Kusnoto Kusnoto
Departemen Parasitologi, Fakultas Kedokteran Hewan, Universitas Airlangga, Surabaya

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IDENTIFIKASI DAN DERAJAT INFESTASI Lernaea PADA IKAN MASKOKI (Carassius auratus) DI KABUPATEN TULUNGAGUNG, JAWA TIMUR Bernideta Dewi Kriswijayanti; Kismiyati Kismiyati; Kusnoto Kusnoto
Journal of Aquaculture and Fish Health Vol. 3 No. 1 (2014): JAFH Vol. 3 No. 1 January 2014
Publisher : Department of Aquaculture

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (202.435 KB) | DOI: 10.20473/jafh.v3i1.12983

Abstract

Penyakit merupakan masalah yang penting yang harus diperhatikan oleh pembudidaya ikan agar tidak mengalami kerugian. Penyakit dapat muncul di suatu perairan akibat ketidakseimbangan antara lingkungan, inang, dan patogen. Salah satu penyakit yang sering menyerang ikan hias air tawar adalah lernaeosis. Lernaeosis adalah penyakit pada ikan yang disebabkan oleh serangan ektoparasit copepoda dari genus Lernaea. Ektoparasit ini dapat ditemukan pada seluruh permukaan tubuh, mulut dan insang ikan. Lernaea dapat menyebabkan iritasi pada kulit serta lesi. Ikan maskoki (Carrassius auratus) merupakan salah satu jenis ikan hias air tawar tergolong dalam jenis ikan karper yang sering terserang Lernaea. Ikan maskoki (Carassius auratus) merupakan komoditas unggulan perikanan budidaya untuk jenis ikan hias air tawar di Tulungagung. Identifikasi dan derajat infestasi Lernaea yang menyerang ikan maskoki (Carassius auratus) di Tulungagung, Jawa Timur belum banyak diteliti sehingga perlu dilakukan penelitian pada tiga desa yaitu Desa Karang Rejo, Desa Ploso Kandang dan Desa Wajak dimana ketiga desa tersebut merupakan sentra budidaya di Kabupaten Tulungagung. Tujuan penelitian ini adalah untuk mengetahui spesies Lernaea yang menyerang ikan maskoki (Carassius auratus) dan derajat infestasi dari serangan Lernaea pada ikan maskoki yang dibudidayakan di tiga desa yang berada di Desa Karang Rejo, Desa Ploso Kandang dan Desa Wajak, Kabupaten Tulungagung, Jawa Timur. Identifikasi Lernaea dilakukan di Laboratorium Fakultas Perikanan dan Kelautan Universitas Airlangga Surabaya dan penggambaran spesimen dilakukan di LIPI Cibinong. Data hasil identifikasi spesies Lernaea yang menyerang ikan maskoki disajikan secara deskriptif dalam bentuk gambar untuk identifikasi Lernaea dan dalam bentuk tabel untuk derajat infestasi. Hasil penelitian yang dilakukan di Desa Karang Rejo, Desa Ploso Kandang dan Desa Wajak hanya ditemukan satu spesies saja, yaitu Lernaea cyprinacea. Derajat infestasi dari serangan Lernaea cyprinacea di tiga desa tersebut tergolong dalam infestasi ringan.
Analisis Prevalensi dan Intensitas Pseudorhabdosynochus coioidesis Pada Ikan Kerapu Bebek (Cromileptes altivelis) di Perairan Lampung dan Situbondo Hervina Benazir Ardiyanti; Sri Subekti; Kusnoto
Journal of Aquaculture Science Vol 7 No 1 (2022): Journal of Aquaculture Science
Publisher : Airlangga University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31093/joas.v7i1.205

Abstract

Humpback Groupers (Cromileptes altivelis) are a type of fish with great economic value that has the potential to be developed in Indonesia. Pseudorhabdosynochus coioidesis is one of the species that has been documented to infest Humpback Groupers. The purpose of this study was to determine the prevalence and severity of the parasitic worm Pseudorhabdosynochus coioidesis in Humpback Groupers in Lampung and Situbondo seas. 75 Humpback Groupers (20-35 cm) were sampled from Lampung waters, and 60 from Situbondo seas. The data were processed descriptively and presented in tabular form. The results showed that there was Pseudorhabdosynochus coioidesis worm infestation on the gills of Humpback Groupers in Lampung and Situbondo waters. The prevalence value of Pseudorhabdosynochus coioidesis in Humpback Groupers in Lampung waters was 93% with the almost always category and the intensity value was 1.48 in the low category while in Situbondo waters the prevalence value was 95% with the almost always category and the intensity value was 1.33 with low category. The low-intensity result indicates that the presence of Pseudorhabdosynochus coioidesis is not as dangerous to crop yields, but it should still be monitored.
Prevalence of Helminthiasis in Etawa Crossbreed Goat in Kalipuro Sub-District Banyuwangi Regency khoirul - Arifin; moh. arief ma'ruf; Kusnoto
Journal of Parasite Science (JoPS) Vol. 6 No. 1 (2022): Journal of Parasite Science
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (558.851 KB) | DOI: 10.20473/jops.v6i1.34303

