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All Journal HAYATI Journal of Biosciences Jurnal Pengolahan Hasil Perikanan Indonesia Jurnal Ilmu dan Teknologi Kelautan Tropis ILMU KELAUTAN: Indonesian Journal of Marine Sciences BULETIN OSEANOGRAFI MARINA Jurnal Natur Indonesia Jurnal Pengolahan dan Bioteknologi Hasil Perikanan Journal of Marine Research Jurnal Harpodon Borneo Jurnal Kelautan : Indonesian Journal of Marine Science and Technology Journal of Aquatropica Asia Jurnal Kelautan Tropis Panrita Abdi - Jurnal Pengabdian pada Masyarakat JFMR (Journal of Fisheries and Marine Research) JRST (Jurnal Riset Sains dan Teknologi) Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Jurnal Pengelolaan Perairan Biosaintifika: Journal of Biology & Biology Education Indonesian Journal of Natural Pigments Aquacultura Indonesiana Journal of Tropical Marine Science Indonesian Journal of Mollusk JURNAL BIOLOGI PAPUA Journal of Fisheries & Marine Jurnal Ilmu Kelautan Lesser Sunda
Delianis Pringgenies
Jurusan Ilmu Kelautan, Fakultas Perikanan Dan Ilmu Kelautan, Universitas Diponegoro Jl. H. Prof. Sudarto, SH, Tembalang, Semarang, Indonesia 50275
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Decision Sciences, Operations Research & Management Earth & Planetary Sciences Engineering Environmental Science Immunology & microbiology Library & Information Science Materials Science & Nanotechnology Social Sciences Other

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Pewarna Alami Limbah Mangrove Dengan Fiksasi Air Kelapa, Asam Jawa Dan Tawas Alfin Anggraeni; Delianis Pringgenies; Ali Ridlo
Journal of Marine Research Vol 12, No 3 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i3.36450

Abstract

Mangrove termasuk tumbuhan yang dipengaruhi oleh pasang surut air laut, memiliki adaptasi terhadap kadar garam yang cukup tinggi, dan selalu tergenang air. Manfaat mangrove salah satunya digunakan sebagai pewarna alami pada kain. Penerapan pewarna alami dapat mengurangi adanya dampak pencemaran yang disebabkan oleh pewarna sintesis. Penelitian ini bertujuan untuk mengetahui daya tahan luntur kain terhadap pencucian sabun, kelunturan, perbedaan warna, dan warna yang dihasilkan pada kain dari hasil pengujian beda warna kain sebelum dan sesudah pencucian sabun, pada pewarna limbah propagul dan akar mangrove dengan fiksasi air kelapa, asam jawa dan tawas. Penelitian dilakukan pada bulan Juni – Juli 2022. Metode yang digunakan yaitu metode eksperimen, yang terdiri dari ekstraksi zat warna alam mangrove, pencelupan dan fiksasi, serta pengujian kualitas kain yang  terdiri dari uji tahan luntur warna kain terhadap pencucian sabun, pengujian beda warna kain sebelum dan sesudah dilakukannya pencucian sabun, perhitungan perbedaan warna (ΔE) dan analisis warna dengan color analysis, ibisPaint X dan colorimeter. Hasil penelitian menunjukkan bahwa daya tahan luntur pada propagul tergolong baik (4) dan akar tergolong cukup baik (3,9), nilai beda warna kain sebelum dan sesudah pencucian sabun pada akar lebih tinggi daripada propagul, perbedaan warna paling rendah, yaitu air kelapa, dan rata-rata warna propagul lebih gelap dibandingkan akar. Berdasarkan penelitian yang dilakukan, fiksasi yang baik yaitu air kelapa, karena memiliki tingkat kelunturan paling rendah. Mangroves are plants that are affected by tides, have adaptations to high levels of salt, and are always inundated with water. The benefit of one of the mangroves is used as a natural dye on cloth. The application of natural dyes can reduce the impact of pollution caused by the synthesis of dyes. This study aims to determine the resistance of the fabric to washing, fading, color difference, and the color produced on the fabric from the color difference testing before and washing soap, on dyes of propagule waste and mangrove roots with fixation of coconut water, tamarind and alum. The study was conducted in June – July 2022. The method used is the experimental method, which consists of extracting natural mangrove dyes, dyeing and fixation, as well as testing the quality of the fabric which consists of testing the fastness of the fabric to washing soap, testing the color difference before and before execution of washing, calculation of differences (ΔE) and color analysis with color analysis, ibisPaint X and colorimeter. The results showed that the fastness of propagule was good (4) and the roots were quite good (3.9), the difference in the value of the fabric before and washing soap on the roots was higher than that of propagules, the lowest color difference was coconut water, and the average color of the propagules is darker than the roots. Based on the research conducted, a good fixation is coconut water, because it has the lowest level of fastness.
Exploration of Antimicrobial Potency of Mangrove Symbiont Against Multi-Drug Resistant Bacteria Delianis Pringgenies; Wilis Ari Setyati; Ali Djunaedi; Rini Pramesti; Siti Rudiyanti; Dafit Ariyanto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 13 No. 2 (2021): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v13i2.26199

