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Ekstrak Daun Salam (Eugenia polyantha, wight.) Sebagai Pengawet Alami Daging Ayam Broiler di Pasar Wonokromo Hidayah, Nurul; Kusuma Wardhani, Lailia Dwi; Putri Ekapaksi, Caecilia Cindy; Wibisono, Freshinta Jellia
VITEK : Bidang Kedokteran Hewan Vol. 11 No. 2 (2021): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v11i2.84

This study aimed to determine the effect of bay leaf extract (Eugenia Polyantha, Wight.) as a natural preservative for broiler chicken meat in the Wonokromo market. The experimental design used in this study was a completely randomized design with 4 treatments and 5 replications, namely P0 (control), P1 (bay leaf extract 5%), P2 (15% bay leaf extract) and P3 (25% bay leaf extract). Broiler chicken thighs were soaked for 30 minutes and stored at room temperature (20-25OC) for 24 hours. The organoleptic test processed descriptively and the panelists was the researchers herself. The color of the best broiler chicken meat was at P3 with a brownish yellow color, the best broiler chicken meat smell was P3 with a slightly rotten smell and a bit of bay leaf aroma and the best broiler chicken meat texture was 2, namely in P2 and P3 with a slightly soft texture. The initial test of decay (Eber test) and total bacterial colonies (TPC) used statistical analysis of analysis of variance data. The mean initial rot (Eber) was at P0 1.00, P1 0.83, P2 0.33, P3 0.17. The average total bacterial colonies (TPC) were at P0 3.35 x 106, P1 3.03 x 106, P2 2.71 x 106, P3 2.50 x 106. The results of statistical analysis showed that bay leaf extract had a significant effect on early rot (Eber) and total bacterial colonies. (TPC) of broiler chicken in the Wonokromo market results was (P<0.05), although the total number of bacterial colonies (TPC) has exceeded the maximum TPC limit according to SNI No.3924-2009 which achieved 1x106/cfu. The conclusion of this study was that the concentration of 25% bay leaf extract produced the best durability in broiler chicken meat with organoleptic results of a brownish yellow color, a slightly rotten odor and a slight aroma of bay leaf and also a slightly soft texture, the initial value of rottenness (Eber test) was 0.17 ± 0.40 and total value of bacterial colonies 2.50 x 106 ± 0.57 x 106/cfu. .

Efektivitas Antibakteri Ekstrak Daun Sisik Naga (Drymoglossum piloselloides) Terhadap Bakteri MRSA (methicilin resistant Staphylococcus aureus) dan Eschericia coli Wirjatmadja, Roeswandono; Kurniasari, Putri Nadia Indah; Wibisono, Freshinta Jellia; Kurnianto, Ady
VITEK : Bidang Kedokteran Hewan Vol. 12 No. 2 (2022): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v12i2.112

The purpose of this research aimed to determine the antibacterial effectiveness of the methanolic extract of dragon scales leaves (Drymoglossum piloselloides) against MRSA (Methicilin-resistant Staphylococcus aureus) and Eschericia coli bacteria in vitro using the diffusion method. This research used a population of Methicillin-resistant Staphylococcus aureus and Eschericia coli bacteria. The sample to be used is dragon scale leaf methanol extract. The method in this research used experimental with 6 treatments, namely dragon scale leaf methanol extract with concentrations of 45%, 55%, 65%, 75%, negative control (methanol) and positive control (chloramphenicol antibiotics). Data collection in this study was carried out by recording the diameter of the inhibition zone using a caliper (mm). The data obtained from the diameter of the inhibition zone of 75% extract variation resulted in an inhibition zone of 17.02 mm (in MRSA) and 8.47 mm (in E.coli). Chloramphenicol was found to be resistant to MRSA, but sensitive to E.coli. Data analysis used ANOVA test which statistically showed that there was a significant difference (P<0.05) in the diameter of the inhibition zone of the dragon scale leaf methanol extract against MRSA bacteria and there was a very significant difference (P<0.01) in the diameter Inhibition zone of dragon scale leaf methanol extract against Eschericia coli bacteria.

