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All Journal Jurnal Sain Veteriner Indonesian Journal of Tropical and Infectious Disease Jurnal Medik Veteriner Journal of Parasite Science Ovozoa: Journal of Animal Reproduction Indian Journal of Forensic Medicine & Toxicology journal of Basic Medical Veterinary Jurnal Riset Rumpun Ilmu Hewani (JURRIH) PCJN Pharmaceutical and Clinical Journal of Nusantara Jurnal Agro Veteriner
Mafruchati, Maslichah
Division Of Veterinary Anatomy, Faculty Of Veterinary Medicine, Universitas Airlangga, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Earth & Planetary Sciences Health Professions Immunology & microbiology Medicine & Pharmacology Neuroscience Nursing Public Health Veterinary

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Tomato (Lycopersicon esculentum Mill.) juice restored the number of Leydig cells, and the diameter of the seminiferous tubules of mice (Mus musculus) exposed to lead acetate Revina Ayu Septiani; Iwan Sahrial Hamid; Emy Koestanti Sabdoningrum; Anwar Ma'ruf; Eka Pramyrtha Hestianah; Maslichah Mafruchati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.123-129

Abstract

Lead is a harmful pollutant from engine exhaust that causes free radicals and has detrimental effects on the testicular tissue. This study aimed to determine the effects of tomato juice on the number of Leydig cells and the diameter of the seminiferous tubules of mice exposed to lead acetate. Twenty-five male mice were divided into five groups. Mice in the control (C-) group were given placebos. Meanwhile, mice in C+, T1, T2, and T3 groups were exposed to lead acetate at a dose of 100 mg/kg BW/day for 14 days and given tomato juice respectively at 0, 0.16, 0.32, and 0.64 mL/day from day 8 to day 35. On day 36, all mice were sacrificed, and the testes were collected for histological preparation. The result showed that lead exposure in the C+ group decreased (p <0.05) in the number of Leydig cells and the diameter of the seminiferous tubules compared to the mice in group C-. Administration of tomato juice in groups T1, T2, and T3 increased (p <0.05) the number of Leydig cells and the diameter of seminiferous tubule compared to the mice in the C+ and C- groups. However, tomato juice administration to the T3 group decreased the number of Leydig cells and the diameter of the seminiferous tubules (p <0.05) compared to the T2 group. In conclusion, an effective dose of 0.32 mL/day of tomato juice restored Leydig cell number and seminiferous tubules diameter in mice exposed to lead acetate.
Total protein, albumin, and globulin levels of blood serum in repeat breeder Holstein Friesian cows Asrar, Siti Iqmallisa Nurrill; Rimayanti Rimayanti; Ismudiono Ismudiono; Maslichah Mafruchati; Gandul Atik Yuliani; Ginta Riady
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.90-98

Abstract

This study aimed to determine the relationship between total protein, albumin, and globulin in the blood with the incidence of repeat breeders in dairy cows. Ten normal cyclic cows and ten repeat breeders cows were taken randomly based on the reproductive records of the Tani Wilis Village Cooperative, Sendang District, Tulungagung Regency. Questionnaire data in the form of farmers' knowledge about dairy cows' reproductive systems and feed treatment is displayed descriptively.  Dairy cows blood samples were taken from the jugular vein 6 mL to measure total protein, albumin, and globulin levels. Data analyzed using the Statistical Product and Service Solution software at a significance level of 5%. The results showed that the total protein, albumin, and globulin levels in normal dairy cows and dairy cows with repeat breeders were not significantly different (p>0.05). However, in normal cyclic cows, most of the total protein, albumin, and globulin levels (80-100% of normal cyclic cows samples) are within the normal range. Meanwhile, the majority of repeat breeder cows (60-70% of the repeat breeder cows) had total protein, albumin, and globulin levels lower than the normal range (p<0.05). It could be concluded that there was a relationship between the incidence of repeat breeder and total protein, albumin, and globulin levels in the blood serum of dairy cows with repeat breeder. Therefore, it is suggested to raise awareness of smallholder farmers about proper feeding and feeding management to reduce repeat breeder cases.
Analysis of Storage Time for Merawang Chicken Eggs (Gallus Gallus) against Hatchability: A Systematic Review Mafruchati, Maslichah; Makuwira, Jonathan
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.80970