Abstract

Highlight ResearchAntimicrobial potential against the test microbesRhizhopora mucronata isolate showed 95% homology with Bacillus subtilis, and 97% homology with Bacillus oceanisediminis,Acanthus ilicifolius isolate showed 96% homology with Paracoccus caeni, and 89% homology with Bacillus circulans. The study found 4 isolates with antimicrobial potency against MDR pathogenic microbes.The symbiont microbes taken from Rhizophora mucronata and Acanthus ilicifolius were determined to be of the genus Bacillus and Paracoccus AbstractAntimicrobial property of mangrove symbiont have the ability to fight Multi Drug Resistant bacteria which were Staphylococcus aureus, Escherichia coli, and Vibrio haryeyi. This study aimed to determine the potential of symbiont microbes from the root of Rhizopora mucronata and Acanthus iilicifolius as antimicrobial agents against multi-drug resistant (MDR) pathogenic microbes. This research was conducted during July to November 2020. The MDR bacteria were S. aureus, E. coli, and V. harveyi MDR test microbes. The symbiont microbes were identified through molecular analyses (PCR 16S rDNA). Isolation of symbiont microbes from R. mucronata resulted in 16 isolates, while isolation from A. iilicifolius resulted in 14 isolates. Based on the antimicrobial qualitative test against S. aureus, 8 out of 16 microbial isolates from R. mucronata were found to show antimicrobial properties. The testing of A. ilicifolius symbiont microbes against S. aureus showed 8 out of 14 isolates with antimicrobial properties. The test against E. coli resulted in 2 out of 16 microbial isolates from R. mucronata and 5 out of 14 isolates from A. ilicifolius with antimicrobial properties. The test against V. harveyi resulted in two out of 16 microbial isolates from R.mucronata and 4 out of 14 isolates from A. ilicifolius with antimicrobial properties. The quantitative test found 2 isolates from R. mucronta, namely isolates RM10 and RM12, with antimicrobial properties against MDR strain E. coli, with the best isolate being RM10, which produced 11.22 mm of inhibition zone diameter. Furthermore, the selection of isolates was based on the size of the inhibition zone, the clearness of the inhibition zone and the potential for antibacterial activity. Based on their overall antimicrobial potential against the test microbes, four isolates were selected.  Molecular analyses of RM12 isolate showed 95% homology with Bacillus subtilis, of RM 10 isolate showed 97% homology with Bacillus oceanisediminis, of AC isolate showed 96% homology with Paracoccus caeni, and of AC 5 isolate showed 89% homology with Bacillus circulans. The study found four isolates with antimicrobial potency against MDR pathogenic microbes. The symbiont microbes taken from R. mucronata and A. ilicifolius were determined to be of the genus Bacillus and Paracoccus. 
Kandungan Logam Berat Pb dan Cd dalam Sedimen di Pantai Trimulyo dan Pantai Tirang, Semarang Maulina, Dinda Rizky Ayu; Pringgenies, Delianis; Haryanti, Dwi
Journal of Marine Research Vol 13, No 1 (2024): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v13i1.35038

