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Journal : Bionature

Pengaruh Pemberian Serbuk Daun Sirsak terhadap Sitophilus oryzae pada Beras Selama Penyimpanan Halifah Pagarra
Bionature Vol. 9 No. 2 (2008): 2008
Publisher : Jurusan Biologi Universitas Negeri Makassar

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Abstract

This study aims to determine the influence of leaf of Anona muricata L. powder against Sitophilus oryzae in rice during storage. This research is experimental research that uses experimental design with a basic design pattern randomize complete design. This study consists of four treatment groups (K0, K1, K2, and K3). K0 = control or no treatment; K1 = 10% (90 grams + 10 grams of rice powder leaves of Anona muricata L.); K2 = 20% (80 grams + 20 grams of rice powder leaves sirsak; K3 = 30% (70 grams + 30 grams of rice powder leaves Anona muricata L). Parameters measured the number of insects which declined during the 1 month storage. Data obtained in deskritif and analyzed statistically with inferensial test Fα = 0.05 followed by the LSD α = 0.05. Results of research indicate that the results of research show that the leaves of Anona muricata L powder. affect Sitophilus oryzae in rice during storage. Concentration of the most influential of Sitophilus oryzae in rice during storage is K3 (concentration 30%), the number of insects that are rejected 10 insects.
Optimization of Pectin Extraction from Pomelo Peels (Citrus maxima) using Response Surface Methodology Pagarra, Halifah; Hartati; Rachmawaty, Rachmawaty; Sahribulan, Sahribulan
Bionature Vol. 25 No. 1 (2024): April-Juni 2024
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v25i1.2340

Abstract

Pectin is an essential polysaccharide that is usually found in the skin of various fruits and vegetables. It acts as a gelling agent and stabilizer in many food and pharmaceutical industries. This study focuses on the potential of grapefruit peel as a source of pectin. Pectin is isolated from hot grapefruit peel powder (albedo). The aim of this study was to extract pectin from grapefruit peel (albedo), and central composite design (CCD) together with the numerical optimization method of response surface methodology (RSM) was used to optimize the processing parameters, including temperature (60, 80 and 100 ° C), pH (1.5, 2.0 and 2.5 ) and time (60, 90 and 120 min) The findings show that the studied parameters have a major impact on the pectin yield. The resulting second-order polynomial model thoroughly explains the relationship between input and output variables and how the data is varied. The highest pectin yield was 28.45% at optimal conditions achieved: temperature: 100° C; pH: 1.5; and extraction time: 120 minutes. Temperature, pH, and extraction time have their own large impact on pectin yield which has a significant effect.
Antibacterial Activity Test of Matoa (Pometia pinnata) Leaves Ethanol Extract Against Salmonella typhi and Bacillus subtilis Pagarra, Halifah; Taiyeb , Mushawwir; Junda , Muhammad; Musawira; Muhammad Naufal Syaiful Haq; Handayani , Resti
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7234

Abstract

This study aims to determine the antimicrobial activity of ethanol extract of matoa leaves (Pometia pinnata) against Salmonella typhi and Bacillus subtilis bacteria. This study began with preparing raw materials in the form of matoa leaves and then extracting the matoa leaves using the maceration method with 70% solvent. After that, the antimicrobial activity test was carried out using the disc diffusion method with four concentration variations, namely 5%, 7.5%, 10%, and 12.5% with a positive control in the form of 50 ppm amoxicillin and a negative control using distilled water, each treatment using three replications. The results in the form of inhibition zones formed were measured using calipers. The data obtained were then analyzed using SPSS 30 with the ANOVA test and further tests in the form of tests. The test results showed that 70% ethanol extract of matoa leaves had an effect on inhibiting the growth of Salmonella typhi and Bacillus subtilis bacteria. The largest inhibition zone in Salmonella typhi was found at a concentration of 7.5% with a diameter of 16.87 mm, while in Bacillus subtilis it was found at a concentration of 12.5% with a diameter of 12.22 mm. Keywords: Pometia pinnata, Antimicrobial activity, Salmonella typhi, Bacillus subtilis
The Effect of Matoa (Pometia Pinnata) Leaf Ethanol Extract on Reducing Blood Sugar Level in Male Mice (Mus Musculus) Induced by Alloxan Pagarra, Halifah; Salsabila, Shopia; Muis, Abd; Naufal Syaiful Haq, Muhammad
Bionature Vol. 26 No. 1 (2025): April-Juni 2025
Publisher : Jurusan Biologi Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/bionature.v26i1.7361

Abstract

This research aims to determine the total phenolic content and antioxidant activity of the ethanol extract from Matoa (Pometia pinnata) leaves as well as its effect on blood sugar levels in male mice (Mus musculus) induced with alloxan. To determine total phenolic content using the Folin-Ciocalteu method, and the DPPH method to measure antioxidant activity. The effect of Matoa leaves on blood sugar reduction in mice used a completely randomized design (CRD) with 15 mice divided into 5 treatments with 3 replications: namely P1 (Extract 125 mg/kg BW), P2 (Extract 250 mg/kg BW), P3 (Extract 500 mg/kg BW), K+ (Glibenclamide), and K- (Na-CMC 0.5%). Extraction used the maceration method with 70% ethanol solvent. The results of the total phenolic test showed the presence of phenolics with a value of 26.24872 mg GAE/g. The measurement of antioxidant activity yielded an IC50 value of 1.961 ppm. The results of testing the blood sugar levels showed that there was an effect of giving ethanol extract of Matoa (Pometia pinnata) leaves on reducing blood sugar levels in male mice (Mus musculus). Giving of ethanol extract of Matoa leaves at a dose of 250 mg/kg BW is effective in reducing blood sugar levels. So it can be concluded that the ethanol extract of Matoa leaves has an effect on reducing blood sugar levels in male mice