Abstract

Background: Merawang chicken has the potential to be developed, both as local laying hens and local broilers. Hatchability and quality of hatching eggs are influenced by storage method, storage time, storage area, ambient temperature, incubator temperature, reversal during hatching. Storage that is too long causes the quality and hatchability to decrease so eggs should be stored no more than 7 days. Purpose: The purpose of this study was to analyze the length of storage of Merawang chicken eggs (Gallus gallus) on hatchability. Research methods: A literature search was carried out systematically through the PubMed, NCBI, Google Scholar databases using keywords, namely, "Storage Period, Chicken Eggs, Merawang (Gallus Gallus), Hatchability". Based on these keywords, the articles obtained were first selected by setting several inclusion criteria. Research results: Based on the search results in the PubMed, NCBI, Google Scholar databases using predetermined keywords, 15400 articles were obtained for the keywords “Storage Time of Merawang Chicken Eggs (Gallus Gallus), Hatching Power”. All articles were reselected based on inclusion criteria and exclusion and obtained as many as 23 articles that met the inclusion criteria. Conclusion: From the results it can be concluded that the storage time is 9 days (P5) showed hatchability, high fertility and lowest embryo mortality. Need research on the other animal for future research.
Environmental and Management Risk Factors for Goat Mastitis in Banyuwangi Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati, Maslichah; Praja, Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.49399

Abstract

Mastitis is a persistent and economically significant health problem in dairy goats in Banyuwangi, East Java, where smallholder production systems dominate. This review synthesises current evidence on the primary risk factors contributing to the high prevalence of subclinical mastitis in the region. Local studies consistently report Staphylococcus aureus as the leading etiological agent, frequently exhibiting antimicrobial resistance linked to unsupervised antibiotic use. Host-related factors such as advanced parity, udder lesions, and teat-end damage increase susceptibility to intramammary infection. Management practices—including inadequate milking hygiene, use of shared towels, absence of pre- and post-dipping, and irregular equipment sanitation—facilitate the transmission of contagious pathogens. Environmental conditions, particularly high humidity and poor housing sanitation, further elevate infection pressure. Socio-economic constraints and limited farmer knowledge about subclinical mastitis and antibiotic stewardship exacerbate disease persistence. The multifactorial nature of mastitis in Banyuwangi underscores the need for context-appropriate interventions combining improved hygiene practices, better housing management, routine screening, and responsible antimicrobial use. Strengthened farmer education and targeted surveillance are essential to reduce mastitis prevalence and enhance the sustainability of goat dairy production in the region.
Environmental Impact Analysis on Chicken Embryo Development: A Systematic Review Fadhli Rahman Fauzi; Maslichah Mafruchati
JURNAL RISET RUMPUN ILMU HEWANI Vol. 4 No. 2 (2025): Oktober : JURRIH: JURNAL RISET RUMPUN ILMU HEWANI
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrih.v4i2.7935

Abstract

Environmental stress can affect the development of the embryo, including causing the death of the embryo. Environmental factors that can cause stress include salinity and zinc sulfate, zinc sulphate is a trace element and can affect embryonic development. The purpose of this study was to analyze the environment in Chicken embryonic development. A literature search was carried out systematically through the PubMed, NCBI, Google Scholar databases using keywords, namely, “heat, stress, embryo, chicken”. Based on these keywords, the articles obtained were first selected by setting several inclusion criteria. Articles that do not meet the inclusion criteria are eliminated, and articles that meet the criteria will be analysed to obtain data. Based on the search results 21326 articles were obtained, and 21 articles that meet the inclusion criteria were selected. The result above also showed that heat stress give impact to development of embryo. From the results of the research conducted, it can be concluded that the environment has an impact on the development of the embryo. Further studies are needed, especially those related to other types of stress that can affect embryonic development in various types of animals.
Chicken Embryo Model Perspective : Novel and Relevant Models Immunity-Based Research: A Systematic Review Fadhli Rahman Fauzi; Maslichah Mafruchati
JURNAL RISET RUMPUN ILMU HEWANI Vol. 4 No. 2 (2025): Oktober : JURRIH: JURNAL RISET RUMPUN ILMU HEWANI
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrih.v4i2.7936

