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Journal : International Journal Of Science, Technology

Characteristics of Curds With Milk Clotting Enzyme from Indonesian Local Isolate of Lactic Acid Bacteria Putranto, Wendry; Mustopa, Apon; Mamangkey, Jendri; Aritonang, Netty
International Journal of Science, Technology & Management Vol. 1 No. 2 (2020): July 2020
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v1i2.18

Abstract

To get the potential of lalcat acid bacteria isolate to produce Milk Clotting Enzyme (MCE), it is necessary to screen milk clotting activity both quantitatively and qualitatively. Through qualitative observation, the characteristics of the curd resulting from enzyme activity can be obtained. MCE is a protease that has the characteristics of milking. Based on the results of this observational research, the curd characteristic produced can be used as a benchmark to determine the length of time of fermentation and optimization of the determination of ammonium sulfate precipitation concentration. Isolate BAL shows the results of a compact curd at a fermentation time of 25 hours at 37 ? and the optimization results of the deposition of ammonium sulfate which shows the characteristics of a compact curd by 45% ammonium sulfate.
Bioprospecting For Bacterial Endophytes Associated With Zingiberaceae Family Rhizomes In Sibolangit Forest, North Sumatera mamangkey, Jendri; Lucas William Mendes; Mada Triandala Sibero; Pilidai Endorina Nadapdap
International Journal of Science, Technology & Management Vol. 1 No. 1 (2020): May 2020
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v1i1.7

Abstract

The present study was conducted aiming to isolate and characterize endophytic bacterial isolates with antibacterial ability, phosphate solubilization, and proteolytic activity from rhizomes of the Zingiberaceae family (Etlingera sp., Globba patens, Globba pendula, and Zingiber multibracteata). Nineteen bacterial isolates were obtained from Zingiberaceae rhizomes with isolate codes of EZS27, EZS18, EZS19, EZS25, EZS16, EZS08, EZS09, EZS13, EZS20, EZS14, EZS10, EZS11, EZS03, EZS05, EZS06, EZS43, EZS45, EZS47, and EZS28. The screening of the endophytes for antibacterial activity was done through the paper disc method. Four bacterial isolates presented antibacterial activities. EZS06 isolate inhibited the growth of EPEC (11 mm), P. vulgaris ATCC 13315 (10 mm), and L. monocytogenes BTCC B693 (9 mm). Also, EZS20 isolate inhibited the growth of S. aureus ATCC 29213 (17 mm), EZS28 isolate inhibited MRSA ATCC 43300 (8.6 mm), and EZS45 isolate inhibited S. Epidermidis ATCC 12228 (9 mm). The EZS19, EZS03, and EZS16 isolates dissolved the phosphate most effectively. Eight isolates (EZS19, EZS47, EZS27, EZS25, EZS09, EZS20, EZS45, and EZS06) showed the best protease activity. In general, our results showed that the endophytic bacterial strains can be used as a new and useful antibacterial agent since it showed antibacterial activity and chemical diversity. Furthermore, it also has the potential for exploitation in a wide variety of medical, agricultural, and industrial areas.
Endophytic Bacteria and Fungi from Indonesian Medicinal Plants with Antibacterial, Pathogenic Antifungal and Extracellular Enzymes Activities: A Review Jendri Mamangkey; Lucas William Mendes; Arman Harahap; Dana Briggs; Cengiz Kayacilar
International Journal of Science, Technology & Management Vol. 3 No. 1 (2022): January 2022
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v3i1.428

