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All Journal Tropical Medicine Journal Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Pharmaciana: Jurnal Kefarmasian Jurnal Teknosains Program Kreativitas Mahasiswa - Penelitian Indonesian Journal of Biotechnology Majalah Obat Tradisional Science and Technology Indonesia Indonesian Journal of Chemistry Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Molluca Journal of Chemistry Education (MJoCE) Jurnal Penelitian Pendidikan, Psikologi Dan Kesehatan (J-P3K) Journal of Multidisciplinary Applied Natural Science Occupational and Environmental Medicine Journal of Indonesia Engagement: Jurnal Pengabdian Kepada Masyarakat Indonesian Journal of Pharmacology and Therapy

Eti Nurwening Sholikhah

Departement Of Pharmacology And Therapy, Faculty Of Medicine, Public Health, And Nursing, Universitas Gadjah Mada

Author-ID : 3661076

Religion Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Education Energy Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences

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Cytotoxic activity of simvastatin in T47D breast cancer cell lines and its effect on cyclin D1 expression and apoptosis Bayu Putra; Mae Sri Hartati Wahyuningsih; Eti Nurwening Sholikhah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 49, No 2 (2017)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Background: Statins (HMG-CoA Inhibitors) is a drug used for decreasing plasma cholesterol levels and used in therapy to prevent coronary artery disease. Research in animals and epidemiological studies showed that statin therapy can decrease risk against cancer associated with cholesterol. Based on that result then research of cytotoxic activity against simvastatin knowing cultur T47D breast cancer cells and his influence in decreasing expression of cyclin D1 and induction of apoptosis has been done.Research objectives: The aims of this research is to prove activities of simvastatin against T47D breast cancer cell culture, especially to examine cytotoxic activity, cyclin D1 expression, and simvastatin effect in apoptotic induction.Research method: The type of this research is quasi experiment with using posttest with non-equivalent control group design. Cytotoxicity test performed on T47D breast cancer cell cultures using MTT assay to determine IC50 values after given simvastatine. Expression of cyclin D1 and apoptosis induction test detected using flow cytometry with antibody monoclonal anti-cyclin D1 and Annexin V-Pi, then analyzed by FACS-Calibur program.Results: Simvastatin has cytotoxic effect against T47D breast cancer cells with IC50 values 25.25 µg/mL. Simvastatin with concentrations of 6.31; 12.62; 25.25 and 50.5 µg/mL was able to decrease the cyclin D1 expression. Furthermore, simvastatin can induce apoptosis with EC50 values 26.96 µg/mL in T47D breast cancer cells.Conclusion: Simvastatin has cytotoxic activity of in T47D breast cancer cells and decreasing cyclin D1 expression and inducing apoptosis activity in T47D breast cancer cells.Keywords: simvastatin, cytotoxic, cyclin D1, apoptotic, T47D.

Indonesian medicinal plants as sources of secondary metabolites for pharmaceutical industry Eti Nurwening Sholikhah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 48, No 4 (2016)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Medicinal plants are widely used in traditional medicine in both underdeveloped anddeveloping countries in the word until now. Some secondary metabolites isolated frommedicinal plants have been developed as modern drugs. New antimalarial artemisinin isan example of modern medicine that developed from Artemisia annua L, a plant usedin China since 4,000 years ago. Indonesia is endowed with a rich natural resource.The biodiversity comprises thousands plant species. Therefore, Indonesian flora andfauna are a remarkable opportunity for the development of secondary metabolites forpharmaceutical industry. The Indonesian National Agency for Drug and Food Control,Republic of Indonesia (Badan Pengawas Obat dan Makanan, Republik Indonesia = BPOMRI) classifies traditional medicine into three classes, namely jamu (Indonesian indigenoustraditional medicine), standardized herbal medicine and phytopharmaca based on itsscientific evidences. The BPOM also determined 9 medicinal plants for focusing researchfor drug development. This paper discusses some secondary metabolites and theirpharmacological activities of the following 9 selected Indonesian medicinal plants namelyPiper retrofractum Vahl, Andrographis paniculata Ness, Curcuma xanthorrhiza, Psidiumguajava L, Syzigium polyanthi, Morinda citrifolia, Guazuma ulmifolia Lamk, Zingiberofficinale, and Curcuma domestica collected from various publications. Furthermore,several modern drugs derived from medicinal plants are also discussed.

