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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Pertanian Indonesia VALENSI Biospecies Jurnal MIPA AL KAUNIYAH Buletin Plasma Nutfah Transformasi: Jurnal Pengabdian Masyarakat Unnes Science Education Journal Wikrama Parahita : Jurnal Pengabdian Masyarakat Jurnal Penelitian Pendidikan IPA (JPPIPA) Biosfer: Jurnal Pendidikan Biologi Jurnal Mikologi Indonesia Bioma : Jurnal Biologi Indonesia Biosaintifika: Journal of Biology & Biology Education Acta Biochimica Indonesiana Health Information : Jurnal Penelitian Jurnal Riset Sains dan Kimia Terapan Journal of Microbial Systematics and Biotechnology JSMARTech : Journal of Smart Bioprospecting and Technology Jurnal Pengabdian kepada Masyarakat Nusantara Aptekmas : Jurnal Pengabdian Kepada Masyarakat Prosiding Seminar Nasional Pengabdian Kepada Masyarakat Buitenzorg: Journal of Tropical Science Jurnal Bioteknologi & Biosains Indonesia (JBBI) Jurnal Bioteknologi & Biosains Indonesia
Dalia Sukmawati
Universitas Negeri Jakarta
Religion Aerospace Engineering Agriculture, Biological Sciences & Forestry Arts Humanities Astronomy Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Library & Information Science Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Transportation Veterinary Other

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ISOLASI DAN UJI PATOGENITAS KAPANG PERUSAK PADA APEL MALANG (Malus sylvestris Mill.) PASCA PANEN Shabrina, Andisa; Sukmawati, Dalia; Hidayat, Iman
Bioma Vol. 14 No. 1 (2018): Bioma
Publisher : LPPM Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/Bioma14(1).4

Abstract

ABSTRAK Penelitian ini bertujuan untuk mengisolasi dan menguji patogenitas kapang perusak pada buah apel bergejala busuk. Sampel buah apel busuk berasal dari Pasar Perumnas, Klender, Jakarta Timur. Isolasi dilakukan dengan teknik tanam langsung yang sebelumnya dilakukan proses sterilisasi permukaan kemudian ditanam di media PDA. Uji patogenitas berdasarkan Postulat Koch dengan cara kapang-kapang hasil isolasi diinfeksikan ke buah apel segar. Hasil isolasi sebanyak 18 isolat kapang berhasil diisolasi dari buah apel bergejala busuk. Isolat kapang yang diperoleh memiliki koloni yang berbeda yaitu, koloni bersporulasi hijau granul, koloni bersporulasi koloni hitam granul, koloni bersporulasi abu-abu, dan koloni bermiselium abu-abu. Isolat dengan kode A1 dengan ciri memiliki sporulasi hitam merupakan isolat kapang paling perusak buah apel. Hal ini diketahui berdasarkan uji patogenitas yaitu nilai Keterjadian Penyakit (KP) sebesar 100% dan Keparahan Penyakit (KeP) sebesar 50%. Kata kunci: buah busuk, kapang pembusuk, Postulat koch
Detection of the Yersinia enterocolitica Bacteria Targeting the myfA and ystA Genes in Contaminated Vegetable Samples using Real-Time PCR to Develop Rapid Detection of Food Poisoning Bacteria Nurjayadi, Muktiningsih; Anggraeni, Rosita GIo; Putri, Gladys Indira; Declan, Jefferson Lynford; Juliansyah, Dandy Akbar; Fahriza, Tiara; Putri, Adinda Myra Amalia; Berkahingrum, Ayu; Rahmawati, Atikah Nur; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
HAYATI Journal of Biosciences Vol. 32 No. 4 (2025): July 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.4.989-1002

