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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia VALENSI Biospecies Jurnal MIPA AL KAUNIYAH Buletin Plasma Nutfah Transformasi: Jurnal Pengabdian Masyarakat Unnes Science Education Journal Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Jurnal Mikologi Indonesia Bioma : Jurnal Biologi Indonesia Biosaintifika: Journal of Biology & Biology Education Acta Biochimica Indonesiana Health Information : Jurnal Penelitian Jurnal Riset Sains dan Kimia Terapan Journal of Microbial Systematics and Biotechnology JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Pengabdian kepada Masyarakat Nusantara Aptekmas : Jurnal Pengabdian Kepada Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Buitenzorg: Journal of Tropical Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia

Dalia Sukmawati

Universitas Negeri Jakarta

Author-ID : 3454724

Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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Histomorfometri Duodenum Mencit (Mus musculus) yang Diinfeksi Telur Infektif Hymenolepis nana dan diberi Ekstrak Daun Kelor (Moringa oleifera Lam.) Atin Supiyani; Sekar Liyundzira; Daniel Ramadhan; Dalia Sukmawati
Jurnal MIPA Vol. 12 No. 2 (2023): Artikel
Publisher : Universitas Sam Ratulangi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35799/jm.v12i2.45964

Cacing cestoda Hymenolepis nana merupakan cacing parasit intestinal yang bersifat zoonosis. Infeksi dari cacing H.nana berdampak buruk pada saluran pencernaan, terutama pada duodenum host. Tujuan dari penelitian ini adalah untuk menguji pengaruh dari pemberian ekstrak daun kelor terhadap struktur duodenum mencit yang diinfeksi H.nana. Sebanyak 27 ekor mencit dibagi dalam 3 kelompok yaitu aquades (kontrol negatif), albendazole (kontrol positif), dan ekstrak daun kelor 500 ppm. Dosis letal 100 diperoleh dari uji in vitro ekstrak daun kelor pada telur dan larva cacing H.nana pada masa inkubasi 24, 48 dan 72 jam. Setiap mencit diinfeksi 40 butir telur H.nana secara oral. Ekstrak daun kelor diberikan selama 21 hari setelah infeksi. Histomorfometri struktur duodenum dengan mengukur tinggi vili, tebal mukosa, sub-mukosa, tunika muskularis, dan serosa pada 10 vili. Hasil penelitian diperoleh dosis letal 100-24 jam ekstrak daun kelor terhadap telur H.nana sebesar 397 ppm. Tinggi vili, tebal lapis mukosa, sub-mukosa, muskularis dan serosa mencit yang diberi ekstrak daun kelor berbeda signifikan (Sig<0.05). Pemberian ekstrak daun kelor dapat mempengaruhi struktur duodenum mencit yang terinfeksi cacing Hymenolepis nana. The cestode worm Hymenolepis nana is a zoonotic intestinal parasitic worm. Infection from H. nana worms adversely affects the gastrointestinal tract, especially on the host duodenum. The purpose of this study was to determine the effect of Moringa leaf extract on the structure of the duodenum of mice infected with H. nana. A total of 27 mice were divided into 3 groups, namely aquades (negative control), albendazole (positive control), and Moringa leaf extract of 500 ppm. A lethal dose of 100 was obtained from in vitro tests of Moringa leaf extract on eggs and larvae of H. nana worms during the incubation period of 24, 48 and 72 hours. Each mice is infected with 40 H. nana eggs orally. Moringa leaf extract is administered for 21 days after infection. Histomorphometry of duodenal structures by measuring villi height, mucosal thickness, sub-mucosa, muscular tunica, and serous on 10 villi. The results of the study obtained a lethal dose of 100-24 hours of Moringa leaf extract against H. nana eggs of 397 ppm. Tall villi, thick layer mucosa, sub-mucosa, muscular and serous mice given Moringa leaf extract differed significantly (Sig<0.05). Administration of Moringa leaf extract can affect the structure of the duodenum of mice infected with Hymenolepis nana

Peningkatan kesadaran dan pengetahuan kesehatan reproduksi remaja putri di SMP Islam At-Tawwabiin Cilangkap Tapos Depok Atin Supiyani; Dalia Sukmawati
Transformasi: Jurnal Pengabdian Masyarakat Vol. 19 No. 1 (2023): Transformasi Juni
Publisher : LP2M Universitas Islam Negeri Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20414/transformasi.v19i1.5790

