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Khaswar Syamsu
Department Of Agricultural Industrial Technology - Bogor Agricultural University Dramaga, Bogor, 16680
Aerospace Engineering Agriculture, Biological Sciences & Forestry Automotive Engineering Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Control & Systems Engineering Decision Sciences, Operations Research & Management Dentistry Earth & Planetary Sciences Economics, Econometrics & Finance Education Electrical & Electronics Engineering Energy Engineering Environmental Science Health Professions Immunology & microbiology Industrial & Manufacturing Engineering Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Nursing Physics Public Health Veterinary Other

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Journal : Microbiology Indonesia

 1 
The Process of Xylanase Production from Bacillus pumilus RXAIII-5 NUR RICHANA; TUN TEDJA IRAWADI; M. ANWAR NUR; ILLAH SAILAH; KHASWAR SYAMSU
Microbiology Indonesia Vol. 1 No. 2 (2007): August 2007
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (324.944 KB) | DOI: 10.5454/mi.1.2.6

Abstract

The optimum conditions for the growth of Bacillus pumilus RXAIII-5 (a potential xylanase producer) were sought, these included temperature, pH, aeration, and agitation of the culture batch. Afterwards a mathematical model based on the parameter of cultivation kinetics was formulated. At the same time, the rheology of the fluid used for bacterial cultivation in a bioreactor was studied. The data obtained was used for estimating the ‘scaling up’ of enzyme production. The results of the study indicate that the optimum condition for processing in 50 ml Erlenmeyer flask are used temperature of 35 oC (308oK), pH 7, and an agitation rate of 140 rpm. The highest xylanase activity and its specific activity are 297.132 U.ml-1 and 655.32 U.g-1protein, respectively. Subsequent experiments in a bioreactor using all of the experiment parameters mentioned above, except for the agitation rate, shows that the results are as follows. The highest specific growth was at 0.082 hour-1 at an aeration and agitation rate of 0.5 vvm and 150 rpm, respectively. Based on the data of the cultivation kinetics, the optimum conditions for the fermentation in Biostat 2L-bioreactor is 1 vvm and 200 rpm of aeration and agitation, respectively . The efficiency of substrate (Yp/s) and of cell biomass (Yp/x) to produce xylanase is 50.744 U.g-1 and 43.906 U.g-1, respectively. The efficiency of substrate to cell production (Yx/s) is 1.178g.g-1. The liquid cultivation-medium has non-Newtonian properties. Based on a mathematical model it is found that the consistency index (k constant) and index of liquid behavior (n value) are 0.179 g.cm-1.second-1 and 0.3212, respectively. Becouse the value of 0
Selection of Carbon and Nitrogen Source for 8-Hydroxy 9, 12-Octadecadienoic Acid Production using Endophytic Fungi Curvularia lunata BioMCC FE-00283 ERWAHYUNI ENDANG PRABANDARI; TUN TEDJA IRAWADI; WAHONO SUMARYONO; KHASWAR SYAMSU
Microbiology Indonesia Vol. 5 No. 4 (2011): December 2011
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (84.109 KB) | DOI: 10.5454/mi.5.4.6

Abstract

Hydroxyoctadecadienoic acid (HODE) is one of hydroxy fatty acids that has anticancer activity.  HODE was previously produced by chemical synthesis or bioconversion from linoleic acid. This is the first paper reported production of HODE by Curvularia lunata an endophytic fungi of Cibotium barometz. Various carbon and hydrogen sources have been tested for their effects on the production of  HODE by C. lunata. Glucose, lactose, maltose, xylose, and sucrose were used as carbon sources, while yeast extract, monosodium glutamate, urea, and NH4Cl were used as nitrogen sources. Fermentation was done using 100 ml medium in 250 ml Erlenmeyer flask at 150 rpm, 28 oC for 10 days.  HODE products were analyzed by high pressure liquid chromatography using C18 coloumn and eluted by gradient system of acetonitril-water from 15% to 100%. Glucose  and monosodium glutamate were found to be the best carbon and nitrogen source.  The optimum concentration of glucose and monosodium glutamate for the production of HODE were 10 mg L-1 and 12 mg L-1 respectively.
Medium Optimization for Penicillin Acylase (PAc) Production by Recombinant B. megaterium MS941 Containing pac Gene from B. thuringiensis BGSC BD1 Using Response Surface Methodology FENTRI PARAMITHA PUTRI; ASTUTIATI NURHASANAH; NIKNIK NURHAYATI; IS HELIANTI; KHASWAR SYAMSU
Microbiology Indonesia Vol. 9 No. 2 (2015): June 2015
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1794.751 KB) | DOI: 10.5454/mi.9.2.3