Abstract

Yersinia enterocolitica is a pathogenic bacterium with the ability to survive and multiply in food in a low-temperature environment that can cause death in humans. In previous studies, the optimum annealing temperature of ymoA, ystA, and ail gene primers with amplicons of 185 bp, 123 bp, and 192 bp, respectively, was successfully found. This study aims to develop a pathogenic bacteria detection kit with confirmation, sensitivity, and specificity of myfA and ystA primers in detecting Yersinia enterocolitica bacteria quickly and accurately using the real-time Polymerase Chain Reaction method. The results showed that myfA and ystA primers have optimum annealing temperatures at 60°C with amplicon lengths of 181 bp and 123 bp, respectively. Primer myfA was able to amplify the target with real-time PCR at Ct 12.07±1 and Tm 81±1°C, while the ystA primer at Ct 12.38±1 and Tm 83±1°C. myfA and ystA primers were also able to distinguish target and non-target bacteria based on Ct or Tm. The designed primers successfully detected Yersinia enterocolitica bacteria with the smallest concentration of 0.000439 ng/µL equivalent to 7.024 × 102 CFU. The detection limit obtained is smaller than the contamination threshold set by the Food and Drug Administration (BPOM). Primer myfA and ystA Yersinia enterocolitica also successfully detected the target bacteria in cabbage and lettuce samples artificially. Based on these results, myfA and ystA primers successfully detected Yersinia enterocolitica in vegetable samples using real-time PCR quickly, sensitively, specifically, and accurately.
PENGARUH SUBSTITUSI TEPUNG KULIT BIJI KAKAO PADA SOFT COOKIES TERHADAP KANDUNGAN GIZI DAN DAYA TERIMA KONSUMEN ANAK USIA SEKOLAH DASAR Medita, Havana Zahra; Rusilanti, Rusilanti; Sukmawati, Dalia
Biospecies Vol. 16 No. 2 (2023): Juli 2023
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v16i2.29934

Abstract

This research aims to analyze the effect of substitution of cocoa bean shell flour in soft cookies on the nutritional content and consumer acceptability of elementary school age children. The samples in this research were soft cookies substituted for cocoa bean shell flour with three treatments, namely percentages of 10%, 20% and 30%. This research was carried out in the initial stages, namely proximate testing of the nutritional content of soft cookies and organoleptic testing carried out by 20 untrained panelists from Bani Saleh 1 Elementary School students and 20 untrained panelists from Margahayu VIII Elementary School students with assessment aspects including taste, color, aroma, and texture. The results of the proximate test data show that soft cookies meet the national standard quality requirements for nutritional content, namely protein content and fat content. The results of Friedman's test data show that the soft cookies that many students at Bani Saleh 1 Elementary School like are soft cookies with a substitution percentage of 10% with an average value for the taste aspect of 3.80, the aroma aspect of 3.50, and the texture aspect of 3.60. Meanwhile, Friedman's test data shows that the soft cookies that many students at SDN Margahayu VIII like are soft cookies with a substitution percentage of 30% with an average value for the taste aspect of 3.60 and the texture aspect of 3.65. The conclusion of this research is that the best formula for soft cookies substituted for cocoa bean shell flour based on organoleptic tests in two elementary schools and proximate tests that have been carried out is soft cookies with 10% substitution. Keywords: Cocoa Bean Shell Flour, Consumer Acceptability, Nutritional Content, and Soft Cookies   Abstrak Penelitian ini bertujuan untuk menganalisis pengaruh substitusi tepung kulit biji kakao pada soft cookies terhadap kandungan gizi dan daya terima konsumen anak usia sekolah dasar. Sampel dalam penelitian ini adalah soft cookies subtitusi tepung kulit biji kakao dengan tiga perlakuan, yaitu persentase 10%, 20%, dan 30%. Penelitian ini dilakukan dengan tahapan awal adalah pengujian proksimat dari kandungan gizi soft cookies  dan pengujian organoleptik yang dilakukan oleh 20 orang panelis tidak terlatih siswa SD Bani Saleh 1 dan 20 orang panelis tidak terlatih siswa SDN Margahayu VIII dengan aspek penilaian meliputi aspek rasa, warna, aroma, dan tekstur. Hasil dari data uji proksimat menunjukkan bahwa soft cookies yang sudah memenuhi persyaratan mutu standar nasional kandungan gizi adalah kadar protein dan kadar lemak. Hasil dari data uji Friedman menunjukkan bahwa soft cookies  yang banyak disukai siswa dan siswi SD Bani Saleh 1 adalah soft cookies dengan persentase substistusi 10% dengan nilai rata-rata aspek rasa 3,80, aspek aroma 3,50, dan aspek tekstur 3,60. Sedangkan dari data uji Friedman menunjukkan bahwa soft cookies yang banyak disukai siswa dan siswi SDN Margahayu VIII adalah soft cookies dengan persentase substistusi 30% dengan nilai rata-rata aspek rasa 3,60 dan aspek tekstur 3,65. Kesimpulan pada penelitian ini bahwa formula terbaik dari soft cookies substitusi tepung kulit biji kakao berdasarkan uji organoleptik di dua sekolah dasar dan uji proksimat yang telah dilakukan adalah soft cookies dengan substitusi 10%. Kata Kunci: Daya Terima Konsumen, Kandungan Gizi, Soft Cookies, dan Tepung Kulit Biji Kakao.
Optimization of Information and Communication Technology: Digital Innovation for Sustainable Agriculture Nurhidayat, Diat; Sukmawati, Dalia
Biospecies Vol. 17 No. 2 (2024): Juli 2024
Publisher : Universitas Jambi

