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Journal : SIMBIOSIS

STRUKTUR HATI MENCIT (Mus musculus L.) SETELAH PEMBERIAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn.) Haryo Seto Wicaksono; Inna Narayani; Iriani Setyawati
SIMBIOSIS Vol 3 No 1 (2015)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

This research aims to determine the effect red calliandra leaf extract on weight, anatomy andhistology of male mice liver. Treatment was administered orally with varying doses (2, 4, 6mg/ kg). Control grups was given 0,9% NaCl. This study used a completely randomizeddesign (CRD), consisting of 12 groups with three replications. Histological observationsshowed that red calliandra leaf extract significantly affected the damage of male mice liver.Damage level of nucleus pyknotic and fatty degeneration increased in line with the increasingdoses in treatment for 7 and 21 days, as well as the dilation of central venous in treatment for7 days. The damage increase in line with the leght of treatment in the fatty degeneration (dose2 mg/kg bw), nuclues pyknotic (doses 2 and 6 mg/kg bw), infiltration of inflammatory cells(dose 4 mg/kg bw), sinusoid congestion (doses 2, 4 and 6 mg/kg bw), and central venousdilatation (dose 2 and 4 mg/ kg bw).Keywords : Red calliandra, male mice, liver histology
HUBUNGAN ANTROPOMETRI TULANG PANJANG TERHADAP TINGGI BADAN MAHASISWA SUKU BATAK TOBA DI KOTA BEKASI Ezra Oktavia Ambarita; Iriani Setyawati; Dwi Ariani Yulihastuti
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (342.993 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i01.p03

Abstract

Forensic anthropology is the application of a specific branch of biological anthropology based on human bones and anatomy. Anthropometry is a method of measuring body parts. Height measurement is a forensic anthropological parameter that helps build a person's biological profile. Height can be determined by the size of the long bones including the humerus, radius, ulna, femur, tibia, and fibula. This research on height was carried out on Batak Toba students. This study aims to determine the anthropometric relationship of long bone length to the height of Batak Toba students in the city of Bekasi. This research is an analytic observational study with a cross sectional approach. This research was conducted in the city of Bekasi with the research subjects totaling 60 students consisting of 30 men and 30 women with an age range of 18-22 years from the Toba Batak tribe who did not have bone disorders. Samples were taken by purposive sampling technique. Data analysis was performed by Pearson Correlation (r) test. The parameters analyzed in this study were length of humerus, radius, ulna, femur, tibia, fibula, height, sitting height, weight, and body mass index. The results of the Pearson Correlation test analysis (r) showed that there is a significant relationship between the length of the humerus, radius, ulna, femur, tibia, fibula and sitting height with a strong to perfect correlation, while the relationship between body mass index and height there is no significant difference and there is no correlation to Batak Toba students in the city of Bekasi. Keywords: height, long bones, anthropometry, Batak Toba
TINGGI DAN BERAT BADAN SISWA UMUR 10-12 TAHUN DI SEKOLAH PEDESAAN DAN KOTA : SD DI DESA PINANG SEBATANG TIMUR DAN KOTA PEKANBARU PROVINSI RIAU Fita Efi Rafelina Siallagan; I Ketut Junitha; Iriani Setyawati
SIMBIOSIS Vol 10 No 1 (2022)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.39 KB) | DOI: 10.24843/JSIMBIOSIS.2022.v10.i01.p08

