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ANTIBACTERIAL ACTIVITY OF PAPUYU FISH SCALES CHITOSAN (Anabas testudineus) AGAINST Enterococcus faecalis Haliza, Ninda Andrea; Ichrom Nahzi, Muhammad Yanuar; Sitepu, Alexander; Dewi, Nurdiana; Tri Putri, Deby Kania
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22196

Abstract

ABSTRACTBackground: Papuyu fish is widely consumed by Kalimantan people. Papuyu fish scales chitosan has antibacterial potential because it contains amino polysaccharides and enzyme lysozyme which inhibits the growth of root canal bacteria, specifically Enterococcus faecalis. Purpose: The purpose of this experimental is to analyze the antibacterial activity of papuyu fish (Anabas testudineus) scales chitosan against Enterococcus faecalis. Methods: This is a true experimental study with post-test only with a control group design using 15 samples of papuyu fish (Anabas testudineus) scales chitosan grouped into 5 treatment groups and 3 samples repetitions. The treatment groups consisted of papuyu fish (Anabas testudineus) scales chitosan concentrations of 25%, 50%, 75%, 100%, and distilled water (negative control). Absorbance calculation using a UV-Vis Spectrophotometer 722 for minimum inhibitory concentration (MIC) and Enterococcus faecalis colonies are visualized with a colony counter for minimum bactericidal concentration (MBC). Results: The MIC value of papuyu fish (Anabas testudineus) scales chitosan were obtained at 25% concentration and the MBC value were obtained at 100% concentration. The results of One-Way ANOVA test for MIC and Kruskal Wallis for MBC of papuyu fish scales chitosan concentrations of 25%, 50%, 75%, and 100% obtained significant results of p<0,05 and showed a significant difference between treatments. Conclusion: These are differences in the effectiveness of MIC and MBC papuyu fish (Anabas testudineus) scales chitosan concentrations of 25%, 50%, 75%, 100% and distilled water. Papuyu fish scales chitosan 100% concentration is the most effective concentration as an antibacterial against the growth of Enterococcus faecalis.
NANO-HYDROXYAPATITE AND HYDROXYCHAVICOL'S COMBINED EFFECT IN Streptococcus mutans GROWTH Dewi, Nurdiana; Oktiani, Beta Widya; Tri Putri, Deby Kania; Amelia, Aura; Zahra, Aisyah Nur
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22207

Abstract

Background: Early childhood caries is an aggressive form of dental caries that affects children under six years old. The increasing prevalence of ECC caused by Streptococcus mutans highlights the need for effective antimicrobial agents. Objective: To evaluate the antibacterial activity of a combination of nanohydroxyapatite (nHAp) and hydroxychavicol (HC) against S. mutans. Methods: 100 mg/mL nHAp was combined with HC at 0.125, 0.25, 0.5, and 1 mg/mL concentrations. DMSO 5% was used as the negative control. S. mutans ATCC 25175 was cultured in Brain Heart Infusion (BHI) media. Minimum Inhibitory Concentration (MIC) was determined using a UV-Vis spectrophotometer to measure absorbance differences. Minimum Bactericidal Concentration (MBC) was established by plating onto BHI agar and counting the colony count.  Results: The results showed that MIC occurred at a combination of nHAp 100 mg/mL + HC 0.125 mg/mL, while MBC was achieved at nHAp 100 mg/mL + 1 mg/mL HC, where no bacterial colonies were observed. Statistical analysis using ANOVA and Bonferroni post-hoc tests revealed significant differences in both absorbance reduction and colony counts between groups (p < 0.05), confirming the dose-dependent antibacterial efficacy of the nHAp-HC combination. Conclusion: These findings indicate that the synergistic action of nHAp and HC offers a promising strategy for controlling ECC, particularly by targeting the growth of S. mutans. Further, in vivo studies are recommended to explore clinical applicability and long-term safety.
Hubungan Hubungan Pengetahuan Orang Tua terhadap Kualitas Hidup terkait Kesehatan Mulut Anak Down Syndrome Nur Atika; Nurdiana Dewi; Galuh Dwinta Sari
Denta Journal Kedokteran Gigi Vol 18 No 1 (2024): Februari
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v18i1.4

