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Direct Ethanol Production from Breadfruit Starch (Artocarpus communis Forst.) by Engineered Simultaneous Saccharification and Fermentation (ESSF) using Microbes Consortium Farida, Iftachul; Syamsu, Khaswar; Rahayuningsih, Mulyorini
International Journal of Renewable Energy Development Vol 4, No 1 (2015): February 2015
Publisher : Center of Biomass & Renewable Energy, Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/ijred.4.1.25-31

Abstract

Breadfruit (Artocarpus communis Forst.) is one of sources for ethanol production, which has high starch content (89%). Ethanol production from breadfruit starch was conducted by Simultaneous Saccharification and Fermentation (SSF) technology using microbes consortium. The aim of the research was to examine a method to produce ethanol by SSF technology using microbes consortium at high yield and efficiency. The main research consisted of two treatments, namely normal SSF and enginereed SSF. The results showed that normal SSF using aeration and agitation during cultivation could produce ethanol at 11.15 ± 0.18 g/L, with the yield of product (Yp/s) 0.34 g ethanol/g substrate; and yield of biomass (Yx/s) 0.29 g cell/g substrate, respectively. A better result was obtained using engineered SSF in which aeration was stopped after biomass condition has reached the end of the exponential phase. The ethanol produced was 12.75 ± 0.04 g/L, with the yields of product (Yp/s) 0.41 g ethanol/g substrate, and the yield of cell (Yx/s) 0.09 g cell/g substrate.
PRODUKSI BIOETANOL DARI BAGAS SORGUM MANIS MELALUI SAKARIFIKASI DAN FERMENTASI SIMULTAN (SSF) KONVENSIONAL MENGGUNAKAN Trichoderma reesei dan Saccharomyces cerevisiae Syadiah, Essa Annisa; Syamsu, Khaswar
EDUFORTECH Vol 6, No 2
Publisher : Program Studi Pendidikan Teknologi Agroindustri, UPI (Universitas Pendidikan Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17509/edufortech.v6i2.39289