Abstract

ABSTRACT The difference in height and weight is influenced by genetic traits and environmental factors. The study population was primary school children from one elementary school in the area of Pinang Sebatang Timur Village, Tualang Regency and one elementary school in urban areas covering Pekanbaru City. The two research areas are located in the province of Riau. The research sample was 60 students from Nusantara elementary school in Pinang Sebatang Timur Village and 60 students from Santa Maria elementary school, which consisted of (30 boys and 30 girls). The age range of the probandous is about 10-12 years old. The method that is being used for the research is purposive sampling method using online survey. The parameters studied were height, weight, age, parental education, parental occupation, parental income, body mass index (BMI), age of manarche and first wet dreams. Body Mass Index (BMI) was calculated by dividing body weight (kg) by height square (m2).According to the Center for Disease Control and Prevention, BMI is categorized based on 4 categories, namely underweight (<18), normal (18-25), overweight (25-27), and obese (>27). The average of heights, weights and body mass indexes of male and female students in Pekanbaru city are higher than in the Pinang Sebatang Timur’s village. Factors that are affecting student’s heights and weights are their parent’s job, their parent’s allowances, heights, the age of menarche on the female children or wet dream on the male children, and also the physical activities the students do. Keywords: Growing pattern, height, weight, age, Body Mass Index (BMI)
PENGARUH PEMBERIAN EKSTRAK TAUGE DITAMBAH MADU TERHADAP KUALITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) I Wayan Wena Diartha; Ni Wayan Sudatri; Iriani Setyawati
SIMBIOSIS Vol 4, No 1 (2016)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

Bean sprouts, vegetables that are often consumed, contain vitamin E, C, riboflavin, niacin, folate, and some minerals suchas selenium, manganese, zinc and iron. Honey contains monosaccharide i.e. fructose, sucrose, glucose, and maltose. Thisstudy aimed to investigate the effect of bean sprout extract added with honey to the spermatozoa quality of male mice and themost concentration that affected. This research used a completely randomized design (CRD). Twenty male mice divided intofour groups: control (aquadest), P1 (25 mg/g BW bean sprout extract + 0.07 ml honey), P2 (50 mg/g BW bean sprout extract+ 0.07 ml honey) and P3 (75 mg/g BW bean sprout extract + 0.07 ml honey). The extracts were given 0.3 ml/mice/day for 30days. The variables that are observed were spermatozoa motility, morphology, viability and also amount of spermatozoa. Datawere analyzed with one way ANOVA and followed by Duncan Multiple Range Test (DMRT). The results showed that beansprout extracts added with honey gave positive effects by increasing type A motility of spermatozoa but decreased type Dmotility. The treatments also increased the amount of spermatozoa significantly. However, 75 mg/g BW bean sprout extract +0.07 ml honey did not affect spermatozoa morphology and viability of male mice (Mus musculus L.). The optimal dose thataffected sperm quality in this research was 50 mg/g BW bean sprout extract + 0.07 ml honey.Keywords : honey, Mus musculus L, bean sprout extracts, the quality of spermatozoa
PENGARUH DOSIS DAN LAMA PERLAKUAN EKSTRAK DAUN KALIANDRA MERAH (Calliandra calothyrsus Meissn.) TERHADAP STRUKTUR HISTOLOGI GINJAL MENCIT (Mus musculus L.) Nur Assiam; Iriani Setyawati; Sang Ketut Sudirga
SIMBIOSIS Vol 2 No 2 (2014)
Publisher : Jurusan Biologi Universitas Udayana

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Abstract

This research aims to determine the effect of red calliandra (Calliandra calothyrsusMeissn.) leaf extrac on the histological structure of the kidney of mice (Mus musculus L.).Treatment was administered orally with varying doses. This research used a CompletelyRandomized Design in factorial pattern of two factors, doses (0 or control, 2, 4, and 6 mg/kgBW) and length of treatment (7, 14, and 21 days), so there are 12 combination groups with 3replications of each. Organ was collected on days 8, 15, and 22 to observe histologicalstructure of the kidney. Renal histological observation of edema, Bowman’s spaceconstriction, and protein deposition, showed no correlation between both factors, but a veryreal correlation occurs in the damage of fatty degeneration, hemorrhage, and nucleuspyknotic. Histological observation of glomerular congestion and infiltration of inflammatorycells did not show any correlation between dose and duration of treatment.Keywords: red calliandra, histopathology of kidney, male mice
Biokimia Darah Tikus (Rattus norvegicus) Betina Ovariektomi setelah Perlakuan Ekstrak Daun Kaliandra Merah (Calliandra calothyrsus Meissn.) Iriani Setyawati; Ngurah Intan Wiratmini; Ni Putu Ayu Dewi Wijayanti
SIMBIOSIS Vol 7 No 1 (2019)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (399.583 KB) | DOI: 10.24843/JSIMBIOSIS.2019.v07.i01.p03

