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All Journal Jurnal Sain Veteriner Jurnal Veteriner Aceh Journal of Animal Science Biosaintifika: Journal of Biology & Biology Education Journal of Aquaculture Science Journal of Aquaculture and Fish Health Journal of Parasite Science Journal of Marine and Coastal Science Journal of Fisheries & Marine

Kusnoto Kusnoto

Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

Author-ID : 3558585

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Veterinary

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Identification of Worm In Digestive Tract of Consumed Cattle Egret (Bubulcus ibis) In Ujung Pangkah, Gresik Nisa’ Rachmaningtyas Putri; E Djoko Poetranto; Nunuk Dyah Retno Lastuti; Setiawan Koesdarto; Bambang Sektiari Lukiswanto; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 2 No. 1 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

Ujung Pangkah, Gresik was became the most habitat of fowls such as Cattle egret (Bubulcus ibis). The aim of this research were to find out the type of worm that infected digestive tract of Cattle egret (Bubulcus ibis) in Ujung Pangkah, Gresik. The examination of worm in digestive tract was done by dissection and fecal examination. The identification based on morphological of the eggs and worms. The samples of digestive tract organ collected from restaurant that preserve Cattle egret as a main menu. Whereas the fecal sample collected by swab from the intestine. Cattle egret (Bubulcus ibis) has positive infection by worm parasite if there were worm or egg inside the digestive tract. Fecal samples were examined by native, sedimentation, coconut-sugar floating method and EPG counting with Lucient Brumpt. And the worm morphology from dissection of digestive tract would be identify with source book, journal, and discussed with supervisor. The result showed that 20 of digestive tract dissection and 20 fecal samples, there were three types of worm, Contracaecum sp., Cosmocephalus sp., Capillaria sp. and egg of Contracaecum sp. with 200 EPG

Investation of Ectoparasites In Chickens In Village of Kramat District of Bangkalan Regency of Bangkalan Fania Selfiannisa; Suherni Susilowati; Poedji Hastutiek; Lucia Tri Suwanti; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

A study on the infestation of ectoparasites in chicken’s raised in Village of Kramat, District of Bangkalan, Regency of Bangkalan was conducted between July to October 2017. A total of 60 chickens were examined based on their sex and region of the chicken’s body. Identification was carried out on ectoparasites found identified using permanent mounting methods and scraping. The types of ectoparasites were M. gallinae 77%, L. caponis 52%, M. stramineus 15%, R. sanguineus 3%, G. gallinae 2%, G. gigas 2%, and D. gallinae 2%. Data were analyzed using Chi Square and Correspondence. The results of infestation was higher in males than females, although the difference was not statistically significant (P>0.05). Results of analysis of the pattern of investation of ectoparasites in the region that includes in males and females body are M. gallinae in the region of the back. L. caponis in the region of the wings. M. stramineus. R. sanguineus in the region of the head-neck, G. gigas in the region of belly, G. gallinae in the region of the leg just found in the males body. D. gallinae in the region of the leg just found in the females body.

Identification of Gastrointestinal Worms Egg on Spotted Deer (Axis axis) and Bawean Deer (Axis kuhlii) at Wonorejo Bibit Park and Surabaya Flora Park Achmad Hasan Sahani; Sri Mulyati; Tri Bhawono Dadi; Sri Mumpuni Sosiawati; Kusnoto Kusnoto; Ratna Damayanti
Journal of Parasite Science (JoPS) Vol. 2 No. 2 (2018): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to identify the type of gastrointestinal worms egg of Spotted Deer (Axis axis) and Bawean Deer (Axis kuhlii) at Wonorejo Bibit Park and Surabaya Floral Park. The research was conducted in October-December 2017 used total 50 specimen of sample faeces, 40 sample faeces from Surabaya Flora Park and 10 sample faeces from Wonorejo Bibit Park, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Airlangga University. The results showed that the degree of helmint gastrointestinal in Bawean Deer and Spoted Deer at Wonorejo Bibit Park showed 0% while at the Surabaya Flora Park is 15%. The type of worm found were Trematoda class it was Fasciola sp. The resuls of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal helminth infection (p>0.05) .

Microscophy Identification of Toxocara cati First Stage Larvae and Second Stage Larvae Eny Coolfina Simarmata; Kusnoto Kusnoto; Mochamad Lazuardi; Setiawan Koesdarto; Endang Suprihati; Kuncoro Puguh Santoso
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study was aimed to identify the ultra structural morphology of Toxocara cati First Stage Larvae and Second Stage Larvae using Light Microscope. Toxocara cati larvae were obtained from adult worm eggs then were treated in phospat buffer saline with comparasion 1 : 1 until it reached the stage of larvae 1 and 2. The shell of egg Toxocara cati were thick and usually consist of three layers. The first layer was inner membrane, the second layer was middle layer and the third was outer layer. The results of larvae morphology that were identified using light microscope showed that the anterior part of Toxocara cati first stage larvae has a dorsal lip and esophagus and intestine on the posterior part but it could not be identified perfectly. The anterior part of Toxocara cati second stage larvae that were identified has three lips that leads directly into the oesophagus. Three lips on the anterior part of Toxocara cati larvae 2 consist of a dorsal lip and two subventral lips. Morphological of Toxocara cati larvae similar with the Toxocara cati adult worms because morphology of larvae and adult stage was difficult to differentiated.

