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All Journal Jurnal Sain Veteriner Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture and Fish Health Journal of Parasite Science Journal of Marine and Coastal Science Journal of Fisheries & Marine Journal of Aquaculture Science
Kusnoto Kusnoto
Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Prevalence and Identification of Digestive Tract Protozoa in Goats in Labang District Bangkalan Regency Warda Nafalizza Efendi; Lucia Tri Suwanti; Abdul Samik; Poedji Hastutiek; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (157.024 KB) | DOI: 10.20473/jops.v3i2.16527

Abstract

This study aims to determine the prevalence and to identify gastrointestinal protozoa in goats in Sub-district of Labang, Bangkalan Regency, Madura, Indonesia. The research was conducted in April - July 2019 with 100 samples of goat’s stool. Examination was done in the laboratory of Parasitology in Faculty of Veterinary Medicine, Airlangga University using direct smear, sedimentation and flotation methods. The result showed that goats in Labang, Bangkalan Regency were infected by protozoa Eimeria sp. and Blastocystis sp. and the prevalence was 84%. There were no significant differences in the age and sex of goats (p > 0.05).
Cross Reaction of Haemonchus Contortus ANTIGEN WITH ANTI-Fasciola Gigantica Serum by Using Western Blot Technique Firman Hadi Fanani; Kusnoto Kusnoto; Poedji Hastutiek; Setiawan Koesdarto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (168.508 KB) | DOI: 10.20473/jops.v4i1.20176

Abstract

The purpose of this research to know cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum by using the Western Blot technique. The result can be used as Haemonchosis serologic diagnostic. This research was conducted using Haemonchus contortus worms obtained from abomasum, especially cow worms and Fasciola gigantica worms obtained from the bile duct and gall bladder. The worms were crushed and added with Phosphate Buffer Saline (PBS) to make homogenates and then centrifuged at 3500 rpm for 15 minutes. Homogenate Fasciola gigantica was immunized to mice to get anti-Fasciola gigantica serum at a dose of 200 μg/mice and booster 3 times with a span of two weeks. Homogenates were carried out using the SDS-PAGE technique to analyze proteins using a brilliant blue dye. Cross reaction of Haemonchus contortus protein with anti-Fasciola gigantica serum byusing Western blot technique and obtained 3 protein bands are 94.9, 52.7, 46.7 kDa.
Gambaran Jumlah dan Hitung Jenis Leukosit Ayam Petelur yang diinfeksi L2 Toxocara Cati Diyah Ayu Candra; Nunuk Dyah Retno Lastuti; Nove Hidayati; Kusnoto Kusnoto; Poedji Hastutiek; Retno Bijanti
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (176.191 KB) | DOI: 10.20473/jops.v4i1.20269

Abstract

The aim of this study was to determine leukocytes and differential counting in chicken after infected with L2 Toxocra cati. In this study, it was used twenty chickens, wich were 14 weeks old. They were divided into 4 groups. Chickens were infected by orally with dose 0 eggs/ml L2 T. cati, 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. Blood sampling were conducted on 2, 7 and 21 days after infection. Leukocyte value was determined by improve neubauer and differential counting stained with Wright’s stain then determined using microscope 1000x. The data was analyzed by Anova Factorial and then continued by BNJ Test at 5%. The result showed that increased of leukocytes value and eosinophil at 7 and 21 days after infection L2 T. cati with different dose, increased of leukocytes value and eosinophil on infection dose 10 eggs/ml L2 T. cati, 100 eggs/ml L2 T. cati and 1000 eggs/ml L2 T. cati. It shows that there was not intereraction between time of infection process and infection dose of L2 T. Cati.
Identifikasi Cacing Nematoda pada Sekum dan Kolon Sapi Kurban yang Dipotong saat Idul Adha 1439 H di Wilayah Surabaya Timur Jihaan Haajidah; Moh Sukmanadi; Kusnoto Kusnoto; Endang Suprihati; Lianny Nangoi; Poedji Hastutiek
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (180.551 KB) | DOI: 10.20473/jops.v4i1.20272

Abstract

The aim of this research was to detect the presence of nematode worms that infects sacrificial cattle slaughtered duringEid al-Adha 1439 H in East Surabaya. This research used 24 sampels of sacrificial cattle and used digestive tract surgical method. Based on the result of the examination using a microscope with 100x magnification, there are positive samples infected with nematode worms. In caecum cattle consist of Oesophagostomum radiatum and Trichuris spp. In colon cattle consist of Oesophagostomum radiatum.
Identifikasi Parasit Pentastomida pada Biawak Air (Varanus salvator) yang akan dikonsumsi Andhika Yudhantama Subroto; I Komang Wiarsa Sardjana; Moh Sukmanadi; E Djoko Poetranto; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 4 No. 1 (2020): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (288.512 KB) | DOI: 10.20473/jops.v4i1.20276