Abstract

Bagas sorgum manis (Sorghum bicolor L. Moench) merupakan hasil samping industri gula dari nira sorgum manis yang dapat digunakan sebagai bahan baku pembuatan bioetanol. Tujuan dari penelitian ini adalah untuk memproduksi bioetanol dari bagas sorgum manis menggunakan Trichoderma reesei dan Saccharomyces cerevisiae. Teknik Sakarifikasi dan Fermentasi Simultan (SSF) konvensional adalah teknik SSF produksi bioetanol dengan aerasi penuh atau tidak direkayasa. Penggunaan teknik SSF memiliki kelebihan jika dibandingkan dengan teknik sakarifikasi dan fermentasi terpisah (SHF), yaitu waktu proses produksi yang dibutuhkan lebih pendek, meningkatkan rendemen, dan laju produksi bioetanol. Produk bioetanol akhir yang dihasilkan sebesar 6.60±0.28 g L-1. Dengan μx maks gabungan sebesar 0.02/jam, nilai Yp/s sebesar 0.17±0.01 g bioetanol/g substrat, dan laju produksi bioetanol sebesar 0.09±0.00 g L-1 jam-1.
Kajian perbaikan mutu pada agroindustri skala mikro dan kecil gambir indonesia Endang Gumbira Said; Khaswar Syamsu; A. Herryandie; E. Mardliyati; N. A. Evalia
Jurnal Ilmu Pertanian Indonesia Vol. 15 No. 2 (2010): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Quality is an absolute requirement used by product to compete in the global market. Therefore, as the competition is getting more tight , high-quality gambier products become an absolute demands that must be met. One of the steps that must be taken is continuous technological improvement. In this study, quality analysis of various raw gambier samples of Lima Puluh Kota regency and the Padang City in West Sumatera, and Musi Banyu Asin regency in South Sumatra was done. The results of dimensional measurement and weighing samples of Bootch Gambier, Lumpang Gambier, Wafer Block and Stick Gambier showed that the quality of raw gambier were very various. The variation occured because the drying process and storage of gambier were not good enough. The proximate analysis data showed, in terms of ash content, only two types of Gambier samples (Bootch CVR Gambier and Wafer Block CVR Gambier) that met quality requirements, whereas the Coin Gambier and Bootch CVA Gambier had a very high ash content (38.93 percent and 75.64 percent).
Peran PEG 400 dalam pembuatan lembaran bioplastik polohidroksialkanoat yang dihasilkan oleh Ralstonia eutropha dari Substrat Hidrolisat Pati Sagu Khaswar Syamsu; Liesbetini Hartoto; Anas Miftah Fauzi; Ani Suryani; Dede Rais
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The purpose of the research was to investigate the effects of PEG 400 addition on the characteristics of bioplastic polyhydroxyalkanoates (PHA). PHA was obtained by cultivating Ralstonia eutropha on hydrolysed sago starch substrate using fed batch method for approximately 96 hours. The biomass concentration obtained was 4 g/L with PHA yield 20-30% of dry cell weight. The bioplastic was formed with solution casting method in which chloroform was used as solvent and PEG 400 was used as plasticizer. The concentrations of PEG 400added were 10, 20, and 30% (w/w), respectively. Bioplastic properties which were tested were tensile strength, elongation to break, density, thermal properties, cristalinity, and functional group. The addition of plastisizer tend to increase tensile strength and elongation to break, but decrease density, cristalinity and melting point. Bioplastic with 30% PEG 400 addition gave the best results. Bioplastic with 30% PEG 400 gave a value of tensile strength of 0.083 MPa; elongation to break of 0.881%; density of 0.7881 g/cm3;  melting point of 158.95 ac; and cristalinity of 44.58%. With these properties, the resulted bioi plastic may be used for surgical strings. Keywords: Bioplastic, Polyhydroxyalkanoates (PHA), Ralstonia eutropha, hidrolysed sago starch, PEG 400
Penggandaan Skala Produksi Bioinsektisida Bacillus thuringiensis var. israelensis untuk Membasmi Jentik Nyamuk Aedes aegypti Mulyorini Rahayuningsih; Khaswar Syamsu; Abdul Aziz Darwis; Rini Purnawati
Jurnal Ilmu Pertanian Indonesia Vol. 12 No. 2 (2007): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

The objective of this research is to study the scaling up of bionsecticide production from Bacillus thuringiensisvar. israelensisusing onggok (a cassava by-product) as a carbon source. The insecticide produced was used to eradicate Aedes aegypti larvae. The product was a crystal protein produced during bacterial sporulation. Scaling up from laboratory to pilot plant scale was done using two methods, i.e. constant agitation power per unit volume (Pg/V) and constant oxygen transfer coefficient (kLa). The results showed that yield of product per substrate (Yp/s) of Pg/V based product with the value of 3.52 ± 0.02 spora per gram substrates was higher than Yp/s of  kLa based product with the value of 2.96 spora per gram substrate.  Logarithmic value of viable spore count (log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as compared to 7.17 ± 0.20 for kLa based product. Substrate efficiency was also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of Pg/V based product was lower (0.49 μg/ml) meaning that it was more toxic than kLa based product (0.62 μg/ml). Amino acid content of Pg/V based product was also higher than kLa based product. Constant Pg/V method was suggested as a based on the scaling up of bioinsecticide production of B. thuringiensis israe/ensison industrial scale. Keywords: bioinsecticide, Bacillus thuringiensisvar. israelensis, kLa, Pg/V, LC50, viable spore count
Campuran Pulp Tandan Kosong Kelapa Sawit dan Selulosa Mikrobial Nata de Cassava dalam Pembuatan Kertas Khaswar Syamsu; Liesbetini Haditjaroko; Gamma Irca Pradikta; Han Roliadi
Jurnal Ilmu Pertanian Indonesia Vol. 19 No. 1 (2014): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