Abstract

Red calliandra (Calliandra calothyrsus Meissn.) leaf have the potential as phytoestrogens. In this study, adult female ovariectomized rats (as a model of menopause women) were given calliandra leaf extract containing estrogenic flavonoids. This study was an experimental design with a Completely Randomized Design (CRD). Forty-five ovariectomized female rats were divided into five groups i.e. untreated control (K), and the treatment group given 80% of C. calothyrsus ethanolic leaves extract doses 25 mg/ kg bw (P1), 50 mg/ kg bw (P2), 75 mg/ kg bw (P3) and 100 mg/ kg bw (P4). The test suspension was given once a day at 24-hour intervals by gavage as much as 0.5 ml/rat/day for two months. At the end of the treatments, blood was collected for blood biochemical tests including hemoglobin levels, hematocrit values, blood glucose and proteins levels. The results showed that C. calothyrsus leaf extract did not negatively affect the health status of the animals through blood biochemical parameters, i.e. hemoglobin levels, hematocrit values, and glucose and protein levels of ovariectomized female rats.
COFFEE DECREASED THE ACTIVITY OF ALT AND AST BLOOD SERUM OF ALCOHOLIC WISTAR RATS I Made Sara Wijana; I Gede Suranjaya; Iriani Setyawati
SIMBIOSIS Vol 6 No 2 (2018)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (481.271 KB) | DOI: 10.24843/JSIMBIOSIS.2018.v06.i02.p05

Abstract

Ethanol can damage the liver, indicated by an increase of alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in the blood of alcohol-treated rats. Coffee is rich in antioxidants which are hepatoprotector agent. This study aimed to determine the effect of coffee to reduce alanin amino transaminase (ALT) activity and aspartat amino transminase (AST) activity in blood serum of alcohol-treated rats. The study used 32 male wistar albino rats (200±20g weight) which were divided into four treatment groups i.e. P0 (negative control which were treated with 1 mL of 30% ethanol/200g body weight (bw)/day), P1 (1 mL of 30% ethanol/200 g bw/day + coffee 0,1g/200g bw in 3,5 mL of water/200 g bw/day), P2 (1 mL of 30% ethanol/200g bw + coffee 0,15 g/200g bw in 3,5 mL of water/200g bw/day), dan P3 (1 mL of 30% ethanol/200g bw + coffee 0,2g/200g bw in 3,5 mL of water/200g bw/day). ALT and AST levels were measured by spectrophotometric method. Data analysis was done by ANOVA followed with LSD test and correlation analysis. The results showed that coffee reduced (P<0.01) the activities of ALT and AST of rats blood serum. ALT and AST activities were significantly different (P<0.01) among treatments and with control. Dose of coffee was negatively correlated with ALT activity with correlation coefficient (R) -0.978 and AST with correlation coefficient (R) -0.985 (P<0,01). The lowest of ALT and AST activities were found at dose of coffee 0.2 g/200g body weight. Keywords: ethanol, ALT, AST, coffee
VARIATION OF FINGERPRINT PATTERNS OF KAILINESE AND TORAJANESE TRIBES IN PALU AND SIGI REGENCY, CENTRAL SULAWESI Graciella Stevani Gulo; I Ketut Junitha; Iriani Setyawati
SIMBIOSIS Vol 8 No 2 (2020)
Publisher : Jurusan Biologi Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (787.006 KB) | DOI: 10.24843/JSIMBIOSIS.2020.v08.i02.p01

