Ida Bagus Ngurah Swacita
Fakultas Kedokteran Hewan, Universitas Udayana

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Journal : Jurnal Veteriner

KAJIAN TENTANG BERAT RELATIF BEBERAPA ORGAN VISERAL ITIK BALI STUDY OF THE RELATIVE WEIGHT OF SEVERAL VISCERAL ORGANS OF BALI DUCKS Ida Bagus Swacita; I Wayan Suardana
Jurnal Veteriner Vol 7 No 4 (2006)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Pada dasarnya hasil pemotongan ternak itik dapat dikategorikan menjadi dua komponen yaitu komponen karkas dan non-karkas
Seroprevalensi Sistiserkosis pada Babi di Papua (SEROPREVALENCE OF PIG CYSTICERCOSIS IN PAPUA REGION) Ida Bagus Ngurah Swacita; I Ketut Suada; Ketut Budiasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; I Nyoman Polos; I Made Damriyasa
Jurnal Veteriner Vol 18 No 1 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (86.971 KB) | DOI: 10.19087/jveteriner.2017.18.1.18

Abstract

Pig cysticercosis is an infection caused by the larval stage of pork tapeworm and Papua is one of the largest endemic areas of cysticercosis in Indonesia. This survey aim was to determine the seroprevalence of pig cystisercosis in Papua. A total of 311 pig serum samples collected from six regencies in Papua were examined using Enzyme Linked Immunosorbent Assay (ELISA). The result of the survey showed that the average seroprevalence of pig cysticercosis in Papua was 23.5% (73/311), where the highest seroprevalence was found in the regency of Jayawijaya was 42.6% (43/101), Biak 22.5% (9/40), Nabire 20.6% (7/34), Mimika 17% (8/47), Jayapura 13.5% (5/37), and Merauke 1.9% (1/52). It can be concluded that the seroprevalence of pig cysticercosis in Papua is still high, therefore, it is necessary to do more intensive programs to prevent and control this disease. ABSTRAK Sistiserkosis pada babi adalah infeksi yang disebabkan oleh stadium larva cacing pita, dan Papua merupakan salah satu daerah endemis sistiserkosis di Indonesia. Tujuan penelitian ini adalah untuk menentukan seroprevalensi sistiserkosis pada babi di Papua. Sebanyak 311 sampel serum babi yang dikumpulkan dari enam kabupaten di Papua diuji dengan Enzyme Linked Immunosorbent Assay (ELISA). Hasil penelitian menunjukkan bahwa rataan seroprevalensi sistiserkosis pada babi di Papua sebesar 23,5% (73/311), dan seroprevalensi terbesar ditemukan di Kabupaten Jayawijaya 42,6% (43/101), Biak 22,5% (9/40), Nabire 20,6% (7/34), Mimika 17% (8/47), Jayapura 13,5% (5/37), dan Merauke 1,9% (1/52). Dapat disimpulkan bahwa seroprevalensi sistiserkosis pada babi di Papua masih tinggi, sehingga diperlukan program yang lebih intensif untuk mencegah dan mengontrol penyakit ini.
Studi Histologi dan Histomorfometri Daging Sapi Bali dan Wagyu (HISTOLOGYCAL AND HISTOMORPHOMETRY STUDY OF BALI CATTLE AND WAGYU BEEF) Ni Ketut Suwiti; I Putu Suastika; Ida Bagus Ngurah Swacita; I Nengah Kerta Besung
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The objective of this study was to detect histological structure and histomorphometry of bali cattleand wagyu beef. The samples were collected from rib eye muscle of wagyu and bali cattle. The tissuesamples were fixed, dehydrated and embedded in paraffin and 4-5 mm sections, for histological examinationand stained with Haematoxilin-Eosin (HE). The light binocular microscope was used to identify the samples.The study showed that the beef histological structure consisted of: cells / muscle fibers, fatty and connectivetissues. The muscle fibers of wagyu were observed larger, regular, and longer compared to the Bali cattle.Additionally, intramuscular fat cells and connective tissue interlobulus were found less and thinner thanthe Bali cattle. In the area of muscle where the longitudinal cut was done, its observed that the presence ofI and A bands in Bali cattle beef were clearer compared to the wagyu. The diameter muscle fibers of balicattle beef (45.00 ±1.94 ?m), was smaller (P < 0.01) compared to the wagyu (75.00±1.82 ?m), as well as fatcell size of bali cattle beef (90.10 ±2.13 ?m) and wagyu beef (195.00±2.57?m). In conclusion, the structureof histology and histomorphometry of rib eye muscle in Bali cattle were different to the wagyu.
Pelacakan Ekspresi Antigen Toxoplasma gondii secara Imuno(sito)histokimia (TRACKING EXPRESSION OF TOXOPLASMA GONDII ANTIGENS USING IMMUNO(CYTO)HISTOCHEMISTRY METHOD) Ida Ayu Pasti Apsari; Ida Bagus Oka Winaya; Tjokorda Sari Nindhia; Ida Bagus Ngurah Swacita
Jurnal Veteriner Vol 18 No 4 (2017)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (114.568 KB) | DOI: 10.19087/jveteriner.2017.18.4.535