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Abstract

Nowadays, forest-extracted wood cellulose still predominantly serves as raw material for pulp and paper manufacture in Indonesia. Consequently, the deforestation rate has alarmingly increased. The purpose of this research is to study the use of mixed microbial cellulose pulp from nata de cassava and pulp from empty oil palm fruit bunch (EOPFB) for paper manufacture. In this research, pulp of nata de cassava’s microbial cellulose was mixed with EOPFB pulp at various proportions. Additives (i.e. 2% alum, 2.5% tapioca starch, and 5% kaolin) were added to such mixture. Sheet forming without additives was made as a control. Addition of microbial cellulose tends to increase the physical and strength properties of paper and decrease the capability to absorb water significantly. The mixture of pulp of empty oil palm fruit bunch and microbial cellulose from nata de cassava has a potency to substitute wood pulp for paper manufacture.
PENGARUH PENAMBAHAN POLIOKSIETILEN-(20)-SORBITAN MONOLAURAT PADA KARAKTERISTIK BIOPLASTIK POLI-HIDROKSIALKANOAT (PHA) YANG DIHASILKAN Ralstonia eutropha PADA SUBSTRAT HIDROLISAT PATI SAGU Khaswar Syamsu, Chilwan Pandji, dan Eva Rosalina Lumbanraja
Jurnal Teknologi Industri Pertanian Vol. 18 No. 1 (2008): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRACTPoly-hydroxyalkanoates (PHA) were blended in solution with Polyoxyetilene-(20)-sorbitan monolaurate (Tween20). In presence of Tween20 component, it caused some polymer interaction between PHA and Tween20 component. Shifting of density, tensile strength and elongation at break was observed for PHA/Tween20 blend. Improvement in elongation at break was drastic in case of PHA blend with 5% Tween20. The addition of Tween20 can reduce PHA melting point and degree of crystallinity. The amount of crystalline was lower for PHA/Tween20 blend than PHA without Tween20 component. FTIR analysis proved that the alteration of PHA characteristic was caused by hydrogen bonding between PHA and Tween20 molecules. The highest blending performance based on its flexibility was showed by PHA blend with 5% Tween20.Keywords : Bioplastic, polyhydroxyalkanoates, Ralstonia eutropha, Tween20
KAJIAN PENGARUH KONSENTRASI H2SO4 DAN SUHU REAKSI PADA PROSES PRODUKSI SURFAKTAN METIL ESTER SULFONAT (MES) DENGAN METODE SULFONASI Khaswar Syamsu, Ani Suryani dan Nunung D. Putra
Jurnal Teknologi Industri Pertanian Vol. 14 No. 2 (2004): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRACTSurfactant (surface active agent) is usually used in cosmetics, laundry, and drugs industry. It's usually made from petroleum which is non renewable resources while the surfactant is non-biodegradable.  The process of making surfactant from petroleum also produces high pollution. Oleochemical is one of the alternative resources for producing surfactant which be able to cover the lacks of petroleum. Methyl Ester Sulfonat (MES) is surfactant made from palm methyl ester by sulfonation reaction with sulfuric supplier agents, i.e. sulfuric acid. The aim of this research is to obtain the best condition of sulfuric acid concentration and reaction temperature of sulfonation process in methyl ester sulfonate production from palm methyl ester.The best condition for the process is performed at concentration of sulfuric acid 80% and 65 oC of reaction temperature. It produces surface tension 32,80 dyne/cm, reduction of surface tension 47,10%, interfacial tension 0,65 dyne/cm, reduction of interfacial tension 98,16%, emulsion-stability 63,32%, but has the lowest L value 57,11 in scale 0 to 100.Key words :
KAJIAN PRODUKSI BIOINSEKTISIDA DARI Bacillus thuringiensis subsp israelensis PADA MEDIA TAPIOKA Abdul Aziz Darwis, Khaswar Syamsu dan Ummi Salamah
Jurnal Teknologi Industri Pertanian Vol. 14 No. 1 (2004): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRACTBacillus thuringiensis subsp israelensis (B.t.i)  as one  of the Bacillus thuringiensis  (B.t) species produces crystal protein  called delta endotoxin. As other B.t  crystal protein, this crystal  is specifically toxic to mosquitoes and blackflies.  This research  of B.t.i fermentation  used tapioca as carbon source and urea as nitrogen source.  The composition of media fermentation used were 0.5%, 1.0%, 1.5%, 2.0%, 2.5% and 3.0% of  tapioca, and 1% urea.  The result shows that B.t.i was able to grow in all treatment units.  Bioassay shows that the best performance was  obtained from media with 2.0% tapioca.Key words: Bacillus thuringiensis, tapioca
PRODUKSI BIOETANOL DARI HIDROLISAT PATI SINGKONG RACUN DENGAN FERMENTASI REPEATED-BATCH OLEH Saccharomyces cerevisiae TERIMOBILISASI PADA AMPAS SINGKONG Liesbetini Haditjaroko, Khaswar Syamsu, Anya Meryandini, Ahmad Jaelani Manurung TIP
Jurnal Teknologi Industri Pertanian Vol. 24 No. 1 (2014): Jurnal Teknologi Industri Pertanian
Publisher : Department of Agroindustrial Technology, Bogor Agricultural University