[Bahasa]: Kesehatan reproduksi pada remaja putri sangat dipengaruhi oleh tingkat pengetahuan terkait organ dan sistem reproduksi. Usia remaja merupakan usia kritis dimana remaja putri pertama kali mengalami fase pubertas yang ditandai dengan dimulainya siklus haid atau menstruasi. Kegiatan pengabdian kepada masyarakat ini bertujuan untuk meningkatkan pengetahuan kesehatan reproduksi remaja putri pada siswi di Islam At-Tawwabiin Cilangkap Tapos Depok. Metode yang digunakan dalam program pengabdian ini adaah penyuluhan. Metode survei melalui kuisioner digunakan untuk mengukur sikap dan pemberian soal pre-test dan post-test untuk mengukur pengetahuan. Kegiatan dilaksanakan pada tanggal 27 Juli 2022 dari pukul 08.00-12.00 yang dihadiri oleh 82 siswi SMP Islam At-Tawwabiin. Hasil program kegiatan pengabdian ini menunjukkan sebanyak 95% peserta telah mengalami menstruasi. Usia menstruasi pertama peserta berada pada kisaran 9-14 tahun dengan frekuensi tertinggi pada usia 12 tahun sebanyak 50%. Frekuensi peserta yang mengetahui organ dan system reproduksi sebesar 85,5% dan 54% peserta memahami siklus menstruasi. Berdasarkan hasil evaluasi kegiatan, terjadi peningkatan rataan nilai post-test sebesar 71,83±16,26 dari rataan nilai pre-test sebesar 67,2±16,52 (sig<0,05). Hasil uji korelasi Pearson menunjukkan nilai 0,641 dengan sig 0,003<0,050 yang berarti terjadi hubungan yang kuat dan signifikan antara pengetahuan dengan peningkatan nilai hasil test. Dapat disimpulkan bahwa kegiatan penyuluhan mampu meningkatkan kesadaran dan pengetahuan siswi terhadap kesehatan reproduksi. Kata Kunci: kesehatan reproduksi, menstruasi, siswi SMP, tingkat pengetahuan [English]: Reproductive health in a teenager is greatly influenced by the level of knowledge about reproductive organs and systems. Adolescence is a critical age where young women first experience a phase of puberty which is characterized by the start of the menstrual /menstrual cycle. This community service program aims to increase the awareness and knowledge of young women’s reproductive health in female students at Islam At-Tawwabiin Cilangkap Tapos Depok. The method used in this program is counselling. The survey method with a questionnaire is used to measure the attitude of the participants and the pre-test and post-test questions for knowledge. The program was held on July 27, 2022, from 08.00-12.00 and was attended by 82 At-Tawwabiin Islamic Junior High School students. The results show that 95% of the participants had undergone menstruation. The first menstrual age of participants was in the range of 9-14 years, with the highest frequency at 12 years as much as 50%. The frequency of participants who knew the organs and reproductive system was 85.5%, and 54% understood the menstrual cycle. There was an increase in the average post-test value of 71.83±16.26 from the average pre-test value of 67.2±16.52 (sig<0.05). The results of the Pearson correlation test show a value of 0.641 with a sig of 0.003 < 0.050 , which means a strong and significant relationship exists between knowledge and an increase in test result scores. It can be concluded that the counseling program increased the students’ awareness and knowledge of reproductive health. Keywords: reproductive health, menstruation, junior high school students, knowledge

Effects of Ganoderma lucidum Extract on Diabetic Rats Nuniek Ina Ratnaningtyas; Hernayanti Hernayanti; Suci Andarwanti; Nuraeni Ekowati; Endang Sri Purwanti; Dalia Sukmawati
Biosaintifika: Journal of Biology & Biology Education Vol 10, No 3 (2018): December 2018
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v10i3.15356