Abstract

The habitat of endophytic bacteria in plant tissues making it automatically used by plants to protect themselves against pathogenic bacteria. Endophytic bacteria have been applied in various fields, including agriculture, pharmacy, medicine, and biotechnology. This review was carried out to summarize recent studies focusing on the diversity of endophytic bacteria derived from traditional medicinal plants which have antibacterial activity and extracellular enzymes. This article was prepared and written by referring to literature studies collected from books and online journal publication. The literature review of endophytic bacteria focused on Vernonia anthelmintic, Saurauia scaberrinae, and Ki rinyuh (Chromolaena odorata) plants. The results revealed that endophytic bacteria obtained from Vernonia anthelmintic plant is from Micrococcus, Bacillus, Pseudomonas, Stenotrophomonas, and Pantoea genus which possess the same biological properties as the host plant, producing antibacterial activity. Saurauia scaberrinae plant successfully isolated endophyte fungus Phoma sp. which produces phomodione (4) and cercosporamide. Phomodione (4) exhibit inhibitory activity against S. aureus, P. ultimum, S. sclerotiorum and R. solani. Cercosporamide exhibits inhibitory activity against S. aureus. Endophytic bacteria were also isolated from Ki rinyuh (Chromolaena odorata), including BECB3, BECB 4, BECA 8, BECA 5, BECA 1, and BECA 10 isolates which have extracellular enzyme activities such as ?-amylase, ?-amylase, cellulase, chitinase, and protease. Understanding endophytic bacteria in medicinal plants can help researchers apply them effectively.
Purification and Characterization of Rennin Like Protease from Enterococcus faecium 1.15 Isolated from Indonesia Fermented Bakasam Putranto, Wendry; Suryaningsih, Lilis; Zaenal, Apon; Mamangkey, Jendri
International Journal of Science, Technology & Management Vol. 4 No. 4 (2023): July 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i4.900

Abstract

The protease enzyme produced by Lactic Acid Bacteria (LAB) with characteristics similar to rennin is called Rennin Like Protease (RLP). The purpose of the study was to obtain information on the biochemical characteristics of RLP produced by Enterococcus faecium 1.15 as a basis for the utilization of the enzyme. The first stage of the study was observation of the growth curves and the potential of the isolates to produce RLP, biochemical characterization. Rennin Like Protease (RLP) produced by Enterococcus faecium 1.15 which has been purified using Sephadex G-50 has a molecular weight of 42 kDa with biochemical characters, with an optimum temperature of 40 ? and pH of 4.5. The comparison between MCA/PA with a value of 3.74 which is close to the MCA value of Calf Rennet is 1.
Production Of Antimicrobial And Bioactive Peptides From Bakasam Using Enterococcus Faecium 1.15 As A Starter Setiyadi Putranto, Wendry; Gumilar, Jajang; Wulandari, Eka; Pratama, Andry; Mamangkey, Jendri
International Journal of Science, Technology & Management Vol. 4 No. 6 (2023): November 2023
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v4i6.982

Abstract

The Development Of Traditional Fermented Food Products Into Functional Food, Utilizing Indigenous Microorganisms, Represents A Novel Innovation. Lactic Acid Bacteria (Lab), Which Are Abundantly And Readily Isolated From Native Fermented Foods, Hold Promise For The Exploration Of Local Lab Strains – A Strategic Step Towards The Development Of ProbioticBased Foods In Indonesia. Enterococcus Faecium 1.15 Isolated From Bakasam Beef, Has Demonstrated The Capability To Produce Extracellular Proteases (4.83 U/Mg) And Displayed Inhibitory Effects Against Pathogenic Bacteria. The Production Of Bioactive Peptides, With A Size Less Than 30 Kda, Could Be Achieved Through Bakasam Fermentation Using E. Faecium 1.15 As A Starter For A Period Of 7 Days. This Study Promotes An Opportunity To Harness Local Microorganisms, Particularly Lab Strains In The Utilization Of Bioactive Peptides As Well As Enhancing The Nutritional Value Of Traditional Fermented Foods.
Mapping the Knowledge Landscape of Local and Traditional Ecological Knowledge (LEK/TEK) in Mangrove Ecosystems: A Bibliometric Analysis in the Past Decade Sunarto, Sunarto; Mamangkey, Jendri
International Journal of Science, Technology & Management Vol. 6 No. 5 (2025): September 2025
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v6i5.1360

