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All Journal Jurnal Bios Logos JURNAL BIOMEDIK Jurnal Pendidikan Biologi Undiksha JSME MIPA UNIMA Jurnal Sains dan Teknologi Jurnal Pendidikan IPA Indonesia Abdimas Jurnal Bioedukatika Jurnal Biologi Tropis Dinamisia: Jurnal Pengabdian Kepada Masyarakat JURNAL PENDIDIKAN TAMBUSAI Molekul: Jurnal Ilmiah Kimia Jurnal Review Pendidikan dan Pengajaran (JRPP) Jurnal Pembelajaran dan Biologi Nukleus SOSCIED Jurnal Aplikasi dan Inovasi Iptek (JASINTEK) Jurnal Dedikasi Bioscientist : Jurnal Ilmiah Biologi Majalah Info Sains Seminar Nasional Pengabdian Kepada Masyarakat Indonesian Biodiversity Journal Innovative: Journal Of Social Science Research Molekul: Jurnal Ilmiah Kimia Indonesian Biodiversity Journal Servitium Smart Journal Jurnal Pendidikan Progresif Advances in Tropical Biodiversity and Environmental Sciences Metamorfosa: Journal of Biological Science Open Access DRIVERset
Mokosuli Yermia Samuel
Universitas Negeri Manado
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Medicine & Pharmacology Neuroscience Public Health Social Sciences Veterinary Other

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PENGEMBANGAN E-BOOK DAN PRAKTIKUM BERBASIS ANALISIS DNA LALAT BUAH ISOLAT LOKAL PADA MATERI GENETIKA DI SMA Djaila, Friscila Avianti; Semuel, Mokosuli Yermia; Wurarah, Masje
Jurnal Review Pendidikan dan Pengajaran Vol. 8 No. 1 (2025): Volume 8 No. 1 Tahun 2025
Publisher : LPPM Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/jrpp.v8i1.41423

Abstract

Penelitian ini bertujuan untuk mengembangkan dan menghasilkan media pembelajaran e-book dan praktikum berbasis analisis DNA lalat buah isolat lokal pada materi genetika di SMA yang layak digunakan. Penelitian ini merupakan penelitian pengembangan (Research and Development) dengan model ADDIE yang terdiri 5 tahapan, yaitu Analysis, Design, Development, Implementation, dan Evaluation. Instrumen tes pada penelitian ini adalah angket, wawancara dan dokumentasi. Lembar angket penilaian diberikan kepada subjek penelitian yaitu ahli materi, ahli media, guru mata pelajaran biologi dan siswa kelas XII di SMA Negeri 1 Pagimana. Hasil penelitian ahli materi dengan persentase 93% kriteria sangat valid, ahli media dengan persentase 96% kriteria sangat valid, guru biologi dengan persentase 97% kriteria sangat valid dan respons siswa dengan persentase 88% kriteria sangat baik. Dapat disimpulkan bahwa media e-book dan praktikum berbasis analisis DNA lalat buah isolat lokal pada materi genetika di SMA produk yang dihasilkan sangat layak untuk digunakan sebagai media pembelajaran.
Anticancer Activity Of Apis Dorsata Binghami Nest Extract From West Uluway, Tana Toraja District Patandean, Selvrining; Mokosuli, Yermia Semuel; Ogi, Nova LIM; Djarang, Rievo H; Manampiring, Nonny; Moko, Emma Mauren
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12750

Abstract

Cancer is the leading cause of death in the world, with a high prevalence in Indonesia, especially breast cancer. Conventional therapies such as chemotherapy and radiotherapy have various limitations, including severe side effects and cancer cell resistance. This study aims to explore the anticancer potential of the ethanol extract of Apis dorsata Binghami nests originating from Uluway Barat Village, Tana Toraja Regency. Extraction was carried out using the maceration method with 96% ethanol solvent, with a yield of 51.42%, and bioactive compounds were identified through GC-MS analysis. Anticancer activity tests were carried out in vitro on MCF-7 breast cancer cells using the resazurin assay method . The results showed that the extract contained various bioactive compounds such as flavonoids, furanones, and aromatic aldehydes which are known to have antioxidant and anticancer activities. The IC50 value obtained was 434.30 µg/mL, indicating weak cytotoxic activity. Nevertheless, this extract showed significant ability to kill cancer cells at certain concentrations, especially 15.63 µg/mL which reduced cell viability by 44.39%. These results indicate that A. dorsata Binghami nests contain compounds that have the potential as natural anticancer agents, but require further research for fractionation and identification of more specific active compounds and further testing in vivo
Anticancer Activity Of Apis Dorsata Binghami Nest Extract From West Uluway, Tana Toraja District Patandean, Selvrining; Mokosuli, Yermia Semuel; Ogi, Nova LIM; Djarang, Rievo H; Manampiring, Nonny; Moko, Emma Mauren
Indonesian Biodiversity Journal Vol. 6 No. 1 (2025): April 2025
Publisher : Universitas Negeri Manado