Abstract

This study aims to determine the pentastomide parasite that infects the digestive tract of giant water lizards examined in Laboratory of Parasitology Department, Faculty of Veterinary Medicine, Airlangga University. In this study, three sample were found to be infected with pentastomide parasite in the lung. The phase found was adult phase pentastomide; it has pentastomide spermatheca which shows that this parasite is female. 
Correlation of Water Quality Against Prevalence of Ectoparasites in Tilapia (Oreochromis niloticus) in the Floating Net Cages Urban Farming Program in Surabaya, East Java Cintia Larasati; Gunanti Mahasri; Kusnoto kusnoto
Journal of Marine and Coastal Science Vol. 9 No. 1 (2020): FEBRUARY
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (112.164 KB) | DOI: 10.20473/jmcs.v9i1.20756

Abstract

The main obstacle to the cultivation of tilapia (Oreochromis niloticus) in the KJA Urban Farming Program in Surabaya is unstable water quality. This is because the reservoirs and bozem used are waters whose water quality depends on the water source so that if the environment experiences a decrease in water quality, the activity of disease agents such as parasites will increase and the health condition of fish will decrease. This condition allows fish to be more susceptible to ectoparasite infestations. This study aims to determine the correlation of water quality with the prevalence of ectoparasites in tilapia in the KJA Urban Farming Program in Surabaya. The method used is a survey method. The results of measurements of water quality parameters indicate that the temperature ranges between 29-30C, pH 7-8.5, brightness 18-30 cm, dissolved oxygen 4.8-5.3 mg / l, nitrite <0.043-2.213 mg / l, nitrate <0.008-7.781 mg / l and ammonia 0.063-0.35 mg / l. The results of the average prevalence of Trichodina ectoparasites were 73.85% and Dactylogyrus ectoparasites were 2.85%. There is a positive correlation between temperature, pH, nitrate, nitrite with the prevalence of Trichodina, and temperature, pH, and nitrate with the prevalence of Dactylogyrus. There is a negative correlation between brightness, dissolved oxygen and ammonia with the prevalence of Trichodina, and brightness, nitrite, dissolved oxygen, and ammonia with the prevalence of Dactylogyrus.
WAKTU HENTI CHLORAMPHENICOL PADA LOBSTER (Cherax quadricarinatus) AIR TAWAR Miftahul Jannah; Hari Suprapto; Kusnoto Kusnoto
Journal of Aquaculture and Fish Health Vol. 5 No. 1 (2016): JAFH Vol. 5 No. 1 Februari 2016
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (119.319 KB) | DOI: 10.20473/jafh.v5i1.11319

Abstract

Permintaan yang tinggi oleh konsumen terhadap produk perikanan terutama lobster (Cherax quadricarinatus) air tawar dari tahun ketahun memacu perkembangan industri budidaya lobster yang sangat pesat. Namun dalam proses budidaya tersebut banyak sekali kendala yang muncul. Salah satu kendala yang umum dihadapi dalam budidaya ikan adalah adanya serangan penyakit yang dise- babkan oleh bakteri. Beberapa strategi pencegahan penyakit yang telah diaplikasikan dalam budida- ya lobster, salah satunya menggunakan antibiotik. Salah satu antibiotik yang sering digunakan pembu- didaya adalah Chloramphenicol. Pada Lobster, penggunaan Chloramphenicol dengan dosis tinggi akan menyebabkan resistensi terhadap bakteri patogen. Pelaksanaan penelitian di Laboratorium Pendidikan Perikanan, Fakultas Perikanan dan Ke- lautan, Universitas Airlangga dan Balai Karantina Ikan Pengendalian Mutu dan Keamana Hasil Peri- kanan Kelas I Surabaya II. Penelitian ini berlangsung pada bulan November 2013 sampai bulan Ja- nuari 2014. Tujuan penelitian ini adalah untuk mengetahui lama waktu henti obat yang diperlukanagar residu antibiotik Chloramphenicol yang terdapat pada Lobster (Cherax quadricarinatus) air tawar me- nurun hingga batas aman untuk dikonsumsi. Lobster yang diberi pakan dengan campuran chlo- ramphenicol dengan dosis 2g/kg pakan terdeteksi residu Chloramphenicol tertinggi sebanyak 31,962 ppb pada minggu pertama dan terjadi penurunan residu menjadi 3.53 ppb selama delapan minggu. 
IDENTIFIKASI DAN DERAJAT INFESTASI Lernaea PADA IKAN MASKOKI (Carassius auratus) DI KABUPATEN TULUNGAGUNG, JAWA TIMUR Bernideta Dewi Kriswijayanti; Kismiyati Kismiyati; Kusnoto Kusnoto
Journal of Aquaculture and Fish Health Vol. 3 No. 1 (2014): JAFH Vol. 3 No. 1 January 2014
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.435 KB) | DOI: 10.20473/jafh.v3i1.12983