Pengaruh Ekstrak Daun Salam (Eugenia polyantha, weight.) Sebagai Alternatif Mempertahankan Kualitas Daging Ro Candra, Adhitya Yoppy; Yanestria, Sheila Marty; Mardijanto, Arief; Wibisono, Freshinta Jellia
VITEK : Bidang Kedokteran Hewan Vol. 12 No. 2 (2022): VITEK-Bidang Kedokteran Hewan
Publisher : Fakultas Kedokteran Hewan Universitas Wijaya Kusuma Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30742/jv.v12i2.120

One of the good sources of animal protein comes from meat because it contains various kinds of nutrients needed by the body including protein, carbohydrates, water, vitamins, fats and various essential amino acids. The presence of high nutritional value in meat can also be utilized by various microorganisms to grow so that it can reduce the quality of meat. Some of these microorganisms can be transmitted to humans and cause disease or also known as zoonotic diseases. One way to prevent the growth of microorganisms in meat is by preserving meat using natural ingredients that can come from plants. One of the plants that has the potential to inhibit bacterial growth is Salam leaves (Eugenia polyantha, Weight.). The purpose of this study was to determine the value of the content in Salam Leaves (Eugenia polyantha, Weight.) and to determine the effect of giving Salam Leaf extract (Eugenia polyantha, Weight.) to early decay, pH value and Total Bacterial Colony. The study began with the collection of Salam leaves (Eugenia polyantha, Weight.) and then the extract was made. The extract obtained was then diluted and stored. The research was continued by taking meat samples from the Traditional Market in Surabaya and then cut into uniform sizes and soaked in Salam leaf extract with different concentrations. After incubation, the meat quality was tested for the beginning of spoilage, pH value and total bacterial colonies. The results of the observations showed that the best results were found in the P4 treatment with an extract concentration of 20%. This result is indicated by the lack of fog formed in the initial spoilage test, the pH value is close to the ultimate pH of the meat and a low total bacterial colony.

Status Reproduksi Ternak dan Pelayanan Kesehatan Hewan Paska Penyakit Mulut dan Kuku di Kecamatan Jatinom Klaten: Livestock Reproduction Status and Animal Health Services After Foot and Mouth Disease in Jatinom District Klaten Wibisono, Freshinta Jellia; Candra, Adhitya Yoppy Ro; Wirjaatmadja, Roeswandono
IGKOJEI: Jurnal Pengabdian Masyarakat Vol. 5 No. 1 (2024): IGKOJEI: Jurnal Pengabdian Masyarakat
Publisher : Fakultas Peternakan Universitas Papua

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46549/igkojei.v5i1.442

ABSTRACT Foot and mouth disease is a disease that has a big impact on economic sector of livestock farmers. Foot-and-mouth disease outbreaks result in increased livestock deaths, decreased livestock production, body weight, and livestock reproductive health. Cattle are one of the livestock animals that support meat self-sufficiency and also included in the ruminant livestock group. It can convert low nutritional value feed into high nutritional value food. The meat demand continues increase over tim, thus it needs to be supported by an adequate increase in livestock population. The good reproductive status checks and adequate health services for livestock are needed to ensure the livestock population. This community service regarding the reproductive health status of livestock is important to provide education to the farmers. This community service targets livestock groups in Jatianom District, Klaten Regency. The results showed the reproductive status of livestock in the Tani Makmur and Lembu Suro groups, were quite good. Implementation of communication, information and education on farmers' understanding of foot-and-mouth disease can be carried out as an effort to prevent and treat reproductive disorders in brooms with the aim of increasing pregnancy rates, reproductive performance and reducing livestock mortality rates. Keywords: Animal health; Cattle; Disease ABSTRAK Penyakit mulut dan kuku merupakan penyakit yang memiliki dampak yang cukup besar pada peternak, terutama dibidang ekonomi. Wabah penyakit mulut dan kuku berdampak pada peningkatan kematian ternak, penurunan produksi ternak, penurunan berat badan, dan penurunan kesehatan reproduksi ternak. Ternak sapi merupakan salah satu hewan ternak yang dapat dipelihara secara intensif guna mendukung swasembada daging. Sapi juga termasuk kedalam kelompok ternak ruminansia yang dapat mengubah pakan yang memiliki nilai nutrisi rendah menjadi bahan pangan yang memiliki nilai nutrisi tinggi. Kebutuhan akan daging terus meningkat seiring berjalannya waktu sehingga perlu ditunjang dengan peningkatan populasi ternak yang memadai. Namun kebutuhan akan peningkatan populasi sapi perlu ditunjang dengan pemeriksaan status reproduksi yang baik serta pelayanan kesehatan yang memadai pada ternak. Sehingga pengabdian masyarakat mengenai status kesehatan reproduksi ternak, penting dilakukan untuk memberikan edukasi kepada masyarakat peternak. Pengabdian kepada masyarakat ini menyasar pada kelompok ternak yang berada di Kecamatan Jatianom, Kabupaten Klaten. Dari hasil pemeriksaan status reproduksi ternak pada kelompok Tani Makmur dan Lembu Suro menunjukkan hasil reproduksi yang cukup baik. Pelaksanaan komunikasi, informasi, dan edukasi pada pemahaman peternak terhadap penyakit mulut dan kuku dapat dilakukan sebagai upaya untuk pencegahan dan penanganan gangguan reproduksi pada sapi yang bertujuan dalam upaya peningkatan tingkat kebuntingan, performa reproduksi dan penurunan tingkat kematian ternak. Kata kunci: Kesehatan hewan; Penyakit; Sapi