The Anthelmintics Activity of Ethanol Extract of African Leaf (Vernonia amygdalina) on Mortality of Fasciola gigantica in Vitro Dhio Asmaydo; Iwan Sahrial Hamid; Kusnoto Kusnoto; Mohammad Sukmanadi; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The Aims of this study is to know the anthelmintics activity of ethanol extract of african leaf (Vernonia amygdalina) on mortality of Fasciola gigantica in Vitro. Method that used in the research was a completely randomized design. There were five treatments and each treatment was done in four replications. This research used ten Fasciola gigantica in each treatment for all replications. The observation and recording of dead Fasciola gigantica was done at 2, 4, 6, 8, and 10 hours.Â Fasciola gigantica were declared dead if there was no movement when disturbed by anatomy tweezers and when dipped in slightly warm water (500C). The obtained data was analyzed using ANOVA and continued with Duncan Multiple Range Test. The result of this research show that ethanol of extract of African Leaf (Vernonia amygdalina) has activity anthelmintic on mortality of Fasciola gigantica in Vitro. Ethanol extract african leaf (Vernonia amygdalina) with concentration of 1.4% is the optimal concentration of anthelmintics. This higher the concentration of the extract, the higher the property of anthelmintics.

Prevalence and Intensity of Nematode Infection on The Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java Sesa Puput Febriyanti; Lucia Tri Suwanti; Eka Pramyrtha Hestinah; Setiawan Koesdarto; Boedi Setiawan; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This survey study was aimed to determine the prevalance of nematodosis and intensity on the Crossbreed Horse in Detasemen Kaveleri Berkuda Parongpong Bandung West Java. The benefits of this research are to contribute giving additional information about Prevalence, Intensity, and Relation among prevalance with age, sex, and stable. Fecal samples were tested by native, sedimentation, and floatation methods. The examination was found some kind of worm eggs, which are: Trychonema sp., Strongylus spp., Dictyocaulus arnfieldi (lung worm). The result showed that the prevalence of nematodosis was 12.03%. in the calculation of worm eggs per gram feces of Crossbreed Horse in Detasemen Kaveleri Berkuda that have relatively moderate infection were 1123EPG and result analyze with ANOVA showed no real different with function of horse. The result of statistical analysis using Chi-Square test showed no significant differences in prevalence with age and sex (p>0.05). the result of statistical using Chi-Square test showed significant differences in prevalence with stable in Detasemen Kaveleri Berkuda (p<0.05).

Prevalence of Small Intestine Cestodes in Goat at Pegirian Slaughterhouse Surabaya Bryan Ahmad Affan Lubis; Setiawan Koesdarto; Eka Pramyrtha Hestinah; Kusnoto Kusnoto; Lucia Tri Suwanti
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

This study was conducted to determine the prevalence of small Intestine cestodes in goat at Pegirian Slaughterhouse Surabaya with gastrointestinal dissection method. Thirty-six samples of goatâ€™s small intestine, twenty-five from Kacang goats and eleven from Ettawa-cross (PE) goats, were collected from Pegirian Slaughterhouse Surabaya on May 2018. Fresh adult worms were collected with gastrointestinal dissection and microscopically identified. The result showed that cestodes infections could be identified in three (12%) of Kacang goats and three (27.27%) of PE goats. The cestode infections were identified five positive samples (19.23%) in male goat and one positive sample (10%) in female goat, while determined by the ages found three positive samples (17.64%) in under two years old goats and three positive samples (15.78%) in more than or equal two years old goats. The Chi-Square statistical test (p >0.05) showed there was no significant correlation from the race, sex, and ages of goats on the prevalence of Moniezia expansa.

The Prevalence and Infection’s Degree of Gastrointestinal Worm of Local Chicken (Gallus domesticus) in Kramat Village, District of Bangkalan, Madura, East Java Indonesia Ellza Agatha Damayanti; Poedji Hastutiek; Agnes Theresia Soelih Estoepangestie; Nunuk Dyah Retno Lastuti; Kusnoto Kusnoto; Endang Suprihati
Journal of Parasite Science (JoPS) Vol. 3 No. 1 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The study was aimed to know the prevalence and level of gastrointestinal worm investation of local chicken (Gallus domesticus) in Kramat Village, district of Bangkalan, Madura East Java province. The samples used in the study were 100 faeces of kampung chicken. using sedimentation and floating method. Faeces samples were tested, than followed by microscopically test to identify the worm is species. The result showed that in 38 out of 100 samples could be indentified some worm species as followed : Capillaria sp.14 (36,8%), Railletina 7 (18,4%), Hymenolepis 16 (42,1%) and Heterakis gallinarum 1 (2,6%). Using Mann Whitney U-test could be differentiated the level of the infection with an average of 45.53 in cock, while in hen with an average of 55.47. There was no significancy (p>0,05) on the level of worm gastrointestinal infection of different sex of kampung chicken.