Abstract

Penyakit merupakan masalah yang penting yang harus diperhatikan oleh pembudidaya ikan agar tidak mengalami kerugian. Penyakit dapat muncul di suatu perairan akibat ketidakseimbangan antara lingkungan, inang, dan patogen. Salah satu penyakit yang sering menyerang ikan hias air tawar adalah lernaeosis. Lernaeosis adalah penyakit pada ikan yang disebabkan oleh serangan ektoparasit copepoda dari genus Lernaea. Ektoparasit ini dapat ditemukan pada seluruh permukaan tubuh, mulut dan insang ikan. Lernaea dapat menyebabkan iritasi pada kulit serta lesi. Ikan maskoki (Carrassius auratus) merupakan salah satu jenis ikan hias air tawar tergolong dalam jenis ikan karper yang sering terserang Lernaea. Ikan maskoki (Carassius auratus) merupakan komoditas unggulan perikanan budidaya untuk jenis ikan hias air tawar di Tulungagung. Identifikasi dan derajat infestasi Lernaea yang menyerang ikan maskoki (Carassius auratus) di Tulungagung, Jawa Timur belum banyak diteliti sehingga perlu dilakukan penelitian pada tiga desa yaitu Desa Karang Rejo, Desa Ploso Kandang dan Desa Wajak dimana ketiga desa tersebut merupakan sentra budidaya di Kabupaten Tulungagung. Tujuan penelitian ini adalah untuk mengetahui spesies Lernaea yang menyerang ikan maskoki (Carassius auratus) dan derajat infestasi dari serangan Lernaea pada ikan maskoki yang dibudidayakan di tiga desa yang berada di Desa Karang Rejo, Desa Ploso Kandang dan Desa Wajak, Kabupaten Tulungagung, Jawa Timur. Identifikasi Lernaea dilakukan di Laboratorium Fakultas Perikanan dan Kelautan Universitas Airlangga Surabaya dan penggambaran spesimen dilakukan di LIPI Cibinong. Data hasil identifikasi spesies Lernaea yang menyerang ikan maskoki disajikan secara deskriptif dalam bentuk gambar untuk identifikasi Lernaea dan dalam bentuk tabel untuk derajat infestasi. Hasil penelitian yang dilakukan di Desa Karang Rejo, Desa Ploso Kandang dan Desa Wajak hanya ditemukan satu spesies saja, yaitu Lernaea cyprinacea. Derajat infestasi dari serangan Lernaea cyprinacea di tiga desa tersebut tergolong dalam infestasi ringan.
Prevalence of Helminthiasis in Etawa Crossbreed Goat in Kalipuro Sub-District Banyuwangi Regency khoirul - Arifin; moh. arief ma'ruf; Kusnoto
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.851 KB) | DOI: 10.20473/jops.v6i1.34303

Abstract

Etawa crossbreed goat are meat and milk-producing livestock that can adapt to the climate in Indonesia. The barriers to raising goats include the problem of disease which is a direct influence on livestock life. This study aims to determine the prevalence of helminthiasis in Etawa Crossbreed Goat in Kalipuro District, Banyuwangi Regency. The study was conducted in January until February 2019 using 100 fecal samples. The samples examination in was performed the laboratory of Instrument Airlangga University PSDKU in Banyuwangi Prodi of Veterinary Medicine, using native, sedimentation and floatation methods. Data obtained from this study were presented descriptively and analyzed by Chi-Square test. Based on examination results, prevalence of helminthiasis in Etawa Crossbreed Goat was 43 %, which is dominately by Moniezia spp. (17%), Haemonchus spp. (15%), Trichuris spp. (9%), Strongyloides spp. (7%), Trichostrongylus spp. (7%). Furthermore, it was suggested that anthelmintic and inspection of helminthiasis in those area given periodically and continuously.  
Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi&#039; Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Abstract

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.
Co-Authors Abdul Samik Achmad Hasan Sahani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Aksono HP., Eduardus Bimo Andhika Yudhantama Subroto Andriani Dwi Siswarini Arimbi Arimbi Arum Puspitasari Bambang Sektiari Lukiswanto Bernideta Dewi Kriswijayanti Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Candra Dwi Atma Chusniati, Sri Cintia Larasati Dhio Asmaydo Didik Handijatno Diyah Ayu Candra E Djoko Poetranto E Djoko Poetranto Eka Pramyrtha Hestinah Ellza Agatha Damayanti Endang Suprihati Eny Coolfina Simarmata Era Insivitawati Erma Safitri Fania Selfiannisa Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hari Suprapto Hartono Hartono Hemasayu Nirmala Putri Herry Agoes Hermadi Hervina Benazir Ardiyanti Ine Karni Iwan Sahrial Hamid Jihaan Haajidah khoirul - Arifin Kismiyati , Koesnoto Supriandono Kuncoro Puguh Santoso Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Miftahul Jannah Mochamad Lazuardi moh. arief ma'ruf Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Rofi&#039; Prasetya Muhammad Rofi' Prasetya Ngakan Made Rai Widjaja Nisa’ Rachmaningtyas Putri Nove Hidayati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Primarizky, Hardany Rahmahani, Jola Rahmi Sugihartuti Ratna Damayanti Retno Bijanti Retno Bijanti Ria Nikmatul Jannah Sardjana, I Komang Wiarsa Sesa Puput Febriyanti Setiawan Koesdarto Setiawati Sigit Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sosiawati Sri Subekti Suherni Susilowati Sunarso, Agus Talita Yuanda Reksa Titom Gusmana Putra Perdana Tri Bhawono Dadi Tutik Juniastuti Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Warda Nafalizza Efendi