Abstract

Kandungan timbal (Pb) dan kadmium (Cd) di sedimen sebagian berasal dari limbah industri yang masuk ke perairan dan terakumulasi di muara sungai. Pantai Trimulyo berada di pesisir Semarang, dekat dengan Kawasan Industri Terboyo yang berpotensi membuang limbah logam berat ke peraian. Pantai Tirang juga berpotensi tercemar limbah logam berat dari industri di Kecamatan Tugu yang masuk ke aliran Sungai Tapak. Penelitian ini bertujuan untuk mengetahui kandungan logam Pb dan Cd pada sedimen di Pantai Trimulyo dan Pantai Tirang. Penelitian dilakukan dengan metode survei eksploratif dan penentuan lokasi pengamatan dengan metode purposive sampling. Sampel sedimen diambil sebanyak 3 kali pengulangan disetiap lokasi dengan sediment core. Sampel sedimen dianalisis di Laboratorium BBTPPI Semarang menggunakan alat Atomic Absorption Spectrophotometer (AAS) untuk menentukan kadar logam Pb dan Cd dalam sedimen. Hasil analisis kandungan logam Pb dalam sedimen menunjukkan bahwa konsentrasi logam berat tertinggi ditemukan di Pantai Tirang sebesar 49,16 mg/kg dan Pantai Trimulyo memiliki kandungan Pb tertinggi 23,78 mg/kg. Hasil analisis kandungan logam Cd dalam sedimen di Pantai Trimulyo dan Pantai Tirang sebesar >0,050 mg/kg. Pengukuran parameter lingkungan (suhu, salinitas, pH, DO, kecepatan arus dan kecerahan perairan) secara in situ menunjukkan konstribusi terhadap kandungan logam berat pada sedimen. Secara keseluruhan, kandungan logam berat Cd pada sedimen di setiap lokasi penelitian tidak melebihi baku mutu, sedangkan kandungan logam berat pada sedimen yang melebihi atau mendekati baku mutu yaitu logam berat timbal.  The sediments contain lead (Pb) and cadmium (Cd) from industrial waste were often found to enter nearby waters and accumulates at river mouths. Trimulyo Beach is adjacent to the Terboyo Industrial Estate on Semarang's coast, which has the potential to dump heavy metal waste into the waters. Heavy metal waste from industry in Tugu District that enters the Tapak River might also pollute Tirang Beach. The aim of the study is to determine the Pb and Cd content of sediments at Trimulyo and Tirang beaches. The research was conducted by using an exploratory survey method, and the research site was determined using a purposive sampling method. Using the sediment core, sediment samples were taken three times in each location. The levels of Pb and Cd metals in sediment samples were analysed using an Atomic Absorption Spectrophotometer (AAS) at the Semarang BBTPPI Laboratory. The analysis of the metal content of Pb in the sediment showed that Tirang Beach had the highest concentration of heavy metal at 49.16 mg/kg, and Trimulyo Beach had the highest Pb content at 23.78 mg/kg. The results showed that the Cd metal content in sediments at Trimulyo Beach and Tirang Beach was >0.050 mg/kg. Environmental parameters measured in situ (temperature, salinity, pH, DO, current velocity, and water clarity) made a contribution to the heavy metal content in the sediment. Overall, the heavy metal content of Cd in the sediment at each research location did not exceed the quality standard, while the lead heavy metal exceeded or approached the quality standard.
Isolasi dan Karakterisasi Bakteri Asosiasi Daun Cymodocea serrulata di Perairan Pulau Panjang, Jepara Setiyorini, Alin; Pringgenies, Delianis; Ridlo, Ali
Journal of Marine Research Vol 12, No 4 (2023): Journal of Marine Research
Publisher : Departemen Ilmu Kelautan, Fakultas PerikanJurusan Ilmu Kelautan, Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jmr.v12i4.38169

