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All Journal Buletin Udayana Mengabdi Jurnal Veteriner Indonesia Medicus Veterinus Microbiology Indonesia Media Kedokteran Hewan Jurnal Medik Veteriner Journal of Applied Veterinary Science and Technology Jurnal Kedokteran Hewan Journal of Veterinary and Animal Sciences Buletin Veteriner Udayana
Ida Bagus Oka Winaya
Faculty Of Veterinary Medicine, Udayana University
Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology Medicine & Pharmacology Public Health Veterinary

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Journal : Jurnal Veteriner

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Karakteristik Molekuler Virus Avian Orthoavulavirus 1 Genotipe VII yang Diisolasi dari Tabanan Bali (MOLECULAR CHARACTERISTIC OF AVIAN ORTHOAVULAVIRUS 1 GENOTYPE VII ISOLATED FROM TABANAN BALI) Anak Agung Ayu Mirah Adi; I Nyoman Mantik Astawa; I Nengah Wandia; I Gusti Agung Arta Putra; Ida Bagus Oka Winaya; Anak Agung Keswari Krisnandika; Anak Agung Oka Wijaya
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (179.964 KB) | DOI: 10.19087/jveteriner.2019.20.4.593

Abstract

Newcastle disease (ND) is a very harmful avian disease, endemic in Indonesia and various parts of the world. The causative agent is ND virus or Avian orthoavulavirus 1 (AOAV-1). This virus is an RNA virus with wide genetic variation. Based on the genome length, it can be classified into AOAV-1 Class I and II. Class I are generally avirulent whereas Class II are consist of both virulent and avirulent viruses, currently there are 18 genotypes of the class II. To find out the molecular characteristics of AOAV-1 currently circulating in the field, isolation and identification of viruses from laying hens that was suspected ND from Tabanan Bali in 2017, was performed. The isolated viruses hereafter named as Tabanan1/ARP / 2017. A one-step RT-PCR reaction was carried out to amplify NP, F and HN gene fragments from the virus using three specific pairs of AOAV-1 primers. The obtained nucleotide sequences are then used in phylogenetic analysis. For phylogenetic analysis several strains of AOAV-1 from class II representing genotype I-VII as well as one strain from Class I were accessed from GenBank. From the analysis of the F gene nucleotide sequences, it was found that Tabanan 1 / ARP / 2017 is a genotype VII virus with an amino acid sequence at the F protein cleavage site is 112 R-R-Q-K-R-F117, a typical virulent strain. Phylogenetic analysis using nucleotide sequences NP and HN genes also positioned this isolate in genotype VII. At the nucleotide level, genetic distance with virulent isolates that was isolated in 2007 and 2010 were 8.26% and 1.08% while at the amino acid level were 5.26% and 0.64%. There were found mutations in amino acids at positions 107 and 108 of F protein.
Identifikasi Ekspresi Glikokonjugat pada Jaringan Limfonodus Sapi Bali yang Terinfeksi Virus Penyakit Jembrana Secara Eksperimental Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi
Jurnal Veteriner Vol 10 No 1 (2009)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of this study is to compare glycoconjugate expression in the lymphonodes between bali cattleexperimentally infected with jembrana virus disease and non infected cattle. The biotinylated lectinsused in this study were Concavalia ensiformis (ConA), Arachis hypogaea (WGA), Triticum vulgaris (PNA),Ulex europeaus (UEA-1), and Sambucus nigra (SNA), respectively. The expression of lectin bindingglycoconjugate exhibit differences between the lymphonodes of infected and non infected cattle. Con A,WGA, and SNA were found be bound to lymphocytes whereas in infected cattle it was less intensive,repectively. More over, PNA and UEA were found strongly bound to the either lymphocytes of eitherinfected and non infected cattle.
Gambaran Histopatologi Selaput Lendir Kantung Empedu Sapi Bali yang Terinfeksi Cacing Fasciola gigantica (HISTOPATOLOGICAL ILLUSTRATION OF GALL BLADDER MUCOUS MEMBRANE INFASCIOLA GIGANTICA INFECTED BALI CATTLE) I Made Kardena; Ida Bagus Oka Winaya; Elyda .; I Dewa Made Adhiwitana; AAA Mirah Adi; I Ketut Berata
Jurnal Veteriner Vol 17 No 1 (2016)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