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Abstract

ABSTRAK Produksi bioetanol secara curah oleh sel mikroba bebas memiliki kelemahan, yaitu produktivitas dan efisiensinya yang rendah. Dalam penelitian ini sel Saccharomyces cerevisiae diimobilisasi menggunakan ampas singkong. Bahan ini merupakan hasil samping produksi pati singkong dan belum dimanfaatkan secara optimal. Penelitian ini bertujuan mengkaji proses produksi bioetanol dari pati singkong racun dengan fermentasi secara repeated-batch 6 siklus oleh S.cerevisiae terimobilisasi pada ampas singkong. Metode penelitian ini terdiri atas ekstraksi pati, pembuatan hidrolisat pati secara enzimatis, perlakuan terhadap ampas singkong oleh HCl 3% (v/v), imobilisasi S.cerevisiae pada ampas singkong, serta fermentasi secara repeated-batch. Pada penelitian ini diperoleh rendemen pati sebesar 26,20% (b/b) serta efisiensi konversi pati menjadi hidrolisat pati sebesar 36,89% (b/b). Penggunaan ampas singkong hasil perlakuan oleh HCl telah mampu mengimobilisasi sel sebanyak 1,76x1011 sel/g carrier. Fermentasi secara repeated-batch oleh S.cerevisiae terimobilisasi pada ampas singkong dengan kadar total gula awal 140 g/L telah menghasilkan etanol berkadar 42,72-63,66 g/L, produktivitas 1,78-2,66 g/L/jam, yield sebesar 0,33-0,47 g etanol/g gula, persentase konversi gula sebesar 90,80-95,74%, serta efisiensi sebesar 65,91-93,13%. Hasil penelitian ini menunjukkan bahwa metode imobilisasi yang digunakan memiliki kestabilan yang baik, serta didapatkan produktivitas bioetanol, persentase konversi gula dan efisiensi yang tinggi dengan fermentasi secara repeated-batch. Kata kunci: ampas singkong, bioetanol, fermentasi, repeated-batch, Saccharomyces cerevisiae
Co-Authors . Gunawan . Gunawan A. Aziz Darwis A. Herryandie Abdul Aziz Darwis Adhi Kusnadi Adhi Kusnadi Akhmad Endang Zainal Hasan Anas Miftah Fauzi Anggun Rahman Rahman Ani Suryani Ani Suryani Aninda Sekar ZAHRA Anwar Nur Anwar Nur Ardani Lukman ASEP RISWOKO ASTUTIATI NURHASANAH Bambang Prasetya Bambang Prasetya Bambang Prasetya Bambang Prasetya Bustomi - Bustomi Darwis, Abdul Aziz Dede Rais Diana Dewi Diana Dewi, Diana Diana Nurani Djumali Mangunwidjaja