Abstract

Lamun merupakan jenis tumbuhan berbunga yang mampu hidup terendam di laut dan memiliki banyak manfaat. Lamun memiliki banyak peran penting bagi ekossitem laut salah satu contohnya adalah sebagai sediment trap dan juga pemfiksasi CO2. Lamun juga memiliki fungsi penting lain bagi biota yang hidup di ekosistem lamun, mulai dari tempat berlindung dari predator, spawning dan feeding ground. Spesies Cymodocea sp. merupakan salah satu genus lamun yang dapat ditemukan di Pulau Panjang, Jepara. Lamun memiliki organisme asosiasi, salah satunya bakteri asosiasi yang memegang peranan penting secara biologi dan ekologis antar interaksi tumbuhan lamun dengan lingkungannya. Penelitian ini bertujuan untuk mengetahui spesies lamun yang digunakan dan mengetahui adanya bakteri asosiasi pada daun lamun tersebut. spesies lamun diidentifikasi secara morfologi sesuai buku panduan identifikasi LIPI. Bakteri asosiasi lamun diisolasi dari daun lamun dengan menggunakan metode pengenceran berseri. Isolat bakteri tersebut dikarakterisasi secara morfologi untuk mendapatkan isolat bakteri dengan ciri morfologi yang berbeda. Lamun dan bakteri asosiasi dapat menghasilkan senyawa bioaktif yang mirip antara keduanya sehingga dapat dimanfaatkan dalam bidang bioteknologi dan farmasi laut. Hasil penelitian menunjukkan bahwa spesies lamun yang digunakan adalah jenis Cymodocea serrulata. Hasil penelitian juga menunjukkan bahwa terdapat 7 isolat bakteri asosiasi daun lamun yang berhasil diisolasi dan memiliki karakteristik morfologi yang berbeda. Keberadaan bakteri asosiasi pada daun lamun ini memberikan informasi tentang terjadinya hubungan interaksi antara bakteri dan tumbuhan lamun.   Seagrass is a type of flowering plant that can live submerged in the sea and has many benefits. Seagrass has many important roles for marine ecosystems, one example is as a sediment trap and also a CO2 fixer. Seagrasses also have other important functions for biota that live in seagrass ecosystems, starting from shelter from predators, spawning and feeding ground. Species Cymodocea sp. is one of the seagrass genera that can be found on Panjang Island, Jepara. Seagrass has associated organisms, one of which is associated bacteria which play an important role biologically and ecologically in the interaction between seagrass plants and their environment. This study aims to determine the species of seagrass used and to determine the presence of associated bacteria in the seagrass leaves. Seagrass species were identified morphologically according to the LIPI identification guide. Seagrass-associated bacteria were isolated from seagrass leaves using serial dilution method. The bacterial isolates were characterized morphologically to obtain bacterial isolates with different morphological characteristics. Seagrasses and associated bacteria can produce bioactive compounds that are similar between the two so that they can be used in marine biotechnology and pharmaceuticals. The results showed that the seagrass species used was Cymodocea serrulata. The results also showed that there were 7 bacterial isolates associated with seagrass leaves which had different morphological characteristics. The presence of associated bacteria on seagrass leaves provides information about the occurrence of an interaction relationship between bacteria and seagrass plants.
Potential of Sea Cucumbers as Fuctional Foods Wodi, Stevy Imelda Murniati; Dewi, Eko Nurcahya; Riyadi, Putut Har; Pringgenies, Delianis; Dolorosa, Roger G.
ILMU KELAUTAN: Indonesian Journal of Marine Sciences Vol 29, No 1 (2024): Ilmu Kelautan
Publisher : Marine Science Department Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ik.ijms.29.1.97-103

Abstract

Sea cucumbers have great potential for managing water resources because they have filter feeder properties that can clean water from harmful substances. Apart from that, sea cucumbers also contain active compounds which have properties as functional foods and can help prevent cancer. Samples of sea cucumbers were collected from Karimunjawa, Central Java, and Surabaya. The research included tests for bacteria, chemicals, and heavy metals on dried sea cucumbers, as well as amino acid analysis. Two species of sea cucumbers were identified: Holothuria atra and Stichopus variegates. H. atra had a higher amino acid content and the highest collagen content among all the amino acids (14,600mg.100g-1). Both types of sea cucumber samples contain amino acids that have anti-inflammatory, antioxidant, and anticancer properties. This is due to their content of saponins, tannins, flavonoids, steroids, and terpenoids, which are beneficial for health. Additionally, S. variegates has been found to have antibacterial activity against Staphylococcus aureus and Escherichia coli bacteria. The amino acid analysis of S. variegates shows the presence of sulfate and alanine compounds, which are not found in H. atra. Research has shown that sea cucumbers can be consumed as supplements or additional food to support health and prevent various diseases, including cancer. The use of sea cucumbers as functional food ingredients also has the potential to manage water resources sustainably. In conclusion, H. atra and S. varigatus sea cucumbers have great potential for managing water resources for functional food and cancer prevention efforts. Their high content of amino acids and other phytochemical compounds makes them a good choice for development as functional food ingredients.
The Diversity of Molluscs (Gastropods and Bivalves) in Wabula Waters, Buton Regency, Southeast Sulawesi: Indonesia Haydar, Haydar Ali Yahya; Pringgenies, Delianis; Wijayanti, Diah P.
Jurnal Moluska Indonesia Vol. 8 No. 2 (2024): Oktober 2024
Publisher : Masyarakat Moluska Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.54115/jmi.v8i2.102