Effects of ciprofloxacin concentrations on the resistance of uropathogen Escherichia coli: in vitro kinetics and dynamics simulation mode Maya Dian Rakhmawatie; . Mustofa; Eti Nurwening Sholikhah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 52, No 3 (2020)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

Ciprofloxacin is recommended for complicated urinary tract infection (UTIs) caused by multidrug-resistant pathogens included Escherichia coli. However, its optimum dose for UTIs remains uncertain that may cause the bacterial resistance. This study was conducted to evaluate the effects of ciprofloxacin concentrations on the resistance of E. coli. The in vitro pharmacokinetic/pharmacodynamic (PK/PD) models of ciprofloxacin 750 mg oral dose twice a day for one daywas compared to that dose of 500 mg twice a day for three days.Pharmacokinetic parameters i.e.AUC0-24 and Cmax. and pharmacodynamic parameter i.e. MIC of ciprofloxacin against E. coli which previously had MIC of 0.5 µg/mL were determined. The PK/PD parameters combination of ciprofloxacin included AUC0-24/MIC, Cmax/MIC, and T>MIC ratio were used to evaluate its antimicrobial activities which was measured based on kill and re-growth rates of bacterial colony after the ciprofloxacin administration. The result showed that MIC value against E. coli increase to 8-16 and 32-64 µg/mL after ciprofloxacin 750 and 500 mg administration, respectively, indicating the emergence of resistance. Both doses of ciprofloxacin were able to reduce the number of bacterial colony in the first two hours administration. However, after two hours administration, those both doses could make re-growth of bacterial colony. The value of AUC0-24/MIC (120.42±1.27 vs.92.62±9.36), Cmax/MIC (4.75±0.21 vs. 3.26±0.30), and (T>MIC 89.58±7.22 vs. 76.39±9.39) after ciprofloxacin administration at dose of 750 mg were higher than those at dose of 500 mg. The increase of AUC0-24/MIC and Cmax/MIC values could reduce the number of bacteria colony, however could not for T>MIC value. In conclusion, the AUC0-24/MIC and Cmax/MIC parameters of ciprofloxacincan be used to evaluate its activity. In addition, ciprofloxacin twice per day at dose 500 mg for three days and 750 mg for one day are not different in the inhibition of E. coli resistance emergence.

Potential secondary metabolite analysis of soil Streptomyces sp. GMR22 and antibacterial assay on Porphyromonas gingivalis ATCC 33277 Hera Nirwati; Ema Damayanti; Eti Nurwening Sholikhah; . Mustofa; Jaka Widada
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 54, No 2 (2022)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.19106/JMedSci005402202202

Infectious diseases caused by oral pathogenic bacteria are currently a serious problem due to the increasing incidence of antimicrobial resistance. Streptomyces sp. GMR22, a soil actinobacterium which has large-genome size. In previous studies, it was known to have antifungal, and antibiofilm activity on Candida albicans. However, its antibacterial activity on oral pathogenic bacterium, Porphyromonas gingivalis is not clear. This study aimed to identify potential active compound based on genome mining analysis and to evaluate the antibacterial activity of GMR22 extract on P. gingivalis ATCC 33277. Potential active compounds and biosynthesis gene clusters were analysis using antiSMASH version 5. Antibacterial activity assay was carried out by the microdilution method on P. gingivalis ATCC 33277. Based on genome mining analysis polyketide synthase (PKS), the Streptomyces sp. GMR22 is the abundant BGCs (35%) and has large-predicted compounds which have antibiotic-antibacterial activity (22.9%). On antibacterial assay, chloroform extract of GMR22 at 7.8 – 62.5 µg/mL has high antibacterial activity on P. gingivalis compared to other extracts. Soil Streptomyces sp. GMR22 bacterium has biotechnological potential to produce active compounds for antibacterial.

UJI IRITASI MATERIAL COBALT CHROMIUM SEBAGAI MATERIAL DASAR BONE PLATE UNTUK REKONSTRUKSI MANDIBULA Dian Permata Sari; Eti Nurwening Sholihah; Muhammad Kusumawan Herliansyah
Jurnal Teknosains Vol 7, No 2 (2018): June
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/teknosains.8162

The mandibular bone defect can cause speech disorder, mastication malfunction, and aesthetic problems. The application of plate for mandibular reconstruction can improve the function disorder. Plate material must be strong, resilient, able to adapt to the bone surface, and has good biocompatibility. Cobalt chromium is a cobalt base alloys are often used for implant materials. It has good mechanical properties, biocompatibility, and corrosion resistance. The aim of this study was to investigate the effect of local irritation and tissue response on material cobalt chromium. This study used three albino rabbits were injected by the material powder in polar and nonpolar solvents with intracutaneous injection method, then the treatments were observed on the first, second, and third days. Erythema and edema that occurs were scored based on grading system for intracutaneous (intradermal) reactions. Friedmann test showed no significant difference between treatment groups (p> 0.05). The results showed that erythema and edema arising from irritant reactions are still within normal limits. Irritation occurs due to the release of cobalt chromium ions which trigger hypersensitivity reactions and result in systemic immunity.