Diabetes mellitus (DM) is a metabolic syndrome which occurs when insulin is insufficiently produced or insulin cannot well serve its function. Diabetes is marked with increase in blood glucose level followed by increase in glycosylated hemoglobin level and decrease in insulin level. This research aims to examining the effect of Ganoderma lucidum extract on the blood glucose, insulin and glycosylated hemoglobin (HbA1c) level of diabetic white rat and determining the most effective dose of extract to be a diabetic agent. This research was experimentally conducted by employing Completely Randomized Design (CRD) with 6 treatments and 4 repetitions. The treatment groups consisted of healthy rats group (K1), rats with diabetes as negative control (K2), diabetic rats with the administration of metformin 45 mg/kg BW as the comparison (K3) and diabetic rats with the administration of G. lucidum extract with dose of 250, 500 and 1000 mg /kg BW (K4, K5 and K6 respectively). Blood glucose level examination was conducted after the alloxan induction with single dose of 125 mg/kg BW by intraperitoneal injection. The results show that mushroom G. lucidum extract administration with dose of 1000 mg/kg BW (K6) is the best dose to be an anti-diabetic agent. The benefit of the research is developing anti-diabetic agent from herbal resources.

Isolation and Screening Microorganisms from Black Soldier Fly Larvae (Hermetia illucens) that Producing Amylase, Protease and Cellulase Dalia Sukmawati; Rheva Amadea; Putri Novitasari; Yohana Caroline Sihombing; Axel Mareta; Yohannes Eka Cordias Buulolo; Atin Supiyani; Muktiningsih Nurdjayadi; Diat Nurhidayat; Roshanida A. Rahman; Sulistiyani; Raden Haryo Bimo Setiarto
Jurnal Penelitian Pendidikan IPA Vol 9 No 11 (2023): November
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i11.4516

The Black Soldier Fly (BSF) has gained significant attention for its ability to decompose various types of organic waste and produce valuable enzymes related to its digestive system. These enzymes hold immense potential for applications across diverse industrial sectors, with promise in the livestock industry. This study aimed to extract and assess amylase, protease, and cellulase enzymes from microorganisms present in BSF larvae fermentation. The isolation technique employed was the spread plate method, followed by rigorous screening for the presence of these enzymes. The results showed 46 isolates of 26 bacterial isolates and 20 yeast isolates. In the amylase enzyme screening, a substantial 25 bacterial and 19 yeast isolates exhibited positive amylase activity. For cellulase, 20 bacterial and 14 yeast isolates displayed positive results. In the case of protease, 16 bacterial and 12 yeast isolates demonstrated protease enzyme activity. Notably, nine isolates exhibited the remarkable capability to produce multiple enzymes, including eight bacterial and one yeast isolate. These results showcase the rich enzymatic potential of BSF-associated microorganisms, offering exciting prospects for their application in various industrial sectors, especially in enhancing the efficiency and sustainability of livestock production

Rapid detection of Vibrio alginolyticus in seafood using the flgL gene and real-time polymerase chain reaction Muktiningsih Nurjayadi; Gladys Indira Putri; Jefferson Lynford Declan; Ismaya Krisdawati; Dandy Akbar Juliansyah; Maharanianska Azzahra; Irvan Maulana; Irma Ratna Kartika; Fera Kurniadewi; Tiara Fahriza; Adinda Myra Amalia Putri; Ayu Berkahingrum; Atikah Nur Rahmawati; Rosita Gio Anggraeni; Dalia Sukmawati; Sri Rahayu; Vira Saamia; I Made Wiranatha; Bassam Abomoelak; Hesham Ali El-Enshasy
Acta Biochimica Indonesiana Vol. 7 No. 1 (2024): Acta Biochimica Indonesiana
Publisher : Indonesian Society for Biochemistry and Molecular Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.32889/actabioina.159

Background: Seafood is highly nutritious but poses health risks when contaminated with pathogenic bacteria like Vibrio alginolyticus, which causes food poisoning and can infect marine animals and humans. Objective: This research aimed to determine the sensitivity and specificity of real-time polymerase chain reaction (rt-PCR) using the flgL primer pair to detect V. alginolyticus bacteria in seafood. Methods: The rt-PCR method was used to detect V. alginolyticus quickly, specifically, and sensitively. The flgL primer pair was evaluated for amplicon length, Ct value, Tm value, and its ability to differentiate between target and non-target bacteria. In this research, the samples tested were red snapper and blood clams. Results: The flgL primer produced an amplicon length of 224 bp. At 50 ng concentration, it yielded a Ct value of approximately 11.00 and a Tm of approximately 83°C. The flgL primer successfully differentiated between target and non-target bacteria. In sensitivity tests, it detected V. alginolyticus at concentrations as low as 1.86 x 10-3 ng/µL. Detection in seafood samples was also successful. Conclusion: The rt-PCR assay using the flgL primer pair effectively detects Vibrio alginolyticus in seafood with high specificity, sensitivity, and rapidity. These findings support its use for rapid and accurate detection of pathogenic bacteria in seafood.