Prevalence of Gastrointestinal Parasite on Cats in Shelter East Surabaya Akbar Wijaya Putra Purnama; Lucia Tri Suwanti; Hani Plumeriastuti; Endang Suprihati; Kusnoto Kusnoto; Agus Sunarso
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study was to determine the prevalence of gastrointesatinal parasites in cats at Surabaya shelter. Eighty two samples of cat feces used in this study were taken from Griya satwa shelter and Kiro cat shelter. Feces samples were examined using the native, sedimentation and floating methods. Feces samples gastrointestinal parasite were identified by using a microscope with 400x and 1000x magnifications. Faces samples that are diagnosed positively if there is at least one of the three methods. Feces samples has observed, obtained prevalence rate of 43.9% or 36 positive samples from eighty two samples. The results of chi square analysis showed that p > 0.05, which showed that the parasites taken in the two shelters had clear differences in the prevalence of the gastrointestinal tract parasites. The parasites observed were Isospora sp., Ancylostoma sp., Toxocara cati and Toxocara leonina.

Identification of Gastrointestinal Endoparasite in Bawean Deer (Axis kuhlii) and Spotted Deer (Axis axis) at Bratang Flora Park – Surabaya Hartono Hartono; Endang Suprihati; Erma Safitri; Nunuk Dyah Retno Lastuti; Mufasirin Mufasirin; Kusnoto Kusnoto
Journal of Parasite Science (JoPS) Vol. 3 No. 2 (2019): Journal of Parasite Science
Publisher : Universitas Airlangga

The purpose of this study is to identify the endoparasites of Spotted Bawean Deer (Axis kuhlii) and Deer (Axis axis ) at Surabaya Flora Park. The research was conducted in December 2018 - January 2019 used total 60 specimen of sample faeces, consist of 30 sample of bawean deers faeces and 30 sample of spoted deers faeces, the samples analyzed at the Department of Parasitology Veterinary, Faculty of Veterinary Medicine, Universitas Airlangga. The results of the study found positive endoparasites on examination, samples of Bawean deer faeces (Axis kuhlii) and Spotted deer faeces(Axis axis) in Baratang Flora Park Surabaya found that 26 positive samples from 60 samples were obtained. Positive endoparasitic samples are protozoa at the stage of oocyst, namely Eimeria sp. 6 (10%) samples, worm eggs from the Nematoda class, Haemonchus sp. 20 (33.33%) and whereas endoparasites from other stages and classes were not found. The results of the analysis used Chi-Square test and showed no significantly differences degree of gastrointestinal endoparasite infection of Bawean deer (Axis kuhlii) and Spotted deer (Axis axis) (p>0.05).

Co-Authors Abdul Samik Achmad Hasan Sahani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Andhika Yudhantama Subroto Andriani Dwi Siswarini Arimbi Arimbi Arum Puspitasari Bambang Sektiari Lukiswanto Bernideta Dewi Kriswijayanti Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Candra Dwi Atma Chusniati, Sri Cintia Larasati Dhio Asmaydo Didik Handijatno Diyah Ayu Candra E Djoko Poetranto E Djoko Poetranto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestinah Ellza Agatha Damayanti Endang Suprihati Eny Coolfina Simarmata Era Insivitawati Erma Safitri Fania Selfiannisa Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hari Suprapto Hartono Hartono Hemasayu Nirmala Putri Herry Agoes Hermadi Hervina Benazir Ardiyanti Ine Karni Iwan Sahrial Hamid Jihaan Haajidah Jola Rahmahani khoirul - Arifin Kismiyati , Koesnoto Supriandono Kuncoro Puguh Santoso Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Miftahul Jannah Mochamad Lazuardi moh. arief ma'ruf Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Ngakan Made Rai Widjaja Nisa’ Rachmaningtyas Putri Nove Hidayati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Primarizky, Hardany Rahmi Sugihartuti Ratna Damayanti Retno Bijanti Retno Bijanti Ria Nikmatul Jannah Sardjana, I Komang Wiarsa Sesa Puput Febriyanti Setiawan Koesdarto Setiawati Sigit Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sosiawati Sri Subekti Suherni Susilowati Sunarso, Agus Talita Yuanda Reksa Titom Gusmana Putra Perdana Tri Bhawono Dadi Tutik Juniastuti Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Warda Nafalizza Efendi

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