Abstract

Local Ecological Knowledge (LEK) and Traditional Ecological Knowledge (TEK) play a crucial role in mangrove ecosystem management, restoration, and community livelihoods. Over the past decade, scholarly interest in this topic has grown, yet research remains scattered across regions and disciplines. This study employed a bibliometric analysis of Scopus-indexed publications from 2014–2024, focusing on LEK/TEK in mangrove ecosystems. A total of 93 open-access articles and conference papers were identified. Results show two major research clusters: (i) ecosystem restoration, conservation, and ecosystem services, and (ii) ethnobotany and medicinal applications of mangrove species. Global publication patterns highlight Indonesia, India, and Bangladesh as leading contributors, while citation impact is distributed across both ecological and ethnobotanical studies. Despite growing attention, gaps remain in cross-regional comparisons, integration of LEK/TEK into policy, and interdisciplinary approaches bridging ecological, cultural, and governance perspectives. The findings provide a comprehensive overview of global scholarship and highlight future directions to strengthen the role of LEK/TEK in sustaining mangrove ecosystems.
Trends and Knowledge Gaps In Fish-Based Fermented Foods: A Bibliometric Analysis From The Last Decade (2015-2024) Mamangkey, Jendri; Grovy Naibaho, Frans; Rosenta Purba, Ika; Yusriya Hibatullah, Alnadia; Aryanti Pada Soa, Christina
International Journal of Science, Technology & Management Vol. 6 No. 6 (2025): November 2025
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v6i6.1369

Abstract

Fish-based fermented foods are integral to traditional diets across Asia, Africa, and Europe, valued for both their nutritional role and cultural significance. Increasingly, they are also studied for potential functional properties such as probiotic activity and bioactive compounds, yet their global research landscape has not been systematically assessed. This study presents a bibliometric analysis of global research on fish-based fermented foods published between 2015 and 2024. Data were retrieved from the Scopus database and analyzed using the Bibliometrix R package and Biblioshiny interface. A total of 263 documents were identified across 145 sources, authored by 1,150 researchers. The field has shown rapid growth, with an annual publication increase of 27.01% and an average of 28.48 citations per article, indicating strong scholarly visibility. Most contributions originated from East and Southeast Asia, particularly China, South Korea, and Thailand, reflecting the cultural and culinary importance of fermented fish in these regions. Author productivity followed Lotka’s law, with a small group of core researchers driving sustained output. Keyword co-occurrence and thematic mapping revealed dominant clusters around fermented fish sauce, lactic acid bacteria, and microbial diversity, alongside emerging themes related to fatty acids and bacterial communities. Despite these advances, the analysis highlighted notable gaps, including limited international collaboration and underrepresentation of research from Africa and parts of South Asia. These findings suggest that while the field is expanding and diversifying, greater cross-regional engagement and methodological innovation are needed to fully explore the nutritional, functional, and socio-economic dimensions of fish-based fermented foods.
Bio-Priming Using Indigenous Pseudomonas Fluorescens To Improve Local Upland Rice (Padi Gogo) Production Of Paebiu Kolopua And Pae Wuna Cultivars Asri Razad; Gusti Ayu Kade Sutariati; Tresjia C Rakian; Jendri Mamangkey; Marina Silalahi
International Journal of Science, Technology & Management Vol. 2 No. 6 (2021): November 2021
Publisher : Publisher Cv. Inara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46729/ijstm.v2i5.337