Pemeriksaan Helminthiasis Pada Ayam Layer Di Baim Farm Firdasari, Andrianti; Wibisono, Freshinta Jellia; Najwan, Rifqi
Prosiding Seminar Nasional Kusuma Vol 3 (2025): Prosiding Seminar Nasional Kusuma
Publisher : LPPM UWKS

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Latar belakang: Helminthiasis merupakan salah satu masalah kesehatan utama pada peternakan ayam layer yang dapat menurunkan efisiensi pakan, pertumbuhan, dan produksi telur. Infeksi ini disebabkan oleh cacing dari kelas nematoda dan cestoda, terutama Heterakis gallinarum dan Ascaridia galli. Tujuan: Untuk mengidentifikasi jenis helminth yang menginfestasi ayam layer serta mengevaluasi faktor risiko yang mempengaruhi kejadian infeksi di Baim Farm Tuban. Metode: Melakukan observasi manajemen pemeliharaan, wawancara dengan peternak, serta melakukan nekropsi terhadap 10 ekor ayam layer yang mati untuk mengidentifikasi jenis cacing pada saluran pencernaan. Hasil: Dari 10 sampel ayam layer, terdapat 60% terinfestasi cacing heterakis gallinarum sebanyak 40%, dan raillitina tetragona 10%, sedangkan kelas trematoda tidak ditemukan. Kesimpulan: biosekuriti dan manajemen pemeliharan ayam layer merupakan faktor penting sebagai upaya pencegahan infestasi keberadaan cacing dalam saluran pencernaan.

Patologi Anatomis Organ Hepar Ayam Yang Terindentifikasi Penyakit Berak Kapur Frinandar, Dwi Laksono; Wibisono, Freshinta Jellia; Djapri, Ione Novibrina
Prosiding Seminar Nasional Kusuma Vol 3 (2025): Prosiding Seminar Nasional Kusuma
Publisher : LPPM UWKS