Effects of Poly-herbal Tablet as Herbal Medicine on Lipid Level Woro Rukmi Pratiwi; Eti Nurwening Sholikhah; Dwi Aris Agung Nugrahaningsih; Mia Munawaroh Yuniyanti; Mustofa Mustofa; Ngatidjan Ngatidjan
Majalah Obat Tradisional Vol 24, No 1 (2019)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Dyslipidemia is one of the high-risk factors that cause cardiovascular disease and the prevalence is increasing all the time. Garlic (Alii sativi) has been suggested to reduce serum lipid level. Poly-herbal is herbal medicine that has been widely used in Indonesia, and it contains Alii sativi, Belericae fructus, Curcumae aeroginosae, and Amomi fructus extract. This study aimed to evaluate the effects of poly-herbal tablet on lipid levels in dylipidemia subjects. The study was conducted for 6 weeks on 58 dyslipidemic subjects. The subject was divided into 2 groups. Group 1 was given Poly-herbal with the dose 2x1200 mg, and group 2 was not given anti-dyslipidemic drugs (control group). Serum lipid level (total cholesterol, LDL, HDL, and triglycerides) were checked before the treatment (H0) and after the treatment (M6). There was a significant difference on the reduction of serum lipids level between the group that given Poly-herbal vs control group, respectively total cholesterol (-12.04±3.2 vs 8.38±4.3; p=.000), LDL (-4.42±2.8 vs 6.93±4.7; p=.049) and HDL (-2.62+1.0 vs 3.31+1.4; p=.005). Triglyceride level on Poly-herbal group is not significantly decreased. In Poly-herbal group there was also a decrease in HDL levels which was not statistically significant (-2.62±1, p=.015). This is probably due to the HDL baseline in the control group included in the normal HDL category (57.38 ± 14.9) so that the HDL levels after being given treatment did not increase. This study suggests that using Poly-herbal in dyslipidemia subjects can reduce the level of total cholesterol and LDL.

Effects of Polyherbal Tablet for Hypertensive Patients Woro Rukmi Pratiwi; Eti Nurwening Sholikhah; Dwi Aris Agung Nugrahaningsih; Mia Munawaroh Yuniyanti; Mustofa Mustofa; Setyo Purwono
Majalah Obat Tradisional Vol 25, No 3 (2020)
Publisher : Faculty of Pharmacy, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/mot.62363

Hypertension is a degenerative disease whose prevalence is high and continues to increase over time. Polyherbal tablet have long been used by Indonesian people for hypertension drugs containing garlic (Alium sativum), jelawe (Belericae fructus), temu ireng rhizomes (Curcumae aeruginosae) and kapulaga (Amomi fructus) extracts. This study aims to determine the effect of polyherbal tablet on hypertensive patients. The subjects of 29 hypertensive patients participated in a open-randomized-without comparison-study for 6 weeks. Subjects were divided into 3 groups, each subject received polyherbal tablet with a dose of 2x600 mg (group 1), 2x1200 mg (group 2) and 2x2400 mg (group 3). Blood pressure was monitored on D-0, D-3, D-5, D-7, W-2, W-3, W-4, W-5 and W-6. There was a significant decrease in systolic and diastolic blood pressure at the sixth week in all three treatment groups. The highest decrease in systolic blood pressure was in group 1 while diastolic blood pressure was in group 2 (-22.94 ± 5.871 and 13.13 ± 4.324 respectively). The lowest reduction in systolic and diastolic blood pressure occurred in the third group (-15.07 ± 3,519 and 8.98 ± 3,394 respectively). This study suggests that polyherbal tablet contains garlic (Alii sativi), jelawe (Belericae Fructus), temu ireng rhizome (Curcumae aeruginosae) and cardamom (Amomi Fructus) can reduce blood pressure in patients with hypertension.