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.53682/ibj.v6i1.12750

Abstract

Cancer is the leading cause of death in the world, with a high prevalence in Indonesia, especially breast cancer. Conventional therapies such as chemotherapy and radiotherapy have various limitations, including severe side effects and cancer cell resistance. This study aims to explore the anticancer potential of the ethanol extract of Apis dorsata Binghami nests originating from Uluway Barat Village, Tana Toraja Regency. Extraction was carried out using the maceration method with 96% ethanol solvent, with a yield of 51.42%, and bioactive compounds were identified through GC-MS analysis. Anticancer activity tests were carried out in vitro on MCF-7 breast cancer cells using the resazurin assay method . The results showed that the extract contained various bioactive compounds such as flavonoids, furanones, and aromatic aldehydes which are known to have antioxidant and anticancer activities. The IC50 value obtained was 434.30 µg/mL, indicating weak cytotoxic activity. Nevertheless, this extract showed significant ability to kill cancer cells at certain concentrations, especially 15.63 µg/mL which reduced cell viability by 44.39%. These results indicate that A. dorsata Binghami nests contain compounds that have the potential as natural anticancer agents, but require further research for fractionation and identification of more specific active compounds and further testing in vivo
Insect Biodiversity in Mangrove Area of Tatengesan Village, Pusomaen District in Southeast Minahasa, North Sulawesi Feby Febrianty Toding Rante; Yermia Semuel Mokosuli; Iriani Setyawati; Ferny Margo Tumbel; Helen Joan Lawalata
Advances in Tropical Biodiversity and Environmental Sciences Vol. 7 No. 2 (2023): June 2023: Pages 40-86
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2023.v07.i02.p04

Abstract

The existence of insects cannot be separated from the condition of their habitat. The mangrove area of ​​Tatengesan Village is one of the ecotourism site in Minahasa, North Sulawesi, that can support the survival of insects. This study aimed to determine the distribution and diversity of insects and the factors that influence the insect diversity in the mangrove area. The type of research used is descriptive quantitative research by conducting data collection activities, analyzing data and interpreting data with the aim of making a description of the events that occurred. The animals were exploratory collected by using insect nets (sweep net), beating cloth (beating), and bare hands (hand collecting). The results showed that there were 1225 individual insects consisting of 14 insect species, which were divided into 14 genera, 11 families and 6 orders of insects. The insect diversity index value was 0.27 which was classified as low. The dominance index value was 0.90 which was classified as high. The evenness index value was 0.10 which was classified as low.
Identification of Bacteria Associated in the Small Intestine of White-Tailed Forest Rat (Maxomys hellwandii) in South Minahasa Regency, North Sulawesi Megawati Meylin Weken; Revolson Alexius Mege; Helen Joan Lawalata; Yermia Semuel Mokosuli; Iriani Setyawati
Advances in Tropical Biodiversity and Environmental Sciences Vol. 8 No. 2 (2024): June 2024: Pages 58-118
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2024.v08.i02.p01

Abstract

Sulawesi Island in the Wallaceae line area has diverse endemic animals, including the white-tailed forest rats. White-tailed forest rat (Maxomys hellwandii) is one of the endemic animals in North Sulawesi, so this study aimed to determine the type of bacterial genus in the small intestine of Maxomys hellwandii. This research used a laboratory-based qualitative descriptive method using a pour plate isolation method and bacterial identification based on morphological, and physiological characters with biochemical tests. The small intestines of Maxomys hellwandii taken from the South Minahasa forest area, North Sulawesi were used as samples of this research. The results of the study obtained 22 isolates of bacteria that were successfully isolated and have been identified based on morphological characteristics, and physiology with biochemical tests based on the book Bergey's Manual of Systematic Bacteriology suspected 14 isolates as genus Bacillus and 8 isolates as genus Lactobacillus.
Isolation of Salmonella sp. from Eggs Commonly Sold in Tondano Market, Minahasa, North Sulawesi Britney Meissy Pandoh; Herry Maurits Sumampouw; Helen Joan Lawalata; Danny Christian Posumah; Anita Constanci Christine Tengker; Yermia Semuel Mokosuli
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 1 (2025): February 2025: Pages 1-66
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i01.p01