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Latar Belakang: Penyakit berak kapur merupakan penyakit menular pada unggas yang disebabkan oleh Salmonella pullorum. Penyakit ini banyak menyerang anak ayam dan dapat menyebabkan angka kematian tinggi. Salah satu organ target utama dari infeksi Salmonella pullorum adalah hepar, yang berperan penting dalam metabolisme dan detoksifikasi. Pemeriksaan terhadap perubahan patologi anatomis hepar dapat memberikan informasi penting mengenai tingkat keparahan dan perjalanan penyakit. Tujuan: Pemeriksaan ini bertujuan untuk mendeskripsikan perubahan patologi anatomis pada organ hepar ayam Metode: Sampel hepar diambil dari ayam yang menunjukkan gejala klinis khas penyakit berak kapur seperti diare putih, lemas, dan pertumbuhan terhambat. Pemeriksaan dilakukan secara makroskopis meliputi pengamatan warna, ukuran, konsistensi, serta kelainan morfologis pada permukaan organ. Hasil: Hasil pengamatan menunjukkan bahwa hepar ayam yang positif Salmonella pullorum mengalami pembesaran (hepatomegali), perubahan warna menjadi pucat kekuningan, serta terdapat bercak nekrosis multifokal berwarna putih keabu-abuan pada permukaan lobus hepar. Pada beberapa sampel ditemukan pula kongesti dan degenerasi lemak. Gambaran ini menunjukkan adanya kerusakan sel hepatosit akibat infeksi sistemik bakteri. Kesimpulan: Perubahan patologi anatomis pada hepar ayam penderita penyakit berak kapur ditandai oleh hepatomegali, perubahan warna, dan lesi nekrotik multifokal. Hasil ini memperkuat pemahaman mengenai patogenesis infeksi Salmonella pullorum serta dapat dijadikan dasar dalam diagnosis dan pengendalian penyakit berak kapur di lapangan.

Uji Residu Dan Pemeriksaan Total Bakteri Pada Ayam Broiler Di Pasar Tradisional Kota Surabaya Elfrian, Fadly; Wibisono, Freshinta Jellia; Fitria, Siti Lailatul
Prosiding Seminar Nasional Kusuma Vol 3 (2025): Prosiding Seminar Nasional Kusuma
Publisher : LPPM UWKS

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Latar Belakang: Daging ayam broiler merupakan sumber protein hewani yang populer di Indonesia. Peningkatan permintaan pasar memicu peternakan intensif yang seringkali menggunakan antibiotik (misalnya Tetrasiklin, Sulfonamida, β-laktam, Makrolida, Aminoglikosida) sebagai bagian dari manajemen kesehatan. Penggunaan yang tidak tepat dosis dan mengabaikan waktu henti (withdrawal time) dapat menyebabkan residu antibiotik pada daging. Residu ini berisiko menimbulkan alergi, gangguan mikrobiota usus, dan, yang paling mengkhawatirkan, resistensi antimikroba pada manusia, yang telah ditetapkan WHO (2024) sebagai ancaman kesehatan global. Selain itu, mutu daging dinilai dari cemaran mikroba melalui uji Total Plate Count (TPC), dengan batas maksimum SNI sebesar 1 × 10⁶ CFU/ gram. Tujuan: Penelitian deskriptif kuantitatif ini bertujuan untuk mengidentifikasi dan menentukan tingkat kejadian residu antibiotik serta nilai TPC pada daging ayam broiler di pasar tradisional Surabaya Timur. Metode: Pengambilan 30 sampel daging ayam (dada dan paha) dilakukan secara acak dari 5 pasar tradisional. Residu antibiotik dideteksi menggunakan metode Bioassay Mikrobiologi (Inhibition Test) dengan bakteri indikator Bacillus subtilis. TPC diukur dengan metode Pour Plate mengacu pada standar ISO 4833-1:2013. Hasil: Berdasarkan hasil pengujian yang sudah dilakukan diperoleh hasil bahwa 96.67% (29/30) dari sampel daging yang diperiksa terdapat residu antibiotik. Hasil uji Total Plate Count (TPC) juga memperlihatkan variasi jumlah koloni bakteri aerob mesofilik yang signifikan, dengan nilai berkisar antara 3,0 × 10² hingga 4,0 × 10⁶ CFU/g. Kesimpulan: Keberadaan residu antibiotik dan nilai TPC yang tinggi secara bersamaan menunjukkan potensi risiko ganda bagi konsumen, baik dari aspek kimiawi maupun mikrobiologis. Diperlukan pengawasan yang lebih ketat terhadap penggunaan antibiotik di peternakan serta peningkatan higiene dan sanitasi di pasar tradisional sebagai upaya pengendalian risiko kesehatan masyarakat veteriner.

