Claim Missing Document
Check
Articles

Found 27 Documents
Search

The Anthelmintic Potential of Ethyl Acetate Fraction Berenuk (Crescentia cujete L.) Fruit Against Mortality Haemonchus contortus In Vitro Dyah Ajeng Suhita; Anwar Ma'ruf; Agus Sunarso; Eduardus Bimo Aksono; Dewa Ketut Meles
Journal of Parasite Science (JoPS) Vol. 7 No. 1 (2023): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v7i1.37770

Abstract

This study aims to determine the anthelmintic potency of the ethyl acetate fraction of berenuk fruit (Crescentia cujete L.) on the mortality of the Haemonchus contortus worms in vitro. The method that was used in the research was a post-test only control group design. There were five treatments and each treatment was carried out in four repetitions. The sample used was twenty H. contortus in each treatment for all replications. Observation and recording of H. contortus mortality was carried out at 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes, and when all worms in the petri dish died. Data analysis using ANOVA, followed by Duncan’s Test and Probit analysis. The results showed that the ethyl acetate fraction of berenuk fruit had anthelmintic activity. The conclusion in this study is that the optimal concentration is found in the 0.5% ethyl acetate fraction concentration, the LC50 at each observation time successively is 1.39%; 0.97%; 0.70%; 0.48%; 0.39%; 0.34%; 0.31%; 0.28%; 0.27%; 0.26%; 0.25%; and 0.24%, while LT50 at a concentration of 0.125%; 0.25%; and 0.5% respectively are 1 hour 17 seconds, 41 minutes 42 seconds, and 20 minutes 58 seconds.
Correlation of Concentration Ethyl Acetate Fraction of Calabash Fruit (Crescentia cujete L.) with Mortality Haemonchus contortus Worm In Vitro Dyah Ajeng Suhita; Anwar Ma'ruf; Agus Sunarso; Eduardus Bimo Aksono Herupradoto; Dewa Ketut Meles
Journal of Parasite Science (JoPS) Vol. 6 No. 2 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jops.v6i2.38973

Abstract

The purpose of this study was to ascertain how the concentration of the ethyl acetate component of calabash fruit (Crescentia cujete L.) affected the in vitro mortality of the Haemonchus contortus worm. This study employed a post-test only control group design as its methodology. There were five treatments, with a total of four repetitions. Twenty H. contortus worms were utilized as samples in each treatment throughout all replications. Observation and recording of H. contortus mortality was carried out at 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60 minutes, and when all worms in the petri dish died. Worm mortality was observed by examining whether there was a movement reaction from the worms when given a touch or water droplets at 50°C. Linear regression test was used to assess the data. The resulting linear equation, y = 2.417 + 5.875x, demonstrated that the ethyl acetate component of calabash fruit had an impact on worm mortality. The conclusion of this study is that the increase in the concentration of the ethyl acetate fraction is directly proportional to the mortality of the worm Haemonchus contortus in vitro with an effect value of 93.4%.
Uji Aktivitas Antifungi Ekstrak Daun Sirih (Piper betle L) terhadap Saprolegnia sp. Secara In Vitro [Antifungal Activities Test Of Betel Leaf Extract (Piper betle L) On Saprolegnia sp. By In Vitro] Rahayu Kusdarwati; Dewa Ketut Meles; Ayu Ratnaningtyas
Jurnal Ilmiah Perikanan dan Kelautan Vol. 5 No. 1 (2013): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v5i1.11417