Abstract

The research objective want to track the expression of Toxoplasma gondii antigenes in the heart of the free-range chicken with immuno(cyto)histochemistry technique. In the present study were examined three methods to track antigen T.gondii, first method direct microscopic examination of the heart digests, the second method to detect antigen of T.gondii with immunocytochemistry technique of the chicken heart digests and the third immunohistochemistry examination of the heart free-range chicken. The number of material samples examined were 100 heart free-range chicken. Direct microscopic examination of the heart digests free-range chicken to track the bradyzoite form (inside cyst). Examination by immuno(cyto)histochemistry technique keep track T.gondii an antigen expression on cells and heart muscle tissue. The results showed that the direct microscopic examination on the heart tissue unable to detect cyst and antigen T.gondii. Immunohistochemical examination successfully detected the expression of antigenes T.gondii and was found 2% (2/100) positive. It can be concluded that T.gondii antigen expression in the heart of range chicken could be detected by immunohistochemistry.
Produksi dan Karakterisasi Antibodi Monoklonal Anti-Cysticercus cellulosae (PRODUCTION AND CHRACTERIZATION OF MONOCLONAL ANTIBODIES AGAINST CYSTICERCUS CELLULOSAE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; Ida Bagus Made Oka; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 3 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

The purpose of this study is to make a monoclonal antibody against- Cysticercus cellulosae and itscharacterization. Samples antigen prepared from T. solium larvae (C. cellulosae) was then used to immunizeBalb/c. The immune response of mice assessed by ELISA test, then the lymphocytes of mice used for theproduction of monoclonal antibodies (MoAb). Origin lymphocytes of mice that produce antibodies againstC. cellulosae antigen, fused with myeloma cells (NS1). Results fusion of two cells produces hybrid cellscalled hybridomas; cells are then screened by ELISA test. Hybridoma cells that produce only MoAb, usedto produce large quantities in vitro. Characterization of MoAb against-C.cellulosae was tested by usingELISA and Western blotting. Mice were immunized with C.cellulosae antigen showed an immune responseproducing antibodies to C.cellulosae. Based on the results of fusion, produced a total of 51 hybridoma cellclones and after being screened, only three clones of hybridoma cells that produced MoAb against–C.cellulosae. MoAb produced, named after the hole where the growth of the ELISA micro plate, the BE6,BE7, and EE9. Characteristics of this MoAb capable of tracking cellulosae of fluid larvae and recognizeantigen protein bands with molecular weight 78kDa.
Respons Imun Mencit yang Diimunisasi dengan Cysticercus cellulosae (IMMUNE RESPONSE TO TAENIA SOLIUM CYSTICERCOSIS IN MICE) Ida Bagus Ngurah Swacita; I Made Damriyasa; Nyoman Sadra Dharmawan; Nyoman Mantik Astawa; Ida Ayu Pasti Apsari; I Wayan Masa Tenaya
Jurnal Veteriner Vol 16 No 2 (2015)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