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22437/biospecies.v17i2.36710

Abstract

The development of the times in the generation Z era, uses information technology so much that it cannot be separated from today’s daily life. This requires us to increasingly utilize this technology to provide positive impacts rather than negative effects. One use that can be used in the field of biology is to provide digital innovation to help people understand how to learn about oyster mushroom cultivation by the community. Fungi are a group of multicellular eukaryotic fungi with body parts consisting of a base. The group of fungi that have a basidium or are known as fruiting bodies are a group of macroscopic fungi. This group of macroscopic fungi has very high nutritional value. High protein and cellulase are one of the nutritional values contained in these fungi. Macroscopic fungi are often found in forest areas or plantations in rural areas. Edible mushrooms are a food ingredient that contains complete nutrition. Fungi can also be used as a medicinal ingredient. Ling-zhi fungus (Ganoderma sp.) is an ingredient in anticancer drugs, increasing fitness, lowering blood sugar, lowering cholesterol, destroying carcinogens, and antibacterial. Shiitake (Lentinus edodes), maitake (Grifola frondosa) and oyster (Pleurotus sp.) mushrooms have been used as antimicrobial, anti-inflammatory, antioxidant, anti-cholesterol, anti-cancer, antiviral and immunomodulator (Wasser, 2002). The benefits and knowledge regarding the potential of large mushrooms have not been well explored. One of the efforts that will be made in community service in Rajadesa village is to provide knowledge to the surrounding community to utilize the potential of natural resources around the village in order to cultivate large mushrooms, namely Pleurotus sp. The potential for villages with cold temperatures can be used as a place for mushroom cultivation coupled with the optimization of simple information technology to assist in the extension process. The results of the technological innovation used in the service will be carried out for two days with the education stage on how important it is to have the ability to cultivate mushrooms. The next stage is counseling regarding techniques for cultivating large mushrooms by utilizing the natural potential that exists in the countryside. As a result of the service, it is hoped that the community will have knowledge about the health benefits of mushrooms, the types of mushrooms that can be cultivated and education regarding mushroom cultivation techniques.
Pemberdayaan Kader Peduli Stunting melalui Penerapan Smart Antropometri “Nimbang Balita” di Posyandu Majalaya, Cianjur. Umiatin; Irma Ratna Kartika; Dalia Sukmawati; Rista Putri Nur Ifa; Pinkan Amanda Putri
Jurnal Pengabdian kepada Masyarakat Nusantara Vol. 6 No. 2 (2025): Jurnal Pengabdian kepada Masyarakat Nusantara Edisi April - Juni
Publisher : Lembaga Dongan Dosen