This study aims were to describe lesions and their intensities of histopathological changes in gallbladder mucosa of bali cattle that infected with Fasciola gigantica. As many as 42 gall bladers of balicattle were used in this studi, i.e: 35 samples were infected and sefen were non-infected with F.gigantica.The samples were randomly gathered from Pesanggaran slaughter house in Denpasar. The gall bladderswere evaluated macroscopically and prepared for microscopically examination using haematoxylin andeosin staining.The result showed that there were histopathological changes observed in the mucosa of thegall bladder:necrosis cells of gall bladder with intencity 100%, hemorrhage 68.5%, hyperplasia serousgland and mucous hypertrophy gland 100%, collagen in filtration 100%, fibroblast proliferation 91.4%,and infiltration of inflammation cells, 74.2%. Histopathological changes in gallbladders mucous of balicattle infected F.gigantica experiencing severe lesons with frequency percentage above 50%.
Gambaran Histopatologi Kulit Anjing Penderita Dermatitis (HISTOPATHOLOGICAL FEATURES OF DOG’S SKIN WITH DERMATITIS) Komang Andika Purnama; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Gusti Made Krisna Erawan; I Made Kardena; I Nyoman Suartha
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (258.116 KB) | DOI: 10.19087/jveteriner.2019.20.4.486

Abstract

Complex dermatitis is a disorder of the skin caused by the combination of several agents such as fungi, ectoparasites, bacteria, and metabolic diseases with combined clinical symptoms of primary lesions and secondary lesions. This study aims to determine the histopathology of dog skin with complex dermatitis. There were 15 samples of dogs with complex dermatitis, skin biopsy and histopathology. The results showed that the incidence of complex dermatitis in young dogs was 60%, long-haired 67%, male sex 73%, and domestic race 73%. Macroscopic examination found a change in hyperkeratosis. Microscopically observed that infiltration of polymorphonuclear cells, monomorphonuclear cells and macrofag from moderate to severe in the dermis. In hair follicles and epidermis occured akantosis, and keratin proliferation. There were segment fragments from infectious agents such as Demodex canis, Sarcoptes scabiei, and yeast from Malasezia sp. Other changes were observed in the presence of hydrophic degeneration, necrosis, and ulcers. Dogs suffering from complex dermatitis have chronic infections with more than one infectious agent. Disabilities based on histopathological observation of dogs with complex dermatitis with moderate severity of 33%, and severe 67%.
Studi Kasus: Ascariosis Disertai Migrasi Larva pada Hati dan Paru-paru Babi Landrace (CASE STUDY: ASCARIOSIS ACCOMPANIED BY MIGRATION OF LARVAE IN LIVER AND LUNGS OF LANDRACE PIG) I Ketur Berata; Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Made Kardena; Ida Bagus Windia Adnyana
Jurnal Veteriner Vol 20 No 4 (2019)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (401.797 KB) | DOI: 10.19087/jveteriner.2019.20.4.603