Abstract

Gatropods and Bivalvia class mollusks can be found in both freshwater and marine environments. Mollusks from the Gastropod and Bivalvia classes that live in the sea are often found in the intertidal to subtidal zones. Wabula is one of the coastal areas on Buton Island. The waters of Wabula are used as a tourism site, traditional site, and also as a conservation area for fisheries resources. Information regarding the diversity of mollusks in Wabula waters is still limited. This research aims to determine the diversity of mollusk species in the Gastropod and Bivalvia classes in Wabula waters through the analysis of density, diversity, and dominance indices. This research uses quadrant transects measuring 1 x 1 meters. The research was carried out at three stations: Station I on the coast, Station II in seagrass beds, and Station III in the coral reef ecosystem. The composition of the mollusks from the Gastropod and Bivalvia classes found was 158 individuals, consisting of 20 species from the Gastropod class and 3 species from the Bivalvia class. The most commonly found mollusk genera are Rhinoclavis vertagus and Nerita histrio. The ecological index values ​​obtained are density ranging from 10.3-28 Indiv/; diversity (H') 1.44-1.77; uniformity (E) 0.73-0.90; and dominant (D) 0.19-0.34.
Determinasi Teripang Dari Sisi Perairan Kepulauan Sangihe, Sulawesi Utara Wodi, Stevy Imelda Murniati; Dewi, Eko Nurcahya; Riyadi, Putut Har; Pringgenies, Delianis
Jurnal Kelautan Tropis Vol 27, No 3 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i3.23544

Abstract

This research focused on determination of sea cucumber in Sangihe Island, North Sulawesi. This study aims to explore species diversity of sea cucumber. Samples were collected with scuba diving and underwater camera, for morphological and spicule identification. This research found found 5 species in Sangihe Island, are Personothuria graffei, Bohadchia argus, Stichopus horrens, Holothuria fuscogilva, and Actinopyga miliaris. Pearsonothuria graffei had silk-likely colour, and Bohadchia argus with a dotted pattern around the body. Meanwhile, Stichopus horrens, Holothuria fuscogilva, and Actinopyga miliaris had a difference in body shape and the colour. This research significantly revealed the species diversity of sea cucumber for the sustainable resources along with the protection of the environmental condition in Sangihe Island waters, North Sulawesi. The information can be utilized as a step to protect and preserve the existence of sea cucumbers, which are a crucial component of the marine ecosystem. Penelitian tentang teripang di sisi perairan kepulauan Sangihe, Sulawesi Utara merupakan studi yang bertujuan untuk mengetahui jenis-jenis teripang yang hidup di daerah tersebut. Metode yang digunakan adalah pengumpulan sampel menggunakan scuba diving dan kamera bawah air. Penggunaan scuba diving memungkinkan untuk mengumpulkan sampel sehingga memungkinkan juga ambil foto teripang dibawah air dengan habitatnya. Hasil dari penelitian ini menunjukkan adanya lima jenis teripang yang ditemukan, yaitu Pearsonothuria graeffei (Sutra), Bohadchia argus (Bintik/Batik), Stichopus horrens, Holothuria fuscogilva, dan Actinopyga miliaris. Pearsonothuria graeffei adalah teripang yang memiliki ciri khas berwarna seperti sutra, Bohadchia argus memiliki bintik atau motif batik pada tubuhnya, sedangkan Stichopus horrens, Holothuria fuscogilva, dan Actinopyga miliaris memiliki perbedaan dalam bentuk dan warna tubuhnya. Penelitian ini dapat memberikan informasi penting tentang diversitas teripang di sisi perairan kepulauan Sangihe, Sulawesi Utara. Koleksi sampel yang dilakukan juga dapat memberikan gambaran tentang kondisi ekosistem perairan tersebut. Informasi ini dapat digunakan sebagai langkah untuk melindungi dan mempertahankan keberadaan teripang yang menjadi bagian penting dari ekosistem laut. 
Potensi Antioksidan dan Karakterisasi Pada Kolagen Teripang (Stichopus horrens) Suryani, Askiya Intan; Pringgenies, Delianis; Setyati, Wilis Ari
Jurnal Kelautan Tropis Vol 27, No 3 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i3.23892