Abstract

Etawa crossbreed goat are meat and milk-producing livestock that can adapt to the climate in Indonesia. The barriers to raising goats include the problem of disease which is a direct influence on livestock life. This study aims to determine the prevalence of helminthiasis in Etawa Crossbreed Goat in Kalipuro District, Banyuwangi Regency. The study was conducted in January until February 2019 using 100 fecal samples. The samples examination in was performed the laboratory of Instrument Airlangga University PSDKU in Banyuwangi Prodi of Veterinary Medicine, using native, sedimentation and floatation methods. Data obtained from this study were presented descriptively and analyzed by Chi-Square test. Based on examination results, prevalence of helminthiasis in Etawa Crossbreed Goat was 43 %, which is dominately by Moniezia spp. (17%), Haemonchus spp. (15%), Trichuris spp. (9%), Strongyloides spp. (7%), Trichostrongylus spp. (7%). Furthermore, it was suggested that anthelmintic and inspection of helminthiasis in those area given periodically and continuously.  
Study on the Morphology of Fasciola gigantica and Economic Losses due to Fasciolosis in Berau, East Kalimantan Muhammad Rofi' Prasetya; Setiawan Koesdarto; Nunuk Dyah Retno Lastuti; Lucia Tri Suwanti; Kusnoto Kusnoto; Muchammad Yunus
Biosaintifika: Journal of Biology & Biology Education Vol 11, No 1 (2019): April 2019
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v11i1.18201

Abstract

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.
Gambaran Darah dan Histopatologi Insang, Usus Dan Otak Ikan Koi (Cyprinus carpio Koi) yang Diinfeksi Spora Myxobolus koi secara Oral [Haematology and Histopatology of Gills, Intestine And Brain Koi Fish (Cyprinus carpio Koi) Myxobolus koi Orally Infected] Gunanti Mahasri; Kusnoto Kusnoto; Era Insivitawati
Jurnal Ilmiah Perikanan dan Kelautan Vol. 7 No. 2 (2015): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v7i2.11210

Abstract

Abstract Koi fish (Cyprinus carpio koi) is one of the ornamental fish are preferred. One of the obstalces in the koi fish is a disease caused by infection myxobollus. This research to determine the effect of the infection myxobollus by oral in blood picture and histopathology. Haematology and histopathology is an indicator of the health of the fish to diagnose disease and to determine changes in the fish tissue. The research was conducted in Politeknik Kelautan dan Perikanan, dan Balai Karantina Ikan Kelas I, Juanda Surabaya from October to December 2014. This study uses the 5 treatments and 5 replications with different doses of infection (0,60,80,100 and 120 spora/fish). Data were analyzed with Annova haematology, followed by Duncan 5%. While histopathology were analyzed by semiquantitative scoring mothod used to determine the degree of histology Kluschal Wallis. The result showed that the haematology has significant result, white histopathology signify the presence of significant damage.
Pengaruh Suhu Penyimpanan Terhadap Kerusakan Spora Myxobolus koi [The Effect of Storage Temperature in Myxobolus koi Spore Damage] Gunanti Mahasri; Titom Gusmana Putra Perdana; Kusnoto Kusnoto
Jurnal Ilmiah Perikanan dan Kelautan Vol. 11 No. 1 (2019): JURNAL ILMIAH PERIKANAN DAN KELAUTAN
Publisher : Faculty of Fisheries and Marine Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jipk.v11i1.12270