Efek Susu Sinbiotik terhadap Struktur Mukosa Duodenum Mencit yang Diinduksi Minyak Trans Dosis Tinggi: Penelitian Hewan Coba Supiyani, Atin; Agussetiandari, Indah; Handayani, Tri; Sukmawati, Dalia
Health Information : Jurnal Penelitian Vol 15 No 1 (2023): Januari-April
Publisher : Poltekkes Kemenkes Kendari

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36990/hijp.v15i1.673

Susu sinbiotik merupakan susu fermentasi probiotik yang dikombinasikan dengan prebiotik. Minyak trans merupakan minyak/lemak dengan struktur trans. Minyak trans yang dikonsumsi secara berlebih dan terus menerus dapat berdampak pada sistem pencernaan. Penelitian ini bertujuan mengetahui pengaruh susu sinbiotik (SS) mengandung tepung pisang tanduk (TP) terhadap struktur mukosa duodenum mencit yang diinduksi minyak trans (MT). Sebanyak 30 ekor mencit jantan strain DDY usia 2 bulan dibagi menjadi 6 kelompok yaitu MT; MT+simvastatin dosis 0,4 mg/20grBB; MT+SS; MT+SS(1%TP); MT+SS(3%TP); dan MT+SS(5%TP). Minyak trans dosis 200mg/20grBB diberikan pada hari 1-14. Susu sinbiotik dosis 200mg/20grBB diberikan pada hari 15-28. Hari ke-29 semua mencit diterminasi secara dekapitasi servikal dan organ duodenum diambil untuk dibuat preparat histologis. Morfologi mukosa duodenum dianalisis secara deskriptif. Secara morfologi duodenum ditemukan erosi vili dan deskuamasi epitel mukosa. Tingkat erosi dan deskuamasi epitel mukosa duodenum dari tinggi-rendah berturut-turut yaitu kelompok MT, MT+Simvastatin dan MT+SS. Semakin tinggi persentase tepung pisang semakin menurunkan tingkat erosi vili dan deskuamasi epitel mukosa duodenum. Pada kelompok susu sinbiotik dengan 5% tepung pisang tidak ditemukan erosi vili dan deskuamasi epitel duodenum. Maka dapat disimpulkan bahwa susu sinbiotik memperbaiki morfologi mukosa duodenum dari erosi vili dan deskuamasi epitel yang diakibatkan oleh minyak trans.

Pemanfaatan Limbah Kulit Biji Kakao (Theobroma Cacao L.) dalam Pembuatan Cookies bagi Masyarakat Pekan Nanas, Johor Bahru, Malaysia Sukmawati, Dalia; Rahayu, Sri; Supiyani, Atin; Supriyatin, Supriyatin; Nabila, Dasilva Azka; Zahra, Lamya Nauratu; Artanti, Guspri Devi; Setiarto, R. Haryo Bimo; Nurhidayat, Diat; El Enshasy, Hesham Ali
Wikrama Parahita : Jurnal Pengabdian Masyarakat Vol. 8 No. 2 (2024): November 2024
Publisher : Universitas Serang Raya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30656/jpmwp.v8i2.7484

