenjeran waters, Surabaya, East Java. The study was aimed to reveal relationship between gonad maturity stage and level of osmotic work of sea cucumber P. australis. Fifty samples of P. australis were collected monthly. Coelomic fluid samples of approximately 200–1000 µl were obtained from all samples using disposable insulin syringes. There were 37 holothuroids classified in the late maturity (stage 5), 74 holothuroid in the earlier maturity stage (stage 1). Gonad maturity stage 5 (late maturity) could be reached on December 2016. Contrasly, the gonad maturity stage 1 (earlier maturity) was found on August 2016. The analysis of coelomic fluid showed that the average value of level of osmotic work ranged 70 to 91 mOsm/L H2O in the earlier gonad maturity stage. Contrasly, in the late maturity, the average value of level of osmotic work ranged 118 to 156 mOsm/L H2O. There is relationship between gonad maturation stage and level of osmotic work. From stage of gonad maturity 1 to 5, there were upward trend level of osmotic work in coelomic fluid of P. australis.

Aplikasi Pakan Alami Kaya Karotenoid untuk Post Larvae Penaeus monodon Fab. (Applications Rich Natural Carotenoids Feed for Post Larvae of Penaeus monodon Fab.) Hermin Pancasakti Kusumaningrum; Muhammad Zainuri
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 18, No 3 (2013): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Udang windu (Penaeus monodon Fab.) merupakan komoditas unggulan Indonesia sedangkan ketersediaan benih udang windu mengalami penurunan mencapai 50% disebabkan serangan berbagai penyakit dan keterbatasan pakan. Aplikasi pakan rekombinan hasil fusi protoplas yang kaya karotenoid telah terbukti meningkatkan bobot dan kelulushidupan larva udang windu skala laboratorium. Teknik fusi protoplas telah terbukti mampu menghasilkan rekombinan yang memiliki gabungan karotenoid dari berbagai alga sehingga jumlah karotenoidnya semakin meningkat. Dunaliella menghasilkan karotenoid β-karoten dan zeaxantin dalam jumlah besar sedangkan Chlorella menghasilkan lutein, β-karoten dan astaxanthin. Tujuan khusus penelitian ini adalah mengaplikasikan pakan alami hasil fusi protoplas kedua jenis mikroalga pada larva udang windu di Balai Budidaya Air Payau Jepara untuk meningkatkan ketahanan terhadap serangan penyakit. Penelitian ini melakukan kultivasi induk dan rekombinan diikuti analisis pigmen karotenoid dan aplikasi pakan rekombinan secara in vitro. Aplikasinya pada skala in vitro telah meningkatkan berat badan udang dan kelulushidupannya dibandingkan pakan buatan dan pakan alami saja. Selain itu pertumbuhan pakan rekombinan stabil baik di air tawar maupun air asin, mampu berkembangbiak secara alami dan konsumsinya aman bagi hewan budidaya dan lingkungan. Kata kunci: karotenoid, fusi protoplas , Dunaliella salina, Chlorella vulgaris, Tiger shrimp (Penaeus monodon Fab.) is one of the main commodities of aquaculture products in Indonesia. The availability of Tiger shrimp larvae has decreased up to 50% due to the attack of various diseases and feed limitation. Application of feed from recombinant of protoplast fusion rich in carotenoids have been shown to increase weight and survival rate of tiger shrimp larvae on laboratory scale. Protoplast fusion technique has been shown to produce a recombinant that has combined various carotenoids from algae thus increasing their catotenoids number. Dunaliella produce carotenoids β - carotene and zeaxantin in large numbers while Chlorella produce lutein, β - carotene and astaxanthin. The specific objective of this research is to apply natural food of protoplast fusion recombinant from both of microalgae to Tiger shrimp larvae in Brackish Water Aquaculture Center Jepara to improve resistance to disease. This study conducted cultivation of parent and recombinant followed by carotenoid pigment analysis and application of recombinant feed in vitro. Application on a in vitro scale have increased body weight and shrimp survival compared to artificial food and natural food. Additionally feed recombinant growth were stable in both freshwater and saltwater. They also able to breed naturally and safe for animal consumption and environmental. Keywords: carotenoid, protoplast fusion, Dunaliella salina, Chlorella vulgaris

