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Protection Effectiveness Of Bit (Beta vulgaris) Fruit Extract In Maintaining Spermatogenesis Ability In White Rats (Rattus novergicus) Induced Carbon Tetracloride (CCl4) Anugrah, Alvyan Lantang; Eliyani, Hana; Utomo, Budi; Susilowati, Suherni; Mafruchati, Maslichah; Kurnijasanti, Rochmah
Journal of Basic Medical Veterinary Vol. 10 No. 2 (2021): Journal of Basic Medical Veterinary, December 2021
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v10i2.31812

Abstract

The aim of this research was to know whether beetroot (Beta Vulgaris) extract could protect spermatogenesis by maintaining spermatogenic and sertoli cell count  in rats (Rattus novergicus) induced with CCl4. Rats were given beetroot extract daily, for 14 days and 3ml/kg BW CCl4 intraperitoneally one hour after last treatment. This study used twenty rats which were devided equally into 5 groups. K(-), the negative control group was not induced with CCL4 and only given 1% CMC-Na suspension. K(+), the positive control group was induced with CCl­4 and given 1% CMC-Na suspension. P1, P2 and P3 were given beet root extract with doses of 200, 400, and 800 mg/kg BW daily before feeding. All of the beetroot treatment were given orally (2 ml). After 24 hours CCl4 induction, rats were sacrificed and testis were collected to make histology slides. The observations showed significantly different (p<0,05) in all of variables. Result showed significant differences in spermatogenic and sertoli cells between K(-) and K(+) groups, K(+) with P2 and P3 group, and showed insignificant difference between P2 and P3 group in spermatogenic and sertoli cells. The result of this research showed that beetroot extract could protect the spermatogenic and sertoli cells in male rats induced with CCl4.
Isolation, Identification and Risk Factors of Escherichia coli Bacteria in Dairy Goat's Milk Subclinical Mastitis in Siliragung District, Banyuwangi Regency Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati , Maslichah; Praja , Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 12 No. 2 (2023): Journal of Basic Medical Veterinary, December 2023
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v12i2.49401

Abstract

Mastitis is an inflammation of the mammary gland which can cause a decrease in milk production and income for farmers. Subclinical mastitis is mastitis without clinical symptoms but can result in a decrease in the quality of milk production. One of the main pathogens that cause mastitis is Escherichia coli. The risk factors for the occurrence of mastitis, both clinical and subclinical mastitis, can cause a decrease in milk production caused by several factors, including genetic, feed, and environmental factors. This study aims to determine the presence of subclinical mastitis in dairy goat milk in Siliragung District, Banyuwangi Regency. Sample testing was carried out at the SIKIA Instrument Laboratory, Airlangga University. Isolation was carried out using EMBA media (Eosin Methylene Blue Agar) for further identification consisting of Gram staining and biochemical tests. One of the biochemical tests used is the IMViC (Indole Methyl red Voges-Proskauer Citrate Test). The results of isolation, observation of characteristics, and identification of bacteria in the research that was carried out showed that 8% (5/62) of the samples tested were positively identified as Escherichia coli. The results showed that the risk factors for causing subclinical mastitis included cleaning the cage bedding, washing hands before milking, milking out, teat dipping, and medication.
Role of Cryoprotectants in Preventing Quality Loss of Goat Oocytes after Warming by Examining Insulin-Like Growth Factor-1 and Bone Morphogenetic Protein-15 Expression Shabira, Zahra; Widjiati, Widjiati; Rimayanti, Rimayanti; Lestari, Tita Damayanti; Safitri, Erma; Mafruchati, Maslichah; Hendrawan, Viski Fitri
Jurnal Medik Veteriner Vol. 8 No. 1 (2025): April
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jmv.vol8.iss1.2025.175-182