Abstract

Abstract Saprolegniasis is a mycotic disease caused by Saprolegnia sp. that usually attacking wild fish and farming fish. Saprolegnia sp. cause a lot of harm in process of the fish cultivation. Prevention and treatment of the common practice is use chemical drugs, but the use of these chemicals tend to be environmentally unfriendly and there are has karsinogenik effect. Therefore, the use of traditional medicines is one of alternative to control Saprolegnia sp. safer than chemical drugs. Green betel leaf contains phenolic compounds and tannins are efficacious as antifungal agent. This study aims to prove the antifungal activity of extracts of betel leaf (Piper betle L) for Saprolegnia sp., and to know the minimum concentration of betel leaf extract (Piper betle L) as antifungal for Saprolegnia sp. The design of this experiment is used completely randomized design (CRD) with 11 treatments and 3 replications. This study used the dilution method through the Minimum Inhibitory Concentration determination (MIC) and Minimum Fungicidal Concentration (MFC). The concentration of the extract used was 50% (0.5 g/ml), 25% (0.25 g/ml), 12.5% (0.125 g/ml), 6.25% (0.0625 g/ml), 3.13% (0.0313 g/ml), 1.56% (0.0156 g/ml), 0.78% (0.0078 g/ml), 0.39% (0.0039 g/ml), 0.2% (0.002 g/ml) of betel leaf extract. A positive control containing 2 ml of 3% hydrogen peroxide were added fungal suspension until 4 ml Negative control containing 2 ml of 10% DMSO were added fungal suspension until 4 ml. The main parameters in this study is the value of optical density (OD) for MIC (Minimum Inhibitory Concentration) test and the absence of Saprolegnia sp. growing on SDA media for MFC (Minimum Fungicidal Concentration) test. 0.20% (0.002g/ml) concentration of of betel leaf extract is the minimum concentration that can inhibit the growth of Saprolegnia sp. MFC test results showed concentrations of 0.78% (0.0078 g/ml) betel leaf extract is the minimum concentration that can kill Saprolegnia sp.
Kandungan Protein Kasar dan Serat Kasar Pada Kulit Pisang Raja yang Difermentasi dengan Trichoderma viride dan Bacillus subtilis sebagai Bahan Baku Pakan Ikan [ Crude Protein And Fiber Content Of Rough Of Fermented On The King Banana Skins By Trichoderma viride And Bacillus subtilis As Raw Fish Feed] Agustono Agustono; Mohamad Yusuf; Dewa Ketut Meles
Jurnal Ilmiah Perikanan dan Kelautan Vol. 4 No. 1 (2012): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v4i1.11585

Abstract

Abstract Utilization of fish waste as an alternative feed ingredient in the effort to increase the availability of raw materials making up rations. Banana skin is one that has the potential of agricultural waste into alternative feed ingredients, but the nutritional value contained in the banana peel is still low so we need to attempt to improve nutritional content, one of which is fermentation method using Trichoderma viride and Bacillus subtilis in order to improve crude protein and lower content of crude fiber on a banana peel. This study aims to determine the influence of Trichoderma viride on crude protein and crude fiber are fermented banana skin, know the effect of Bacillus subtilis on crude protein and crude fiber are fermented banana skin and know the effect of interaction of Trichoderma viride and Bacillus subtilis of crude protein and fiber fermented banana skin rough. This research method is to experiment with Completely Randomized Design (CRD) as a factorial experimental design. The treatments used consisted of two factors, namely the effect of Trichoderma viride (factor A) and Bacillus subtilis (factor B). Factor A consisted of three dose levels: 0%, 3% and 6%, while factor B consists of three levels ie dose of 0%, 4% and 8%. Each treatment combination was replicated 3 times. The results showed that the use of Trichoderma viride and Bacillus subtilis in fermentation processes plantain skin showed significant differences on crude protein and crude fiber on dry matter (p<0.05).
THE IMMUNOSTIMULAN POTENTIAL OF TENGGULUN (PROTIUM JAVANICUM) LEAVES TOWARDS T CELL CD4+ AND IFNγ SECRETION ON PBMC CHICKEN Jayawardhana, Andi; Puspitasari, Heni; Meles, Dewa Ketut; Koesdarto, Setiawan
Indonesian Journal of Tropical and Infectious Disease Vol. 6 No. 3 (2016)
Publisher : Institute of Topical Disease Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (188.478 KB) | DOI: 10.20473/ijtid.v6i3.2992

Abstract

One of the plants with immunostimulant activity is Tenggulun leaves which contain of flavonoid, like terpenoid. The aim of this research is to find the potential of Tenggulun's leaves extract to have the immunostimulant activities. The potential of immunostimulant activity is identified by the increasing the amount of T-cell CD4+ expression and IFNγ secretion. The research method is conducted through cultured chicken PBMC which is infected by ND virus; it is then treated with Tenggulun's leaves extract with immunostimulant. The result of immunocitochemistry examination CD4+ secretion on PBMC cultures shows how tenggulun is significantly different from the control in the secretion CD4+. The 10μg of tenggulun extract can modulate the T cell CD4+ secretion 68.8±0.83. It is significantly different from K (control) (p<0,05) and treatment group K+, P0, and P1. The examination of IFNγ level using ELISA from tenggulun leaves extract of 10μg doses were inoculated after being infected by ND virus contained immonostimulant potential in increasing the secretion of IFNγ 120.91±6.44. It is significantly different from K-, K+, and P1, yet not significantly different from P0. The content of terpenoid can increase IFNγ secretion on the macrofag cells culture and limfosit cells. 
The Effect of The Gamal Leaf Ethanol Extract on The Growth of Escherichia coli Isolated from Semi Natural Sea Turtle Nest in Boom Beach, Banyuwangi Hutahaean, Ryan David Pandapotan; Meles, Dewa Ketut; Praja, Ratih Novita; Rahmahani, Jola; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 5 No. 2 (2022): October
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol5.iss2.2022.225-233