Abstract

Cases of ascariosis due to infection with Ascaris suum are still prevalent in the world, including Indonesia. Apart from causing economic losses, ascariosis can also threaten human health because it is zoonotic. Case studies of ascariosis with typical white spot lesions in the landrace pig liver is described in this paper. Pigs aged 60 days old, sick for 10 days, thin and coughing gathered from the village of Suwung, Denpasar City. After necropsy and post mortem examination were done, tens of A. suum worms were found in the intestine and white spot lesions observed on the livers. Tissues of small intestine, large intestine, liver, lungs, kidney, spleen, urinary vesicles and brain were taken and put into 10% of formalin neutral buffer before they then processed in making histopathological preparations. Histopathological preparation was carried out using paraffin block method and hematoxylin eosin (HE) staining technique. The results of histopathological examination of the small intestine, large intestine, liver and lungs were found to be infiltrated by eosinophil cells. Eosinophil cells infiltration indicates that there are parasites A. suum or the larvae migrant in the tissues. Some of the tissues in livers were also found to have fibrosis, which is suspected that the infection has been chronic. It can be concluded that cases of the landrace pigs were chronically infected by A.suum and accompanied by migration of the larvae to the livers and lungs. More attention is needed to prevent the increase of ascariosis in pigs in order to minimize economic losses and transmission to humans.
Deteksi Virus Classical Swine Fever di Bali dengan RT-PCR I Wayan Wirata; Ida Ayu Sri Chandra Dewi; I Gusti Ngurah Narendra Putra1,; Ida Bagus Oka Winaya; Ida Bagus Kade Suardana3,; Tri Komala Sari; I Nyoman Suartha; I Gusti Ngurah Kade Mahardika
Jurnal Veteriner Vol 11 No 3 (2010)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Classical Swine Fever (CSF) virus has been confirmed for the first time in pig in Bali. The object of thisstudy was suspected CSF cases diagnosed at the diagnostic laboratory assistantship of the Faculty ofVeterinary Medicine, Udayana University, in 2007-2008. Total number of cases was 12. Case recordsincluded the signalment of case (breed, age, body weight, and the origin of respective case), clinical signs,post-mortem lesions, and histological pictures. CSF virus was confirmed using the standardized reversetranscriptase-polymerase chain reaction (RT-PCR) for CSF from European Union. One RT-PCR productwas sequenced. CSF virus was confirmed in seven out of 12 cases (58%). The cDNA sequence wasconfirmed to be specific of CSF E2 protein coding region with 98% homology to one isolate from China thatwas available in GeneBank. Further works are recommended to elucidate the sensitivity of RT-PCR, toclarify some differential diagnose, and to find out the genetic variation of CSF virus in Bali.Key words: classical swine fever virus, Bali, RT-PCR
Pengaruh Pemberian Ekstrak Akar Alang-Alang terhadap Hati Mencit yang Menderita Gangguan Patologi (THE INFLUENCE OF THE ROOT OF SEDGE GRASS EXTRACTION ON LIVER OF MICES THAT TO SUFFER A PATHOLOGICAL DAMAGED) I Ketut Berata; Ida Bagus Oka Winaya
Jurnal Veteriner Vol 4 No 1 (2003)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

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Gambaran Histopatologi Hati dan Ginjal Anjing Terduga Leptospirosis di Kota Denpasar (HISTOPATHOLOGICAL CHANGES OF LIVER AND KIDNEY DOG SUSPECTED CANINE LEPTOSPIROSIS IN DENPASAR CITY) Ida Bagus Oka Winaya; I Ketut Berata; I Made Kardena; Anak Agung Ayu Mirah Adi; Aida Louise Tenden Rompis
Jurnal Veteriner Vol 19 No 2 (2018)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (155.475 KB) | DOI: 10.19087/jveteriner.2018.19.2.298

