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Evaluasi Beberapa Ekotipe Jarak Pagar (Jatropha curcas L.) untuk Toleransi Cekaman Kekeringan Lapanjang, Iskandar; Purwoko, Bambang S.; Hariyadi, ,; Budi R, Sri Wilarso; Melati, Maya
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy) Vol. 36 No. 3 (2008): Jurnal Agronomi Indonesia
Publisher : Indonesia Society of Agronomy (PERAGI) and Department of Agronomy and Horticulture, Faculty of Agriculture, IPB University, Bogor, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.78 KB) | DOI: 10.24831/jai.v36i3.1386

Abstract

Drought stress in crop  occurs due to imbalance between water supply and demand.  Crop responses to drought stress  depend on their ecotypes.  This study was aimed at examining the tolerance of the physic nut ecotype to drought stress in inceptisol. This study was conducted at Silviculture greenhouse, Forestry Faculty IPB, from September to December 2007. A Completely Randomized Design with two factors was used in the experiment, i.e. water content (80, 60, and 40 % of field capacity) and physic nut ecotypes (Palu, NTB, IP-A, and IP-P) The results showed that (1) drought reduced stem diameter (31.4%), root length (65.49%) and leaf area (72.73%), and decreased plant dry weight (74.83%),(2) tolerance level of the evaluated physic nuts on the drought stress were moderate tolerance (IP-1A, NTB,  Palu) and sensitive (IP- Pakuwon).   Key words:  physic nut, drought stress, tolerance
THE POTENTIAL OF CARDAMOM LEAF IN THE AGROFORESTRY SYSTEM: ESSENTIAL OIL YIELD AND 1.8-CINEOL CONTENT Octavia, Dona; Wijayanto, Nurheni; Budi, Sri Wilarso; Batubara, Irmanida; Suharti, Sri
Indonesian Journal of Forestry Research Vol. 11 No. 1 (2024): Indonesian Journal of Forestry Research
Publisher : Association of Indonesian Forestry and Environment Researchers and Technicians

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.59465/ijfr.2024.11.1.17-32

Abstract

Cardamom (Amomum cardamomum), the ‘Queen of spices’, is a native Indonesian spice, a type of potential biopharmaceutical currently prospective because of its high selling value, especially for its fruit, with various benefits and its wide use. So far, cardamom essential oil comes from the utilization of its fruit, but the production is more limited, unlike the sustainably produced leaves, therefore, its leaves have the potential to be developed. This study aims to analyze the essential oil yield and 1.8-cineol content of cardamom leaves essential oil (CLEO) grown in agroforestry systems. The CLEO was obtained by steam-water distillation, while the 1.8-cineol content was analyzed by gas chromatography–mass spectrometry (GC-MS). The experimental design employed was a Randomized Complete Block Design with three cropping patterns as a treatment and three doses of bokashi fertilizer as blocks. The results showed that the highest yield of CLEO was generated in the sengon + cardamom agroforestry pattern of 3.16%, and the highest content of 1,8-cineol in CLEO was generated in the sengon + cardamom + arrowroot agroforestry pattern of 47.23%, while the lowest yield of CLEO and the 1,8-cineol content were found in the monoculture pattern of 2,02% and 43.16% respectively. Agroforestry practices have the potential to increase 1,8-cineol content and the yield of essential oil in cardamom leaf, which will be prospective in forest management to support forestry multi-business and social forestry programs.
BACTERIA FROM ARBUSCULAR MYCORRHIZAL FUNGI SPORES Gigaspora sp. AND Glomus sp.: THEIR ANTAGONISTIC EFFECTS TOWARDS SOILBORNE FUNGAL PATHOGENS AND GROWTH STIMULATION OF Gigaspora sp. in vitro Budi, Sri Wilarso
BIOTROPIA Vol. 20 No. 1 (2013): BIOTROPIA Vol. 20 No. 1 June 2013
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2013.20.1.252

Abstract

Eight isolates bacteria were isolated from surface sterilized endomycorrhizal spores of Gigaspora sp and six isolates bacteria isolated from Glomus sp. The tests to  the antagonists against fungal pathogens and stimulation of endomycorrhizae hyphal development of Gigaspora sp  in vitro  showed that there were three.isolates (GG1, GG5 and GL3) that have the ability to inhibit the growth of pathogens and enhance the development of hyphae in vitro. Enzymatic activity test showed that there were seven isolates that produce cellulase and protease activity that is GG1, GG3, GG6, GG7, GL2, GL4 and GL6  
DNA BARCODE CHARACTERIZATION OF MISTLETOE INFESTATION IN TEAK CLONAL SEED ORCHARD (CSO) IN PADANGAN, EAST JAVA PROVINCE, INDONESIA Muttaqin, Zainal; Budi, Sri Wilarso; Wasis, Basuki; Corryanti, Corryanti; Siregar, Iskandar Zulkarnaen
BIOTROPIA Vol. 24 No. 2 (2017): BIOTROPIA Vol. 24 No. 2 August 2017
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2755.603 KB) | DOI: 10.11598/btb.2017.24.2.651