Abstract

The aim of this study was to identify the antibacterial activity potention of gamal leaf (Gliricidia sepium) ethanol extract against Escherichia coli growth which isolated from Olive Ridley Sea Turtle (Lepidochelys olivacea) semi-natural nest in Boom beach, Banyuwangi. The antibacterial test used disk diffusion method. Oxytetracycline 30 µg (Oxoid CT0041B) was used as the Positive Control (K+). CMC Na 0,5% was used as the Negative Control (K-). The treatment (P1, P2, P3, P4) used various concentrations of gamal leaves ethanol extract, which is 40%, 50%, 60%, and 70%. The study assigned six treatments and four replication. The obtained data were analyzed by ANOVA, then continued with the DUNCAN test if the results showed significant differences (p<0,05). The inhibition result of positive control (K+) was 24,70c ± 7.04; and the negative control was 0,00a ± 0,00. All extract concentration result different inhibition, concentration 1 (P1) was 10,41 b ± 3,10; concentration 2 (P2) 8,14 b ± 0,45; concentration 3 (P3) 8,08 b ± 0,47; and concentration 4 (P4) 8,01b ± 0,29. These results indicated that the gamal leaves ethanol extract has antibacterial activity to inhibit the growth of E. coli isolated from Olive Ridley semi-natural nest in Boom beach, Banyuwangi.
Risk Factors for Feline Otitis in Madiun: A Prospective Study Khasana, Uswatun; Meles, Dewa Ketut; Praja, Ratih Novita; Tyasningsih, Wiwiek; Wibawati, Prima Ayu
Jurnal Medik Veteriner Vol. 6 No. 1 (2023): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol6.iss1.2023.29-34

Abstract

This study aimed to determine the risk factors in cats associated with the incidence of otitis. A total of 169 cat samples were evaluated by filling out questionnaires and interviewing owners, then an examination was carried out to determine symptoms and treatment so that appropriate action could be taken. Data were analyzed descriptively and tested using Chi-Square analysis. As a result, there were 26 (15,4%) cats diagnosed with otitis. The results of the analysis of significant risk factors include bathing frequency, ear hygiene, and maintenance patterns that can increase the incidence of otitis. The most common clinical symptoms are pruritus, head shaking and accompanied by earwax. The description of the statement about otitis concluded that as many as 70 cat owners, most of them did not understand otitis.
PENGARUH PEMBERIAN EKSTRAK SAMBILOTO (Andrographis Paniculata) TERHADAP GAMBARAN HISTOPATOLOGI GINJAL YANG DIINDUKSI GENTAMISIN PADA TIKUS (Rattus norvegicus) Nugrahani, Anggi Dwi; Nangoi, Lianny; Meles, Dewa Ketut; Widiyatno, Thomas Valentinus; Hamid, Iwan Sahrial; Santoso, Kuncoro Puguh
Journal of Basic Medical Veterinary Vol. 8 No. 1 (2019): Journal of Basic Medical Veterinary, June 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i1.19989