Abstract

Stichopus horrens, a marine organism known for its numerous health benefits, particularly in the medical field, was the focus of this study, which aimed to evaluate the collagen characterization and antioxidant potential of this species. An exploratory descriptive method was employed. The S. horrens samples were extracted using NaOH, CH3COOH, and distilled water to obtain collagen. After freeze-drying, the total collagen yield was 39.3%. FTIR analysis revealed the highest collagen peak at Amide A (3280.89 cm⁻¹), indicating O-H stretching. Amino acid analysis using HPLC showed L-Leucine as the highest essential amino acid (10.6%), while L-Methionine was the lowest (0.1%). Among non-essential amino acids, L-Glutamic acid was the highest (25.7%), with L-Cystine as the lowest (0.5%). Antioxidant activity was evaluated using DPPH, ABTS, and FRAP assays. The IC50 values were 91.12 ppm for DPPH and 117.17 ppm for ABTS. The FRAP assay indicated a Trolox equivalent value of 153.90 mmol/g. Antioxidant evaluation via DPPH and ABTS assays showed moderate antioxidant strength, while the FRAP assay indicated strong antioxidant potential. Teripang Stichopus horrens, organisme laut yang memiliki berbagai manfaat kesehatan, khususnya di bidang medis. Penelitian ini bertujuan untuk mengevaluasi karakterisasi kolagen dan potensi antioksidan S. horrens. Metode eksploratif deskriptif digunakan. Sampel S. horrens diekstraksi menggunakan NaOH, CH3COOH, dan air suling untuk mendapatkan kolagen. Total hasil kolagen setelah pengeringan beku adalah 39,3%. Analisis FTIR menunjukkan puncak kolagen tertinggi pada Amida A 3280,89, yang menunjukkan peregangan OH. Analisis asam amino menggunakan HPLC mengungkapkan L-Leusin sebagai asam amino esensial tertinggi (10,6%) dan L-Metionin sebagai yang terendah (0,1%). Di antara asam amino nonesensial, L-Asam Glutamat adalah yang tertinggi (25,7%), dan L-Sistin terendah (0,5%). Aktivitas antioksidan dinilai menggunakan metode DPPH, ABTS, dan FRAP, dengan nilai IC50 sebesar 91,12 ppm untuk DPPH dan 117,17 ppm untuk ABTS. Uji FRAP menunjukkan nilai ekuivalen Trolox sebesar 153,90 mmol/g. Uji antioksidan dengan metode DPPH dan ABTS menunjukan kekutan antioksidan sedang dan untuk FRAP dalam kategori yang kuat.
Kluster Gen Biosintetik (NRPS/PKS) Pada Bakteri Sedimen Mangrove Pantai Tirang Semarang Indonesia Widayat, Barra Muzaffar; Pringgenies, Delianis; Setyati, Wilis Ari
Jurnal Kelautan Tropis Vol 27, No 3 (2024): JURNAL KELAUTAN TROPIS
Publisher : Universitas Diponegoro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jkt.v27i3.22317