Identifikasi Spesies Mikroalga dari BBPBAP Jepara secara Morfologi dan Molekuler menggunakan 18S rDNA Elke Gildantia; Rejeki Siti Ferniah; Anto Budiharjo; Agung Suprihadi; Muhammad Zainuri; Hermin Pancasakti Kusumaningrum
Buletin Oseanografi Marina Vol 11, No 2 (2022): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v11i2.39703

Mikroalga merupakan organisme eukariot bersel satu yang habitatnya berada di perairan. Suatu spesies mikroalga koleksi kultur BBPBAP Jepara berpotensi menghasilkan astaxantin dalam jumlah tinggi. Namun, spesies ini belum di karakterisasi secara molekuler. Penelitian ini bertujuan untuk memperoleh karakter morfologi dan molekuler pada isolat mikroalga dari BBPBAP Jepara menggunakan 18S rDNA guna memastikan spesies untuk pengembangan potensinya. Tahapan penelitian yang dilakukan meliputi kultivasi mikroalga, pengamatan pola pertumbuhan, pengamatan morfologi, isolasi DNA, amplifikasi menggunakan marka 18S rDNA, elektroforesis, analisis data sekuens, dan filogenetik menggunakan aplikasi NJ Plot dan MEGA 7. Hasil karakterisasi secara morfologi sel isolat mikroalga BBPBAP Jepara memperlihatkan bentuk bulat, berwarna hijau dengan ukuran 4,5 μm, tidak memiliki flagela, dan motilitas yang pasif. Hasil karakterisasi molekuler menggunakan 18S rDNA dari isolat mikroalga BBPBAP memperlihatkan kemiripan tertinggi dengan Chlorella sorokiniana sebesar 99%. Microalgae are single-celled eukariyotic organisms whose habitat is in the waters. A species of microalgae from the Jepara BBPBAP culture collection was potential to produce high amounts of astaxantin. However, this species has not been detected molecularly previously. This study aimed to obtain the morphological and molecular characters of microalgae isolates from BBPBAP Jepara using 18S rDNA to ascertain the species and its potential development. The stages of the research carried out include; microalgae cultivation, growth pattern observation, morphological observation, DNA isolation, amplification using 18S rDNA markers, electrophoresis, sequence data analysis, and phylogenetic using NJ Plot and MEGA applications 7. Results of morphological characterization of the Jepara BBPBAP showed that cell of microalgae isolates had a round shape, green color with a size of 4.5μm, has no flagella and passive motility. The results of the molecular characterization using 18S rDNA showed that isolate BBPBAP had highest similarity with Chlorella sorokiniana about 99%.

Analisis Nitrat dan Fosfat Terhadap Sebaran Fitoplankton Sebagai Bioindikator Kesuburan Perairan Muara Sungai Bodri Imam Mishbach; Muhammad Zainuri; Widianingsih Widianingsih; Hermin Pancasakti Kusumaningrum; Denny Nugroho Sugianto; Rudhi Pribadi
Buletin Oseanografi Marina Vol 10, No 1 (2021): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v10i1.34645