SINTESIS DAN UJI AKTIVITAS SENYAWA (1)-N-(n-BUTIL)- DAN (1)-N-(t-BUTIL)-1,10-FENANTROLINIUM SEBAGAI SENYAWA POTENSIAL ANTIMALARIA BARU Ruslin Hadanu; Sabirin Mastjeh; Mustafa Mustafa; Eti Nurwening Sholikhah; Mahardika Agus Wijayanti
Molluca Journal of Chemistry Education (MJoCE) Vol 1 No 1 (2011): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol1iss1pp45-53

The synthesis of (1)-N-(n- buthyl)- dan (1)-N-(t-buthyl)-1,10-phenanthrolinium with 1,10-phenanthroline monohydrate as starting material through two steps has been carried out. The first step of reaction is chlorination and bromination of n-buthyl-alcohol/t-buthyl alcohol using HCl and HBr, respectively. The result of reaction is nbuthyl bromide 2 (colourless liquid, 70.92%) and t-buthyl chloride 4 (colourless liquid, 92.36%), respectively. The second step of reaction is alkylation of 1,10-phenanthroline 5 using n-buthyl bromide and t-buthyl chloride reagents that its was refluxed for 21 and 23 h, respectively. The results of reaction are (1)-N-(n- buthyl)-1,10fenantrolinium bromida 6 and (1)-n-(t-buthyl)-1,10-fenantrolinium chloride 7 in yield from 84.70% and 78.16%, respectively. The results of testing in in vitro antiplasmodial activity at chloroquine-resistant P. falciparum FCR3 strain to (1)-N-(n-buthyl)- and (1)-N-(t-buthyl)-1,10- phenanthrolinium obtained that (1)-N-(n-buthyl)-1,10phenanthrolinium bromide 6 has higher antimalarial activity (IC50 : 0.03Â±0.01 ÂµM) than antimalarial activity of (1)-n-(t-buthyl)-1,10-phenanthrolinium chloride 7 (IC50 : 2.09Â±0.08 ÂµM). While, the results of testing in in vitro antiplasmodial activity at chloroquine-resistant P. falciparum D10 strain to (1)-N-(n-buthyl)- and (1)-N-(t-buthyl)1,10-f phenanthrolinium obtained that (1)-N-(n-butil)-1,10- phenanthrolinium bromide 6 has higher antimalarial activity (IC50 : 1.40Â±0.82 ÂµM) than antimalarial activity of (1)-n-(t-buthyl)-1,10- phenanthrolinium chloride 7 (IC50 : 2.24Â±0.05 ÂµM

SINTESIS DAN UJI AKTIVITAS ANTIMALARIA SENYAWA (1)-N-BENZIL-1,10- FENANTROLINIUM BROMIDA Ruslin Hadanu; Sabirin Mastjeh; Jumina Jumina; Mustafa Mustafa; Eti Nurwening Sholikhah; Mahardika Agus Wijayanti
Molluca Journal of Chemistry Education (MJoCE) Vol 1 No 2 (2011): Molluca Journal of Chemistry Education (MJoCE)
Publisher : Program Studi Pendidikan Kimia FKIP Universitas Pattimura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30598/MJoCEvol1iss2pp92-102

A synthetic methods was employed to prepare (1)-N-benzyl)-1,10-phenanthroliniumbromide 4 from 1,10phenanthroline 3 and benzylchloride 1 through substitution and alkylation reactions. The compound (1)-Nbenzyl)-1,10-phenanthroliniumbromide 4 was tested through antiplasmodial test. The benzylbromide 2 was synthesized through substitution reaction of benzylchloride 1 and NaBr which has a yield of 74.25%. The (1)-N-benzyl)-1,10-phenanthroliniumbromide 4 was synthesized from 1,10-phenanthroline 3 using benzylbromide 2 reagents which refluxed for 8 hours with 84.04% yields. The results of testing in in vitro antiplasmodial activity at chloroquine-resistant Plasmodium falciparum FCR3 strain to (1)-N-benzyl)-1,10- phenanthroliniumbromide 4 has high antimalarial activity (IC50 : 0.10Â±0.04 ÂµM)

EXPERIENTIAL LEARNING METHODS IMPROVES RATIONAL PRESCRIBING SKILL IN MEDICAL STUDENTS Mega Pandu Arfiyanti; Gandes Retno Rahayu; Eti Nurwening Sholikhah
Jurnal Pendidikan Kedokteran Indonesia: The Indonesian Journal of Medical Education Vol 10, No 2 (2021): July
Publisher : Asosiasi Institusi Pendidikan Kedokteran Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpki.56906