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55338/jpkmn.v6i2.5331

Abstract

Stunting masih menjadi permasalahan dan tantangan utama di Posyandu Nusa Indah, Desa Majalaya, Kecamatan Cikalong Kulon, Cianjur. Pada tahun 2021 desa ini merupakan salah satu lokus stunting di Cianjur, Jawa Barat. Berbagai upaya dilakukan untuk penurunan dan pencegahan stunting di wilayahnya, antara lain melalui kolaborasi dengan berbagai pemangku kepentingan. Saat ini salah satu kendala yang dihadapi kader posyandu berkaitan dengan sarana dan prasarana adalah keterbatasan alat antropometri yang akurat dan belum optimalnya digitalisasi dalam pengelolaan data. Oleh karena itu, melalui kegiatan pengabdian masyarakat ini dilakukan penerapan smart antropometric “Nimbang Balita” yang merupakan hasil penelitian tim peneliti. Kegiatan pengabdian masyarakat ini bertujuan untuk mewujudkan digitalisasi posyandu serta meningkatkan pengetahuan dan ketrampilan kader. Kegiatan ini dilakukan pada bulan Oktober sampai dengan November 2024 di Posyandu Nusa Indah 3, Desa Majalaya, Kecamatan Cikalong Kulon, Cianjur. Metode pelaksanaan pengabdian masyarakat ini terdiri empat tahap yaitu : survei, persiapan, pelaksanaan dan evaluasi. Kegiatan ini diikuti oleh 5 orang kader Posyandu Nusa Indah 3, satu orang bidan, serta 30 balita. Kegiatan penimbangan menggunakan smart anthropometric “Nimbang Balita” berjalan dengan lancar, namun terdapat beberapa hambatan teknis antara lain jaringan internet yang tidak stabil sehingga mengganggu penggunnaan alat. Berdasarkan hasil evaluasi kegiatan dapat disimpulkan bahwa melalui pengabdian masyarakat ini pengetahuan dan ketrampilan kader posyandu Nusa Indah 3 dalam melakukan pengukuran antropometri meningkat. 
Deteksi Vibrio parahaemolyticus Menggunakan Primer Gen toxR2 dengan Gradient Polymerase Chain Reaction Nurjayadi, Muktiningsih; Krisdawati, Ismaya; Declan, Jefferson Lynford; Putri, Gladys Indira; Juliansyah, Dandy Akbar; Rahmawati, Atikah Nur; Fitriyanti, Anisa; Musie, Royna Rahma; Kurniadewi, Fera; Sukmawati, Dalia; Saamia, Vira; Wiranatha, I Made; Abomoelak, Bassam; Elenshasy, Hesham Ali
Jurnal Riset Sains dan Kimia Terapan Vol. 11 No. 1 (2025): Jurnal Riset Sains dan Kimia Terapan, Volume 11 Nomor 1, Juni 2025
Publisher : Program Studi Kimia Universitas Negeri Jakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21009/JRSKT.111.03

Abstract

Makanan adalah kebutuhan vital, dengan kriteria utama adalah keamanan, kualitas, dan nilai gizi. Untuk memastikan keamanan makanan, diperlukan metode deteksi yang cepat dan akurat, terutama untuk mendeteksi bakteri patogen penyebab keracunan makanan. Vibrio parahaemolyticus merupakan bakteri patogen yang banyak ditemukan pada makanan laut. Penelitian ini bertujuan untuk mengembangkan metode deteksi cepat V. parahaemolyticus dengan menargetkan gen toxR2 menggunakan Gradient Polymerase Chain Reaction. Gen toxR dipilih karena fungsinya sebagai pengatur penting gen virulensi. Tahapan yang dilakukan meliputi desain primer, penyiapan sampel bakteri dari biakan murni, dan uji amplifikasi menggunakan PCR Gradien. Hasil uji amplifikasi menunjukan bahwa pasangan primer toxR2 berhasil mengamplifikasi pada suhu 58-62°C dengan dihasilkan pita berukuran 137 bp. Berdasarkan hasil tersebut, dapat disimpulkan bahwa PCR Gradien dengan primer toxR2 dapat diaplikasikan untuk mengembangkan alat deteksi V. parahaemolyticus dengan dilakukan uji lanjutan seperti uji konfirmasi, uji spesifisitas, uji sensitivitas, dan uji pada pangan menggunakan Real-Time Polymerase Chain Reaction.
Efficacy Test of Prototype Kit for Detection Bacillus cereus and Listeria monocytogenes in Processed Meat using Real-time PCR Method Nurjayadi, Muktiningsih; Fahriza, Tiara; Putri, Adinda Myra Amalia; Rahmawati, Atikah Nur; Berkahingrum, Ayu; Anggraeni, Rosita Gio; Putri, Gladys Indira; Declan, Jefferson Lynford; Akbar, Dandy; Maulana, Irvan; Azzahra, Maharanianska; Shangkara, Muhammad Arkent; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Rahayu, Sri; Saamia, Vira; Wiranatha, I Made; Abumoelak, Bassam; El Enshasy, Hesham
Jurnal Kimia Valensi Jurnal Kimia VALENSI, Volume 11, No. 1, May 2025
Publisher : Department of Chemistry, Faculty of Science and Technology Syarif Hidayatullah Jakarta State Islamic University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15408/jkv.v11i1.44280

