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Journal : Journal of Tropical Marine Science

Toksisitas Ekstrak Daun mangrove Rhizophora stylosa dari Pulau Maspari Sumatera Selatan Muhtadi, Muhtadi; Ulqodry, Tengku Zia; Rozirwan, Rozirwan; Sarno, Sarno; Aryawati, Riris
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5679

Abstract

The leaves of R. stylosa are a potential source of bioactive compounds for health and pharmacology. The composition of bioactive compounds in the leaves can be influenced by the ecological conditions and geographical location of this small island. This study aims to evaluate the toxicity and composition of bioactive compounds that are toxic in mangrove leaf extracts of R. stylosa from Maspari Island in coastal South Sumatra. A total of 1 kg of R. stylosa leaf samples were taken from Maspari Island then washed thoroughly to remove dirt, cut into small pieces, and dried in the sun using the indirect sunlight method covered with black cloth. The dried leaf samples were then pulverized into powder, and as much as 100 grams of fine powder was macerated with 96% ethanol solvent in a ratio of 1:10 (b/v). The maceration process was carried out for 1 x 24 hours, then the maceration solution was filtered and evaporated using a rotary evaporator at 60°C to become a concentrated extract. Toxicity tests were carried out using the Brine Shrimp Lethality Test (BSLT) method, while the identification of toxic bioactive compounds was carried out through phytochemical tests, total phenol tests, and GC-MS analysis. The test results showed that the LC50 value of R. stylosa leaf extract was 393 mg/mL (moderately toxic). The total phenol content was 442.82 (gGA/g), and the bioactive compounds identified included alkaloids, flavonoids, saponins, terpenoids, and acids such as Hexadecanoic acid, methyl ester, and trans-13-Octadecenoic acid, methyl ester.
Toksisitas Ekstrak Daun mangrove Rhizophora stylosa dari Pulau Maspari Sumatera Selatan Muhtadi, Muhtadi; Ulqodry, Tengku Zia; Rozirwan, Rozirwan; Sarno, Sarno; Aryawati, Riris
Journal of Tropical Marine Science Vol 8 No 1 (2025): Journal of Tropical Marine Science (on going)
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jour.trop.mar.sci.v8i1.5679

Abstract

The leaves of R. stylosa are a potential source of bioactive compounds for health and pharmacology. The composition of bioactive compounds in the leaves can be influenced by the ecological conditions and geographical location of this small island. This study aims to evaluate the toxicity and composition of bioactive compounds that are toxic in mangrove leaf extracts of R. stylosa from Maspari Island in coastal South Sumatra. A total of 1 kg of R. stylosa leaf samples were taken from Maspari Island then washed thoroughly to remove dirt, cut into small pieces, and dried in the sun using the indirect sunlight method covered with black cloth. The dried leaf samples were then pulverized into powder, and as much as 100 grams of fine powder was macerated with 96% ethanol solvent in a ratio of 1:10 (b/v). The maceration process was carried out for 1 x 24 hours, then the maceration solution was filtered and evaporated using a rotary evaporator at 60°C to become a concentrated extract. Toxicity tests were carried out using the Brine Shrimp Lethality Test (BSLT) method, while the identification of toxic bioactive compounds was carried out through phytochemical tests, total phenol tests, and GC-MS analysis. The test results showed that the LC50 value of R. stylosa leaf extract was 393 mg/mL (moderately toxic). The total phenol content was 442.82 (gGA/g), and the bioactive compounds identified included alkaloids, flavonoids, saponins, terpenoids, and acids such as Hexadecanoic acid, methyl ester, and trans-13-Octadecenoic acid, methyl ester.
Co-Authors Abdul Kadir Afrianti, Nur Afni Agus Purwoko Altri Mulyani Amrullah, M. Fikri Andi Murlina Tasse Apriani, Elsa Fitria Apriyanto, Apriyanto Army, Fadilla Surya Bahri, Indra Purnama Budoyo, Soko Catur Putra Satgada, Catur Putra Dermiyati, Dermiyati Doni Setiawan Dwi Hardestyariki, Dwi Dwi Puspa Indriani Dwi Putriana Nuramanah Kinding Dwi Suheryanto Elisa Nurnawati Enggar Patriono, Enggar Erwin Nofyan, Erwin Febri Setiawan Hanifa Marisa Harmida Harmida hasana, nurdiah Herry Purnomo Ilfairah, Jihan Irene Kartika Eka Wijayanti Jamalam Lumbanraja Kamila Alawiyah Kartini, Bunga Kusumastuti, Dyah Laila Hanum Linirin Widiastuti, Endang Lutfi Istikharoh Masliani, Masliani Masruroh, Yulfah Mazidah Mazidah, Mazidah Moh. Rasyid Ridho Muhtadi Muhtadi Muizzaddin, Muizzaddin Natsir Sandiah Nita Aminasih Novpriansyah, Hery Nugroho Ekowati, Christina Okarda, Beni Oktarina, Yetty Pancasari, Adelia Rizki Parwanti, Sri Pirnanda, Dafid Pragustiandi, Guntur Reza Azizul Nasa Al Hakim Riris Aryawati riswanto riswanto Rohmawati Bareta, Ainun Rosma Hasibuan, Rosma Rozirwan . Rujito Agus Suwignyo Rustono - Saputro, Wahyu Adhi setiowati setiowati Siti Masreah Bernas Solekan , Muhamad Sri Mulyani Sriati Sriati, Sriati Sugeng Triyono Suheyanto Suheryanto Sumardi . Supriatin Supriatin Supriatin Supriatin, Supriatin Surni, Surni Sutrisno, Anas Syahrul Ganda Sukmaya T. Zia Ulqodry Tengku Zia Ulqodry Tri Haryanto Utomo, Muhajir Widjajanti, Haryati Ahda Wirda, Sahira Yuanita Windusari Yuliany, Eka Haryati Yunita Sari Zazili Hanafiah