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All Journal FIKkeS Majalah Kedokteran Gigi Indonesia Dentino: Jurnal Kedokteran Gigi Dental Journal (Majalah Kedokteran Gigi) Padjadjaran Journal of Dentistry Journal of Dentomaxillofacial Science E-Prodenta Journal of Dentistry Dentin Jurnal Al-Hakim : Jurnal Ilmiah Mahasiswa Studi Syariah, Hukum dan Filantropi
Dewi Nurdiana
Department Of Oral Biology, University Of Lambung Mangkurat Banjarmasin, Indonesia
Religion Biochemistry, Genetics & Molecular Biology Dentistry Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Public Health Social Sciences

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ANTIBACTERIAL ACTIVITY OF PAPUYU FISH SCALES CHITOSAN (Anabas testudineus) AGAINST Enterococcus faecalis Haliza, Ninda Andrea; Ichrom Nahzi, Muhammad Yanuar; Sitepu, Alexander; Dewi, Nurdiana; Tri Putri, Deby Kania
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22196

Abstract

ABSTRACTBackground: Papuyu fish is widely consumed by Kalimantan people. Papuyu fish scales chitosan has antibacterial potential because it contains amino polysaccharides and enzyme lysozyme which inhibits the growth of root canal bacteria, specifically Enterococcus faecalis. Purpose: The purpose of this experimental is to analyze the antibacterial activity of papuyu fish (Anabas testudineus) scales chitosan against Enterococcus faecalis. Methods: This is a true experimental study with post-test only with a control group design using 15 samples of papuyu fish (Anabas testudineus) scales chitosan grouped into 5 treatment groups and 3 samples repetitions. The treatment groups consisted of papuyu fish (Anabas testudineus) scales chitosan concentrations of 25%, 50%, 75%, 100%, and distilled water (negative control). Absorbance calculation using a UV-Vis Spectrophotometer 722 for minimum inhibitory concentration (MIC) and Enterococcus faecalis colonies are visualized with a colony counter for minimum bactericidal concentration (MBC). Results: The MIC value of papuyu fish (Anabas testudineus) scales chitosan were obtained at 25% concentration and the MBC value were obtained at 100% concentration. The results of One-Way ANOVA test for MIC and Kruskal Wallis for MBC of papuyu fish scales chitosan concentrations of 25%, 50%, 75%, and 100% obtained significant results of p<0,05 and showed a significant difference between treatments. Conclusion: These are differences in the effectiveness of MIC and MBC papuyu fish (Anabas testudineus) scales chitosan concentrations of 25%, 50%, 75%, 100% and distilled water. Papuyu fish scales chitosan 100% concentration is the most effective concentration as an antibacterial against the growth of Enterococcus faecalis.
NANO-HYDROXYAPATITE AND HYDROXYCHAVICOL'S COMBINED EFFECT IN Streptococcus mutans GROWTH Dewi, Nurdiana; Oktiani, Beta Widya; Tri Putri, Deby Kania; Amelia, Aura; Zahra, Aisyah Nur
Dentino: Jurnal Kedokteran Gigi Vol 10, No 1 (2025)
Publisher : FKG ULM

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20527/dentino.v10i1.22207

Abstract

Background: Early childhood caries is an aggressive form of dental caries that affects children under six years old. The increasing prevalence of ECC caused by Streptococcus mutans highlights the need for effective antimicrobial agents. Objective: To evaluate the antibacterial activity of a combination of nanohydroxyapatite (nHAp) and hydroxychavicol (HC) against S. mutans. Methods: 100 mg/mL nHAp was combined with HC at 0.125, 0.25, 0.5, and 1 mg/mL concentrations. DMSO 5% was used as the negative control. S. mutans ATCC 25175 was cultured in Brain Heart Infusion (BHI) media. Minimum Inhibitory Concentration (MIC) was determined using a UV-Vis spectrophotometer to measure absorbance differences. Minimum Bactericidal Concentration (MBC) was established by plating onto BHI agar and counting the colony count.  Results: The results showed that MIC occurred at a combination of nHAp 100 mg/mL + HC 0.125 mg/mL, while MBC was achieved at nHAp 100 mg/mL + 1 mg/mL HC, where no bacterial colonies were observed. Statistical analysis using ANOVA and Bonferroni post-hoc tests revealed significant differences in both absorbance reduction and colony counts between groups (p < 0.05), confirming the dose-dependent antibacterial efficacy of the nHAp-HC combination. Conclusion: These findings indicate that the synergistic action of nHAp and HC offers a promising strategy for controlling ECC, particularly by targeting the growth of S. mutans. Further, in vivo studies are recommended to explore clinical applicability and long-term safety.
Co-Authors Ahda Ahda Annisa Ahmad Syaify, Ahmad Akhmad Akhdiannoor Ramadhan Al Supartinah Alfia Fitriani Alfia Fitriani, Alfia Amelia, Aura Amy Nindia Carabelly Ana Azizah Ansari Anita Diana Putri Annisa Rahma Ardi Siswanto Asnawati Asnawati Asnawati Asnawati Aspriyanto, Didit Aulia Azizah Azaria, Nisa Bambang Setiawan Beta Widya Oktiani Debby Saputera, Debby Devi Puspita Handayani Dewi Puspitasari Diana Wibowo Feryra Putri Ayu Suma Feryra Putri Ayu Suma, Feryra Putri Firdaus, I Wayan Arya Krishnawan Gusti Wina Ayu Hazarisa Haliza, Ninda Andrea Hamdani, Riky Haryanto Adi Nugroho Hasvina Sofrullah Hatta, Isnur Hilda Ayu Setyawati Hilda Ayu Setyawati, Hilda Ayu Husnul Khatimah I Wayan Arya Krishnawan Firdaus Ichrom Nahzi, Muhammad Yanuar Ika Kustiyah Oktaviyanti Ika Kusuma Wardani Ivan Arie Wahyudi Kelana, Adhytya Suryo Khatimah, Husnul Lena Rosida Lena Rosida Lia Yulia Budiarti Lia Yulia Budiarti Lisda Hayatie, Lisda Maharani Laillyza Apriasari Maulida Rahmaniah Melinda Hairi Melinda Hairi, Melinda Muhammad Yunanda Anhar Naura Ifthinan Luthfiana Nita Herlina Nita Herlina, Nita Noryunita Rahmah Noryunita Rahmah, Noryunita Nur Tsaniya, Gusti Erysa Nurrahman, Tri Oktiani, Beta Widya Phradina Fili Septishelya Putra, Fedriko Gamaliel Raharja, Suka Dwi Rahmad Arifin Rahmadella, Afifah Renie Kumala Dewi Renita Renita Rahmad Renita Renita Rahmad, Renita Riski Agustin Riski Agustin, Riski Rosihan Adhani, Rosihan Salma Humaira Sari, Galuh Dwinta Setyawardhana, R. Harry Dharmawan Setyawardhana, Raden Harry Dharmawan Sherli Diana Sinaga, Udur Sitepu, Alexander Siti Almira Rahma Siti Bale Sri Rantinah Sonia Dewi Maharani Suka Dwi Raharja Sunjaya Tunggala Sunjaya Tunggala, Sunjaya Tri Putri, Deby Kania Utami, Juliyatin Putri Wahyuni A Wahyuni A, Wahyuni Yasmina Aulia Yusfarani, Mirza Fitria Yusrinie Wasiaturrahmah Zahra, Aisyah Nur Zainatun Nadhira