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All Journal Jurnal Sain Veteriner Jurnal Veteriner Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture Science Journal of Aquaculture and Fish Health Journal of Parasite Science Journal of Marine and Coastal Science Journal of Fisheries & Marine

Kusnoto Kusnoto

Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

Author-ID : 3558585

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

Published : 36 Documents Claim Missing Document

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The Distribution of Goat Gastrointestinal Tractus Worm Egg at Rambon District of Nganjuk Regency Arum Puspitasari; Boedi Setiawan; Setiawan Koesdarto; Kusnoto Kusnoto; Soeharsono Soeharsono; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the distribution of worm tract infection in foat digestion in Sub-district of Prambon, Nganjuk Regency. This research was conducted in Agustus 2018-Januari 2019 with 108 samples of stool examination iin laboratory of Helmintologi Airlangga University Department of Parasitology, were examinated by native, sedimentation. Floatation techniques, and distribution of worm types of feces. The results showed that 66 samples were positive infected by gastrointestinal worm, its indicated that helminthiasis was 61,11%. On examination it was found some kind of worm eggs, which are: Oesophagustomum spp., Bunostomum spp., Haemonchus spp., Trichostrongylus spp., Strongyloides spp., Trichuris spp., Chabertia spp and Moniezia benedini. The results of study showed the most of worm distribution in feces is Haemonchus spp.

Heminthiasis in a Wild Rats (Rattus sp.) in Surabaya Hemasayu Nirmala Putri; Budiarto Budiarto; Arimbi Arimbi; Lucia Tri Suwanti; Kusnoto Kusnoto; Soeharsono Soeharsono
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The research was done to investigated and find out whether in the rat’s (Rattus sp.) body in Surabaya contained zoonotic helminth.This research used 31 rats taken from North Surabaya, South Surabaya, East Surabaya, West Surabaya and Central Surabaya, regardless of gender and age. 1 sample from Mulyosari, 3 samples from Bratang, 7 samples from Pegirian, and 20 samples came from Keputran. The result of 31 samples of rat’s digestive organs examine using a surgical method showed positive resulth of the presence of Hymenolepis diminutain the intestine and 5 positive samples were Capillaria hepatica, and then 25 positive samples were Taenia taeniaformis obtained from rat liver organs. From exploration using a microscope, 27 samples showed positive result in the presence of Capillaria hepatica’s eggs. Helminth found in this research are Hymenolepis diminuta, Taenia taeniaformis. and Capillaria hepatica which are zoonotic.

Prevalence of Rumen and Reticulum Trematodes in The Goat Slaughtered at Pegirian Surabaya Slaughter House using Digestive Surgery Method Novia Intan Kurnia; Setiawan Koesdarto; Herry Agoes Hermadi; Kusnoto Kusnoto; Hardany Primarizky; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the kind of trematodes which infected the rumen and reticulum of goat slaughtered at RPH Pegirian Surabaya using digestive surgery method, and determine the prevalence result. 36 samples of rumen and reticulum were collected from RPH Pegirian from September - November 2017 then using digestive surgery to obtain fresh adult worms, afterwards using the Semichen-Acetic Carmine staining method to makes the object easy to identifying. The results showed that species was found on goat was Paramphistomum cervi and Cotylophoron cotylophorum, in the Paramphistomum cervi are several characters wich is oral sucker, genital pore, uterus, testis, ventral sucker and in Cotylophoron cotylophorum contain oral sucker, branched oesophagus, uterus, vitelin glands, testes , posterior sucker. 3 rumen positive trematodosis with prevalence of 8.33% Chi-square statistic test (p>0.05) showed no effect between goat species and Trematodosis prevalence, 20 kacang goat and 16 peranakan etawa goat found 2 positive kacang goat (10%) and 1 peranakan etawa goat positive (6.25%).

Prevalence and Identification of Digestive Tract Protozoa in Goats in Labang District Bangkalan Regency Warda Nafalizza Efendi; Lucia Tri Suwanti; Abdul Samik; Poedji Hastutiek; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the prevalence and to identify gastrointestinal protozoa in goats in Sub-district of Labang, Bangkalan Regency, Madura, Indonesia. The research was conducted in April - July 2019 with 100 samples of goat’s stool. Examination was done in the laboratory of Parasitology in Faculty of Veterinary Medicine, Airlangga University using direct smear, sedimentation and flotation methods. The result showed that goats in Labang, Bangkalan Regency were infected by protozoa Eimeria sp. and Blastocystis sp. and the prevalence was 84%. There were no significant differences in the age and sex of goats (p > 0.05).

Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Firman Hadi Fanani; Kusnoto Kusnoto; Poedji Hastutiek; Setiawan Koesdarto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 Î¼g/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.

Gambaran Jumlah dan Hitung Jenis Leukosit Ayam Petelur yang diinfeksi L2 Toxocara Cati Diyah Ayu Candra; Nunuk Dyah Retno Lastuti; Nove Hidayati; Kusnoto Kusnoto; Poedji Hastutiek; Retno Bijanti
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this study was to determine leukocytes and differential counting in chicken after infected with L2 Toxocra cati. In this study, it was used twenty chickens, wich were 14 weeks old. They were divided into 4 groups. Chickens were infected by orally with dose 0 eggs/ml L2 T. cati, 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. Blood sampling were conducted on 2, 7 and 21 days after infection. Leukocyte value was determined by improve neubauer and differential counting stained with Wright’s stain then determined using microscope 1000x. The data was analyzed by Anova Factorial and then continued by BNJ Test at 5%. The result showed that increased of leukocytes value and eosinophil at 7 and 21 days after infection L2 T. cati with different dose, increased of leukocytes value and eosinophil on infection dose 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. It shows that there was not intereraction between time of infection process and infection dose of L2 T. Cati.

Identifikasi Cacing Nematoda pada Sekum dan Kolon Sapi Kurban yang Dipotong saat Idul Adha 1439 H di Wilayah Surabaya Timur Jihaan Haajidah; Moh Sukmanadi; Kusnoto Kusnoto; Endang Suprihati; Lianny Nangoi; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

The aim of this research was to detect the presence of nematode worms that infects sacrificial cattle slaughtered duringEid al-Adha 1439 H in East Surabaya. This research used 24 sampels of sacrificial cattle and used digestive tract surgical method. Based on the result of the examination using a microscope with 100x magnification, there are positive samples infected with nematode worms. In caecum cattle consist of Oesophagostomum radiatum and Trichuris spp. In colon cattle consist of Oesophagostomum radiatum.

Identifikasi Parasit Pentastomida pada Biawak Air (Varanus salvator) yang akan dikonsumsi Andhika Yudhantama Subroto; I Komang Wiarsa Sardjana; Moh Sukmanadi; E Djoko Poetranto; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

This study aims to determine the pentastomide parasite that infects the digestive tract of giant water lizards examined in Laboratory of Parasitology Department, Faculty of Veterinary Medicine, Airlangga University. In this study, three sample were found to be infected with pentastomide parasite in the lung. The phase found was adult phase pentastomide; it has pentastomide spermatheca which shows that this parasite is female.

Correlation of Water Quality Against Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in the Floating Net Cages Urban Farming Program in Surabaya, East Java Cintia Larasati; Gunanti Mahasri; Kusnoto kusnoto
Journal of Marine and Coastal Science Vol. 9 No. 1 (2020): FEBRUARY
Publisher : Universitas Airlangga

The main obstacle to the cultivation of tilapia (Oreochromis niloticus) in the KJA Urban Farming Program in Surabaya is unstable water quality. This is because the reservoirs and bozem used are waters whose water quality depends on the water source so that if the environment experiences a decrease in water quality, the activity of disease agents such as parasites will increase and the health condition of fish will decrease. This condition allows fish to be more susceptible to ectoparasite infestations. This study aims to determine the correlation of water quality with the prevalence of ectoparasites in tilapia in the KJA Urban Farming Program in Surabaya. The method used is a survey method. The results of measurements of water quality parameters indicate that the temperature ranges between 29-30C, pH 7-8.5, brightness 18-30 cm, dissolved oxygen 4.8-5.3 mg / l, nitrite <0.043-2.213 mg / l, nitrate <0.008-7.781 mg / l and ammonia 0.063-0.35 mg / l. The results of the average prevalence of Trichodina ectoparasites were 73.85% and Dactylogyrus ectoparasites were 2.85%. There is a positive correlation between temperature, pH, nitrate, nitrite with the prevalence of Trichodina, and temperature, pH, and nitrate with the prevalence of Dactylogyrus. There is a negative correlation between brightness, dissolved oxygen and ammonia with the prevalence of Trichodina, and brightness, nitrite, dissolved oxygen, and ammonia with the prevalence of Dactylogyrus.