Abstract

AbstrakSpora Myxobolus koi dapat mengalami kerusakan apabila disimpan dalam kondisi penyimpanan yang kurang baik. Penelitian ini bertujuan untuk mengetahui pengaruh suhu penyimpanan terhadap kerusakan spora Myxobolus koi serta untuk mengetahui suhu optimum untuk penyimpanannya. Penelitian ini dilakukan dengan metode rancangan acak lengkap (RAL) sebagai rancangan percobaan. Perlakuan yang digunakan adalah penyimpanan spora Myxobolus koi pada suhu kamar (28-34) oC, Refrigerator (2-4) oC, dan Freezer (-5 hingga -10) oC, dengan ulangan sebanyak 6 kali. Penyimpanan ini dilakukan selama 30 hari. Parameter utama yang diamati adalah prosentase spora Myxobolus koi yang rusak. Parameter penunjang yang diamati adalah tipe kerusakan spora Myxobolus koi. Analisis data menggunakan ANAVA (Analisis Varian) dan dilanjutkan dengan Uji Jarak Berganda Duncan untuk mengetahui suhu optimum untuk penyimpanan spora Myxobolus koi. Berdasarkan hasil penelitian diketahui bahwa peyimpanan pada suhu yang berbeda berpengaruh terhadap prosentase kerusakan spora Myxobolus koi. Kerusakan spora Myxobolus koi tertinggi terjadi pada suhu kamar (28-34) oC mencapai angka 68,91% dan nilai kerusakan terrendah terjadi pada refrigerator (2-4) oC yaitu 29,91%. Spora Myxobolus koi dapat disimpan pada refrigerator dan lemari pembeku. AbstractMyxobolus koi spores can be damaged if stored in poor conditions. This study aimed to determine the effect of storage temperature on Myxobolus koi spores and to determine the optimum temperature for storage. This research was conducted using a completely randomized design method (CRD) as an experimental design. The treatments used were storaged Myxobolus koi spores at room temperature (28-34oC), Refrigerator (2-4oC), and Freezers (-5 to -10oC), with replications 6 times. This storage was carried out for 30 days. The main parameter observed was the percentage of damaged Myxobolus koi spores. The supporting parameters observed were the type of Myxobolus koi spore damage. Data analysis using ANAVA (Analysis of Variance) and continued with Duncan's Multiple Distance Test to find out the optimum temperature for storage of Myxobolus koi spores. Based on the results of the study, it was found that storage at different temperatures affected the percentage of damage to Myxobolus koi spores. The highest damage of Myxobolus koi spores occurred at room temperature (28-34oC) reaching 68.91% and the lowest damage value occurred at refrigerator (2-4oC) which was 29.91%. Myxobolus koi spores can be stored in a refrigerator and freezer
Co-Authors Abdul Samik Achmad Hasan Sahani Agnes Theresia Soelih Estoepangestie Akbar Wijaya Putra Purnama Andhika Yudhantama Subroto Andriani Dwi Siswarini Arimbi Arimbi Arum Puspitasari Bambang Sektiari Lukiswanto Bernideta Dewi Kriswijayanti Boedi Setiawan Bryan Ahmad Affan Lubis Budiarto Budiarto Candra Dwi Atma Chusniati, Sri Cintia Larasati Dhio Asmaydo Didik Handijatno Diyah Ayu Candra E Djoko Poetranto E Djoko Poetranto Eduardus Bimo Aksono Herupradoto Eka Pramyrtha Hestinah Ellza Agatha Damayanti Endang Suprihati Eny Coolfina Simarmata Era Insivitawati Erma Safitri Fania Selfiannisa Firman Hadi Fanani Gunanti Mahasri Hana Eliyani Hani Plumeriastuti Hari Suprapto Hartono Hartono Hemasayu Nirmala Putri Herry Agoes Hermadi Hervina Benazir Ardiyanti Ine Karni Iwan Sahrial Hamid Jihaan Haajidah Jola Rahmahani khoirul - Arifin Kismiyati , Koesnoto Supriandono Kuncoro Puguh Santoso Lianny Nangoi Lucia Tri Suwanti, Lucia Tri Mas’ud Hariadi Miftahul Jannah Mochamad Lazuardi moh. arief ma'ruf Mohammad Sukmanadi Muchammad Yunus Mufasirin Muhammad Rofi' Prasetya Muhammad Rofi' Prasetya Ngakan Made Rai Widjaja Nisa’ Rachmaningtyas Putri Nove Hidayati Novia Intan Kurnia Nunuk Dyah Retno Lastuti Nunuk Dyah Retno Lastuti Ogen Sea Poedji Hastutiek Primarizky, Hardany Rahmi Sugihartuti Ratna Damayanti Retno Bijanti Retno Bijanti Ria Nikmatul Jannah Sardjana, I Komang Wiarsa Sesa Puput Febriyanti Setiawan Koesdarto Setiawati Sigit Soeharsono Soeharsono Sri Mulyati Sri Mumpuni Sosiawati Sri Subekti Suherni Susilowati Sunarso, Agus Talita Yuanda Reksa Titom Gusmana Putra Perdana Tri Bhawono Dadi Tutik Juniastuti Tyasningsih, Wiwiek Vanna Lidya Kharisma Vindo Rossy Pertiwi Warda Nafalizza Efendi