Abstract

Dysregulation of the immune system is associated with many medical conditions, including: Cardiovascular disease, diabetes, cancer. Most popular model today. In biomedical research, rodents, despite the many advantages they offer, there are also many drawbacks to its use. Recently, another in vivo model, the chicken Embryos and their chorioallantoic membranes are resurfaced for a variety of uses. This model includes cost-effectiveness, time-efficiency, Easier to use. This review describes how to use chicken embryos. As a model for immune-based research because it gradually develops the embryonic immune system, Systems functionally similar to humans. This study examined mainly intended to describbe the immune system of birds, highlighting the differences and similarities with the human immune system A system containing a repertoire of lymphoid tissue, immune cells, and other important functions. A literature search was carried out systematically through the PubMed, NCBI, Google Scholar databases using keywords, namely, “Chicken embryo model, Perspective, Novel and relevant models Immunity, based research”. Based on these keywords, 21350 articles were obtained and 50 articles that meet the inclusion and exclusion criteria were selected. Those helped to describe general in-ovo immune ontogeny. Future studies are suggested to better tailor the use of chicken embryo models for testing carrying out specific experimental hypotheses or preclinical studies.
Crataegus Oxyacantha Based on Bioinformatics Studies and Toxicity Test on The Chicken Embryo Maslichah Mafruchati
JURNAL RISET RUMPUN ILMU HEWANI Vol. 4 No. 2 (2025): Oktober : JURRIH: JURNAL RISET RUMPUN ILMU HEWANI
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrih.v4i2.7938

Abstract

Hawthorn extract has been used for ameliorating cardiac disorders and pulmonary hypertension. The main chemical constituents of hawthorn flavonoid extract (HFE) include flavonoids (1-2%), oligomeric proanthocyanidins (1-3%), and other bioactive components (e.g., triterpene acids, organic acids, sterols, and cardioactive amines). These compounds are reported to have many pharmacological effects, including neuroprotective, hepatoprotective, cardioprotective, and nephroprotective effects. This study was aimed the analysis Crataegus oxyacantha based on a bioinformatic study and toxicity test on the chicken embryo. This method consisted of analysis of the three-dimensional structure of Crataegus oxyacantha Protein, Epitope and Allergen Proteins, Crataegus oxyacantha Proteins that were antigens and toxins and toxicity test on the chicken embryo. The results of research conducted on 3 three-dimensional structures of Crataegus oxyacantha protein, GQME value and QmeanDisCo value. In addition, this study also obtained the results of proteins that are epitope, antigenic, non-allergenic and non-toxic and toxicity test on the chicken embryo was 250 ng/egg..Morphological description of the embryo on the 21st day after injection, at a concentration of 250 ng of Crataegus oxyacantha /egg product, an abnormal embryological picture was obtained. Chicken Embryo Weight and Body Length Measurements were carried out in chicken embryos. Need research for other species of plant.
Achillea millefolium Based on Bioinformatics Studies and Toxicity Test on The Chicken Embryo Maslichah Mafruchati
JURNAL RISET RUMPUN ILMU HEWANI Vol. 4 No. 2 (2025): Oktober : JURRIH: JURNAL RISET RUMPUN ILMU HEWANI
Publisher : Pusat riset dan Inovasi Nasional

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55606/jurrih.v4i2.7939

Abstract

In Iranian Traditional Medicine, some herbs like Achillea millefolium, commonly known as yarrow, are implicated as appetite enhancers. However, there is not enough research evidence to prove their actual effect. Achillea millefolium is a dicot which belongs to the family Asteraceae. The purpose of this study was the analysis Achillea millefolium based on a bioinformatic study and toxicity test on the chicken embryo. This research method consisted of conversion of nucleotides into amino acids, analysis of the three-dimensional structure of Achillea millefolium protein, epitope and Allergen Proteins, antigens and toxins and toxicity test on the chicken embryo. In addition, this study also obtained the results of proteins that are epitope, antigenic, non-allergenic and non-toxic and toxicity test on the chicken embryo was 250 ng/egg.. Morphological description of the embryo on the 21st day after injection, at a concentration of 250 ng of Mentha piperita/egg product, an abnormal embryological picture was obtained. Chicken Embryo Weight and Body Length Measurements were carried out in chicken embryos. Need research for other species of plant.
Effect of Moringa oleifera Lam. Leaf Extract on Sperm Motility and Abnormality in White Rats (Rattus norvegicus) Exposed to Cigarette Smoke: An In Vivo Experimental Study Bachtiar, Harris Yudhanta; Mafruchati, Maslichah; Yudaniayanti, Ira Sari; Restiadi, Tjuk Imam; Utomo, Budi; Plumeriastuti, Hani
Jurnal Sain Veteriner Vol 43, No 3 (2025): Desember
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.106028