Abstract

This study aims to explore biosynthetic gene clusters and antibacterial activity in mangrove sediment bacteria at Tirang Beach, Semarang, Central Java. Research methods include isolating bacteria from seagrass sediments, antibacterial testing against S. aureus and E. coli bacteria, identifying bacteria using the DNA method, and testing the presence of biosynthetic gene clusters. The results showed that the mangrove sediment bacterial isolate with the isolate code B.26.ST.3.4 had the highest antibacterial activity with a value of 28.05 ± 0.9192 against the pathogen E. coli and 23.45 ± 10.2530 against the pathogen S. aureus. Apart from that, there are two other isolates that also have antibacterial activity. Based on bacterial DNA identification, mangrove sediment bacteria that have potential as antibacterials are Bacillus velezensis (B.9.ST.1.4), Bacillus subtilis (B.13.ST.2.2), and Bacillus amyloliquefaciens (B.26.ST.3.4). These three isolates have the NRPS gene, and isolate B.9.ST.1.4 also has the PKS-II gene, while isolate B.26.ST.3.4 has the PKS-I gene. This discovery provides a deeper understanding of the antibacterial potential of mangrove sediment bacteria at Tirang Beach, Semarang. This information can be used to develop natural antibiotics that are more effective and have the potential to fight bacterial infections that are increasingly resistant to conventional antibiotics. The conclusion is that bacteria have potential as antibacterials from mangrove sediment bacteria at Tirang Beach, Semarang through isolation and identification of bacteria. Isolate B.26.ST.3.4 has the highest antibacterial activity against E. coli and S. aureus pathogens. Apart from that, Bacillus velezensis, Bacillus subtilis, and Bacillus amyloliquefaciens also have antibacterial potential. The NRPS gene was found in all three isolates, while isolate B.9.ST.1.4 also had the PKS-II gene and isolate B.26.ST.3.4 had the PKS-I gene. This discovery can provide a deeper understanding of the natural antibiotic potential of mangrove sediment bacteria and can be the basis for further development in the pharmaceutical field.  Penelitian ini bertujuan untuk mengeksplorasi klaster gen biosintesis dan aktivitas antibakteri pada bakteri sedimen mangrove di Pantai Tirang, Semarang, Jawa Tengah. Metode penelitian meliputi isolasi bakteri dari sedimen lamun, uji antibakteri terhadap bakteri S. aureus dan E. coli, identifikasi bakteri dengan metode DNA, dan uji keberadaan klaster gen biosintesis. Hasil penelitian menunjukkan bahwa isolat bakteri sedimen mangrove dengan kode isolat B.26.ST.3.4 memiliki aktivitas antibakteri tertinggi dengan nilai 28,05±0,9192 terhadap patogen E. coli dan 23,45±10,2530 terhadap patogen S. aureus. Selain itu, terdapat dua isolat lain yang juga memiliki aktivitas antibakteri Berdasarkan identifikasi bakteri DNA, bakteri sedimen mangrove yang potensial sebagai antibakteri adalah Bacillus velezensis (B.9.ST.1.4), Bacillus subtilis (B.13.ST.2.2), dan Bacillus amyloliquefaciens (B.26.ST.3.4). Ketiga isolat ini memiliki gen NRPS, dan isolat B.9.ST.1.4 juga memiliki gen PKS-II, sedangkan isolat B.26.ST.3.4 memiliki gen PKS-I. Penemuan ini memberikan pemahaman yang lebih dalam tentang potensi antibakteri dari bakteri sedimen mangrove di Pantai Tirang Semarang. Informasi ini dapat digunakan untuk mengembangkan antibiotik alami yang lebih efektif dan memiliki potensi dalam melawan infeksi bakteri yang semakin resisten terhadap antibiotik konvensional. Kesimpulan bahwa bakteri potensi sebagai antibakteri dari bakteri sedimen mangrove di Pantai Tirang, Semarang melalui isolasi dan identifikasi bakteri. Isolat B.26.ST.3.4 memiliki aktivitas antibakteri tertinggi terhadap patogen E. coli dan S. aureus. Selain itu, Bacillus velezensis, Bacillus subtilis, dan Bacillus amyloliquefaciens juga memiliki potensi sebagai antibakteri. Gen NRPS ditemukan pada ketiga isolat tersebut, sedangkan isolat B.9.ST.1.4 juga memiliki gen PKS-II dan isolat B.26.ST.3.4 memiliki gen PKS-I. Penemuan ini dapat memberikan pemahaman yang lebih dalam tentang potensi antibiotik alami dari bakteri sedimen mangrove dan dapat menjadi dasar untuk pengembangan lebih lanjut dalam bidang farmasi.
Antibacterial, Antifungal, Antioxidant, and Photoprotective Analysis of Mangrove Extracts as Additives Ingredients in a Cosmetic Cream Putri, Dear Berliana; Pringgenies, Delianis; Trianto, Agus
HAYATI Journal of Biosciences Vol. 32 No. 3 (2025): May 2025
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.32.3.571-580