Abstract

For effective teak plantation management, early detection system in controlling teak mistletoe requires various basic information, including degree of infestation and accuracy of the species names. Mistletoe infestations in teak and mistletoe species name have been reported, but there are still problems in identifying the correct species or subspecies due to morphological similarity. The objective of this study was to clarify the species identity of hemi-parasitic mistletoe plants, which were found in teak Clonal Seed Orchard (CSO) in Padangan, East Java Province, Indonesia using DNA barcodes. Species identification of teak mistletoe based on leaf morphological characteristics and universal DNA barcode regions (i.e. matK and rbcL) were carried out. The results showed that the Canonical Discriminant Analysis (CDA) could differentiate Dendrophthoe pentandra and Macrosolen tetragonus based on leaf morphological characteristics. Variables having high correlation to distinguish both species were length of petiole, width of the widest leaf, number of secondary leaf veins, leaf base shape, aspect ratio, form factor and perimeter ratio of diameter. The results of DNA barcoding showed that the two DNA barcode regions presented good amplification and sequence results. Both DNA barcode regions successfully differentiated two species i.e. D. pentandra and M. tetragonus which belong to Loranthaceae family and have similar leaf morphological characteristics. Those regions were also able to identify Viscum articulatum and other species belonging to Santalaceae family. These results suggested that the two DNA regions could become recommended universal DNA barcode for identifying teak mistletoe.
JABON (Anthocephalus cadamba Roxb.) POTENCY FOR REMEDIATING LEAD (Pb) TOXICITY UNDER NUTRIENT CULTURE CONDITION Setyaningsih, Luluk; Setiadi, Yadi; Budi, Sri Wilarso; Hamim, Hamim; Sopandie, Didy
BIOTROPIA Vol. 25 No. 1 (2018): BIOTROPIA Vol. 25 No. 1 April 2018
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11598/btb.2018.25.1.712

Abstract

Information on metal adaptation in plants is essential to understand the mechanism of heavy metal remediation, including lead (Pb), mediated by forest plants in contaminated land. This study aimed to identify the adaptation mechanism of jabon (Anthocephalus cadamba Roxb.) seedlings to excessive lead levels based on the tolerance index of growth performance and lead transport to plant tissue. The seedlings were exposed to lead Pb(NO₃)₂​ at concentrations of 0, 0.5, 1, 1.5, 5, and 10 mM in nutrient culture for 15 days. The results showed that the tolerance index (TI) of the seedlings significantly decreased with Pb exposure up to 1.5 mM, although the TI values remained above 75%. All seedlings died at Pb concentrations of 5 mM and above. Lead accumulated in all parts of the seedlings, with the highest concentration found in the leaves (735.9 ppm) under 0.5 mM Pb exposure. Pb was transported to the upper portion of the seedlings, as indicated by a transport factor (TF) greater than 1. The results suggest that jabon can tolerate excessive Pb exposure up to 1.5 mM and has potential as a phytoremediator plant.
GENETIC VARIATION OF TEAK MISTLETOE (Dendrophthoe pentandra (L.) Miq.) BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS Muttaqin, Zainal; Budi, Sri Wilarso; Wasis, Basuki; Siregar, Iskandar Zulkarnaen; Corryanti, Corryanti
BIOTROPIA Vol. 27 No. 2 (2020): BIOTROPIA Vol. 27 No. 2 August 2020
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.519 KB) | DOI: 10.11598/btb.2020.27.2.1211

