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All Journal MEDIA MEDIKA INDONESIANA Jurnal Berkala Epidemiologi Berkala Ilmu Kesehatan Kulit dan Kelamin Jurnal NERS Indonesian Journal of Tropical and Infectious Disease Jurnal Pengabdian Kepada Masyarakat (Indonesian Journal of Community Engagement) The Indonesian Journal of Public Health The Indonesian Biomedical Journal JI-KES (Jurnal Ilmu Kesehatan) JUXTA: Jurnal Ilmiah Mahasiswa Kedokteran Universitas Airlangga Jurnal Abdi Insani JOURNAL OF COMMUNITY MEDICINE AND PUBLIC HEALTH RESEARCH Indian Journal of Forensic Medicine & Toxicology Jurnal Ilmiah Kesehatan (JIKA) COMSERVA: Jurnal Penelitian dan Pengabdian Masyarakat Asian Journal of Health Research Archives of Pediatric Gastroenterology, Hepatology, and Nutrition JI-KES (Jurnal Ilmu Kesehatan) Folia Medica Indonesiana
Alpha Fardah Athiyyah
Departemen Ilmu Kesehatan Anak RSU Dr. Soetomo Universitas Airlangga, Jl. Mayjen Prof. Dr. Moestopo 6-8, Surabaya
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Journal : MEDIA MEDIKA INDONESIANA

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Proteksi Probiotik pada Mukosa Ileum Mencit yang Terpajan Lipopolisakarida Escherichia Coli Alpha Fardah Athiyyah; Ariani Setiawati; Andy Darma; Anang Endaryanto; I Ketut Sudiana; Reza Ranuh; Subijanto MS
MEDIA MEDIKA INDONESIANA 2012:MMI VOLUME 46 ISSUE 2 YEAR 2012
Publisher : MEDIA MEDIKA INDONESIANA