Malaysia merupakan peringkat pertama negara penerima ekspor kakao terbanyak dari Indonesia dengan volume mencapai 55,91 ribu ton atau sekitar 14% dari keseluruhan volume ekspor. Pemanfaatan limbah kulit biji kakao saat ini belum maksimal karena dinilai kurang ekonomis. Potensi dari pemanfaatan limbah kulit biji kakao yaitu dapat dijadikan sebagai usaha rumah tangga dengan nilai jual yang cukup tinggi dan keuntungan yang baik. Tujuan pengabdian pada masyarakat (PKM) ini adalah (1) memberikan wawasan kepada Single Mother Community di Pusat Komuniti Desa (PKD) Pekan Nanas, Pontian mengenai pengolahan limbah kulit biji kakao menjadi olahan pangan; (2) memberikan pelatihan kepada Single Mother Community di Pusat Komuniti Desa (PKD) Pekan Nanas, Pontian dalam membuat cookies kulit biji kakao. Metode pelatihan yang digunakan adalah metode pendekatan dan demonstrasi dengan pendampingan praktik. Kegiatan pelatihan diakhiri dengan pengisian kuesioner uji hedonik untuk menilai kualitas produk yang telah dibuat. Hasil PKM yang dicapai adalah peningkatan dalam aspek (1) Wawasan Single Mother Community dalam pengolahan cookies kulit biji kakao dengan teknik pemanggangan meningkat, menunjukkan rata-rata score gain 0,5 dengan peningkatan pengetahuan sebesar 45% dari rata-rata nilai pre-test sebesar 47,1 dan post-test sebesar 70,8 dengan kategori “sedang” (2) Produk cookies kulit biji kakao disukai oleh peserta dengan skor penilaian pada aspek warna 4,7; aroma 4,6; rasa 4,4; dan tekstur 4,5.

Development of Bacillus subtilis Detection Method Targeting Genes codY, narH, and ureC Using Polymerase Chain Reaction Nurjayadi, Muktiningsih; Berkahingrum, Ayu; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Anggraeni, Rosita Gio; Fahriza, Tiara; Declan, Jefferson Lynford; Putri, Gladys Indira; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El Enshasy, Hesham
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 5 No. 1 (2024): JSMARTech Volume 5, No. 1, 2024
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2024.005.01.26

Bacillus subtilis, a causative agent of foodborne illness in bread, cakes, cereals, and cheese leading to symptoms like diarrhea and nausea, was investigated for its role in nosocomial infections, including endocarditis, bacteremia, septicemia, and meningitis. The research focused on three genes: codY, narH, and ureC, which respectively contribute to bacterial survival through biofilm and spore formation, acid resistance, and adaptation to anaerobic conditions. The objective of this study was to evaluate the codY, narH, and ureC genes using the Gradient PCR method for Bacillus subtilis detection in bread and cheese through Real-Time PCR. During the research, bacterial growth was observed with an OD600 of 1.438 in Tryptic Soy Broth (TSB), and colonies of medium size, smooth, cream-coloured, and round shape were successfully isolated on Tryptic Soy Agar (TSA). The DNA template used had a 71.5 ng/µL concentration with an A260/280 ratio of approximately 1.830. The annealing temperature for Gradient PCR used was 53-62°C. The primers successfully amplified codY (175 bp), narH (222 bp), and ureC (153 bp) gene amplicons. The optimal annealing temperature for the primers used was 60°C, as indicated by the presence of a single bright band in electrophoresis. Using these three different genes, testing can also be conducted with the Multiplex PCR method. The next step involves developing a detection kit using optimized primers and annealing temperatures for the identification of Bacillus subtilis in bread and cheese samples using Real-Time PCR.

UJI KEMAMPUAN ANTAGONISME KHAMIR ASAL DAUN JATI (Tectona grandis) TERHADAP KAPANG PENGKONTAMINAN PADA PAKAN TERNAK AYAM: Antagonism Ability Test Yeast Origin Teak Leaves (Tectona grandis) Against Mold Contaminator In Chicken Feed Marham, Hilma Dianti; Rustam, Yoswita; Sukmawati, Dalia
Bioma Vol. 12 No. 2 (2016): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Antagonism is the interaction that occurs when a microorganism interfere with the growth of other microorganisms. One of the microorganisms that have antagonistic ability is yeast. The research aims to determine the ability of antagonism yeast derived from the leaves of teak (Tectona grandis) as an agent for controling mold from poultry feed is Aspergillus sp., Fusarium sp., and Penicillium sp. Tests carried out using the method of antagonism co-culture in medium Potato Dextrose Broth (PDB) during the four days of incubation temperature of 27-28Â°C. The results showed that yeasts have antagonism activity against Aspergillus sp., Fusarium sp., and Penicillium sp. shown by mycelial growth inhibition and sporulation. Co-culture method results obtained 5 potential yeast isolates (T3D2 DU 1.7, T4D2 WU2J2, T4D2 DU 2.1, T4D1 DU 2.1, T5D1 DU 2.2).