Abstract

Various types of eggs are one of the foodstuffs that are in great demand by the community, especially the people of North Sulawesi, because they are cheap and easy to obtain. Furthermore, eggs have the nutritional content of animal protein, which is good for humans. Numerous studies on eggs have revealed that microorganisms, including Salmonella sp., can contaminate them. This study aims to detect Salmonella sp. bacteria in various types of eggs in the Tondano Market, Minahasa, North Sulawesi. This research uses a descriptive method (qualitative method) to describe an event objectively. We collected up to three samples, the first of which is located outside the market. Three samples of purebred chicken eggs, duck eggs, and quail eggs were taken. Then, the second point inside the market was taken: 3 samples of purebred chicken eggs, duck eggs, and quail eggs from the same trader. To prepare the samples, the eggshells were separated from the insides, then 2 grams of the eggshells were crushed and mixed with 5 ml of Buffered Peptone Water (BPW) 0.1%. This mixture was then added to 10 ml of Rappaport Vassiliadis (RV) media, followed by scraping onto Salmonella Shigella Agar (SSA) media, and then inoculating onto Xylose Lysine Deoxycholate agar (XLD) media. Four eggshell samples tested positive for Salmonella sp. bacteria: duck eggs (TB 1), quail eggs (TP 1), broiler eggs (TA 2), and quail eggs (TP 2), while two samples were negative: broiler eggs (TA 1) and duck eggs (TB 2). In contrast, two samples were negative for Salmonella sp., namely broiler eggs (TA 1) and duck eggs (TB 2).
The Use of Beauveria bassiana as Biological Control against the Pepper Aphid Insect Pest Myzus persicae Sulz Semuel Harpandi; Jantje Ngangi; Helen Joan Lawalata; Sukmarayu Pieter Gedoan; Utari Satiman; Yermia Semuel Mokosuli
Advances in Tropical Biodiversity and Environmental Sciences Vol. 9 No. 1 (2025): February 2025: Pages 1-66
Publisher : Institute for Research and Community Services Udayana University (LP2M Universitas Udayana)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/ATBES.2025.v09.i01.p02

Abstract

This research is motivated by chilies, which are one of the plant products that become raw materials for daily needs and are therefore classified as a secondary food source. Insect pests are a factor affecting chilli productivity, use of fungi as biological controllers, to determine the use of the fungus Beauveria bassiana as a biological control against the insect pest of chilli aphids Myzus persicae Sulz, the research method used in this study is an experimental method using the complete randomized design (CRD) method with five treatments and three replications, where each plot has five samples, the treatment doses were Control, P1 (6 g/500 ml), P2 (7 g/500 ml), P3 (8 g/500 ml), P4 (9 g/500 ml) and Lethal Dose 50, the results of this study showed that the application of B. bassiana had a significant effect on the mortality of M. persicae Sulz with the mortality obtained was 0.75% of the total number of samples 120 outside the control treatment, so the higher the dose, the higher the mortality, the LD50 result was 6.025 g/ml, showing that the toxicity of B. bassiana falls into the category of mild toxins. Therefore, we can conclude that the fungus B. bassiana serves as an effective biological control agent against the insect pest M. persicae Sulz.
Efektivitas Serbuk Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera) terhadap Kadar Glukosa Darah Tikus Putih (Rattus norvegicus L.) Hiperglikemia Elsi Elsi; Iriani Setyawati; Nonny Manampiring; Yermia Semuel Mokosuli; Anita Constanci Christine Tengker; Helen Joan Lawalata
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p6

Abstract

Dua puluh tujuh ekor tikus putih (Rattus norvegicus L.) jantan dibagi menjadi 9 kelompok perlakuan yaitu kontrol normal (K1), kontrol negatif (K2), kontrol positif (K3), serbuk daun kersen 800 mg/kgB (K4), serbuk daun kelor 800 mg/kgBB (K5), serbuk daun kersen 1600 mg/kg BB (K6), serbuk daun kelor 1600 mg/kgBB (K7), kombinasi serbuk daun kersen 400 mg/kgBB dan daun kelor 400 mg/kg BB (K8), kombinasi serbuk daun kersen 800 mg/kg BB dan daun kelor 800 mg/kgBB (K9). Pengukuran kadar glukosa darah tikus dilakukan pada hari ke-3, 7, dan 10. Hasil analisis menunjukan bahwa pemberian dosis kombinasi serbuk daun kersen 800 mg/kgBB dan daun kelor 800 mg/kgBB merupakan dosis yang paling efektif dalam menurunkan kadar glukosa darah pada tikus putih jantan
Efektivitas Serbuk Daun Kersen (Muntingia calabura L.) dan Daun Kelor (Moringa oleifera) terhadap Kadar Glukosa Darah Tikus Putih (Rattus norvegicus L.) Hiperglikemia Elsi Elsi; Iriani Setyawati; Nonny Manampiring; Yermia Semuel Mokosuli; Anita Constanci Christine Tengker; Helen Joan Lawalata
Metamorfosa: Journal of Biological Sciences Vol. 11 No. 01 (2024)
Publisher : Program Magister Ilmu Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2024.v11.i01.p6