Abstract

According to the World Health Organization (WHO), harmful agents such as Bacillus cereus and Listeria monocytogenes are responsible for 600 million cases of disease and 420,000 deaths annually. This research aims to test the effectiveness of the real-time PCR method for developing a prototype kit to detect pathogenic bacteria in processed meat. As a comparison, the and conventional PCR methods were used to obtain the accuracy, specificity, sensitivity, and effectiveness of the real-time PCR method. All the samples were cultured in solid media agar, performed amplification using specific primers cyt-K 2 and hly using PCR and real-time PCR. Meatballs, nuggets, and sausages, five samples each, were found to be positive positively contaminated with all targeted bacteria. However, they did not provide specific results using solid media culture and the PCR method. In addition, the real-time PCR method using prototype kit formulas accomplished that all contaminated samples had a Ct value smaller than the negative control, NTC (No Template Control), and had a similar melting curve to the positive control. This establishes that the real-time PCR method clarifies that all samples were contaminated with target bacteria. A formula was developed in the prototype kit with real-time PCR methods that can be used and applied on a commercial scale efficiently and precisely.
DETERMINATION OF OPTIMAL ANNEALING TEMPERATURE Vibrio alginolyticus PRIMERS USING POLYMERASE CHAIN REACTION METHOD Putri, Gladys Indira; Nurjayadi, Muktiningsih; Declan, Jefferson Lynford; Krisdawati, Ismaya; Juliansyah, Dandy Akbar; Fahriza, Tiara; Azzahra, Maharanianska; Maulana, Irvan; Kartika, Irma Ratna; Kurniadewi, Fera; Sukmawati, Dalia; Saamia4, Vira; Saputro, Dwi Anna Oktaviani; Wiranatha, I Made; Abomoelak, Bassam; El-Enshasy, Hesham Ali
Jurnal Bioteknologi & Biosains Indonesia (JBBI) Vol. 10 No. 2 (2023)
Publisher : BRIN - Badan Riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/jbbi.2023.2976

Abstract

Food poisoning is a global issue of grave concern. If food is not properly cooked, it can be a medium for the spread of pathogenic bacteria. Vibrio alginolyticus is one of the pathogenic bacteria that can cause food poisoning. real-time Polymerase Chain Reaction (rt-PCR) can detect pathogenic bacteria in food, so it is necessary to determine the optimal annealing temperature. This research aims to obtain the optimal annealing temperature of the Va_Chr1_FR primer using Gradient PCR. The DNA concentration used was 174.5 with an A260/A280 purity of 1.94. The temperature range tested, 53°C-62°C, corresponds to the melting temperature of the Va_Chr1_FR primers. The primers designed were F5'-TTCTTCTGTTGTAGGTTCCG-F3' and R5'-CCAGCCCTCACATCTAATAC-R3'. Based on these results, a temperature of 60°C is deemed as the most optimal annealing temperature because it produces one of the brightest bands on electrophoresis with an amplicon length of 146 bp. The findings of this study will be beneficial to the development of Va_Chr1_FR Vibrio alginolyticus primers testing on food samples using the real-time PCR method. 
Pengaruh pemberian pakan fermentasi ampas kelapa dan bekatul padi menggunakan Rhiodiotorula taiwanensis UNJCC Y-171 terhadap pertumbuhan larva Black Soldier Fly (BSF) dan komposisi proksimatnya. Sari, Yunita; Rini Fahmi, Melta; Sukmawati, Dalia
Buitenzorg: Journal of Tropical Science Vol 2 No 1 (2025): Vol. 2 No. 1 (2025): Buitenzorg: Journal of Tropical Science
Publisher : Innovation Centre for Tropical Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.70158/buitenzorg.v2i1.14

