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All Journal International Journal of Public Health Science (IJPHS) Journal of Food and Pharmaceutical Science Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences Disease Prevention and Public Health Journal Pharmaciana: Jurnal Kefarmasian Media Farmasi : Jurnal Ilmu Farmasi (Journal Of Pharmaceutical Science) Majalah Obat Tradisional JOURNAL OF HEALTHCARE TECHNOLOGY AND MEDICINE INDONESIAN JOURNAL OF PHARMACY Jurnal Farmasi Sains dan Komunitas Jurnal Teknologi Laboratorium Jurnal Fitofarmaka Indonesia Jurnal Penelitian Pendidikan IPA (JPPIPA) Syntax Literate: Jurnal Ilmiah Indonesia Jurnal Kesehatan Manarang Jurnal Pemberdayaan: Publikasi Hasil Pengabdian Kepada Masyarakat Jurnal Ilmu Kefarmasian Indonesia Lumbung Farmasi : Jurnal Ilmu Kefarmasian Jurnal Ilmiah Ibnu Sina (JIIS): Ilmu Farmasi dan Kesehatan Jurnal Farmasi Sains dan Praktis Jurnal Farmasi Klinik dan Sains Journal of Public Health and Pharmacy Journal of Food and Pharmaceutical Sciences Medical Sains : Jurnal Ilmiah Kefarmasian Tamilis Synex: Multidimensional Collaboration
Nanik Sulistyani
Fakultas Farmasi, Universitas Ahmad Dahlan
Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience Nursing Physics Public Health Social Sciences Other

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Journal : INDONESIAN JOURNAL OF PHARMACY

 1 
The antiviral activity of srikaya seed (Annona squamosa L.) ethanolic extract against newcastle disease virus in chicken embryo Nanik Sulistyani; Ibnatul Azizah; M. Kuswandi
Indonesian Journal of Pharmacy Vol 20 No 2, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (207.358 KB) | DOI: 10.14499/indonesianjpharm0iss0pp62-67

Abstract

Srikaya (A. squamosa L.) is a plant containing polyphenol, flavonoid, tannin, alkaloid and saponin compounds. These compounds could be extracted by ethanol solvent. Previous study shown that infuse of Srikaya seed had antiviral activity with value of IC50 3,236 'g/mL against Newcastle Disease Virus (NDV). Therefore, the aim of research is to know the potency of ethanolic extract of Srikaya seed as antiviral against Newcastle Disease Virus.The 28 chicken embryos at age of 9-11 days were classified into 7 groups, i.e. : control of virus, control of solvent ethanol : aquadest (1:10) and treatment groups were given ethanolic extract of Srikaya seed with concentration 0.1 'g/mL; 0.15 'g/mL; 0.2 'g/mL; 0.25 'g/mL; dan 0.3 'g/mL. NDV was inoculated into allantoic cavity intermediate after inoculated of extract and then incubated for 2 days. Antiviral activity were observed by hemaglutination method. The value of IC50 was calculated by probit analysis.The result showed that ethanol extract of srikaya seed (A. squamosa L.) had antiviral activity because of increasing concentration caused the higher inhibition percentage of hemaglutination. The value of IC50 is 0.152 'g/mL.Key words: Antiviral, Annona squamosa, Newcastle Disease Virus (NDV).
THE CLEAVING ACTIVITY ASSAY ON SUPERCOILED DNA BY PROTEIN FRACTIONS FROM Morinda citrifolia LEAVES Nanik Sulistyani; Sismindari .; Sudjadi .
Indonesian Journal of Pharmacy Vol 13 No 4, 2002
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (56.316 KB) | DOI: 10.14499/indonesianjpharm0iss0pp174-179

Abstract

Study on supercoiled DNA cleaving activity assay of protein fraction from Morinda citrifolia leaf had been done. The aim of this research is to compare the supercoiled DNA cleaving abilities among the fractions. This is a preliminary screening to find RIP from Morinda citrifolia Fractionation of protein was done by adding Ammonium Sulphate up to 20%, 40%, 60%, 80% and 100% in the crude extract, saturated solution was then centifugated to get the pellet fractions and coded as F-20, F-40, F-60, F-80 and F-100. The mixture of pUC19 plasmid and protein fractions, in the same protein concentration were incubated at room temperature for one hour, the cleaving ability of protein fraction on supercoiled DNA.was investigated. The results indicated that protein fractions, of F-80 had the highest cleaving activity to supercoiled DNA.Key words : Ribosome-Inactivating Protein (RIP), supercoiled DNA , protein fraction, Morinda citrifolia.
The antiviral activity of srikaya seed (Annona squamosa L.) ethanolic extract against newcastle disease virus in chicken embryo Nanik Sulistyani
Indonesian Journal of Pharmacy Vol 20 No 2, 2009
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjpharm0iss0pp62-67