Abstract

Mistletoes are hemiparasitic macroparasite plants that interfere with trees and other wild plants in nutrient acquisition. As the plant has low leaf water potential, it draws water from teak wood tissues during the deciduous stage of the teak host, thereby killing the twigs and eventually the teak tree. Mistletoes are also key players in plant diversity. Therefore, the mistletoe population needs to be regulated not only as a parasite but also as a keystone species affecting biodiversity. Knowledge scarcity on the status of mistletoes includes their genetic variation. Hence, the purpose of this study is to analyze the level of genetic variation of teak mistletoe (Dendrophthoe pentandra) using RAPD markers. At the Padangan teak Clonal Seed Orchard (CSO), leaf samples were randomly collected from three layers of the mistletoe crown (upper, middle, and below) taken from five host teak trees randomly selected from each of the sub-observation measure plots (OMP). Four OMP units inside the observation sample plots (OSP) (n = 3, 50 × 50 m) at different levels of infestation (light, moderate, and heavy) were established. Analysis of genetic variation and genetic distance of mistletoes across different crown layers was conducted using RAPD markers. The leaf samples from crown layers UU (upper crown, subsection upper), UM (upper crown, subsection middle), and UB (upper crown, subsection below), including U (upper crown), had significantly greater genetic variation (He = 0.181–0.255) than those from the M layer (middle crown, He = 0.227) and the B layer (below crown, He = 0.114). Furthermore, the greatest genetic distance occurred between mistletoes in the UB and B crown layers (0.310), whereas the shortest genetic distance occurred between mistletoes of the UU and UM layers in the upper crown (0.038). Practical implications of the low genetic variation include the control of D. pentandra infestation by restricting its population so that the Perhutani State-Owned Forestry Enterprise can maintain damage levels below the economic threshold.
The Effectiveness of Arbuscular Mycorrhizal Fungi Seed Coating on the Sengon Seed Germination under Drought Stress Nur Buana, Mursalina; Budi, Sri Wilarso
Journal of Tropical Silviculture Vol. 16 No. 03 (2025): Jurnal Silvikutur Tropika
Publisher : Departemen Silvikultur, Fakultas Kehutanan dan Lingkungan, Institut Pertanian Bogor (IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/j-siltrop.16.03.199-207

Abstract

Global warming has caused drought, which is a limiting factor for tree growth. The effect of drought stress can be suppressed by utilizing Arbuscular Mycorrhizal Fungi (FMA) which can be applied through seed coating. The study aimed to determine the response of sengon seed germination to drought stress, the effectiveness of FMA inoculation on sengon seedlings, and the effectiveness of seed coating on sengon seed germination under drought stress. The research was conducted with a two-factor completely randomized design, namely drought stress and FMA inoculation. The results showed that drought stress reduced the germination of sengon seeds. Application of FMA seed coating can increase seed tolerance to drought stress but has no real effect at the 5% level. Inoculation of FMA through seed coating showed higher growth rate and root colonization than inoculation in the planting media, indicating that seed coating has a higher effectiveness in the mechanism of tolerance of sengon seedlings to drought stress.
Evaluation of early growth and aluminum tolerance in Leucaena leucocephala (Lam.) de Wit seedlings under acidic conditions as a recommendation for post-coal mining land rehabilitation Salim, Mohammad Agus; Sudrajat, Dede J.; Nugroho, Aditya; Indriani, Fitri; Rustam, Evayusvita; Maharani, Dewi; Rosdayanti, Henti; Wawangningrum, Hary; Efendi, Muhammad; Suhaendah, Endah; Mulyana, Ade Nendi; Wahyudi, Imam; Setyaningsih, Luluk; Budi, Sri Wilarso
Journal of Degraded and Mining Lands Management Vol. 13 No. 2 (2026)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2026.132.9979

Abstract

One of the problems with acidic soil is its high aluminum (Al) content, which can cause toxicity to plants. this study was conducted to evaluate the preliminary growth and tolerance of L. leucocephala seedlings to Al exposure. This study used a completely randomized design (CRD) with one factor, namely Al concentration, consisting of 5 levels (0, 2, 4, 6, and 8 mM). The evaluation of seedling growth and tolerance assessed included: height, root length, dry weight of roots and shoots, tolerance index, SPAD (Soil Plant Analysis Development) chlorophyll, and photosynthesis rate. The results showed that Al exposure reduced all growth parameters (height, root length, dry weight of roots and shoots) of L. leucocephala seedlings. The tolerance index of L. leucocephala seedlings also decreased significantly (up to 43.68%) with increasing Al concentration. Meanwhile, chlorophyll and photosynthesis rate parameters increased with Al exposure of 2-4 mM, but decreased with higher Al exposure (6-8 mM). The threshold value or sensitivity of L. leucocephala seedlings to Al exposure was less than two mM. The L. leucocephala species can also serve as an alternative for land rehabilitation with high Al content as post-coal mining land.
Response of Eucalyptus pellita and Eucalyptus deglupta seedling growth to aluminum exposure Salim, Mohammad Agus Salim; Setyaningsih, Luluk; Wahyudi, Imam; Budi, Sri Wilarso
Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (Journal of Natural Resources and Environmental Management) Vol 12 No 2 (2022): Jurnal Pengelolaan Sumberdaya Alam dan Lingkungan (JPSL)
Publisher : Pusat Penelitian Lingkungan Hidup, IPB (PPLH-IPB) dan Program Studi Pengelolaan Sumberdaya Alam dan Lingkungan, IPB (PS. PSL, SPs. IPB)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/jpsl.12.2.246-258