Abstract

Background: CSE has been shown to induce oxidative stress, negatively impacting sperm quality and morphology by decreasing motility and increasing abnormalities. Moringa oleifera Lam. leaves are rich in flavonoids and other antioxidants that may counteract the harmful effects of free radicals. Objectives: This study aimed to investigate the effect of Moringa oleifera leaf extract on sperm motility and abnormality in male white rats (Rattus norvegicus) exposed to cigarette smoke. Method: This true experimental study used a completely randomized design. Twenty-five male rats were divided into five groups: negative control (K−), positive control (K+), and three treatment groups (P1, P2, P3) receiving oral administration of Moringa leaf extract at graded doses (13, 26, and 52 mg/kg BW) for 14 days, along with daily exposure to cigarette smoke. Results: Results showed that group P3 (52 mg/kg BW) had significantly higher sperm motility compared to the positive control and was statistically comparable to the negative control (p<0.05). Furthermore, sperm abnormality in group P3 decreased significantly, approaching the normal range. Conclusion: In conclusion, the administration of Moringa oleifera leaf extract at a dose of 52 mg/kg BW effectively preserved sperm motility and reduced abnormality in white rats exposed to cigarette smoke. These findings support the potential of Moringa oleifera as a phytotherapeutic agent to protect reproductive health against oxidative stress.
Analysis of Embryo Development and Early Performance of Larvae of Barbonymus Schwanenfeldii: A Systematic Review Mafruchati, Maslichah; Makuwira, Jonathan
PCJN: Pharmaceutical and Clinical Journal of Nusantara Vol. 1 No. 01 (2022): PCJN: Pharmaceutical and Clinical Journal of Nusantara
Publisher : CV. Nusantara Scientific Medical

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (473.947 KB) | DOI: 10.58549/pcjn.v1i01.3

Abstract

Tengadak fish is a freshwater fish that can be found in rivers, lakes and canals and ditches. One of the important phases in fish development is the embryo. Embryo development (embryogenesis) is more in the process of formation and development of the embryo, not only the increase in the number and mass of embryonic blastomer cells but also the activity of blastomeric cells.The purpose of this study was to analyze the stages of embryonic development and early larval performance in Tengadak fish (Barbonymus schwanenfeldii). This study used systematic literature review using data from PubMed, NCBI, Google Scholar databases using keywords, namely, “embryo, performance, Tengadak fish (Barbonymus schwanenfeldii)”. Approximately, 350 articles were obtained for the keywords “embryo, performance, Tengadak fish (Barbonymus schwanenfeldii)”. All articles were selected based on inclusion criteria and exclusion and obtained as many as 27 articles that meet the inclusion criteria From the results of research conducted, it can be concluded that this research has sequentially described the embryonic development and early performance of the larvae of the barbonymus schwanenfeldii. Need research on the other animal for future research.
Co-Authors AA Sudharmawan, AA Adiana Mutamsari Witaningrum Al arif, Mohammad Anam Anugrah, Alvyan Lantang Anwar Ma'ruf Asrar, Siti Iqmallisa Nurrill Bachtiar, Harris Yudhanta Boedi Setiawan Budi Utomo Chusniati, Sri Dewi, Sapna Kurnia Eka Pramyrtha Hestianah Emy Koestanti Sabdoningrum Erma Safitri Fadhli Rahman Fauzi Gandul Atik Yuliani Ginta Riady Hana Eliyani Hani Plumeriastuti Hermin Ratnani IMAM MUSTOFA Ira Sari Yudaniayanti Ismudiono Ismudiono Iwan Sahrial Hamid Iwan Sahrial Hamid Kusnoto Kusnoto, Kusnoto Lestari, Dewi Sri Lokapirnasari, Widya Paramitha Lucia Tri Suwanti, Lucia Tri Luqman, Epy Muhammad Makuwira, Jonathan Meles, Dewa Ketut Mohammad Sukmanadi Mufasirin Muhammad Ilyas Wahyudi Nur, Maysarah Oktavia Praja, Ratih Novita Prastiya, Ragil Angga Rekasni Adallin A/P Morgan A/P Morgan Revina Ayu Septiani Rimayanti Rimayanti Rochmah Kurnijasanti Saputro, Amung Logam Setiawan Koesdarto Shabira, Zahra Sri Hidanah Sri Mulyati Sri Mumpuni Suherni Susilowati Tita Damayanti Lestari Tjuk Imam Restiadi Trilas Sardjito Tyasningsih, Wiwiek Viski Fitri Hendrawan Wahyudi, Nurul Tri Warsito, Sunaryo Hadi Widjiati Widjiati, Widjiati Yeni Dhamayanti