Abstract

Srikaya (A. squamosa L.) is a plant containing polyphenol, flavonoid, tannin, alkaloid and saponin compounds. These compounds could be extracted by ethanol solvent. Previous study shown that infuse of Srikaya seed had antiviral activity with value of IC50 3,236 'g/mL against Newcastle Disease Virus (NDV). Therefore, the aim of research is to know the potency of ethanolic extract of Srikaya seed as antiviral against Newcastle Disease Virus.The 28 chicken embryos at age of 9-11 days were classified into 7 groups, i.e. : control of virus, control of solvent ethanol : aquadest (1:10) and treatment groups were given ethanolic extract of Srikaya seed with concentration 0.1 'g/mL; 0.15 'g/mL; 0.2 'g/mL; 0.25 'g/mL; dan 0.3 'g/mL. NDV was inoculated into allantoic cavity intermediate after inoculated of extract and thenincubated for 2 days. Antiviral activity were observed by hemaglutination method. The value of IC50 was calculated by probit analysis.The result showed that ethanol extract of srikaya seed (A. squamosa L.) had antiviral activity because of increasing concentration caused the higher inhibition percentage of hemaglutination. The value of IC50 is 0.152 'g/mL.Key words: Antiviral, Annona squamosa, Newcastle Disease Virus (NDV).
Co-Authors . Mulyadi Abdi Wira Septama Abdul Rahman Wahid Abdul Wahid Suleman ACHMAD NURYADIN AKBAR Ade Irawan Ade Irawan Adnan, Adnan Akrom, Akrom Aldri Frinaldi Alfian Syarifuddin Alvionida, Fitra Andhika Septiawan Anisa Devi Kharisma Wibowo Anisaningrum Anisaningrum Anisaningrum, Anisaningrum Anisaningrum, Anisaningrum Aprilia Kusbandari Astryna, Syarifah Yanti Athifah Candra Dewi Ayu Lifia Nur Kartikasari Definingsih Yuliastuti Dhega Agung Wichaksono Dinda Anindya Sabillah Dyah Aryani Perwitasari Eka Kumalasari Eka Kumalasari Faisal Nur Arib Fauziyya, Riri Fitra Alvionida Fitri, Dwiki Ghina Adhila Henry Budiawan Prasetya Ibnatul Azizah Ichwan Ridwan Rais Iin Narwanti IIN NARWANTI Ken Zaim Arifin Kintoko Kintoko Kintoko, Kintoko La Malihi Laela Hayu Nurani Lalu Muhammad Irham Leswara, Dianita Febrina Lilies Kusuma Wardhani Lilies Kusuma Wardhani Lola Angelita Lukman Hardia Lukman Mahdi M. Kuswandi Mahdi, Lukman Maliza, Rita Ma’ruf, Muhammad Melati Aprilliana Ramadhani, Melati Aprilliana Meta Ayuni Mika Triza Misba Muhammad Fathurrachman Mantali Muhammad Syakbani Mulyadi, . Mulyadi, . Mutti, Annisa Aulia Nina Salamah Nining Sugihartini NUR HASANAH Nurhayati Nurhayati Nurkhasanah Mahfudh Nurkhasanah Mahfudh Nurkhasanah Mahfudh Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nurkhasanah Nyoman Rudi Kusuma Panji Ratih Suci Rahma Dona Rahmat Dani Satria Riat El Khair Ricke Desyratnaputri Rizky H. Mawardi Rofidah Nur Umar Sabrina Wulandari Sianu, Rahman S Sismindari . Sudjadi . Sun, Suny Tegar Prasetya Budi Titiek Suhardi Haripurnomo Kushadiwijaya Hidayati Wardhani, Lilies Kusuma Warsi Warsi Wirawan Adikusuma Wulandari, Sabrina Wulandari, Sabrina Zainab Zainab Zainab Zainab Zainab, Zainab