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Journal : INDONESIAN JOURNAL OF PHARMACY

DEVELOPMENT AND VALIDATION OF A HPLC METHOD FOR DETERMINATION AND QUANTIFICATION OF RUBRAXANTHONE IN STEM BARK EXTRACT OF MANGOSTEEN Meri Susanti; Dwisesaria Irma Lena; Dachriyanus Dachriyanus
Indonesian Journal of Pharmacy Vol 25 No 4, 2014
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (940.042 KB) | DOI: 10.14499/indonesianjpharm25iss4pp237

Abstract

Rubraxanthone is one of antibacterial  and antioxidant compound which is isolated from stem bark of mangosteen (Garcinia mangostana Linn.). The precise method for analysis of plant constituents is normally a reverse-phase high performance liquid chromatographic (RP-HPLC). Development and validation of a RP-HPLC method for chromatographic separation was carried out on a reversed-phase column Shimadzu ®  Shimp-pack VP – ODS (4.6x250mm) using an isocratic mobile phase of  0.4% formic acid - acetonitrile at  a flow rate 1mL min-1, and detection was  with a UV detector. The linearity of  the proposed method was found in the range of 2.5-25μg/mL-1 with regression coefficient 0.999. Intraday precision studies showed the relative standard deviation ≤1.58% and  inter-day ≤3.20%. Accuracy of the method was determined by a recovery study conducted at 3 different levels, and the average recovery was 102.18%. The Limit of Detection Limit of Quantitation (LOQ) and were 0.47 and 1.56μg/mL-1.The contents of rubraxanthon in the crude was 0.23±0.07%w/w.
Co-Authors Amatul Firdausa Nasa Andar Indra Sastra Anggreiny, Nila Angraini, Atika Ledia Annisa Fitriani, Annisa Anshori, Putra Anugrah, Raffli Dwi Ar, Rosmita Ariandi, M. Satriadi Aulia, Said Reza Bangun, Mentari Wahyunta Br Br Saragih, Zanariah Busihat, Busihat Cikita Aidha Putri Dachriyanus Dania, Ira Aini Destiniar Destiniar, Destiniar Dewi Pangestuti Diny Amenike Dira Hefni Dwi Puspasari Dwisesaria Irma Lena Efrisnal, Dodi Egha Febrianingsih Erlinda Erlinda Fahmy, Rahmi Fatma Sri Wahyuni Fatsiwi Nunik Andari Fitria Rahmi Hariko, Rezki Haznaz Dhiya Ulhaq Z.N. Hildayani Ibarra, Florante Isna Mulyani Izzanil Hidayati Julianita, Diani Kurniawati kusmiarti, reni Lady Diana Yusri lala septiyani sembiring, lala septiyani Liliyana Sari Lina, Liza Fitri Mafaza Mafaza Mamnun, Hairo Manurung, Mei Juita Mardha Akhsanita Maria Goretti Adiyanti Mayang Sari Ayu Meifal Rusli Nasril, Nasril Nasution, Halimah Thania Netrawati, Netrawati Neviyarni, Neviyarni Nila Anggreiny Nila Angreiny Ningtiyas, Meiga Puji Nita, Melda Nur Ahyani Oktaria, Selly Putra, Deddy Prima Putra, Deddy Prima Qolbi Khoiri Rahma, Dinda Saufia Rangkuti, Irmayanti Rizqillah Pasaribu, Saadatur Rozi Sastra Purna Sabiruddin, Sabiruddin Salsabila, Cantika Sari, Siti Kemala Septi Mayang Sarry Shasitharan Raman Kutty Silvi Yulia Sari Siska Oktari Sufyarma Marsidin Suhatri Suhatri Surya Akbar Sylvia Dewiriza Tiarnida Nababan Tri Rahayuningsih Wahyu Widodo Wahyu, Haifa Yantri Maputra Yeni Karneli Yohannes Alen Yosea Kurnianto