Abstract

Dua puluh tujuh ekor tikus putih (Rattus norvegicus L.) jantan dibagi menjadi 9 kelompok perlakuan yaitu kontrol normal (K1), kontrol negatif (K2), kontrol positif (K3), serbuk daun kersen 800 mg/kgB (K4), serbuk daun kelor 800 mg/kgBB (K5), serbuk daun kersen 1600 mg/kg BB (K6), serbuk daun kelor 1600 mg/kgBB (K7), kombinasi serbuk daun kersen 400 mg/kgBB dan daun kelor 400 mg/kg BB (K8), kombinasi serbuk daun kersen 800 mg/kg BB dan daun kelor 800 mg/kgBB (K9). Pengukuran kadar glukosa darah tikus dilakukan pada hari ke-3, 7, dan 10. Hasil analisis menunjukan bahwa pemberian dosis kombinasi serbuk daun kersen 800 mg/kgBB dan daun kelor 800 mg/kgBB merupakan dosis yang paling efektif dalam menurunkan kadar glukosa darah pada tikus putih jantan
Co-Authors Alfonds Andrew Maramis Anice I. Tuda Anita Constanci Christine Tengker Anita Constanci Christine Tengker Anita Costanci Christine Tengker Arrijani - Britney Meissy Pandoh Budiarto Buyang, Dalle Carolin Manuahe Danny Christian Posumah Decky D. W. Kamagi Dewa Nyoman Oka Dina Munawaroh Dina Rombot Djaila, Friscila Avianti Djarang, Rievo H Edieli Zebua Edyson Maneasa Elsi Elsi Emma M Moko Esibrena Br Kemit Eurika, Uwuh Jessie Fadly Steven Jefry Rumondor Fanny Nella Nanlohy Febiola Gabriela Tular Febiola Najoan Feby Febrianty Toding Rante Ferencia Esananda Rattu Ferni Margo Tumbel Ferny Margo Tumbel Feydina Tewu Friska Mery Montolalu Futwembun, Fransiska Gedoan, Sukmarayu Piter Grace Jenny Soputan H. M. Sumampouw Hariyadi Hariyadi Helen Joan Lawalata Helen Joan Lawalata Helen Joan Lawalata Hendra Pratama Maliangkay Hermanus, Britney Putri Herry Maurits Sumampouw Herry Maurits Sumampouw Ilham Saputra Butar-butar Iriani Setyawati Iriani Setyawati IRIANI SETYAWATI Jacklin Stella Manopo Jantje Ngangi Jantje Ngangi Jantje Pelealu Jeane Mantiri Jimmy Langi Jonathan Polandos julduz ruland paus Kamagi, Decky D. W. Kandowangko, Sandy Kaunang, Eva S. N. Maidangkay, Vonny Mamuaya, Tripena Manampiring, Noni Manampiring, Nonny Mansyur, Djailani Masje Wurarah Masje Wurarah Masye Wurarah Max Tulung Megawati Meylin Weken Meike Paat Meisa Tabita Rogahang Meity Nelltje Tanor Melita Irene Gracia Kaligis Meyer Worang Matey Migau, Natalina Mongdong, Priskila Indah Theresya Motulo, Serlin J Mulder, Keysia Stephanie Tesalonica Mundaeng, Camel Gantonio Nerni Potolangi Nonny Manampiring Nova L.I.M Ogi Ogi, Nova LIM Orbanus Naharia Orbanus Naharia Patandean, Selvrining Pomalingo, Moh Fikri Pratasik, Stralen Pratasik, Veronika Pua, Devis Christian Rahardiyan, Dino Revolson Mege Rolef Rumondor Rudi Alexander Repi Rymond Jusuf Rumampuk Semuel Harpandi Sibala, Henike Simbolon, Kristina Br Sinyo Herry Sumampouw Suddin Simandjuntak Sukmarayu Gedoan Sukmarayu P. Gedoan Sukmarayu Pieter Gedoan Sumampouw, Harry Maurits Taariwuan, Marlin Bernadet Thesia Megi Kurniawati Rettob Timbuleng, Nobel Tjiakra, Rosalina A. Tuegeh, Reinny Silvana Tumanduk, Yahya Y.R. Turambi, James S. Utari Satiman Utari Satiman Verawati I Y Roring Verawati Ida Yani Roring Verra E. J. Mawitjere Wisda Nurjannah Abdullah Worang, Rina L. Wuwungan, Axel Yessie K. Kelly