Abstract

This research aimed to assess the efficacy of ethylene glycol-sucrose as an alternative cryoprotectant compared to commercial cryoprotectants by focusing on the expression of insulin-like growth factor-1 (IGF-1) and bone morphogenetic protein-15 (BMP-15) in goat oocyte cumulus complexes after warming with identification using the immunocytochemical analysis. Maturated oocytes were divided into 3 groups, i.e., (C) the control group followed by an immunocytochemical examination, and two treatment groups, i.e., (P1) was exposed to a commercial cryoprotectant for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen, and (P2) was exposed to 30% ethylene glycol and 1 M sucrose for 15 minutes, then placed in a hemistraw and dipped in liquid nitrogen. The results revealed divergent responses in IGF-1 (CG = 9.00 ± 3.00; P1 = 9.50 ± 3.20; P2 = 4.67 ± 0.94) and BMP-15 (CG = 10.50 ± 3.35; P1 = 9.50 ± 2.69; P2 = 5.50 ± 3.64) expression patterns, possibly influenced by the two cryoprotectant abilities and oocytes performing their permeabilities to cryoprotectant solution. We concluded that each cryoprotectant is necessary to mitigate the adverse impact on gene expression in oocytes so that we can use it for reproductive technologies.
Profil bakteri non spesifik dalam lendir serviks sapi perah pada fase folikuler dan fase luteal Nur, Maysarah Oktavia; Mulyati, Sri; Sardjito, Trilas; Chusniati, Sri; Tyasningsih, Wiwiek; Mafruchati, Maslichah
Ovozoa: Journal of Animal Reproduction Vol. 9 No. 1 (2020): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (830.154 KB) | DOI: 10.20473/ovz.v9i1.2020.17-22

Abstract

This research was conducted to identify non-specific bacteria found in the cervical mucus of dairy cattle in the follicular and luteal phase. Twenty cervical mucus samples were collected from ten cows during their follicular phase and ten cows during their luteal phase. Sampling was carried out with the help of an artificial insemination gun and a plastic sheath, which were protected by a sheath cover. After insertion into cervix, the tip of the plastic sheath was cut and put into a tube prefilled with phosphate buffered saline. All samples were cultured in Blood Agar media and isolated in Eosin Methylene Blue Agar, Mac Conkey Agar, and Manitol Salt Agar. Gram staining was applied to distinguish gram-positive and gram-negative bacteria; and followed with the catalase, spore, motility, and biochemical tests to further identify bacteria. The bacteria identified were of the genus Enterobacter, Staphylococcus, Corynebacterium, Streptococcus, Escherichia, and Pasteurella. It could be concluded that non-specific bacteria of the same genus were identified in cervical mucus of dairy cows in their follicular and luteal phase, in a various of percentages.
Tomato (Lycopersicon esculentum Mill.) juice restored the number of Leydig cells, and the diameter of the seminiferous tubules of mice (Mus musculus) exposed to lead acetate Revina Ayu Septiani; Iwan Sahrial Hamid; Emy Koestanti Sabdoningrum; Anwar Ma'ruf; Eka Pramyrtha Hestianah; Maslichah Mafruchati
Ovozoa: Journal of Animal Reproduction Vol. 11 No. 3 (2022): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v11i3.2022.123-129

Abstract

Lead is a harmful pollutant from engine exhaust that causes free radicals and has detrimental effects on the testicular tissue. This study aimed to determine the effects of tomato juice on the number of Leydig cells and the diameter of the seminiferous tubules of mice exposed to lead acetate. Twenty-five male mice were divided into five groups. Mice in the control (C-) group were given placebos. Meanwhile, mice in C+, T1, T2, and T3 groups were exposed to lead acetate at a dose of 100 mg/kg BW/day for 14 days and given tomato juice respectively at 0, 0.16, 0.32, and 0.64 mL/day from day 8 to day 35. On day 36, all mice were sacrificed, and the testes were collected for histological preparation. The result showed that lead exposure in the C+ group decreased (p <0.05) in the number of Leydig cells and the diameter of the seminiferous tubules compared to the mice in group C-. Administration of tomato juice in groups T1, T2, and T3 increased (p <0.05) the number of Leydig cells and the diameter of seminiferous tubule compared to the mice in the C+ and C- groups. However, tomato juice administration to the T3 group decreased the number of Leydig cells and the diameter of the seminiferous tubules (p <0.05) compared to the T2 group. In conclusion, an effective dose of 0.32 mL/day of tomato juice restored Leydig cell number and seminiferous tubules diameter in mice exposed to lead acetate.
Total protein, albumin, and globulin levels of blood serum in repeat breeder Holstein Friesian cows Asrar, Siti Iqmallisa Nurrill; Rimayanti Rimayanti; Ismudiono Ismudiono; Maslichah Mafruchati; Gandul Atik Yuliani; Ginta Riady
Ovozoa: Journal of Animal Reproduction Vol. 12 No. 2 (2023): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v12i2.2023.90-98