Abstract

A research on the variation of fingerprint patterns of Kailinese and Torajanese tribes have been done in Palu and Sigi Regency, Central Sulawesi. The aims of this research were to determine the variation of fingerprint patterns, Dankmeijer Index, Furuhata Index and Total Ridge Count of Torajanese and Kailinese. The population was divided into three groups, namely the Kaili Da'a (Kaili tribe which has phenotypic characteristics resembling the Australomelanesoid race), other Kaili groups and the Toraja tribe belonging to the Mongoloid race. The samples of fingerprints were taken by using the purposive sampling method with a total of 180 probandus, consisted of 60 people (30 males and 30 females) in each ethnic group. The tips of ten fingers were rubbed with paper that had contained 4B pencil rubbing, fingerprints were taken using clear tape and then the tape affixed on a probandus form. Data was analysed by Chi-square test and Student-t test. The fingerprint analysis showed that there were four fingerprint patterns found among the Kailinese and Torajanese tribes. The highest whorl frequency was found in the Torajanese at 50.17%, whereas Da'a and other Kaili groups had higher loop frequencies. Whorl type analysis showed simple whorl was the type most commonly found in all three populations. Furthermore, double loop whorl and the least frequency was the central pocket whorl. The Furuhata Index values ??for the Toraja, Kaili and Kaili Da'a tribes respectively were 102.73; 57,37; and 95.06 while the Dankmeijer Index values ??were 1.99; 5,61; and 2.77. The average of total ridge count in the Toraja, Kaili and Kaili Da'a tribes respectively were 134; 128.08 and 133.56.
Co-Authors Adevalentin Lesik AGUNG EKO CAHYA KUSUMA PUTRA Agustina, Tika Puri Alfonds Andrew Maramis Anak Agung Ketut Darmadi Andayani, Ni Kadek Prederika Sari Areningrat, Putu Ayutia Daud, Putri Deny Suhermawan Yusup Dwi Ariani Yulihastuti Ezra Oktavia Ambarita Fernando Andre Watung Fita Efi Rafelina Siallagan Futwembun, Fransiska FX. Sudaryanto Graciella Stevani Gulo Haryo Seto Wicaksono Hasmiati Hasmiati Helen J Lawalata Helen Joan Lawalata Hety Hartaty I Gede Agus Pradana Putra I Gede Wiranatha I Gusti Agung Ayu Suartini I GUSTI AYU MANIK ERMAYANTI I Gusti Ayu Manik Widhyastini I Gusti Ngurah Agung Dewantara Putra I Ketut Junitha I Made Sara Wijana I Wayan Wena Diartha Ida Ayu Ratih Purnama Dewi Ida Bagus Made Suaskara Inna Narayani Jaqueline E.M Tangkau Joko Wiryatno Kadek Ayu Trisna Yanti Kadek Mardika Kadek Mardika Kamagi, Decky LUH Puru ESWARYANTI KUSUMA YUNI Luh Putu Eswaryanti Kusuma Yuni MADE PHARMAWATI Martin Joni Mokosuli Yermia Samuel Musma Rukmana Ngurah Intan Wiratmini Ni Gusti Ketut Roni Ni Kadek Prederika Sari Andayani Ni Komang Frely Astuti Ni Made Estriana Pranadya Ni Made Suartini Ni Nyoman Wirasiti Ni Nyoman Wirasiti Ni Putu Adriani Astiti Ni Putu Ayu Dewi Wijayanti Ni Wayan Karolina Ni Wayan Sudatri Nonny Manampiring Nova L.I.M Ogi Nur Assiam Orbanus Naharia Retno Kawuri Revolson Mege Rolef Rumondor Rompas, Christny Ferdina Evie Ruth Ellisa Christiani Sambeka, Delvino SANG KETUT SUDIRGA Supit, Annely Suranjaya I .Gd Verawati I Y Roring Wiesye Nangoy Yohana Deni Suryati