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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia VALENSI Biospecies Jurnal MIPA AL KAUNIYAH Buletin Plasma Nutfah Transformasi: Jurnal Pengabdian Masyarakat Unnes Science Education Journal Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Jurnal Mikologi Indonesia Bioma : Jurnal Biologi Indonesia Biosaintifika: Journal of Biology & Biology Education Acta Biochimica Indonesiana Health Information : Jurnal Penelitian Jurnal Riset Sains dan Kimia Terapan Journal of Microbial Systematics and Biotechnology JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Pengabdian kepada Masyarakat Nusantara Aptekmas : Jurnal Pengabdian Kepada Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Buitenzorg: Journal of Tropical Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Dalia Sukmawati
Universitas Negeri Jakarta
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DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira; Nurjayadi, Muktiningsih; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Fahriza, Tiara; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Saamia4, Vira; Saputro, Dwi Anna Oktaviani; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2976

Abstract

Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method. 
Co-Authors . HERNAYANTI A.S. Satyaningtijas Abomoelak, Bassam Abumoelak, Bassam Adinda Myra Amalia Putri Agung Gumelar Agussetiandari, Indah Akbar, Dandy Andarwanti, Suci Anggraeni, Rosita Gio Atikah Nur Rahmawati Atin Supiyani Atin Supiyani Axel Mareta Ayu Berkahingrum Azzahra, Maharanianska Balqis, Mutia Balqis Bassam Abomoelak Berkahingrum, Ayu Dandy Akbar Juliansyah Daniel Ramadhan Diat Nurhidayat Dwi Ningsih Susilowati El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Endang Sri Purwanti Endang Sri Purwanti Fahriza, Tiara Fera Kurniadewi Fikriyyah, Nabilah Nov Fitriyanti, Anisa Gladys Indira Putri Guspri Devi Artanti Hanifia Rizky HD Marham Hernayanti Hernayanti Hesham Ali El-Enshasy I Made Wiranatha Iman Hidayat Iman Hidayat Irma Ratna Kartika Irvan Maulana Ismaya Krisdawati Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna koekoeh santoso Krisdawati, Ismaya Kurniadewi, Fera Lita Citra Dewi Maharanianska Azzahra Marham, Hilma Dianti Maulana, Irvan Medita, Havana Zahra Moersilah, Moersilah Muktiningsih Nurjayadi Musie, Royna Rahma Nabila, Dasilva Azka NASTITI KUSUMORINI Nuniek Ina Ratnaningtyas Nur Afifah, Zakiah Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Pinkan Amanda Putri Priyo Wahyudi Puspitasari, Sherly Indah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira R Rusdi R. Haryo Bimo Setiarto, R. Haryo Bimo RADEN HARYO BIMO SETIARTO Raden Haryo Bimo Setiarto Rahmawati, Atikah Nur Retno Widowati Rheva Amadea Rini Fahmi, Melta Rista Putri Nur Ifa Roshanida A. Rahman Rosita Gio Anggraeni Rusdi Rusdi Rusilanti Rusilanti Rustam, K. Yoswita RUSTAM, YOSWITA Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Sari, Dessy Putriana Sekar Liyundzira Shabrina, Andisa Shangkara, Muhammad Arkent Sri Rahayu Sri Rahayu SRI RAHAYU Suci Andarwanti Sulistiyani Supiyani, Atin Supiyani, Atin Supiyani Supriyatin Supriyatin Tiara Fahriza Tri Handayani Tri Handayani Kurniati Umiatin Vira Saamia Wiranatha, I Made Wulandari, Tria Putri Yadi Suryadi Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yunita Sari Zahra, Lamya Nauratu