Nitrat dan fosfat adalah unsur nutrien yang menjadi pembatas kelimpahan fitoplankton. Kandungan nitrat dan fosfat di perairan muara akan dipengaruhi oleh faktor biologi, fisika dan kimia perairan, yang menyebabkan timbulnya suatu persebaran. Hal tersebut menjadi permasalahan di Muara Sungai Bodri, Kendal. Tujuan dari penelitian ini adalah untuk mengkaji kandungan nitrat dan fosfat beserta pola persebarannya, serta keterkaitannya dengan struktur populasi fitoplankton sebagai bioindikator kesuburan perairan di Perairan Muara Sungai Bodri, Kabupaten Kendal. Penelitian dilaksanakan berdasarkan metoda observasi dengan pendekatan spatio-temporal. Sejumlah 9 stasiun penelitian dan 3 kali waktu sampling yaitu 30 Juni, 1 September dan 27 Oktober 2018. Kandungan nitrat dan fosfat serta kelimpahan fitoplankton ditetapkan sebagai variabel terikat. Sedangkan parameter fisika dan kimia perairan seperti suhu, salinitas, derajat keasaman (pH), oksigen terlarut, kecerahan dan arus ditetapkan sebagai variabel kontrol. Hasil penelitian menunjukan bahwa kandungan nitrat berkisar 0,49-0,9 mg/l serta kandungan fosfat berkisar 0,01-0,29 mg/l yang menunjukkan bahwa perairan Muara Sungai Bodri, Kabupaten Kendal dalam kondisi subur/eutrofik. Kelimpahan fitoplankton menunjukkan nilai kisaran diantara 2.356 sampai dengan 162.626 sel/l, yang tersusun oleh 5 kelas dan 45 jenis/species dengan pola persebaran berbentuk konvergen yang bertumpu pada stasiun 3 dan 5. Perairan Muara Sungai Bodri, Kabupaten Kendal menunjukkan tingkat kesuburan yang tinggi atau eutrofik. Nitrate and Phosphate are the nutrient element which influence as limiting factor to the phytoplankton population. Nitrate and Phosphate in the water were influence by physical and chemical factors, and will affect to the distribution. The distribution of nitrate and phosphate will affect the distribution of phytoplankton population. The purpose of the research is to analyze the distribution of phytoplankton population due to the influence of nitrate and phosphate contents at Bodri Estuary, Kendal. The research was apllied based on the spatio-temporel approach. There are 9 stations and triple sampling of 30 June, 1st Septembre and 27 Octobre, 2018. The nitrate and phosphate contents and phytoplankton abundance was set as dependent variables. The parameters temperature, salinity, pH, Dissolved Oxygen, transparency and current were used as variable control. The research result show the nitrate content range between 0,49-0,9 mgr./l and the phosphate content range between 0,01-0,29 mgr./l, which indicate that the Bodri Estuary, Kendal is eutrophic. The distribution of nitrate and phosphate contens show a model of convergen on west area of sampling and tend to along the coastline in the east part. The phytoplankton abundance show a range of 2.356 - 162.626 cell /l, which composed of 5 Class and 45 species.

Effect of Silver Nano Particle Microalgae Chlorella pyrenoidosa and Dunaliella salina on Growth and Survival of Penaeus monodon Larvae Hermin Pancasakti Kusumaningrum; Muhammad Zainuri; Indras Marhaendrajaya; Agus Subagio; Widianingsih Widianingsih; Hadi Endrawati; Annisa Fadillah; Muhammad Iskandar Zulkarnain; Yuvita Muliastuti; Imam Misbach
Buletin Oseanografi Marina Vol 10, No 2 (2021): Buletin Oseanografi Marina
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/buloma.v10i2.35483

Penaeus monodon is one of the most important farmed crustaceans. Its also known as Asia Tiger Shrimp because its carapace and abdomen are transversely banded with red and white. The use of synthetic antibiotic in aquaculture had caused problems related to health and environmental safety. Chlorella pyrenoidosa and Dunaliella salina are photosynthetic microalgae. Silver nano particle in microalgae C. pyrenoidosa and D. salina had synthesized and showed their growth stability. They offer a potency to be exploited to supported growth and survival of shrimp larvae. The objective of the study was the application of silver nano particle in microalgae C. pyrenoidosa and D. salina on P. monodon larvae. The research methodology was carried out by making microalgae C. pyrenoidosa and D. salina containing silver nano particle and used as feed of shrimp larvae. Observations were made on the growth and survival of shrimp larvae compared to both microalgae and common feed. The results showed that the P. monodon larvae have the higher growth and survival rate with microalgae C. pyrenoidosa at the beginning of their growth compared to D. salina. However, microalgae without nanosilver and common feed showed a better result for growth and activity of shrimp larvae.

Optimization and Stability of Total Pigments Production of Fusan from Protoplast Fusion of Microalgae Dunaliella and Chlorella in vivo: Attempts on Production of Sustainable Aquaculture Natural Food Hermin Pancasakti Kusumaningrum; Muhammad Zainuri
International Journal of Marine and Aquatic Resource Conservation and Co-existence 2014: IJMARCC Volume 1 Issue 1 Year 2014
Publisher : International Journal of Marine and Aquatic Resource Conservation and Co-existence