Background: Rational prescribing is an important skill for medical doctors. Many graduates still feel insufficiently prepared to make rational prescribing and fail to demonstrate the related clinical reasoning after graduation. For these reasons, it is important to improve the medical student teaching of rational prescribing. The aim of this study is proving the effectiveness of experiential learning methods to improve rational prescribing skills of medical students.Methods: This study used a one-group pretest-posttest design. Rational prescribing courses use experiential learning methods for 6 year medical students. Every student provided evaluation of rational prescribing and clinical reasoning through pretest and posttest, and we analyzed the results by paired t test.Results: The change in the rational prescribing skill of the students is significant between pretest and posttest. However, in the hypertension case the scores are not significant between pretest and posttest. Also, the scores of clinical reasoning based on drug interaction and evidence based methods are not significant in the hypertension and otitis media cases.Conclusions: Experiential learning methods can enhance rational prescribing skill in medical students but the students were still not able to choose appropriate medications based on drug interaction and evidence based approach.

Co-Authors Achmad Djunaidi Achmad Djunaidi Adisty Ridha Damasuri Adisty Ridha Damasuri ADITYA NUGRAHA Aditya Nugraha Aflifia Birruni Sabila Agus Dwi Ananto, Agus Anak Agung Ayu Niti Wedayani Anak Agung Ngurah Nata Baskara Andi Hairil Alimuddin Arta Farmawati Asysyifa, Azzahra Bayu Putra Chairil Anwar Chairil Anwar Danang Setia Budi Demianus Tafor Demianus Tafor Dian Permata Sari Dicky Moch Rizal Dicky Moch Rizal Dwi Aris Agung Nugrahaningsih Dwi Cahyani Ratna Sari E. Elsa Herdiana Murhandarwati Ema Damayanti Erna Kristin Erwin Astha Triyono Faris Hermawan Fatimi, Hana Anisa Fitria, Anggit Gandes Retno Rahayu Hanggoro Tri Rinonce Harizal Harizal Harno Dwi Pranowo Harno Dwi Pranowo Harno Dwi Pranowo Hera Nirwati Iin Narwanti Ikhsan Nur Salim Ikhsan Nur Salim Iqmal Tahir JAKA WIDADA Jaya, Sudi Indra Jeffry Julianus Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Jumina Kasta Gurning Kurniawan, Yehezkiel Steven Laela Hayu Nurani Mae Sri Hartati Wahyuningsih Mahardika Agus Widjayanti Mahardika Agus Wijayanti Mahardika Agus Wijayanti Mahardika Agus Wijayanti Mahardika Agus Wijayanti Marlinda, Wade Masriani . Maulina Diah Maulina Diah Maya Dian Rakhmawatie Mega Pandu Arfiyanti Mia Munawaroh Yuniyanti Muhammad Idham Darussalam Mardjan Muhammad Kusumawan Herliansyah Muhammad Yusron Maulana El-Yunusi Mujur Mujur Mustafa Mustafa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Mustofa Muthia Rahayu Iresha Muthia Rahayu Iresha Nendrowati Nendrowati Ngatidjan Ngatidjan Nurrahma, Bira Arumndari Nursofia, Baiq Ike Pramana Pananja Putra Pratiwi, Woro Rukmi Pratiwi, Woro Rukmi Priastomo, Yoga Primahana, Gian Priska Ernestina Tenda Priyangga, Krisfian Tata Aneka Pulung, Maria Ludya Ratna Sari RATNA SARI Respati Tri Swasono Rina Susilowati Rul Afiyah Syarif Ruslin Hadanu Sabirin Mastjeh Sabirin Mastjeh Sabirin Mastjeh Sabirin Mastjeh Sabirin Matsjeh Sabirin Matsjeh Samekto Wibowo Samekto Wibowo Setyo Purwono Sitarina Widyarini Susi Iravati Susi Iravati Syarifa, Cut Alima Teguh Triyono Titik Nuryastuti Tri Joko Raharjo Tri Murini Tri Ratnaningsih Wahyu Nitari Wahyu Widyaningsih Wibowo, Susalit Setya Widodo, Irianiwati Widya Wasityastuti Winarto Haryadi Wiyono, Tri Yacobus Christian Prasetyo Yogaswara, Radite Yudha, Ervan Yuniyanti, Mia Munawaroh Zulfa Faiqoh

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