Dinamika Epidemiologi Multidrug Resistensi (Ampicilin, Streptomycin and Tetracycline) pada Kucing Domestik di Wilayah Kota Surabaya Timur Wibisono, Freshinta Jellia; Qurratu’ain, Seryna Hasna; Jalal, Ismul; Fardiansyah, Al; Isnaeni, Mutia; Mentari, Andi Oktaviana; Islam, Afif Fajrul; Wibisono, Freshindy Marissa; Widiasih, Dyah Ayu; Freshinta Jellia Wibisono
Acta VETERINARIA Indonesiana Vol. 13 No. 3 (2025): November 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.3.248-258

Antibiotic resistance in pets, especially cats, has become a serious global health challenge. The irrational use of antibiotics, whether excessive or inappropriate, accelerates the emergence of resistant bacteria that can potentially spread to humans and the environment. This study aims to identify the presence of antibiotic-resistant Escherichia coli and analyze its resistance patterns in domestic cats in East Surabaya. A total of 35 rectal swab samples were collected from pet cats and tested at the Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya. The samples were isolated using the selective MacConkey Agar medium and confirmed as Escherichia coli through Gram staining, biochemical tests, and antibiotic susceptibility testing. The results showed that 94.3% (33/35) of the samples contained Escherichia coli. Antibiotic susceptibility testing revealed resistance rates of 27% (9/33) to ampicillin, 24% (8/33) to streptomycin, and 12% (4/33) to tetracycline. Additionally, 9.1% (3/33) of the isolates were classified as multidrug resistance, showing resistance to all three antibiotics (AMP-TE-S). Other resistance patterns included AMP-S (6.1%) and TE-S (3.1%). The high resistance rate of Escherichia coli in domestic cats highlights the critical role of pets as potential reservoirs of resistant bacteria, which may impact human health through direct contact or environmental transmission. Therefore, raising awareness and educating pet owners and veterinary professionals on the prudent and responsible use of antibiotics is essential. The One Health approach, integrating human, animal, and environmental health, is a strategic measure to prevent the future spread of antimicrobial resistance.

Dinamika Epidemiologi Multidrug Resistensi (Ampicilin, Streptomycin and Tetracycline) pada Kucing Domestik di Wilayah Kota Surabaya Timur Wibisono, Freshinta Jellia; Qurratu’ain, Seryna Hasna; Jalal, Ismul; Fardiansyah, Al; Isnaeni, Mutia; Mentari, Andi Oktaviana; Islam, Afif Fajrul; Wibisono, Freshindy Marissa; Widiasih, Dyah Ayu; Freshinta Jellia Wibisono
Acta VETERINARIA Indonesiana Vol. 13 No. 3 (2025): November 2025
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/avi.13.3.248-258

Antibiotic resistance in pets, especially cats, has become a serious global health challenge. The irrational use of antibiotics, whether excessive or inappropriate, accelerates the emergence of resistant bacteria that can potentially spread to humans and the environment. This study aims to identify the presence of antibiotic-resistant Escherichia coli and analyze its resistance patterns in domestic cats in East Surabaya. A total of 35 rectal swab samples were collected from pet cats and tested at the Veterinary Public Health Laboratory, Faculty of Veterinary Medicine, Wijaya Kusuma University, Surabaya. The samples were isolated using the selective MacConkey Agar medium and confirmed as Escherichia coli through Gram staining, biochemical tests, and antibiotic susceptibility testing. The results showed that 94.3% (33/35) of the samples contained Escherichia coli. Antibiotic susceptibility testing revealed resistance rates of 27% (9/33) to ampicillin, 24% (8/33) to streptomycin, and 12% (4/33) to tetracycline. Additionally, 9.1% (3/33) of the isolates were classified as multidrug resistance, showing resistance to all three antibiotics (AMP-TE-S). Other resistance patterns included AMP-S (6.1%) and TE-S (3.1%). The high resistance rate of Escherichia coli in domestic cats highlights the critical role of pets as potential reservoirs of resistant bacteria, which may impact human health through direct contact or environmental transmission. Therefore, raising awareness and educating pet owners and veterinary professionals on the prudent and responsible use of antibiotics is essential. The One Health approach, integrating human, animal, and environmental health, is a strategic measure to prevent the future spread of antimicrobial resistance.

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