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Wartono Wartono
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian Jl. Tentara Pelajar 3A Bogor 16111
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Journal : BERITA BIOLOGI

 1 
SELEKSI JAMUR PATOGEN SERANGGA Beauveria spp. SERTA UJI PATOGENISITASNYA PADA SERANGGA INANG-WALANG (Leptocorisa acuta) [Selection of Enthomopathogenic Fungi Beauveria spp. and their Pathogenicity Test Against Insect Host-Rice Stink Bug (Leptocorisa acuta)] Wartono, Wartono; Nirmalasari, Cyntia; Suryadi, Yadi
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3848.189 KB) | DOI: 10.14203/beritabiologi.v15i2.2271

Abstract

Rice-stink bug (Leptocorisa acuta Thumb) that attack rice crop often causing heavy damage of panicle of rice as well as decreasing either quantity or quality of grain after harvest. The objective of this research was to select 14 entomopatogenic fungi of Beauveria spp. isolates, collected from rice stink bug (L. acuta) and their pathogenicity assay to insect host L. acuta. We also aimed to study diversity of Beuveria spp. which was isolated from insect host from Situgede, Bogor. The research was conducted at Laboratory, and Green house of Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor, in 2014. Result of the study showed that isolates from Situgede Bogor were sucessfully isolated  as Beauveria spp. based on Koch Postulat assay.  The identified fourteen isolates were morphologically confirmed as Beauveria spp. The pathogenicity test was indicated by symptoms and mortality of rice stink bug after inoculation with these entomopathogenic fungi. The Beuveria isolates Stgd2(14)1, Stgd6(14)1, Stgd7(14)2, Stgd8(14)2, and Stgd0113 were the most virulent isolates. Stgd2(14)1 provided the fastest time to kill L. acuta with LT50 values of 6.9 days. The 13 isolates of Beauveria were well amplified by ITS primers. However, no diversity was found among isolates, presumably due its narrow host range tested. 
Co-Authors Agus Sarifudin, Agus Agustina, Annisa Ainul Yaqin alfi husni, alfi Alfiana Alfiana Alfroni, Yohana Fransiska Ali Maulida, Ali Amelia, Sifa Andani, Hamdan Anindya, Regita Ariansyah, Arif Arijulmanan, Arijulmanan Arisandi, Oriza Asmawati Asmawati Asrofiani, Dwi Azhari, Lukman Batlolona, John Rafafy Bimasri, Jhon Bimasri, John Danarti Karsono Desella Inna Rahmatina, Desella Inna Donita, Salwa Audriana DWI NINGSIH SUSILOWATI Dwicahya , Nadya Elfira Safitri Elmarma, Fazlur Rahman Erison, Yendra Erni Widarti Etty Safriyani Faiz Muqorrir Kaaffah Farida Farida Fatkhurohman, Irfan Fauzi Ahmad Muda Fitri Amalia Grusche, Sascha Hadi, Izzul Havidz Cahya Pratama Hermanto Holidi Holidi Indera, Edi Indrawan, Mohamad Gita Irma Suryani Iskandar Iskandar Jayanti, Y. M. Jayanti, Y. M. Khofid, Adi Nur Komalasari, Nyai Lestari, Ega Lia Yuliati, Lia Lifianthi, Lifianthi Mahapoonyanont, Natcha Malik, Akmal Moh. Erkamim Mohammad Soleh Muhafzan Muhafzan Muhammad Arif Muhammad Hidayat Ginanjar, Muhammad Hidayat Muhammad Nur Hudha Muspida, Muspida Nazvira, Mutia Nirmalasari, Cyntia Nirmalasari, Cyntia Pamungkas, Muh. Rizal F. Pamungkas, Muhammad Rizal Fernandita Panusunan, Panusunan Permatasari, Lintang Ayu Pratama, Rahmadhani Prayitno, M. Hadi Puji Hapsari, Elsa PUJI LESTARI Purwasih, Hari Dewi Puspita, Alisa Zulia Putri, Rahmadhani Yulmi Rahendra Maya, Rahendra Rahman Rahman Rakhadani, Ihrom Ramadhani, Argia Putri Rasid, Husni Muhammad Rinda Kirana Riry Sriningsih Rizal Fernandita Pamungkas, Muhammad Rohmah, Eva Nur Laily Roni, Roni Rudiansyah Rundi, Allesandro U.B. Rundi, Allesandro Umbu Balla Saifuddin Saifuddin Sandi, Elisa Harlia Santi, Neng Sentot Kusairi Septiana, Ambar Setiawan, Hamdan Setyawati, Nisrina Yulia Sholihuddin, M Nur Siti Fatimah Azzahra SOLAHUDIN SOLAHUDIN, SOLAHUDIN Sulasiwi, Intan Febry Sumarjono Sumarjono Sumini Sumini, Sumini Sumpena, Sumpena Supriyono Koes Handayanto Supriyono Supriyono Suryani Suryani Susanti, Rini Puji Sutopo Sutopo Syahputra, Ridwan Angga Takaria, Johannis Takaria, Johannis Tri Wulandari, Fera Trianto, Joko Ujang Andi Yusuf, Ujang Andi Unang Wahidin, Unang Wati, Dewi Gislia Wati, Vera Yadi Suryadi Yasyakur, Moch Yuanita FD Sidabutar Yuslenita Muda Zakaria, Aceng