Abstract

Background: Physical, motor, and intellectual retardation experienced by children with Down syndrome leads to low self-care skills and independence so that Down syndrome children depend on their parents in an effort to maintain oral hygiene. Parental knowledge will be one of the predisposing factors that shape the behavior of parents in maintaining children's oral health. Children's oral health can affect functional, psychological, and social aspects that impact the quality of life related to oral health. Objective: To analyze the correlation between the level of parental knowledge about dental and oral health to realize the quality of life in children with Down syndrome at SLB Banjarmasin. Methods: This study is an analytical observational study with a cross-sectional approach. The sampling method used was simple random sampling with 32 respondents from parents of children with Down syndrome at SLB Banjarmasin. The level of parental knowledge and quality of life related to oral health was determined using a modified oral health knowledge questionnaire and a modified Parent-Caregiver Perception Questionnaire (P-CPQ). Results: The results of the Spearman correlation test showed that there was a significant correlation between the level of parental knowledge and the quality of life of children with Down syndrome associated with oral health in SLB Banjarmasin with a significance value of 0.007 (p<0.05). The correlation coefficient value is 0.466. Conclusion: There is a correlation between the level of parental knowledge and the quality of life related to oral health in children with Down syndrome in SLB Banjarmasin City.
Hubungan Pola Asuh Orang Tua terhadap Perilaku Kesehatan Gigi dan Mulut Anak Down Syndrome Syafa Layyina Amalia; Nurdiana Dewi; Riky Hamdani
Denta Journal Kedokteran Gigi Vol 18 No 2 (2024): Agustus
Publisher : Fakultas Kedokteran Gigi Universitas Hang Tuah

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30649/denta.v18i2.4

Abstract

Background: Parental, Down syndrome is a trisomy chromosomal genetic disorder that leads newborn child having more than one chromosome so that could interfere the normal growth of the body due to an excess of protein level. Children with Down syndrome have different oral health problems compared to the general population. They need assistance to maintain their oral health because of mental and physical deficiencies as well as limitations in optimally cleaning their teeth. Objective: To analyze the relationship between parenting styles and oral health behavior of children with Down syndrome in Banjarmasin Special Needs School, Indonesia. Materials and Method: This is an observational analytic study with cross-sectional approach. Sample was taken using simple random sampling technique and a cross-sectional sample formula obtained for 32 respondents. The instrument used a parenting style questionnaire and a behavioral modification questionnaire for children's oral health. Results: Democratic (87.5%) compared switch order parenting styles. It was found that there were more children with Down syndrome in the category of moderate behavior (59%) than children with good behavior (38%) and poor behavior (3%). The results of the Somers'd test data analysis obtained a significance value of 0.034 (p<0.05) and the correlation value obtained was 0.571, which means that the correlation strength is moderate with a positive correlation direction (unidirectional). Conclusion: The more democratic the parents use as parenting style the better the oral health behavior of children with Down syndrome will be.
Co-Authors Ahda Ahda Annisa Ahmad Syaify, Ahmad Akhmad Akhdiannoor Ramadhan Al Supartinah Alfia Fitriani Alfia Fitriani, Alfia Amelia, Aura Amy Nindia Carabelly Ana Azizah Ansari Anita Diana Putri Annisa Rahma Ardi Siswanto Asnawati Asnawati Asnawati Asnawati Aspriyanto, Didit Aulia Azizah Azaria, Nisa Bambang Setiawan Beta Widya Oktiani Debby Saputera, Debby Devi Puspita Handayani Dewi Puspitasari Diana Wibowo Feryra Putri Ayu Suma Feryra Putri Ayu Suma, Feryra Putri Firdaus, I Wayan Arya Krishnawan Gusti Wina Ayu Hazarisa Haliza, Ninda Andrea Hamdani, Riky Haryanto Adi Nugroho Hasvina Sofrullah Hatta, Isnur Hilda Ayu Setyawati Hilda Ayu Setyawati, Hilda Ayu Husnul Khatimah I Wayan Arya Krishnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Ika Kustiyah Oktaviyanti Ika Kusuma Wardani Ivan Arie Wahyudi Kelana, Adhytya Suryo Khatimah, Husnul Lena Rosida Lena Rosida Lia Yulia Budiarti Lisda Hayatie, Lisda Maharani Laillyza Apriasari Maulida Rahmaniah Melinda Hairi Melinda Hairi, Melinda Muhammad Yunanda Anhar Naura Ifthinan Luthfiana Nita Herlina Nita Herlina, Nita Noryunita Rahmah Noryunita Rahmah, Noryunita Nur Atika Nur Tsaniya, Gusti Erysa Nurrahman, Tri Oktiani, Beta Widya Phradina Fili Septishelya Putra, Fedriko Gamaliel Raharja, Suka Dwi Rahmad Arifin Rahmadella, Afifah Renie Kumala Dewi Renita Renita Rahmad Renita Renita Rahmad, Renita Riski Agustin Riski Agustin, Riski Rosihan Adhani, Rosihan Salma Humaira Sari, Galuh Dwinta Setyawardhana, R. Harry Dharmawan Setyawardhana, Raden Harry Dharmawan Sherli Diana Sinaga, Udur Sitepu, Alexander Siti Almira Rahma Siti Bale Sri Rantinah Sonia Dewi Maharani Suka Dwi Raharja Sunjaya Tunggala Sunjaya Tunggala, Sunjaya Syafa Layyina Amalia Tri Putri, Deby Kania Utami, Juliyatin Putri Wahyuni A Wahyuni A, Wahyuni Yasmina Aulia Yusfarani, Mirza Fitria Yusrinie Wasiaturrahmah Zahra, Aisyah Nur Zainatun Nadhira