Garuda - Garba Rujukan Digital

CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.

Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.

ANTIOXIDANT ACTIVITY TEST AND ANALYSIS OF COMPOUNDS CONTAINED IN ANGELS’S TRUMPET FLOWER EXTRACT (Brugmansia suaveolens) Wuwungan, Axel; Manampiring, Nonny; Gedoan, Sukmarayu; Mokosuli, Yermia Semuel; Roring, Verawati Ida Yani
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12553

Indonesia is a highly biodiverse country, second only to Brazil. This gives Indonesia enormous potential in ethnopharmacology. Previous studies have shown that the leaves of the Brugmansia suaveolens plant, also known as Angel trumpet, have high antioxidant activity. The aim of this study is to test the antioxidant activity of B. suaveolens flowers and conduct quantitative screening to determine which compounds play the greatest role in their antioxidant activity. This research used the DPPH test and Gas Chromatography-Mass Spectrometry (GC-MS) screening. Ascorbic acid was used as the comparator in this study. Before the DPPH and GC-MS testing, the samples were extracted using the cold maceration technique with a 95% ethanol solvent. Then, the samples were concentrated using a rotary evaporator. The IC₅₀ value of the ethanol extract of B. suaveolens flowers is 196.16 µg/mL, while the IC₅₀ value of ascorbic acid, the comparator, is 3,814 µg/mL. This result shows that the ethanol extract of B. suaveolens flowers has moderate antioxidant activity. Screening bioactive compounds with GC-MS showed that Hexadecenoic acid and Octadecanoic acid play the biggest role in antioxidant activity. However, the compound with the highest content in the ethanol extract of B. suaveolens flowers is scopolamine.

The Antioxidant Activity of Red Fruit Extract (Pandanus conoideus L.) From Nabire Papua Migau, Natalina; Mokosuli, Yermia; Wurarah, Masje; Manampiring, Nonny
Indonesian Biodiversity Journal Vol. 5 No. 1 (2024): April 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i1.9174

Red fruit (Pandanus conoideus Lam) is a native Papuan plant which has been used by indigenous Papuan people as a medicinal plant. Papuan people believe that red fruit has a broad spectrum of pharmacological activity. This research aims to obtain antioxidant activity, namely reducing DPPH free radicals from Papuan red fruit ethanol extract. Samples of red fruit obtained from Nabire Papua. After determination, the red fruit is extracted using the maceration method. The ethanol extract of red fruit was then used for the DPPH antioxidant test method. The results showed that the ethanol extract of red fruit contains phenolic compounds: flavonoids, tannins; and steroids in high intensity. Ethanol extract of red fruit has very strong antioxidant activity (46.99 ppm; R2=0.99). Ethanol extract of Papuan red fruit has potential as a source of bioactive natural antioxidants.

ANALYSIS OF MANGROVE FOREST VEGETATION ON MOLAS BEACH, BUNAKEN DISTRICT, MANADO CITY Futwembun, Fransiska; Lawalata, Helen J; Setyawati, Iriani; Ogi, Nova L I M; Manampiring, Nonny; Mokosuli, Yermia
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10753

The lack of scientific information about the current condition of the mangrove ecosystem on the Molas beach in the Bunaken District of Manado City is an important reason to conduct a study related to the analysis of mangrove forest vegetation. The purpose of this research is to identify the types of mangroves and to find the Important Value Index (INP) of mangroves on the Molas beach in the Bunaken District of Manado City. Data collection was conducted using the line transect method with plots at only one observation station, which was then analyzed to determine the Importance Value Index (INP). Based on the observations, it was found that four types of mangroves were present at the research location. The dominant mangrove species are Bruguiera Cylindrica, Bruguiera gymnorrhiza, Aegiceras cosniculatum, and Ceriops tagal. The substrate condition, which is dominated by muddy sand, and the water quality parameters that affect mangrove growth generally still meet the standards for mangrove growth. The species Ceriops tagal has the highest values of Relative Density, Relative Frequency, and Relative Dominance, resulting in the highest INP. Meanwhile, the lowest INP was found in the species Bruguiera gymnorrhiza in the tree category

