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Ersalina Nidianti
Universitas Nahdlatul Ulama Surabaya, Indonesia
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Journal : INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY

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CYTOTOXICITY ASSAY USING BRINE SHRIMP LETHALITY TEST ON COLLAGEN-CHITOSAN WOND DRESSING STERILIZED BY ULTRAVIOLET LIGHT Ary Andini; Endah Prayekti; Devyana Dyah Wulandari; Ersalina Nidianti
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 2 No 1 (2020): Laboratory Examinations Support in Medical Toxicology
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v2i1.1467

Abstract

Collagen gives a moist state on the wound area to accelerate the wound healing process. Chitosan is a polymer as known as non-toxic, antibacterial, antifungal, biodegradable, and biocompatible materials. Combination of collagen and chitosan expected to be the best biomaterials as a wound dressing for the healing process. The study aimed to determine cytotoxicity assay on collagen-chitosan wound dressing sterilized by ultraviolet (UV) Light using Brine Shrimp Lethality Test (BSLT) method. The test groups were divided into K0, K1, K2, and K3 groups. K0 contained pure chitosan as a control group, K1 contained collagen 25%-chitosan 75%, K2 contained collagen 50%-chitosan 50%, K3 contained collagen 75%-Chitosan 25%. Collagen extracted from skin and scalp of snakehead fish (Channa striata) then mixed with chitosan until formed collagen-chitosan wound dressing. This study used Brine Shrimp Level Test (BSLT) method with solution concentration 10, 50, 100, 250, 500, 750 and 1000 ppm. Based on the results showed that K0, K1, K2, and K3 group had LC50 >1000 that proven collagen-chitosan wound dressing was non-toxic materials. As conclusion of the study explained composite wound dressing based on collagen-chitosan in all groups that sterilized under UV-Light along 15 minutes was not toxic based on Brine Shrimp Lethality Test showed LC50 >1000.
Co-Authors Adinda Priyanti Dewi Afianti Diah Anjelia Aidilfi Akbar, Dimas Arendra Aini, Fadlilah Nur Aisah Humairoh Algristian, Hafid Andini Devi Rizkiyah andini, ary Andreas Putro Ragil Santoso, Andreas Putro Ragil Ary Andini Ary Andini Aulia, Ilmiah Alvi Nisa Cahyani, Yuliana Dwi Kurnia Chika Nur Azizah Dea Ayu Rahmawati Desi Susanti Devyana Dyah Wulandari Devyana Dyah Wulandari Dewi Masithah, Dewi Endah Budi Permana Putri Endah Prayekti Endah Prayekti Fadlilah Nur Aini Farah Nuriannisa Fitri Aisyah Haddad Gilang Nugraha Halimah Prisilia Herliani Rahmania Sari Hidayat, Muhammad Taufiq Himawan, Toto Ilmiah Alvi Nisa Aulia Intan Oktariza Nuraulya Putri Irmawati Irmawati KARIMAH, AKHLAQUL Kartika Sari, Nathalya Dwi Khusnah, Hidayatul Kombih, Muhammad Fifin Kurniasari, Diyan Wahyu Maat, Suprapto Mardhotillah, Rachma Rizqina Miladiya, Feby Ayu Muhammad Afwan Romdloni Muhammad Taufiq Hidayat Nandasari, Anggy Dwipa Ngiluhtara Aditiya Putri Nila Dwi Utami Nisrina Salsabila Firdaus Nurdinata, Sandra Maulida Nuris Lutfika Nurul Hidayatih Pertiwi, Penti Prayekti, Endah Puspitasari, Fike Putri, Bismi Aisyah Rahma Putri, Ngiluhtara Aditiya Qotrinnada, Nabilah Rahayu Anggraini Rahayu Anggraini Rahmawati Fitri Awalia Rizka Amalia Rizka Amalia Rizkiyah, Mubarokator Rohmah, Wardah Romdloni, Muhmmad Afwan Rukman, Nia Kurniaty Safitri, Hana Salfa Salsabilah Zain Salfa Salsabilah Zain Santoso, Rizma Defva Sari, Herliani Rahmania Sari, Yuni Nur Malita Saumia Khoirunnisa Syadzila Septyanengrum, Rita Siska Wulandari, Siska Siti Basiroh Sri Surya Suciati Suciati, Sri Surya Sunaryo, Merry Suprapto Ma’at Syadzila, Saumia Khoirunnisa Syiddatul Budury Utami, Nila Dwi Utomo, Edi Priyo Widya Putra, Bima Arya Wieke Sri Wulan Wieke Sri Wulan Wulan, Wieke Sri Yauwan Tobing Lukiyono Yuliani, Kartika Yuni Nur Malita Sari Zain, Salfa Salsabilah