Abstract

This study aimed to determine the relationship between total protein, albumin, and globulin in the blood with the incidence of repeat breeders in dairy cows. Ten normal cyclic cows and ten repeat breeders cows were taken randomly based on the reproductive records of the Tani Wilis Village Cooperative, Sendang District, Tulungagung Regency. Questionnaire data in the form of farmers' knowledge about dairy cows' reproductive systems and feed treatment is displayed descriptively.  Dairy cows blood samples were taken from the jugular vein 6 mL to measure total protein, albumin, and globulin levels. Data analyzed using the Statistical Product and Service Solution software at a significance level of 5%. The results showed that the total protein, albumin, and globulin levels in normal dairy cows and dairy cows with repeat breeders were not significantly different (p>0.05). However, in normal cyclic cows, most of the total protein, albumin, and globulin levels (80-100% of normal cyclic cows samples) are within the normal range. Meanwhile, the majority of repeat breeder cows (60-70% of the repeat breeder cows) had total protein, albumin, and globulin levels lower than the normal range (p<0.05). It could be concluded that there was a relationship between the incidence of repeat breeder and total protein, albumin, and globulin levels in the blood serum of dairy cows with repeat breeder. Therefore, it is suggested to raise awareness of smallholder farmers about proper feeding and feeding management to reduce repeat breeder cases.
Analysis of Storage Time for Merawang Chicken Eggs (Gallus Gallus) against Hatchability: A Systematic Review Mafruchati, Maslichah; Makuwira, Jonathan
Jurnal Sain Veteriner Vol 42, No 2 (2024): Agustus
Publisher : Faculty of Veterinary Medicine, Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.80970

Abstract

Background: Merawang chicken has the potential to be developed, both as local laying hens and local broilers. Hatchability and quality of hatching eggs are influenced by storage method, storage time, storage area, ambient temperature, incubator temperature, reversal during hatching. Storage that is too long causes the quality and hatchability to decrease so eggs should be stored no more than 7 days. Purpose: The purpose of this study was to analyze the length of storage of Merawang chicken eggs (Gallus gallus) on hatchability. Research methods: A literature search was carried out systematically through the PubMed, NCBI, Google Scholar databases using keywords, namely, "Storage Period, Chicken Eggs, Merawang (Gallus Gallus), Hatchability". Based on these keywords, the articles obtained were first selected by setting several inclusion criteria. Research results: Based on the search results in the PubMed, NCBI, Google Scholar databases using predetermined keywords, 15400 articles were obtained for the keywords “Storage Time of Merawang Chicken Eggs (Gallus Gallus), Hatching Power”. All articles were reselected based on inclusion criteria and exclusion and obtained as many as 23 articles that met the inclusion criteria. Conclusion: From the results it can be concluded that the storage time is 9 days (P5) showed hatchability, high fertility and lowest embryo mortality. Need research on the other animal for future research.
Environmental and Management Risk Factors for Goat Mastitis in Banyuwangi Lestari, Dewi Sri; Setiawan, Boedi; Saputro, Amung Logam; Mafruchati, Maslichah; Praja, Ratih Novita; Prastiya, Ragil Angga
Journal of Basic Medical Veterinary Vol. 14 No. 2 (2025): Journal of Basic Medical Veterinary, December 2025
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/jbmv.v14i2.49399

Abstract

Mastitis is a persistent and economically significant health problem in dairy goats in Banyuwangi, East Java, where smallholder production systems dominate. This review synthesises current evidence on the primary risk factors contributing to the high prevalence of subclinical mastitis in the region. Local studies consistently report Staphylococcus aureus as the leading etiological agent, frequently exhibiting antimicrobial resistance linked to unsupervised antibiotic use. Host-related factors such as advanced parity, udder lesions, and teat-end damage increase susceptibility to intramammary infection. Management practices—including inadequate milking hygiene, use of shared towels, absence of pre- and post-dipping, and irregular equipment sanitation—facilitate the transmission of contagious pathogens. Environmental conditions, particularly high humidity and poor housing sanitation, further elevate infection pressure. Socio-economic constraints and limited farmer knowledge about subclinical mastitis and antibiotic stewardship exacerbate disease persistence. The multifactorial nature of mastitis in Banyuwangi underscores the need for context-appropriate interventions combining improved hygiene practices, better housing management, routine screening, and responsible antimicrobial use. Strengthened farmer education and targeted surveillance are essential to reduce mastitis prevalence and enhance the sustainability of goat dairy production in the region.