ISOLASI DAN SELEKSI KHAMIR AMILOLITIK ASAL BUAH NANGKA (Artocarpus heterophyllus Lam.) Wulandari, Tria Putri; Sukmawati, Dalia; Kurniati, Tri Handayani
Bioma Vol. 13 No. 1 (2017): Bioma
Publisher : LPPM Universitas Negeri Jakarta

The aim of this research is to find the isolates of yeast that have the ability to produce enzyme amylase. The strains that can produce the amylase enzyme are characterized by a clear zone around colonies after addition of iodine solution in medium containing 1% starch soluble. Activity of amylase enzyme can be determined by measuring using spectrophotometer at Î» 540 nm. The isolation result obtained 75 representative yeast isolates with colony color white butyrous 16%, white mucoid 29.3%, yellowish white 18.7%, cream 20%, peach mucoid 9.3%, and orange mucoid 6,7%. Screening results showed that 8 isolates were able to produce an amylase enzyme with code isolates K33, K34, K36, K37, K48, K107, and K128. A total of two potential yeast isolates in yielding amylase with K34 and K39 isolate codes had amylolytic index 2.89 and 2.27. The highest enzyme activity was produced by K48 (0.88 U/mL).

Co-Authors . HERNAYANTI A.S. Satyaningtijas Abomoelak, Bassam Abumoelak, Bassam Adinda Myra Amalia Putri Agung Gumelar Agussetiandari, Indah Akbar, Dandy Andarwanti, Suci Anggraeni, Rosita Gio Atikah Nur Rahmawati Atin Supiyani Atin Supiyani Axel Mareta Ayu Berkahingrum Azzahra, Maharanianska Balqis, Mutia Balqis Bassam Abomoelak Berkahingrum, Ayu Dandy Akbar Juliansyah Daniel Ramadhan Diat Nurhidayat Dwi Ningsih Susilowati El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Endang Sri Purwanti Endang Sri Purwanti Fahriza, Tiara Fera Kurniadewi Fikriyyah, Nabilah Nov Fitriyanti, Anisa Gladys Indira Putri Guspri Devi Artanti Hanifia Rizky HD Marham Hernayanti Hernayanti Hesham Ali El-Enshasy I Made Wiranatha Iman Hidayat Iman Hidayat Irma Ratna Kartika Irvan Maulana Ismaya Krisdawati Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna koekoeh santoso Krisdawati, Ismaya Kurniadewi, Fera Lita Citra Dewi Maharanianska Azzahra Marham, Hilma Dianti Maulana, Irvan Medita, Havana Zahra Moersilah, Moersilah Muktiningsih Nurjayadi Musie, Royna Rahma Nabila, Dasilva Azka NASTITI KUSUMORINI Nuniek Ina Ratnaningtyas Nur Afifah, Zakiah Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Pinkan Amanda Putri Priyo Wahyudi Puspitasari, Sherly Indah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira R Rusdi R. Haryo Bimo Setiarto, R. Haryo Bimo RADEN HARYO BIMO SETIARTO Raden Haryo Bimo Setiarto Rahmawati, Atikah Nur Retno Widowati Rheva Amadea Rini Fahmi, Melta Rista Putri Nur Ifa Roshanida A. Rahman Rosita Gio Anggraeni Rusdi Rusdi Rusilanti Rusilanti Rustam, K. Yoswita RUSTAM, YOSWITA Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Sari, Dessy Putriana Sekar Liyundzira Shabrina, Andisa Shangkara, Muhammad Arkent Sri Rahayu Sri Rahayu SRI RAHAYU Suci Andarwanti Sulistiyani Supiyani, Atin Supiyani, Atin Supiyani Supriyatin Supriyatin Tiara Fahriza Tri Handayani Tri Handayani Kurniati Umiatin Vira Saamia Wiranatha, I Made Wulandari, Tria Putri Yadi Suryadi Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yunita Sari Zahra, Lamya Nauratu

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