WAKTU HENTI CHLORAMPHENICOL PADA LOBSTER (Cherax quadricarinatus) AIR TAWAR Miftahul Jannah; Hari Suprapto; Kusnoto Kusnoto
Journal of Aquaculture and Fish Health Vol. 5 No. 1 (2016): JAFH Vol. 5 No. 1 Februari 2016
Publisher : Department of Aquaculture

Permintaan yang tinggi oleh konsumen terhadap produk perikanan terutama lobster (Cherax quadricarinatus) air tawar dari tahun ketahun memacu perkembangan industri budidaya lobster yang sangat pesat. Namun dalam proses budidaya tersebut banyak sekali kendala yang muncul. Salah satu kendala yang umum dihadapi dalam budidaya ikan adalah adanya serangan penyakit yang dise- babkan oleh bakteri. Beberapa strategi pencegahan penyakit yang telah diaplikasikan dalam budida- ya lobster, salah satunya menggunakan antibiotik. Salah satu antibiotik yang sering digunakan pembu- didaya adalah Chloramphenicol. Pada Lobster, penggunaan Chloramphenicol dengan dosis tinggi akan menyebabkan resistensi terhadap bakteri patogen. Pelaksanaan penelitian di Laboratorium Pendidikan Perikanan, Fakultas Perikanan dan Ke- lautan, Universitas Airlangga dan Balai Karantina Ikan Pengendalian Mutu dan Keamana Hasil Peri- kanan Kelas I Surabaya II. Penelitian ini berlangsung pada bulan November 2013 sampai bulan Ja- nuari 2014. Tujuan penelitian ini adalah untuk mengetahui lama waktu henti obat yang diperlukanagar residu antibiotik Chloramphenicol yang terdapat pada Lobster (Cherax quadricarinatus) air tawar me- nurun hingga batas aman untuk dikonsumsi. Lobster yang diberi pakan dengan campuran chlo- ramphenicol dengan dosis 2g/kg pakan terdeteksi residu Chloramphenicol tertinggi sebanyak 31,962 ppb pada minggu pertama dan terjadi penurunan residu menjadi 3.53 ppb selama delapan minggu.

Co-Authors Abdul Samik Achmad Hasan Sahani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Andhika Yudhantama Subroto Andriani Dwi Siswarini Arimbi Arimbi Arum Puspitasari Bambang Sektiari Lukiswanto Bernideta Dewi Kriswijayanti Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Candra Dwi Atma Chusniati, Sri Cintia Larasati Dhio Asmaydo Didik Handijatno Diyah Ayu Candra E Djoko Poetranto E Djoko Poetranto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestinah Ellza Agatha Damayanti Endang Suprihati Eny Coolfina Simarmata Era Insivitawati Erma Safitri Fania Selfiannisa Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hari Suprapto Hartono Hartono Hemasayu Nirmala Putri Herry Agoes Hermadi Hervina Benazir Ardiyanti Ine Karni Iwan Sahrial Hamid Jihaan Haajidah Jola Rahmahani khoirul - Arifin Kismiyati , Koesnoto Supriandono Kuncoro Puguh Santoso Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Miftahul Jannah Mochamad Lazuardi moh. arief ma'ruf Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Ngakan Made Rai Widjaja Nisa’ Rachmaningtyas Putri Nove Hidayati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Primarizky, Hardany Rahmi Sugihartuti Ratna Damayanti Retno Bijanti Retno Bijanti Ria Nikmatul Jannah Sardjana, I Komang Wiarsa Sesa Puput Febriyanti Setiawan Koesdarto Setiawati Sigit Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sosiawati Sri Subekti Suherni Susilowati Sunarso, Agus Talita Yuanda Reksa Titom Gusmana Putra Perdana Tri Bhawono Dadi Tutik Juniastuti Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Warda Nafalizza Efendi

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