Abstract

Research was performed to evaluate the effect of biopriming technique using indigenous rhizobacteria in improving local upland rice from Southeast Sulawesi. Randomized group design was applied in this research using factorial pattern consisting of 2 factors. The first factor was upland rice cultivars consisting of 2 (two) levels of Paebiu Kolopua (V1) and Pae Wuna (V2) cultivars. Meanwhile, the second factor was rhizobacteria isolates, consisting of 4 (four) levels, those are without rhizobacteria treatment (R0), KNS11 isolate (R1), KLKU02 isolate (R2) and KNW11 isolate (R3). Research results indicated that interaction between Paebiu Kolopua cultivar and KLKU02 isolate treatment significantly affected plants height with 29.22% improvement and the amount of rice per panicle with 176.33% improvement compared to the control. Therefore, KLKU02 isolate can be recommeded as the best treatment to improve the production and outcome of local upland rice plant.
Co-Authors Abdillah, Latifah Ningrum Adinugraha, Fajar Adisti Ratnapuri Adrian Hartanto, Adrian Ai Hertati, Ai Akmaliyah, Rizna Amani, Febriyanti Nur Andika, Vrista Andry Pratama, Andry Angela, Ezra Anggi Izdihar Mahaswari Apon Zaenal Mustopa Arfa Yanti, Nur Ario Betha Juanssilfero Aritonang, Netty Arman Harahap Aryanti Pada Soa, Christina Asri Razad Aulia Septiani Baehaqi Bethaniel Roy Matthew Cengiz Kayacilar Chairunnisa, Sheila Chihombori, Tatenda Calvin Dana Briggs Dian Winarty Budadana, Christyani DWI SURYANTO Ekawati, Nurlaili Elida Octavia Evi Yulinda Tpoenifu Fatimah Frans Grovy Naibaho Gulo, Joice Elma Miranda Gusniarty Simanjuntak Gusti Ayu Kade Sutariati Hibatullah, Alnadia Yusriya Huda Shalahudin Darusman Hutabarat, Anne Serafin I wayan Teguh Wibawan Irawan, Herman Ivan Julius Rajagukguk Jajang Gumilar Janed Lauren Soplantila Janed Lauren Soplantila Jefrino Reis Pereira Joice Elma Miranda Gulo Joko Pamungkas Joko Wahyono, Daniel Kartika Sari Dewi KIKI NURTJAHJA Lilis Suryaningsih Lomo, Jhoni Yakobus Lucas William Mendes Lukas Pardosi Mada Triandala Sibero Manguntungi, Baso Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Marina Silalahi Medyawanti Pane Mustopa, Apon Mutiara, Ilma Muttaqin, Salwa Zainum Napitupulu, Agnes Ester Napitupulu, Alexis Rachel Maria Rosauli Nelius Harefa Nitte, Tri Maharani Permata Nive Winda Sari Lumban Tungkup Novianti, Ela Nowlinda Kamuihkar Nur Amani, Febriyanti Nurfatwa, Maritsa Octavia, Elida Ode Adi Parman Rudia, La Ode Muhamad Iman Sulaiman, La Pereira, Jefrino Reis Pilidai Endorina Nadapdap Purba, Dian Pirma Natasari Putranto, Wendry Raden Mohamad Herdian Bhakti RATIH ASMANA NINGRUM Ratnapuri, Adisti Refli, Redoyan Ria Simanjuntak, Desy Riana, Febi Rifqiyah Nur Umami, Rifqiyah Nur Rika Indri Astuti Ripaldo Sihombing Riska Septia Wahyunigtyas Riska Septia Wahyuningtyas Rivaldi Sitinjak Rosdiana Virginia Rosenta Purba, Ika Sakka, Deni Frans Salwa Zainum Muttaqin Santhya Anaomi Tamansa Sari, Isti Kartika Sembiring, Geskia Avikasari Septiani, Aulia Serafin, Anne Setiyadi Putranto, Wendry Siahaan, Frisca Olivia Sitinjak, Rivaldi Situmorang, Agnes Feronika Sri Swasthikawati, Sri SUNARTO Sunarto Sunarto Sunarto Sunarto Talitha Amalia Tesalonika Febriani Zebua Tpoenifu, Evi Yulinda Tresjia C Rakian Tri Maharani Permata Nitte Trinugroho, Joko P Wafaretta, Erona Wahyuningtyas, Riska Septia Wijaya, Hans Yudi Purnomo Zaenal, Apon Zefanya Sipahelut