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Abstract

Cysticercosis is a zoonotic parasitic disease which is still problem in Indonesia. The purpose of thisstudy was to investigate immune responses of mice that had been immunized using Taenia solium larval(Cysticercus cellulosae) antigens originated from infected pigs. Three kinds of the C.cellulosaeantigens,secretory and whole antigens were used to immunize three different groups Balb/c mice consisted of 15animals. The serum samples before and after immunization were tested with ELISA test. The resultsshowed that the third antigens induced highly significant titre (P<0,01)compared to unimmunized animals.However no significant different (P>0,05) were found when the third antigens were compared. It wasconcluded that immunization with the three kinds of C. cellulosae antigens can cause immunity in mice.
Co-Authors A.A. Ketut Agung Cahyawan W Agnes - Tasia Agung Denlira Sitepu Agustina A Naibaho Agustina Lesmauli Nazara Aida Lousie Tenden Rompis Aletha Yuliana Mandala, Aletha Yuliana Alvionita Alexther Lingga Anak Agung Gde Oka Dharmayudha Anak Agung Sagung Kendran Anastasya, Sindika Andriawino Berdionis Sanam Anggara Fajri Prasafitra Anjelia Martina Dewi Apriyani, Ester Muki Arta, I Komang Wira Kusuma Maha Christine Regina Fenita Wenno, Christine Regina Fenita DESKI CITRA DWITANIA Desy Ariani Ferdianti, Desy Ariani Dharmawan N.S Dhayanti, Ni Luh Evy DWI SURYANTO Elly Hariati Br Purba, Elly Hariati Br EMI HAMIDAH Gadis Ayu Septyawati Gracemon Loe Mau, Gracemon Gusti Ayu Yuniati Kencana Gusti Made Widyantara I Gede Mahardika I Gusti Agung Ayu Suartini I Gusti Made Krisna Erawan I Gusti Ngurah Sudisma I K. K. Agustina I Ketut Suada I Ketut Suada I Ketut Suada I MADE ADITYA SASTRAWAN I Made Damriyasa I Made Mahaputra I Made Ricky Dwi Cahya I Made Roby I Made Subrata I Made Sukada I Nengah Kerta Besung I NYOMAN MANTIK ASTAWA I Nyoman Polos I Nyoman Suarsana I Nyoman Suartha I Putu Suastika I Putu Sudiantara Cipta I Wayan Masa Tenaya I Wayan Polos I Wayan Suardana I Wayan Suardana I. A. P. Apsasri I. B. Ardana I.A.P. Apsari I.H. Utama I.W. Batan Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Pasti Apsari Ida Ayu Resmihariningsih Ida Bagus Made Oka Ida Bagus Oka Winaya Iolanda Hermenegildo da Costa Kade Karang Agustina Kadek Karang Agustina Ketut Budiasa Ketut Tono Pasek Gelgel Laras Ayu Nadira Leksana Aditya Kris Nugraha Lely Anggriani Nababan Luh Made Destriyana Luh Made Sudimartini Luh Putu Pradnya Swari M.D. Rudyanto Made Beratha Mukti Martha Putri Manullang Martin Pedro Krisenda Resman MAS DJOKO RUDYANTO Maulina Nababan, Maulina Mita Andini, Mita MURNI SARI Natalia Natalia Nelci Elisabeth Bolla Ni Kadek Deasy Pitriyawati Ni Ketut Suwiti Ni Made Chintya Dewi Sukasari Ni Made Dwi Alita Wulandari Ni Nyoman Citra Susilawati Ni Putu Tessa Arsaning Rahayu Ni Wayan Arya Utami, Ni Wayan Arya Ni Wayan Ayu Rukmini Ni Wayan Suryanadi Pinontoan Kersty Putri Nathania Priharyanthi, Luh Komang Ayu Puteri Purnama Layli, Purnama Putu Aditya Pratama Arta Putra Putu Ayu Sisyawati Putriningsih Putu Henrywaesa Sudipa Remontara, Al Afuw Niha Rima Nurmayani Romy Muhammad Dary Mufa Sabelina Fitriani sang gede purnama Satria Yanuwardani Setiawan Sianturi, Inti Sari Pati R U Sihombing, Vivi Ekatry Stelen, I Dewa Ketut Raeyadi Purnama Suci Wulandari Sumartono - Supriaty Hutasoit, Supriaty T. Sari Nindia Tama, Kevin Tri Tjokorda Sari Nindhia Vinensia Ghona Gani Vinensia Ghona Gani Wayan Tunas Artama Wieke Sri Juniartini Yuli Darmawan, Yuli