Abstract

This study aims to determine the pathological changes in dog suspected of being infected by Leptospira spp. This research used a retrospective study design. A total of 210 canines sample were examined at Faculty of Veterinary Medicine The Udayana of University between January 2008 to January 2010. Of the 210 specimens, five canines with clinical sign anorexia, fever, vomiting, polyurea, dyspnea and only one are noted icteric in the sclera. Macroscopically : the anemic to petechial haemorrhagis was find in pulmo, icterus in liver with gall blader distention, kidney swollen with black color in hillus, spleen rather swollen with black color on end and mild hemorrhagis on intentines. The infected organ is inserted into a pot filled with neutral buffer formalin 10%. Then stained with haematoxyllin-eosin for the microscopic preparation. Microscopic examimination revealed the presence of spiral bacterial cells on the necrotic liver and kidneys tissue. Infiltration neutrophils and macrophages was also find in pulmonary and spleen tissue. Besided that intertubuler edematous were also observed under microscopic examination. It can be concluded changes associated with Leptospira spp infection can be found in liver and kidney tissue with changes such as necrosis, proliferation of kuffer cells and intertubular edematous.
Pneumonia Verminosa pada Kucing Lokal yang Terinfeksi oleh Aelurotsrongylus sp (VERMINOUS PNEUMONIA IN DOMESTIC CAT INFECTED BY AELUROSTRONGYLUS SP) Ida Bagus Oka Winaya; I Ketut Berata; I Made Kardena; Ida Bagus Made Oka
Jurnal Veteriner Vol 13 No 4 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The aim of the study was to evaluate the pulmo pathological changes of domestic cat infected byAelurostrongylus sp. A total of 15 cats were examined at Faculty of Veterinery Medicine, Udayana Universityduring 2010. Ten out of 15 cats showed sneezing, whereas the remains showed serous rhinitis and sneezing.Macroscopic and microscopic changes were observed mainly on pulmo samples. Hyperemias on caudalislobes and pleura effusion were found in the pulmo. The pulmo tissue was fixed on 10 % neutral bufferformalin and stained with haematoxilin-eosin (HE) for histopathological examination. Aelurostrongylus spwas present in the alveoli lumen of the lung samples. Meanwhile, the alveoli septa of the lung wereobserved thicker and infiltrated with neutrophils, plasma exudates and erythrocytes. Pleural effusion wasmainly consisted of eosinophilic substances. It is concluded that verminous pneumonia in domestic catinfected with Aelurostrongylus sp was an acute infection.
Perubahan Patologi Sistem Hepatobiliari Sapi Bali Terinfeksi Fasciola gigantica Ida Bagus Oka Winaya; Anak Agung Ayu Mirah Adi; I Ketut Berata; I Made Kardena; Ida Bagus Windia Adnyana; Ida Bagus Kade Suardana
Jurnal Veteriner Vol 21 No 4 (2020)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (160.416 KB) | DOI: 10.19087/jveteriner.2020.21.4.654