CHARACTERIZATION OF SAPROPHAGE INSECTS FORENSIC ENTOMOLOGY INDICATORS USING LOCAL PIGS Kandowangko, Sandy; Wurarah, Masje; Mokosuli, Yermia; Satiman, Utari; Gedoan, Sukmarayu
Indonesian Biodiversity Journal Vol. 5 No. 2 (2024): August 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i2.10755

Forensic entomology is the science of insects which is used to analyze cases related to forensics, namely death. This is based on the close relationship between humans and insects who live side by side. Insects can be a factor in determining the Postmortem Interval (PMI) so that it is useful in the forensic investigation process. Medicolegal insects are insects that are used as evidence in death cases. This research aims to obtain the diversity of insect species, the morphological characteristics of insects, the characteristics of insects in experimental pigs that died from hanging, poisoning and stabbing and based on the stage of decomposition. The results of this research examine temperature, soil pH, air humidity and the decomposition process of local pigs. The results of research on the decomposition stage in 3 deaths (hanging, stabbing, and poisoning) showed differences in the time of death, where the hanging carcass decomposed more quickly than the stabbed and poisoned carcass. In the decomposition stage, the first insect encountered is the order (Diptera) where these insects damage internal organs such as the brain and liver and also damage body cells. The temperature results of local pig carcasses placed outdoors ranged from 32.2-34.50C, while indoor carcass temperatures ranged from 26.77-33.43 0C. The degree of acidity (pH) of carcasses placed outdoors ranges from 5.13-8.53 while indoor carcasses ranges from 6.0-7.77. The environmental temperature of animal bodies placed outdoors ranges from 26.35-39,740C, while humidity ranges from 57.79-86.10%. The temperature of animal bodies in the room ranges from 27.54-31,520C, while humidity ranges from 71.50-85.37%.

Antibacterial Activity Of Sago Caterpillar Oil (Rhynchoporus bilineatus L.) From Minahasa Against Salmonella typhi and Staphylococcus aureus Pua, Devis Christian; Mokosuli, Yermia Semuel; Sumampouw, Harry Maurits
Indonesian Biodiversity Journal Vol. 5 No. 3 (2024): December 2024
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v5i3.10760

Sago caterpillars are ethnomedically used as food and medicine by the people of several tribes in Indonesia. Sago caterpillar oil contains active compounds, especially fatty acids that have antibacterial potential. Research has been carried out which aims to obtain the bioactive content of sago worm oil and the antibacterial activity of Salmonella typhi and Staphylococcus aureus bacteria. Sago caterpillars are obtained from the Minahasa region. Four to six instar sago caterpillars are used for oil isolation. Analysis of the content of sago worm oil using the Gas Chromatography Mass Spectrophotometry method. Meanwhile, the antibacterial test used the agar diffusion method using pure cultures of Salmonella thypii and Staphylococcus aureus. The results showed that the average sago caterpillar oil was 0.26 to 0.28 ml per head. The results of the antibacterial test showed the highest inhibition of S. aureus at a concentration of 1000 mg/L, namely 8.04 mm, while the inhibition of growth of S. thypii was highest at a concentration of 1000 mg/L, namely 7.69 mm. Sago caterpillar oil has antibacterial potential.

DEVELOPMENT OF ANTI MOSQUITO LOTION MADE FROM CLOVE (SYSYGIUM AROMATICUM), LANGUSEI (FICUS MINAHASSAE), AND APIS DORSATA BINGHAMI HONEYCOMB PLANTS Hermanus, Britney Putri; Naharia, Orbanus; Mokosuli, Yermia Semuel
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 1 (2024): Volume 7 No 1 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i1.24061

Mosquitoes are the main insect vectors that cause various tropical diseases, including malaria, filariasis, and various viral diseases, such as dengue fever. Indonesia is a country with a tropical climate whose environmental characteristics are very supportive of mosquito breeding. Mosquitoes are infectious vectors that must be controlled. Efforts to control mosquito vectors include using repellents. Repellent is a chemical that can keep insects away so that insect bites can be avoided. This research aims to determine the formula for anti-mosquito lotion made from cloves, langusei, and beehives and to determine the results of organoleptic, physical tests, and the strength of repellent anti-mosquito lotion made from cloves, langusei, and beehives. The method used in this type of research is the laboratory experimental method. The samples used in this research were clove leaves, langusei, and honeycomb. The analyses carried out were organoleptic tests, pH tests, homogeneity tests, and protection power tests. The lotion, with the addition of clove, langusei, and honey bee hive plant extracts, meets the organoleptic test, test requirements for physical quality and repellent lotion strength, namely having a smooth and soft lotion mass, the color and aroma of the lotion is typical of a mixture of clove, langusei, and honey bee hive plants. The results of the study showed that from variations in the concentration of cloveleaf extract, langusei, and honey bee nest (0%, 0.25%, 5%, 10%), the best results were formula IV, namely with a concentration of 10% which had the best physical properties, safe to use and the most effective protective power to repel mosquitoes.