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Abstract

Probiotic’s protection in ileal mucosa of mice after lipopolysaccaride Escherichia coliBackground: Gastrointestinal infection is one of the major causes of morbidity in children. Diarrhea is the common manifestation. Diarrheal prevention needs a balance mucosal immune system. Probiotic used in prevention of gastrointestinal infection needs to be considered. The aim of the study is to prove probiotic protection in ileal mucosa after lipopolysaccaride (LPS) Escherichia coli O55:B5 inoculation anatomically and immunologically.Method: Experimental study with male Balb/c, age 10-12 weeks, body weight 30-40g and randomized into treatment group and placebo group. Treatment group received mix probiotic for 14 days and on day 15 were inoculated by LPS Escherichia coli O55:B5, and day 16-21 were given mix probiotic again. Placebo group received LPS Escherichia coli O55:B5 on day 15. Mice necropsy were conducted at day 22. Immunohistochemistry examination used to look for amount expression cell for IL-2, IFN-γ, TNF-α, IL-4, IL-5,IL-6, IL-10 dan TGF-β in ileal mucosa. Scanning electron microscope was used to see ileal mucosal structure. Statistic analysis used in this study was multivariate analysis.Results: Sixteen Balb/c mices were randomized into 2 groups, each group consist 8 mices. There was significant difference on amount of cells expression IL-5 (p=0.022), IL-6 (p=0.05), and also on amount of cells expression TGF-β (p=0.036). On probiotic- LPS group, there was no Th1 domination but on the other hand, Treg became dominant. Th1 and Th2 response were still balance. Structural damages occurred in LPS group and did not occur in probiotic-LPS group.Conclusion: Probiotic protection in ileal Balb/c mice mucosa after LPS Escherichia coli O55:B5 inoculation is through anatomy and immunology changes.Keywords: Probiotic, LPS, Escherichia coli, protection, immunologyABSTRAKLatar belakang: Infeksi gastrointestinal dengan manifestasi tersering diare merupakan salah satu infeksi yang sering terjadi pada anak. Pencegahan diare memerlukan keseimbangan sistem imun mukosa yang baik. Penggunaan probiotik untuk pencegahan terhadap infeksi gastrointestinal perlu dipikirkan. Penelitian ini bertujuan untuk membuktikan proteksi probiotik pada ileum mencit yang terpajan lipopolisakarida (LPS) Escherichia coli O55:B5 secara anatomis dan imunologis.Metode: Uji eksperimental dengan menggunakan mencit Balb/c jantan berusia 10-12 minggu, berat badan 30-40g dan dirandomisasi menjadi kelompok perlakuan dan kelompok kontrol. Kelompok perlakuan mendapatkan mix probiotik selama 14 hari, hari ke-15 mendapatkan LPS Escherichia coli O55:B5 dan hari ke-16-21 kembali mendapatkan mix probiotik. Kelompok kontrol mendapatkan LPS Escherichia coli O55:B5 saja pada hari ke-15. Seluruh mencit dinekropsi pada hari ke-22. Pemeriksaan imunohistokimia untuk melihat ekspresi sel penghasil sitokin IL-2, IFN-, TNF-α, IL-4, IL-5, IL-6, IL-10 dan TGF-ß di jaringan ileum. Scanning microscope electron (SEM) untuk melihat struktur mukosa ileum. Analisis statistik yang digunakan adalah analisis multivariat.Hasil: Enam belas ekor mencit Balb/c terbagi menjadi dua kelompok, masing-masing 8 mencit. Pada kelompok probiotik-LPS didapatkan perbedaan bermakna dibandingkan dengan kelompok LPS pada jumlah penghasil sitokin IL-5 (p=0,022), IL-6 (p=0,050) dan jumlah sel penghasil sitokin TGF-β (p=0,036). Pada kelompok probiotik-LPS, dominasi respon Th1 tidak terlihat lagi dan menimbulkan dominasi Treg pada kelompok ini. Posisi Th1 dan Th2 masih dapat dipertahankan secara seimbang. Kerusakan struktur yang terjadi pada kelompok LPS tidak didapatkan pada kelompok Probiotik-LPS.Simpulan: Proteksi probiotik pada mukosa ileum yang terpajan LPS Escherichia coli terjadi secara anatomi dan imunologi.
Co-Authors Adhiningsih, Yunita Ratri Adiba, Asmahan Farah Adinda Juwita Syakila Elizafanti Afif Nurul Hidayati Ana Bilah, Aisah Nur Anak Agung Putri Nadia Paramitha Anang Endaryanto Andro Pramana Witarto Andy Darma Antonio Ayrton Widiastara Ariani Setiawati Asmahan Farah Adiba Budi Utomo Budiono Budiono Budiono Budiono BUDIYANTO Cahyanti, Ika Yuniar Christian Susianto, Steven Cita Rosita Sigit Prakoeswa Dadik Rahardjo, Dadik Dadik Raharjo Deasy Fetarayani Desiana Widityaning Sari Dewi Retno Suminar Dwi Murtiastutik Dwiyanti Puspitasari, Dwiyanti DYAH FAUZIAH, DYAH Eko Budi Koendhori, Eko Budi Ergia Latifolia Faradiba, Ailsa Reina Farihazqa Hafez Mikala Fatimah, Nurmawati Ferry Sandra Fitria, Anisa Lailatul Fitriah Gabriel Pedro Mudjianto Gatot Soegiarto Grahana Ade Candra Wolayan Gunawan, Inggil Mukti Hariastawa, I Gusti Bagus Adria Hengki Anggara Putra Heny Arwati Hidajat, Boerhan I Gusti Made Reza Gunadi Ranuh I.G.B. Adria Hariastawa Indah Setyawati Tantular, Indah Setyawati Ingrid Suryanti Surono INGRID SURYANTI SURONO Insyira Rayhana Irsan Irsan, Insyira Rayhana Juniastuti Juniastuti Kasiyama Desi Indriyani Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Khadijah Rizky Sumitro Konstantin, Tiffany Laura Navika Yamani Lilik Djuari Linda Astari, Linda Linda Dewanti M. Yulianto Listiawan Maharani, Aussie Tahta Maria Inge Lusida Martha Indah Widia Ningtyas Mira Irmawati Muhammad Faizi Muhammad Faizi, Muhammad Muhammad Irawan Muhammad Miftahussurur Nesa, Ni Nyoman Metriani Ni Nyoman Metriani Nesa Ni Nyoman Metriani Nesa Novi Rahayu Arianti Nur Aisiyah Widjaja, Nur Aisiyah PA, Deanty Ayu PA, Deanty Ayu Raharjo, Dadik Retno Asih Setyoningrum Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Gunadi Ranuh Reza Ranuh Reza Ranuh Rohmah, Etik Ainun Ronald Pratama Adiwinoto Rossyanti, Lynda S.Pd. M Kes I Ketut Sudiana . Sacharissa Zerlina Tsarwah Thirafi Sawitri Sawitri Shirakawa, Toshiro Shirakawa, Toshiro SITI NURUL HIDAYATI Sitti Khofifah Yuliana Soedarmo, Subijanto Marto Sofia Wardhani Sriwijayanti Sulistyawati Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Steven Christian Susianto Subijanto Marto Soedarmo Subijanto MS Subijanto Subijanto Sudarmo, Subijanto Marto Suharno, Novianto Edi Suhintam Pusarawati Sukmawati Basuki Sulistiawati Sulistiawati Sulistiawati Sumitro, Khadijah Rizky Susianto, Steven Christian Tiffany Konstantin Tiffany Konstantin Toshiro Shirakawa Vina Lidya Setjaputra Wibi Riawan Widati Fatmaningrum Wiweka Merbawani Wolayan, Grahana Ade Candra Yetti Hernaningsih Yunita Ratri Adhiningsih Zayyin Dinana Zunnahri, Salsabila Qurrotul Aini