Abstract

Liver fluke infections is an important parasitic disease that common attack cattle, buffalo and others ruminant. The disease in the tropics is caused by Fasciola gigantica trematodes. Fasciolosis found in Bali cattle besides causing high economic loss also has the opportunity infect humans. This study aims to determine the pathology changes of Bali cattle hepatobiliary system infected by F. gigantica. In January2019 were examined a hundred of liver tissue Bali cattle. Based on the sex as many as 75 bali cattle are cows and 25 are bulls. On macroscopic examination was found fifteen bali cattles in gallbladder containing of F. gigantica. In positive Fasciolosis the surface of liver look uneven with enlargement of lymph nodes. The tissue of Bali cattle liver infected with F. gigantica is cut into 1x1x1 cm and put in a pot that has been filled with 10% neutral buffered formaline. The fixed tissue then processed in a tissue processor and stained with hematoxylin-eosin (HE). Microscopic examination was found bile ducts proliferation and blocked of the lumen accompanied by cholangio cirrhosis. Found longitudinal pieces of Fasciola giganticaliver fluke surrounded by fibrous connective tissue and multifocal coagulative necrosis with fibroblast proliferation. In some location the infiltration of neutrophil cells around the bile ducts can also be seen. Congestion, bleeding, and neutrophil infiltration are also seen in areas of necrosis. In the bile duct wall bleeding, adenomatus hyperplasia, infiltration of mononuclear cells with mild intensity and foci ofcalcification are found. It can be concluded that there was a change with severe intensity accompanied by chronic inflammation in the hepatobiliary system of Bali cattle with Fasciolosis.
Co-Authors Achoiro Wati Rasid Aida Lousie Tenden Rompis Alya Nita Shena Gayanti Anak Agung Ayu Mirah Adi Anak Agung Ayu Mirah Adi Anak Agung Bagus Bramardipa Anak Agung Keswari Krisnandika Anak Agung Oka Wijaya Anak Agung Sagung Kendran Aras, Fatmawati Astuti, Ni Made Widy Matalia Baiq Renny Kamaliani BERATA BERATA Bire, Ienoliski Rohi Brigietta Vincencia Simanihuruk Cahyani, Ni Kadek Devi Desak Wiga Puspita Dewi Dewa Odiec Purnawan Djara, Devita Vanessa Sukmawati DWI SURYANTO Dzikri Nurma'rifah Takariyanti Elyda . Fatmawati Aras Fauzia Hadista Anjani Febrianty, Ni Made Dhea Guru, Yohana Cendyka Kartika Dewi Gusti Agung Ayu Putu Adriyati, Gusti Agung Ayu Gusti Ayu Yuniati Kencana Gusti Ayu Yuniati Kencana Hapsari Mahatmi Husain, Anugrah Fitria I Dewa Made Adhiwitana I Gede Made Andy Pratama I Gede Yoga Sukya Sumantara I Gusti Agung Arta Putra I Gusti Agung Gede Putra Pemayun I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Ketut Suarjana I Gusti Made Krisna Erawan I Gusti Ngurah Kade Mahardika I Gusti Ngurah Kade Mahardika I Gusti Ngurah Narendra Putra1, I Kadek Parnayasa I Ketur Berata I Ketut Berata I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Eli Supartika I Ketut Suarjana I Made Damriyasa I Made Dwinata I Made Kardena I Made Merdana I Made Merdana I Made Merdana I Made Sukada i Nengah Wandia I Nyoman Mantik Astawa I NYOMAN MANTIK ASTAWA I Nyoman Suarsana I Nyoman Suartha I Putu Arya Adikara I Putu Cahyadi Putra I Putu Cahyadi Putra, I Putu Cahyadi I Wayan Gorda I Wayan Gorda I Wayan Sudarmayasa I Wayan Sudira I Wayan Wirata I.K. Berata IB. KADE SUARDANA Ida Ayu Adhistania Pidada Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Sri Chandra Dewi Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Kade Suardana Ida Bagus Komang Ardana Ida Bagus Made Oka Ida Bagus Ngurah Swacita Ida Bagus Windia Adnyana Iwan Harjono Utama Iwan Haryono Utama Kadek Karang Agustina Komang Andika Purnama Komang, Nadia Eprillia Sary Darma Ni Kurnia, Ida Ayu Gintan Aristi Luh Made Sudimartini LUH PUTU AGUSTINI Luh Putu Ratna Suhita Lutviana, An'nisafitri M. Farhan Al Ma'arif Marissa Divia Dayanti Marissa Divia Dayanti Marson, Fransiska Gratia Sonita MAS DJOKO RUDYANTO Mia Monica Ni Kadek Eka Widiadnyani Ni Kadek Nining Laksmi Dewi Ni Kadek Puspa Dewi Ni Komang Wijayani Ni Luh Aricahyani Ni Luh Eka Setiasih Ni Luh Putu Ratna Suhita Ni Nyoman Werdi Susari Ni Putu Trisna Asih NINING HARTANINGSIH Nur Baiti Palagan Senopati Sewoyo Palagan Senopati Sewoyo Pinem, Nuh Lasjuardi Purnama, Komang Andika Purnasari, Maria Elisabeth Putra, I Putu Werdikta Jayantika Putu Agus Trisna Kusuma Antara Putu Henrywaesa Sudipa Putu Intan Kusuma Wardani Rahmat Grahadi RAHMI MUSTABA Ryan Helmi Habibi Samsuri Samsuri Sartika, Nola Alfien Sartika, Nola Alfieni Sewoyo, Palagan Senopati Shafira Laili Aulia Shafira Laili Aulia Sri Kayati Widyastuti Sugiyarto - Tampubolon, Yandri Putra Lumatas Theresia Ene Tjok Gde Oka Pemayun Tjokorda Sari Nindhia Tono PG, I Ketut TRI KOMALA SARI Tyas Pandieka Yoga Velia Chyntia Victoria Wijaya, Anak Agung Gede Oka Wira, Amar Yasa, Gede Sastra Darma Yeni Ratna Sari Yeyen Agustianingsi Yolla Noviolita Yoshihiro Hayashi YUNNY DAMAYANTI Yustisia, Anggia