Abstract

Mangroves are a rich source of natural antioxidant compounds that can inhibit and reduce tissue damage caused by free radical activity. Currently, cosmetic creams are widely recommended for maintaining skin health and aesthetics, especially in protecting against the harmful effects of free radicals. This study applied mangrove extract as an additive ingredient in a cosmetic cream, serving as an antibacterial, antifungal, antioxidant, and photoprotective agent. Mangrove samples were collected from the mangrove ecosystem in Tapak Village, Semarang, Indonesia. Three mangrove species were evaluated for their bioactivity, and the species with the highest bioactivity was selected for use in the cream formulation. Antibacterial and antifungal activities were tested using the disc diffusion method, antioxidant activity was assessed using the DPPH method, and photoprotective activity was determined using UV spectrophotometry, with analysis based on the Mansur mathematical equation. The cream was evaluated for its characteristics, including spreadability, homogeneity, sensory properties, pH, sun protection factor (SPF), phytochemical content, stability, and microbial contamination. An in vivo was conducted to assess the cream’s effectiveness on white mice. Results indicated that Avicennia marina leaf extract exhibited the highest bioactivity compared to the other two species, Rhizophora mucronata and Bruguiera gymnorrhiza. Consequently, A. marina leaf extract was selected as the active additive ingredient in the cream formulation. Characterization tests demonstrated that the cream was stable and met standard criteria for quality. The in vivo analysis revealed that the mangrove leaf extract cream significantly prevented epidermal thinning, reduced neutrophil counts, and preserved fibroblast numbers.
Co-Authors Adi Santoso Adityo Harmawan Adityo Harmawan Agung Sudaryono Agus Hartoko Agus Sabdono Agus Trianto Agus Trianto Aiyen Tjoa Alfi Satriadi Alfin Anggraeni Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Djunaedi Ali Ridho Ali Ridho Ali Ridho Ali Ridlo Ambariyanto Ambariyanto Amelia C. Anggelina Angelina Ferawaty Siregar Angelina Ferawaty Siregar Angelina, Amelia Cahya Anggelina, Amelia Cahya Anggi Setiabudi Aninditia Sabdaningsih Aninditia Sabdaningsih Anita Dwi Kartika Annisa Rhamadany Anuwat Nateewathana Ari Setyati , Wilis Arief Dwi Kurniawan Ariyanto, Dafit Ariyanto Avigail, Yolanda Bunjamin Dharma Chrisna Adhi Suryono Dafit Ariyanto Dafit Ariyanto Dewi, Lutfianna Fatma Diah Ayu Mustofa Diah Tri OktaviyantI Dinda Richa Kumarahaqi Dinny Anjang Sari Dolorosa, Roger G. Dony Bayu Putra Pamungkas Dwi Haryanti, Dwi Dwicahyo Setiyo Wibowo Edi Setiyono Eko Nurcahya Dewi Endah Sari Endang Sri Susilo Endang Sri Susilo Ervia Yudiati Ervia Yudiati Esti Rudiana Eunike Noviana Pranoto Evi Lutfiyani Fadhliyah Idris Fahmi Rizal Gaol, Josua Gabriel Lumban Girsang, Putri Hutari Gunawan Widi Santosa Gunawan Widi Santosa Hadi Endrawati Haeruddin Haeruddin Haeruddin Haeruddin Hanif, Marwa Irfan Haydar, Haydar Ali Yahya Hendrayana Hendrayana Hilda Ayu Aprilia Hilda Ayu Aprilia Idris, Riyada Iin Putriyani Indrawan , Ahmad Faras Irwani Irwani Ita Riniatsih Jelita Rahma Hidayati Kartika Dewi Koichiro Awai Lumban Gaol, Josua Gabriel Lutfianna Fatma Dewi Ma'ruf, Widodo Farid Masnah Jumiati Maulina, Dinda Rizky Ayu Mijil Ciptaning Dananjoyo Monika, Rika Muhammad Fatoni Muhammad S. Bahry Muhammad Syaifudien Bahry Muhammad Zainuddin Muhammad Zainuddin Muhammad Zainuddin Mujiyanto Mujiyanto Mukti K. Diana Putri Mukti K. Diana Putri Mustofa, Diah Ayu Nerva Sembiring Ni'mah, Ulin Nur Taufiq Nur Taufiq S.P.J. Nur Taufiq Syamsudin Putra Jaya Taufiq Syamsudin Putra Jaya Nuraini , Ria Azizah Tri Nurul Fitrah Hasanah Nurul Fitrah Hasanah Nuskiya, Amila Ocky Karna Radjasa Ocky Karna Radjasa Ocky Karna Radjasa Pasaribu, Roberto Patar Person Pesona Renta Pranoto, Aris Kabul Putri Hutari Girsang Putri, Dear Berliana Putut Har Riyadi Retno Hartati Rhamadany, Annisa Ria Azizah Ria Azizah T.N. Ria Azizah Tri Nuraeni Ria Azizah Tri Nuraini Ribka Anindita Br Perangin-angin Richardus Mahatmada Indrajati Rignolda Djamaludin Rika Monika Rini Pramesti Rini Widiyadmi Rini Widyadmi Rudhi Pribadi Ryandha Idris Sakti Imam Muchlissin Salsabilla, Sauwa Khusna Sari Budi Moria Sarif, Fina Saffuteri Setiyorini, Alin Setyati , Wilis Ari Sibero, Mada T Siti Aisyah Siti Rudiyanti Sri Lintang Artono Sri Redjeki Sri Redjeki Sri Sejati Sri Yulina Wulandari Stevy Imelda Murniati Wodi Subagiyo Subagiyo Suryani, Askiya Intan Tatas H.P. Brotosudarmo Tedi Septiadi Tri Adi Nugroho Ucu Yanu Arbi Ulfah Rahmayani Ulin Ni'mah Vicencius Hendra Adhari Vincent Theodorus Siringoringo Vivi Endar Herawati Wahyudi, Dimas Waluyo, Anasri Widayat, Barra Muzaffar Widianingsih Widianingsih Wijaya, Patria Ami Wijayanti, Diah P. Wilis Ari Setyati Wilis Ari Setyati Yolanda Avigail Zaenal Arifin Zaenal Arifin Zaneb M. Ben Mansur