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All Journal Jurnal Gizi dan Pangan Agrokreatif Jurnal Ilmiah Pengabdian kepada Masyarakat Jurnal Surya Medika JURNAL AGRIBISAINS Jurnal Perseda : Jurnal Pendidikan Guru Sekolah Dasar Indonesian Journal of Global Health research Journal of Agri Socio Economics and Business Electoral Governance : Jurnal Tata Kelola Pemilu Indonesia Jurnal Ilmiah Management Agribisnis (Jimanggis) Journal of Health and Nutrition Research Jurnal Pengabdian Masyarakat Bhinneka Al-DYAS : Jurnal Inovasi dan Pengabdian kepada Masyarakat Mestaka: Jurnal Pengabdian Kepada Masyarakat Jurnal AGRISEP: Kajian Masalah Sosial Ekonomi Pertanian dan Agribisnis Sriwijaya Journal of Agribusiness and Biometrics in Agriculture Research Plexus Medical Journal Agrifo: Jurnal Agribisnis Universitas Malikussaleh JIS: Journal ISLAMIC STUDIES Kesmas: Jurnal Kesehatan Masyarakat Nasional (National Public Health Journal) Jurnal Riset Manajemen
Yulia Sari
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Religion Agriculture, Biological Sciences & Forestry Humanities Biochemistry, Genetics & Molecular Biology Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Energy Environmental Science Health Professions Immunology & microbiology Languange, Linguistic, Communication & Media Mechanical Engineering Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Phytochemical Characterization and In Vitro Antioxidant Activity of Murraya Koenigii Leaf Extract Nafi'ah, Radifatun; Febrinasari, Ratih Puspita; Sari, Yulia
Journal of Health and Nutrition Research Vol. 4 No. 3 (2025)
Publisher : Media Publikasi Cendekia Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.56303/jhnresearch.v4i3.459

Abstract

Murraya koenigii (curry leaf) is a tropical plant known for its diverse phytochemical content and potent antioxidant activity. However, limited studies have provided comprehensive quantitative data on its bioactive compounds, especially from Indonesian varieties. This study aimed to quantitatively analyze flavonoid, saponin, and tannin content in young and mature curry leaves and evaluate their in vitro antioxidant activity using the DPPH assay. An experimental design was conducted from January to March 2025. Ethanolic extracts of young and mature M. koenigii leaves from Aceh were prepared via maceration. Phytochemical quantification was performed using spectrophotometric methods, and antioxidant capacity was assessed using the DPPH radical scavenging assay with IC₅₀ determination. Young leaves showed higher levels of total flavonoids (0.19%), saponins (3.34%), and tannins (5.77%) compared to mature leaves (0.13%, 2.53%, and 4.58%, respectively). However, mature leaf extract exhibited stronger antioxidant activity with an IC₅₀ value of 46.77 μg/mL (very strong category), while young leaves showed 53.14 μg/mL (strong category). This suggests that antioxidant efficacy is influenced more by compound maturity and synergistic effects than total concentration. Mature M. koenigii leaves have superior antioxidant potential despite lower phytochemical concentrations, highlighting the importance of compound quality and interactions between compounds.
Co-Authors Adzra, Ghaitsa Safira Amalina, Dian Amelia, Fadila Avelia, Annisa Azqia Wardani Delvianti Dian Retno Intan Didik Gunawan Tamtomo Diman, Banta Eka Maulidiya, Sherly Erni Purbiyanti, Erni Eti Poncorini Pamungkasari Fitria, Yanti Fuji Lestari, Etika Gultom, Angga Wibowo Ibrahim, Dwi Agustian Faruk Ilham Pamungkas Susilo, Ade Irawan, Nurul Anira Isbayu , Muhammad Jerry Antonio Khairul Fahmi Purba Khairunnisa, Salma Firyal LATIFAH Lestari, Etika Fuji M, Agussalim M. Huanza Manurung, Gugun Maulidia Tri Yuliani Muhammad Syukur Mujayanah, Ani Rosa Putri Ayu Nafi'ah, Radifatun Natalia, Vinda Negara, Khesara Sastrin Prasita Putri, Nurillla Elysa Putri, Rani Revina Ratih Puspita Febrinasari Revina Putri , Rani Ritonga, Utan Sahiro Shanti Listyawati Shofia, Alima Sianipar, Theresia Aprilia Sri Haryati Tafarini, Meitry Firdha Trisna Wahyu Swasdiningrum Putri Tusri, Yudi Y. K., Karmitasari Yeni Erita Yuliani, Maulidia Tri Yunita Yunita