Abstract

This research was aimed to know the effect of leaves extract of sambiloto as a nephroprotector on renal histopathology features induced by gentamicin in white rats (Rattus norvegicus). The effect of leaves extract of sambiloto was tested in 25 rats. Twenty five male white rat were randomly divided into five groups; K- as negative control was given carboxy methyl cellulosa in 15 days, P0 was given carboxyl methyl cellulosa in 15 days, P1 was given leaves extract of sambiloto of 177 mg/kg bw/day/po in 15 days, P2 was given leaves extract of sambiloto of 189 mg/kg bw/day/po in 15 days, P3 was given leaves extract of sambiloto of 239,5 mg/kg bw/day/po in 15 days. On the 15 day of experimental, P0, P1, P2, P3 was given 200 mg/kg bw of gentamicin. Gentamicin solutions on P0, P1, P2 and P3 treated through intramuscular injection. After 24 hours of gentamicin induced, take the rats renal by laparotomy and then making preparations histopathology with staining Haematoxylin-Eosin (HE). Based on the result of Kruskall-Wallis statistical analysis on renal histopathology showed this research that the treatment have significant difference (p < 0,05). The leaves extract of sambiloto treated group (P3) gave the best value on the renal morfology, it gave a good effects in preventing nephrotoxicity in white rats.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP PENURUNAN KADAR MALONDIALDEHID (MDA) PARU MENCIT JANTAN (Mus musculus) YANG DIPAPAR ASAP ROKOK Dewi, Sapna Kurnia; Lokapirnasari, Widya Paramitha; Meles, Dewa Ketut; Mustofa, Imam; Mafruchati, Maslichah; Hamid, Iwan Sahrial
Journal of Basic Medical Veterinary Vol. 8 No. 2 (2019): Journal of Basic Medical Veterinary, December 2019
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v8i2.20416

Abstract

This research aimed to determine the effect of kepok banana peel extract (Musa acuminata) given by per oral in decreasing levels of malondialdehyde (MDA) in lung of male mice (Mus musculus) which was exposed to cigarette smoke. The 24 experimental male mice were divided into 6 groups. The therapy was given for 14 days, all mice were sacrificed and lung MDA level were examined with thiobarbituricacid (TBA) methods. The result of the data analysis test using one-way ANOVA (Analysis of Variance) showed that there were significant differences between the group of animals that exposed to cigarette smoke ( = 840.50± 186.47 nmol / g) and the group of animals that were given with extract of kepok banana peel 56 mg / kg bw  ( = 6471.25 ± 69.25 nmol/g). Besides that, there was an significant difference between the groups of animals that were exposed cigarette smoke ( = 840.50± 186.47 nmol / g) with the group of animals that were given with vitamin C 260 mg/kg bw ( = 555.25± 61.16 nmol / g). It could be concluded that extract of kepok banana peel and vitamin C could decrease MDA levels in lung of male mice which was exposed cigarette smoke. The increased therapy dose gave affect in lowering levels of malondialdehyde in lung.
PENGARUH PEMBERIAN EKSTRAK KULIT PISANG KEPOK (Musa acuminata) TERHADAP GAMBARAN HISTOPATOLOGI BRONKUS DAN VENA PULMONALIS MENCIT (Mus musculus) JANTAN YANG DIPAPAR ASAP ROKOK Jeanita, Winny; Meles, Dewa Ketut; WIdjiati, Widjiati; Hamid, Iwan Sahrial; Luqman, Epy Muhammad; Arimbi, Arimbi
Journal of Basic Medical Veterinary Vol. 9 No. 1 (2020): Journal of Basic Medical Veterinary, June 2020
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/.v9i1.21089

Abstract

This research aimed to attest the effect of Kepok banana (Musa acuminata) peel extract on the histopathology of bronchus and pulmonary veins in male mice (Mus musculus) exposed by cigarette smoke. The 24 experimental male mice were divided into six groups with each treatment having four replicate. All the groups, except K- as a control group, was treated with cigarette smoke for 14 days. After that, K- and K+ was treated with CMC-Na 1%, KO was treated with ascorbic acid 13 mg/kgBW, P1 was treated with Kepok banana peel extract 14 mg/kgBW, P2 was treated with Kepok banana peel extract 28 mg/kgBW, and P3 was treated with Kepok banana peel extract 56 mg/kgBW for 14 days. The data of the thickness of bronchial epithelium and the diameter of pulmonary veins were analyzed using ANOVA (Analysis of Varience) with significance p<0,05 and continued with Post- hoc Tukey. The result of the thickness of bronchial epithelium showed that K+ had the highest thickening (431,83 + 9,71 µm) and significant differences (P<0,05) with all the groups. The result of P3 showed the lowest thickening of bronchial epithelium (170,84 + 2,70 µm). The result of pulmonary veins diameter showed that K+ had the narrowest diameter (525,64 + 16,61 µm) and significant differences (p<0,05) with all the groups. The result of P3 showed the lowest diameter narrowing of pulmonary veins (718,10 + 9,64 µm). The conclussion of this research are Kepok banana peel extract able to decreased the thickness of bronchial epithelium and the narrowing of pulmonary veins diameter in male mice with effective dose 28 mg/ kgBW.