Diversification of feed rich in natural carotenoids is needed in aquaculture because they will increase survival and body weight of animal farming. Different types of important carotenoids such as β-carotene, zeaxanthine can be combined through a process of protoplast fusion making it cheaper and faster. Crustaceans can not synthetize carotenoid de novo and they need it to provide nutrition and possibly disease resistance, pigmentation and esthetic value. Green microalgae produce carotenoids and can be manipulated easily by protoplast fusion. The research was conducted to obtain some fusants from interspesies protoplast fusion of D. salina and C. vulgaris. Interspecies protoplas fusion was carried out by protoplast isolation, protoplast fusion and protoplast regeneration. Microscopic and cell analysis will used to confirm positive regenerate protoplast. Analysis of the obtained fusants is limited to morphological description due to the complexity and variability of fusant. The stabilities of fusants obtained were examined by successive subcultures. The result revealed that the fusant from D. salina and C. vulgaris maintain their stability in growth and increased carotenoid production during four periods of subculturing. This result suggesting the potency of fusant to be used as food supplement. Key words: Carotenoid, Protoplast fusion, Dunaliella, Chlorella

Co-Authors Agung Suprihadi Agung Suprihadi Agus Sabdono Agus Setiadi Agus Subagio Anggoro, Naufal Sebastian Annisa Fadillah Annisa Nur Ayuningtyas Anto Budiharjo Arina Tri Lunggani Aris Ismanto Asih Rismiarti, Asih Athried Elsima Azis Rifai Baskoro Rochaddi Bintoro Rudi Saputro, Bintoro Rudi Budi Raharjo Chatarina Umbul Wahyuni Choirunnisaa, Nadia Maharani Jasmine Christina Ratna Handayani, Christina Ratna Christina Retna Handayani, Christina Retna Clara Yully Diana Ekaristi Denis Denny Nugroho Sugianto Dinda Khairunnisa, Dinda Doktorasaintifika, Heradita Kaniaazzahra Dudi Hardianto, Dudi Eiffeliena Nur'aini Fisikaningputri Purwienanti Eko Purnomo Elke Gildantia Elsima, Athried Endang Dwi Purbajanti Endang Kusdiyantini Erfianti, Tia Fiona Aqhila Dewi Fitri Arum Sasi, Fitri Arum Furgeva, Natasha Gentur Handoyo Hadi Endrawati Heksa Raihan Hererapratita Aysha Hapsari Herida, Azalia Puspa Hersugondo Hersugondo Hersugondo Hersugondo Herusugondo Herusugondo Imam Misbach Imam Mishbach Indah Sulistyarini Indras Marhaendrajaya Is Helianti IS HELIANTI Istiana, Rohma Ita Widowati Izzudin, Maulana Zidan Jihadi, Muhammad Shulhan Joedono Soedarsono Joedoro Sudarsono Karima, Radhita Kurniawati, Mufida Budi Lilis Sugiarti Lilis Sugiarti Lina Mulyawati Maria, Atina Mawarni, Shelina Nurunnisa Maya Aresteria MG Isworo Rukmi Monalita, Ramadhebi Muhammad Amal Nurhakim, Muhammad Amal Muhammad Anwar Djaelani Muhammad Iskandar Zulkarnain Muhammad Iskandar Zulkarnain Muhammad Mulyadi, Muhammad Muhammad Zainuri Mulyawati, Lina Natasha Furgeva Nurhayati Nurhayati Nurhayati Nurhayati Okti Hajeng Kristiadi Rahayu, Annisa Rizky Rejeki Siti Ferniah Retno Hartati Risma Wiharyanti Rizko, Nurmalisa Robertus Triaji Mahendrajaya Roslenawati Roslenawati Rudhi Pribadi Safirah, Dearesty Sarwo Edi Wibowo, Sarwo Edi Siti Nur Jannah Siti Nur Jannah Solly Aryza Sonny Abdi Setiyawan, Sonny Abdi Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Pujiyanto Sri Rustini Subagiyo Subagiyo Sulis Setyowati, Sulis Suryani, Oda Gracia Ariela Sutrisno Anggoro Tony Hadibarata, Tony Tri Rahayu, Hesti Triwibowo Yuwono Wahyu Dewi Utari Haryanti Wahyuningsih, Candra Widianingsih Widianingsih Wijanarka Wijanarka Yann Hardivillier Yason Lukman Sudjito, Yason Lukman Yosef Purwoko Yoshua Mario Sumbodo Yudi Yunanto Yumna Rahmadias Hanifa Yunior William Susanto Yuvita Muliastuti

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