Abstract

Black soldier fly (BSF) larvae, or maggots, are a promising alternative protein-rich feed for aquaculture. This study aims to evaluate the effect of fermented feed made from coconut pulp and rice bran using the yeast Rhodotorula taiwanensis UNJCC Y-171 on maggot growth and proximate composition. The study was conducted from June to August 2024 at the Universitas Negeri Jakarta and the Ornamental Fish Aquaculture Research Center. The treatments included a control group (K) and fermentation using yeast at 30% (A) and 40% (B) concentrations. Measurements were taken on days 10, 13, 16, and 19. The results showed that fermented feed significantly increased maggot wet weight and body length, particularly in treatment B. On day 16, the wet weight of maggots in treatment B reached 182.00±45.14 g, higher than the control (77,00±52.03 g). The body length of maggots in treatment B was also greater (1.59±0.12 cm) than in the control (1.19±0.09 cm). Proximate analysis indicated that maggots in treatment B had a higher fat content (15.57%) compared to the control (12.11%), while crude fiber content was lower (1.27% compared to 1.63%), indicating improved feed efficiency. Although the protein content in treatment B (12.29%) was slightly lower than in the control (13.27%), fermentation with R. taiwanensis UNJCC Y-171 enhanced nutrient bioavailability. This study confirms that feed fermentation with R. taiwanensis UNJCC Y-171 at a 40% concentration optimally improves both maggot growth and nutritional quality. Therefore, this method has the potential to serve as a more cost-effective alternative feed for aquaculture. Keywords: Rhodotorula taiwanensis, feed fermentation, maggot, proximate analysis, feed efficiency
Kemampuan Kapang Dark Septate Endophyte dari Akar Tanaman Aren dalam Menghambat Ganoderma sp. Sukmawati, Dalia; Supiyani, Atin Supiyani; Nur Afifah, Zakiah; Balqis, Mutia Balqis; Fikriyyah, Nabilah Nov; Bimo Setiarto, Raden Haryo; Sari, Dessy Putriana
Jurnal Ilmu Pertanian Indonesia Vol. 29 No. 2 (2024): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18343/jipi.29.2.278