DEVELOPMENT OF AN ANTI-MOSQUITO SPRAY GEL FORMULATION FOR A COMBINATION OF MARIGOLD FLOWERS (TAGETES ERECTA L.) AND CHRYSANTHEUM FLOWERS (CHRYSANTHEMUM INDICIUM L.) Mulder, Keysia Stephanie Tesalonica; Mokosuli, Yermia Semuel; Lawalata, Helen Joan
Jurnal Review Pendidikan dan Pengajaran Vol. 7 No. 1 (2024): Volume 7 No 1 Tahun 2024
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v7i1.24062

Mosquitoes are vectors of infectious diseases that must be controlled. One of the efforts to control mosquito vectors is by using anti-mosquito products, including anti-mosquito gel spray. Most anti-mosquito products are composed of chemicals that are harmful to health and the environment, so alternative anti-mosquito products made from natural ingredients are needed. The aim of this research is to determine an effective anti-mosquito gel spray formula from a combination of marigold flowers and chrysanthemum flowers. This research uses experimental methods to find the most effective gel spray formulation from different percentages of marigold and chrysanthemum flowers. The variations used are 0%, 2.5%, 5% and 10%. Tests were carried out using 30 adult female mosquitoes. The results obtained through the extraction of marigold flowers showed a yield of 8.922% and a yield of chrysanthemum flowers of 4.560%. The organoleptic test carried out on 10 panelists showed that on average the panelists thought they liked (in the range 4-5) their preference for color and aroma. , texture and reaction after 10 minutes of using the gel spray. The pH test showed that the four gel spray formulas had a pH of 6 or neutral. The results of the homogeneity test carried out with a glass object showed that the four gel spray formulas were homogeneous. The protective power of the 2.5% gel spray formula is 18.01%; gel spray formula 5% is 66.29% and gel spray formula 10% is 88.61%. From these results, it can be seen that a formula with a concentration of marigold and chrysanthemum flower extracts of 10% can be the most effective anti-mosquito gel spray. It is hoped that the results of this research will be useful for society in terms of using mosquito repellent that is environmentally friendly and safe for health.

Coconut Oil Processing as a Learning Medium and Implementation of P5 at SMA 1 Wori: Pengolahan Minyak Kelapa Sebagai Media Pembelajaran dan Implementasi P5 di SMA 1 Wori Mokosuli, Yermia; Mege, Revolson; Manampiring, Nonny; Matey, Meyer; Buyang, Dalle; Maidangkay, Vonny
Dinamisia : Jurnal Pengabdian Kepada Masyarakat Vol. 9 No. 2 (2025): Dinamisia: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Lancang Kuning

Show Abstract | Download Original | Original Source | Check in Google Scholar

North Sulawesi is identical with coconut plants, in addition to being a source of economy but also one of the leading commodities and inseparable from people's lives. However, until now coconut farmers still have difficulty getting quality and cheap consumption oil. Observing the problems of the community, State Senior High School 1 Wori raised it as material for the Pancasila Student Profile Strengthening Project (P5) Entrepreneurship Theme implementing coconut oil processing technology as well as a learning medium. However, the coconut oil processing carried out by students is still simple and of low quality. This activity aims to apply coconut oil processing technology as a learning medium and implementation of the Merdeka Belajar program at SMA 1 Wori to produce quality coconut oil. The method used is a combination of the Problem Based Learning method with the Life Skill Education approach to coconut processing entrepreneurship. Through this activity, students in addition to gaining knowledge and skills as well as direct experience of Life Skill Education to process coconut oil with technology and can produce quality coconut oil.

Title

Found 83 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 83 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 83 Documents
Search