Abstract

Aluminum (Al) is one of the problems and is a factor inhibiting plant growth on soils with acidic pH. This study aims to examine the growth response of Eucalyptus pellita and Eucalypus Deglupta seedlings to Al exposure. This study used a completely randomized design (CRD) with one factor, where the Al concentration consisted of 5 levels, namely: 0 mM (control), 2 mM, 4 mM, 6 mM, and 8 mM with 3 repetitions each and each repetition consisted of 3 units of the plant. The results showed that Al treatment had a significant effect on the growth parameters of both plant types, except for the root dry weight parameter for E. deglupta. Increasing the Al concentration can reduce almost all growth parameters. The 4 mM Al concentration was able to increase the height growth, plant dry weight (roots and shoots), and tolerance index for the E. pellita species, while the Al 2 mM concentration was able to increase the root growth of E. deglupta plants. Almost all growth parameters for E. pellita species were higher than that of E. deglupta, except for the SPAD chlorophyll index. The concentrations of 6 mM and 8 mM were toxic to E. pellita and E. deglupta.
Co-Authors , Hariyadi AAboe B. Saidi Abdurrani Muin Adisti Permatasari Putri Hartoyo Aditya Nugroho Agustina Puspita Dewi Aji Nuralam Dwisutono Akhir, Jumadil Akhmad Wahyudi Al Muzahiq, Hizbullah Allaily Allaily Andi Sukendro Ani Kurniawati Arif Budi Setiawan Arifandi, Candra Pradana Arum Sekar Wulandari Atang Sutandi Bambang S. Purwoko Baskoro Rochaddi Basuki Wasis Bayu Winata, Bayu Bedah Rupaedah Berry Oktavianto Betty Purwati Cahyo Wibowo Cahyo Wibowo Ceng Asmarahman Christina, Fiona Corryanti . Corryanti Corryanti Dadan Mulyana Daniel Happy Putra Dede J. Sudrajat Dida Syamsuwida Didy Sopandie Dona Octavia DWI ANDREAS SANTOSA Dwi Puspasari Arum Ramadhani Elis Nina Herliyana Endah Retno Palupi Endah Suhaendah Endang Suhendang Eny Widajati Erdy Santoso Erdy Santoso Erlina Rahmayuni, Erlina Evayusvita Rustam, Evayusvita Fadhlurrahman, Muhammad Miftah Faisal Al Asad Fatimah Nur Istiqomah Febrina, Ayudia Fiona Christina Fitri Indriani Fransisca Yosina Regina Luturmas Gunawan Gunawan Hamim Hamim Harus, Johanes S Henti Rosdayanti, Henti Herman, Welly Heru Bagus Pulunggono Husna Husna Husna Husna Hutami Indah Pertiwi Ida Rosita Ida Rosita Ikbal . Ikbal Ikbal Imam Gozali Imam Wahyudi Indrawan, Imam Wahyudi Intan Fajar Kemala Irdika Mansur Irmanida Batubara Iskandar . Iskandar Iskandar Iskandar Iskandar Iskandar M. Lapanjang Iskandar Z Siregar ISKANDAR ZULKARNAEN SIREGAR Istomo . Iswandi Anas JAYANI, FARADILA MEI Jenny Rumondang Jenny Rumondang Jumadil Akhir Kartika Megawati Kartini Kramadibrata Kartini Kramadibrata Khiorunnisa Rizki Dwi Jayati Khoryfatul Munawaroh Kurniati Kurniati Lina Karlinasari Luluk Setyaningsih Maharani, Dewi Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maman Turjaman Maryani Fatimah Putri Hardhani Maya Melati MEITY SURADJI SINAGA MEITY SURADJI SINAGA Meli Nurdiani Mohammad Agus Salim Muhammad Efendi, Muhammad Muhammad Helmi Mulyana, Ade Nendi Muttaqin, Zainal MZ, Karina Nelly Fridayanti Noor F Mardatin NUNANG LAMAEK MAY Nur Buana, Mursalina Nurheni Wijayanto Octaverina, Shinta Kartika Panca Dewi MH Karti Prijanto Pamoengkas Puspitasari, Sri Putri Aurum Putri Aurum Rahmatia, Citra Rahmawati Rahmawati Rumondang, Amandita Lintang Sabti Indah Purwanti Safinah S. Hakim S Hakim Safinah Surya Hakim Salim, Mohammad Agus Salim, Mohammad Agus Salim Saridi Satriyas Ilyas Silitonga, Exsaudina Siti Hanna Ghaida Sri Muryati Sri Muryati Sri Muryati Sri Muryati Sudirman Yahya SUDIRMAN YAHYA Suharti, Sri Surono Tedi Yunanto Teuku Tajuddin Tirsa Eka Saputri Ulfah Juniarti Siregar Wahono Sumaryono Wawangningrum, Hary Yadi Setiadi Yadi Setiadi Yadi Setiadi Yan Eka Prasetyawati YENNI BAKHTIAR YENNI BAKHTIAR YENNI BAKHTIAR Zainal Muttaqin Zuhriansah, Alfi Laila