Abstract

Ganoderma is a fungal pathogen that can cause rot disease at the base of plant stems. This disease threatens oil palm production, especially in Southeast Asia. Trichoderma sp. fungi can control Basal Stem Rot disease in Ganoderma boninense but are ineffective. One solution that can be done to inhibit the growth of Ganoderma sp. is by using Dark Septate Endophyte (DSE) fungi. Research on DSE isolated from sugar palm (Arenga pinnata Merr.) has not been widely studied. The sugar palm plant is related to the oil palm plant in the Arecaceae family. This study aims to analyze the potential of DSE fungi from the roots of the sugar palm plant in inhibiting the pathogenic fungi Ganoderma sp. The stages of this research were isolation and purification of DSE fungi from sugar palm roots, DSE antagonists against Ganoderma sp., and DSE volatile compounds against Ganoderma sp. The results of isolation and purification showed that there were 18 DSE isolates grouped based on morphological similarities in the form of DSE fungi colony colors, where there were ten fungi isolates representing ten different colony colors, namely, isolate A.3.1 (1); A.3.2 (2); A.4.1 (2); A.4.1 (3); A.4.2; A.5.2; A.5.3; A.6.1(a); A.6.2(a); A.6.4(a). A total of 8 fungi isolates were tested for antagonists and volatile compound testing on Ganoderma sp. The results of the antagonist test showed that DSE A4.1(2) had the highest percentage of inhibition with a value of 36.12%, and isolated A6.2 had the highest percentage of inhibition and the lowest with a value of 15.65%. The testing results for volatile compounds showed that the isolate of DSE A4.2 had the highest percentage of inhibition against Ganoderma sp. at 18.25%. In comparison, the lowest inhibition percentage was obtained from DSE A5.2 fungi isolate with a value of 0.43%. Keywords: dark septate endophyte, ganoderma, sugar palm (Arenga pinnata Merr.)
Co-Authors . HERNAYANTI A.S. Satyaningtijas Abomoelak, Bassam Abumoelak, Bassam Adinda Myra Amalia Putri Agung Gumelar Agussetiandari, Indah Akbar, Dandy Andarwanti, Suci Anggraeni, Rosita Gio Atikah Nur Rahmawati Atin Supiyani Atin Supiyani Axel Mareta Ayu Berkahingrum Azzahra, Maharanianska Balqis, Mutia Balqis Bassam Abomoelak Berkahingrum, Ayu Dandy Akbar Juliansyah Daniel Ramadhan Diat Nurhidayat Dwi Ningsih Susilowati El Enshasy, Hesham El Enshasy, Hesham Ali El-Enshasy, Hesham Ali Elenshasy, Hesham Ali Endang Sri Purwanti Endang Sri Purwanti Fahriza, Tiara Fera Kurniadewi Fikriyyah, Nabilah Nov Fitriyanti, Anisa Gladys Indira Putri Guspri Devi Artanti Hanifia Rizky HD Marham Hernayanti Hernayanti Hesham Ali El-Enshasy I Made Wiranatha Iman Hidayat Iman Hidayat Irma Ratna Kartika Irvan Maulana Ismaya Krisdawati Jefferson Lynford Declan Juliansyah, Dandy Akbar Kartika, Irma Ratna koekoeh santoso Krisdawati, Ismaya Kurniadewi, Fera Lita Citra Dewi Maharanianska Azzahra Marham, Hilma Dianti Maulana, Irvan Medita, Havana Zahra Moersilah, Moersilah Muktiningsih Nurjayadi Musie, Royna Rahma Nabila, Dasilva Azka NASTITI KUSUMORINI Nuniek Ina Ratnaningtyas Nur Afifah, Zakiah Nuraeni Ekowati Nuraeni Ekowati, Nuraeni Pinkan Amanda Putri Priyo Wahyudi Puspitasari, Sherly Indah Putri Novitasari Putri, Adinda Myra Amalia Putri, Gladys Indira R Rusdi R. Haryo Bimo Setiarto, R. Haryo Bimo RADEN HARYO BIMO SETIARTO Raden Haryo Bimo Setiarto Rahmawati, Atikah Nur Retno Widowati Rheva Amadea Rini Fahmi, Melta Rista Putri Nur Ifa Roshanida A. Rahman Rosita Gio Anggraeni Rusdi Rusdi Rusilanti Rusilanti Rustam, K. Yoswita RUSTAM, YOSWITA Saamia, Vira Saamia4, Vira Saputro, Dwi Anna Oktaviani Sari, Dessy Putriana Sekar Liyundzira Shabrina, Andisa Shangkara, Muhammad Arkent SRI RAHAYU Sri Rahayu Sri Rahayu Suci Andarwanti Sulistiyani Supiyani, Atin Supiyani, Atin Supiyani Supriyatin Supriyatin Tiara Fahriza Tri Handayani Tri Handayani Kurniati Umiatin Vira Saamia Wiranatha, I Made Wulandari, Tria Putri Yadi Suryadi Yohana Caroline Sihombing Yohannes Eka Cordias Buulolo Yunita Sari Zahra, Lamya Nauratu