Abstract

Penicillin G acylase (PAc) hydrolyses of the amide bond of benzylpenicillin (Pen-G) releasing PAA and 6-APA, key intermediate in the production of various semisynthetic penicillins. In this study, we optimised the production medium of PAc by RSM using two variables (xylose as inducer and CaCl2 as divalent cations) to obtain the optimum PAc specific activity from Bacillus megaterium btpacBD1. For this purpose, combinations of five different xylose concentrations (0.13 – 0.87 %) and five different CaCl2 concentrations (0.64 – 4.36 mM) were analysed, in a total of 22 experiments. CCD used for the analysis showed that in shake flask cultivations, xylose and CaCl2 showed significant effects on PAc volumetric activity and the quadratic model was in good agreement with the experimental results (R2= 0.86 (p-value < 0.0001)). The maximum specific activity (130.669 ± 50.241 units mg protein-1) was reached when xylose and CaCl2 concentrations were 0.49% and 2.4 mM, respectively, and medium pH was around 7. Under such conditions, the activity of PAc and protein concentration achieved were 1.318 ± 0.406 units mL-1 and  0.0101 ± 0.01 mg mL-1. The shake flask validation experiments demonstrated that with such medium composition the volumetric activity, protein concentration and specific activity achieved were 1.294 ± 0.171 units mL-1, 0.0102 ± 0.0003 mg mL-1 and 125.91 ± 13.309 units mg-1, respectively. When the optimum medium composition was applied in 10 L bioreactor, the optimum volumetric activity (2.0687 ± 0.0820 units mL-1) and protein concentration (0.0078 ± 0.0008 mg mL-1) were achieved 48 h after the start of the cultivation. However, the optimum PAc specific acivity (1260.52  ± 27.5711 units mg protein-1) was achieved 18 h after the start of the cultivation.
Co-Authors . Gunawan . Gunawan A. Aziz Darwis A. Herryandie Abdul Aziz Darwis Adhi Kusnadi Akhmad Endang Zainal Hasan Anas Miftah Fauzi Anggun Rahman Rahman Ani Suryani Ani Suryani Aninda Sekar ZAHRA Anwar Nur Anwar Nur Ardani Lukman ASEP RISWOKO ASTUTIATI NURHASANAH Bambang Prasetya Bambang Prasetya Bambang Prasetya Bambang Prasetya Bustomi - Bustomi Darwis, Abdul Aziz Dede Rais Diana Dewi Diana Dewi, Diana Diana Nurani Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi - Susilaningsih Dwi Purnomo Dwi Purnomo Dwi Setyaningsih Dyah Noor Hidayati DYAH NOOR HIDAYATI E. Gumbira Sa’id E. Gumbira-Sa’id E. Mardliyati Elisa Julianti Endang Gumbira Said Endang Warsiki Endang Warsiki dan Citra Dewi Wahyono Putri (E-Jurnal Agro-Industri Indonesia) Eni Dwi Islamiati Eni Dwi Islamiati Eni Dwi Islamiati, Eni Dwi Erliza Hambali Erliza Noor Erwahyuni E Prabandani ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari Erwahyuni Prabandari, Erwahyuni Essa Annisa Syadiah Etik Mardliyati Etik Mardliyati Etik Mardliyati, Etik Etty Riani FARAH FAHMA FENTRI PARAMITHA PUTRI Fitriani Kasim Gamma Irca Pradikta Griselda Happy Ramadhani Gumbira-Sa'id, E Gustri Yeni Gustri Yeni Han Roliadi Hanifah FUADI Hanny Hafiar Harahap, Serarifi Elagin Hendri Muchtar Hendri Muchtar, Hendri Hendri Ropingi Herastuti Sri I Nyoman Jaya Wistara Iftachul Farida Ika Amalia Kartika Ike Sitoresmi M Purbowati Illah Sailah Irawadi Jamaran Irvan Setiadi Kartawiria Irvan Setiadi Kartawiria IS HELIANTI Isroi Isroi Isroi Jayawarsa, A.A. Ketut Joko Santoso Kasid, Sri Kaidah kusnadi, adhi Lanjar Sumarno Lanjar Sumarno Lestari, Puji Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M Irfani ABDULLAH M. ANWAR NUR M. Daud Machfud Mala Nurilmala Manalu, Lamhot Mardliyati, Etik Muhammad Arif Darmawan Muhammad Daud Muhammad Rasyid RAMADHAN Muhammad Tasrif Muhammad Tasrif Mulyorini Rahayuningsih Murdiyatmo, Untung Muslich Muslich, Muslich N. A. Evalia Nabilah, Siti Nadia STEPANI Nastiti Siswi Indrasti Nasution, Nurul Cinthiya nFN Misgiyarta NIKNIK NURHAYATI Ninik Purbosari Nur Atifah Nur Richana Nurika Asih SETIYANI Nusaibah - Nusaibah Prayoga SURYADARMA Ridwansyah Ridwansyah Rini Purnawati Rofiq Sunaryanto Rofiq Sunaryanto S Heris Anita, S Heris S Joni Munarso Sa’id, E. Gumbira Sapta Raharja Sarifah Nurjanah Selvia Mahilda RIDHOHA Shinta PERMATASARI Siti Nabilah Sony Hartono Wijaya Sudirman Sudirman Suharyanto Suprihatin Suprihatin Surono SURONO Tajuddin Bantacut Taufik Hidayat Titi Candra Sunarti Tri Panji Tun T. Irawadi Tun Tedja Irawadi Tun Tedja Irawadi Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya FATRIASARI Widya Fatriasari Yandra Arkeman Yandra Arkeman Yandra Arkenan Yeni, Gustri Yeni, Gustri