Djumali Mangunwidjaja Djumali Mangunwidjaja Dwi - Susilaningsih Dwi Purnomo Dwi Purnomo Dwi Setyaningsih DYAH NOOR HIDAYATI Dyah Noor Hidayati E. Gumbira Sa’id E. Gumbira-Sa’id E. Mardliyati Elisa Julianti Endang Gumbira Said Endang Warsiki Endang Warsiki dan Citra Dewi Wahyono Putri (E-Jurnal Agro-Industri Indonesia) Eni Dwi Islamiati Eni Dwi Islamiati Eni Dwi Islamiati, Eni Dwi Erwahyuni E Prabandani ERWAHYUNI ENDANG PRABANDARI ERWAHYUNI ENDANG PRABANDARI Erwahyuni Prabandari Erwahyuni Prabandari, Erwahyuni Essa Annisa Syadiah Etik Mardliyati Etik Mardliyati Etik Mardliyati, Etik Etty Riani FARAH FAHMA FENTRI PARAMITHA PUTRI Fitriani Kasim Gamma Irca Pradikta Griselda Happy Ramadhani Gumbira-Sa'id, E Gustri Yeni Gustri Yeni Han Roliadi Hanifah FUADI Hanny Hafiar Hendri Muchtar Hendri Muchtar, Hendri Herastuti Sri I Nyoman Jaya Wistara Iftachul Farida Ika Amalia Kartika Ike Sitoresmi M Purbowati Illah Sailah Irawadi Jamaran Irvan Setiadi Kartawiria IS HELIANTI Isroi Isroi Isroi Jayawarsa, A.A. Ketut Joko Santoso Kasid, Sri Kaidah Lanjar Sumarno Lanjar Sumarno Lestari, Puji Liesbetini Haditjaroko Liesbetini Hartoto Lucky Risanto M Irfani ABDULLAH M. ANWAR NUR M. Daud Mala Nurilmala Manalu, Lamhot Mardliyati, Etik Muhammad Arif Darmawan Muhammad Daud Muhammad Rasyid RAMADHAN Muhammad Tasrif Muhammad Tasrif Mulyorini Rahayuningsih Murdiyatmo, Untung Muslich Muslich, Muslich N. A. Evalia Nabilah, Siti Nadia STEPANI Nastiti Siswi Indrasti nFN Misgiyarta NIKNIK NURHAYATI Ninik Purbosari Nur Atifah Nur Richana Nurika Asih SETIYANI Nusaibah - Nusaibah Prayoga SURYADARMA Ridwansyah Ridwansyah Rini Purnawati Rofiq Sunaryanto Rofiq Sunaryanto S Heris Anita S Joni Munarso Sa’id, E. Gumbira Sapta Raharja Sarifah Nurjanah Selvia Mahilda RIDHOHA Shinta PERMATASARI Siti Nabilah Sony Hartono Wijaya Sony Hartono Wijaya Sudirman Sudirman Suharyanto Suprihatin Suprihatin Surono SURONO Tajuddin Bantacut Taufik Hidayat Titi Candra Sunarti Tri Panji Tun T. Irawadi Tun Tedja Irawadi Tun Tedja Irawadi Wahono Sumaryono Wasrin Safii Wasrin Syafii Widya Fatriasari Widya FATRIASARI Widya Fatriasari Yandra Arkeman Yandra Arkeman Yandra Arkenan Yeni, Gustri Yeni, Gustri